4 bioreactors for engineering of structural tissue

... of cartilage-like tissue made by tissue engineering in the treatment of cartilage defects of the knee J Bone Joint Surgr 2002, 84:571-578 Shieh SJ, Terada S, Vacanti JP: Tissue engineering auricular ... concepts in tissue engineering J Biomed Mater Res 1998, 43:422-427 Isogai N, Landis W, Kim TH, Gerstenfeld LC, Upton J, Vacanti JP: Formation of phalanges and small joints by tissue -engineering ... mechanical support for the seeded cells and their developing matrix until the polymer is remodeled by the host tissue [21] Therefore, the clinical application of cell/scaffold complexes for cartilage...

Ngày tải lên: 09/08/2014, 06:22

... using light microscopy under different magnifications New bone formation (NBF), fibrous tissue formation (FTF), cartilage tissue formation (CTF) was assessed histomorphometrically using AnalySIS ... vessel formation were common findings in all groups It was found that both materials initiated foreign body reactions; however, this was more prominent for β – TCP There were also fibrous tissue ... new bone tissue in spite of the grafted sites In β – TCP groups, the residual bone graft particles were surrounded by a combination of newly formed bone and cartilage tissue The new bone formation...

Ngày tải lên: 25/10/2012, 11:15

... pretreated with PD98059 (50 lM) for 90 and thereafter treated with EGF for 48 h They were then exposed to 20 lM ADR for h, incubated for h in ADR-free medium, and harvested for immunoblot analysis of ... poly(dI-dC)Æpoly(dI-dC)] For supershift experiments, AP1 factor-specific antibodies were incubated with the binding reaction mixture for 30 at room temperature before addition of the 32 P-labeled oligonucleotide For ... Cells were treated in the presence or absence of EGF for 48 h and exposed to ADR for h They were then incubated in ADR-free medium and harvested for northern blot analysis of Bcl-XL mRNA Times after...

Ngày tải lên: 19/02/2014, 06:20

... metal droplets then preferentially formed Wire growth from an ordered array of gold droplets was successfully performed Acknowledgement The authors thank T Remmele for the TEM measurements References ... concentration of about 25 mol% For this concentration, Naidich et al [12] found a surface tension of about 1.0 J/m2 at 1500 1C No data could be found in the literature for the surface tension of ... tension tend to form droplets with a small contact area with the underlying substrate whereas liquids with lower surface tension tend to wet the substrate This could explain the formation of smaller...

Ngày tải lên: 16/03/2014, 15:17

... that performs segmentation of the bone and soft tissue for each CT slice, subsequently creating corresponding regions of interest The regions of interest are copied to the registered reformatted ... specific gravity of the tissue For example: we showed that the mean FB in the RUL is 6.16 ml blood per minute per 100 ml bone tissue If the specific gravity of bone is (for example) 1.8 gr/ml, ... leg and 10 cm distal to the olecranon tip in the forearm) and was inflated to 45 mm Hg for seconds to prevent venous egress During this period, forearm volume changes per time unit (correlates...

Ngày tải lên: 20/06/2014, 01:20

... OPCs in GCTs Therefore, it is conceivable that VEGF RTK inhibitors may be a useful clinical therapeutic for GCTs Acknowledgements This work was supported by a Grant-in Aid for Scientific Research ... Research (19390397 and 19791036) from the Japan Society for the Promotion of Science, Grantsin-aid for Clinical Research Evidenced Based Medicine, and for Cancer Research from the Ministry of Health, ... were initially decalcified for two weeks in an EDTA-containing buffer and embedded in paraffin The endogenous peroxidase activity was quenched by incubating the sections for an additional 30 in...

Ngày tải lên: 20/06/2014, 04:20

... OPCs in GCTs Therefore, it is conceivable that VEGF RTK inhibitors may be a useful clinical therapeutic for GCTs Acknowledgements This work was supported by a Grant-in Aid for Scientific Research ... Research (19390397 and 19791036) from the Japan Society for the Promotion of Science, Grantsin-aid for Clinical Research Evidenced Based Medicine, and for Cancer Research from the Ministry of Health, ... were initially decalcified for two weeks in an EDTA-containing buffer and embedded in paraffin The endogenous peroxidase activity was quenched by incubating the sections for an additional 30 in...

Ngày tải lên: 20/06/2014, 07:20

... Italy)) Thirty PCR cycles were performed at 94°C for 30 s, 55°C for 30 s and 72°C for min, after a first denaturing cycle at 94°C for A final extension cycle of was performed at 72°C PCR products were ... administration of an informed consent form No information was available on the first (I) generation (subjects I-1, I-1.0 and I-2; Fig 1) In the second (II) generation (Fig 1) blood samples for genomic DNA ... purified sequence was then resuspended in 15 µl of formamide and denatured for at 95°C Analysis of the forward and reverse sequences was performed on an ABI Prism 3100 Genetic Analyzer (Applied...

Ngày tải lên: 09/08/2014, 07:20

... until analyzed Synovial tissue samples for in situ hybridization were embedded in OCT Tissue Tek (Miles, Elkhart, IN, USA) before being frozen Synovial tissue samples for immunohistochemistry ... characteristics and age and gender for controls are given in Table No further information about the controls was made available for ethical reasons Tissue samples for mRNA analysis by microarrays ... reactions were performed with AmpliTaq Gold Mix (Perkin Elmer, Weiterstadt, Germany) in a reaction volume of 80 µl, amplifying at 93°C for minute, 62°C for minute, and 72°C for minutes For quantification...

Ngày tải lên: 09/08/2014, 07:20

... Abingdon, UK) or 100 nmol/ l cortisol or cortisone for 24 hours before harvesting Immunohistochemistry Cryostat tissue sections of synovial tissue from patients with RA were analyzed using goat ... were labelled with VIC Reactions occurred as follows: 50°C for minutes, 95°C for 10 minutes, 44 cycles of 95°C for 15 seconds and 60°C for minutes Data were obtained as Ct values (the cycle number ... iU), or IL-4 (10 ng/ml) for 24 hours Total RNA from these cells was then used to assess expression of mRNA for 11βHSD1 In each case mRNA levels for 11β-HSD1 were normalized for the housekeeping...

Ngày tải lên: 09/08/2014, 08:22

... Conditioning was performed for an additional 24 h in serum free Ham F12/DMEM media Confluent cells were incubated or not with human IGF-1 (50 ng/ml; Peninsula, Belmont, CA, USA) for different times ... first incubated for minutes at 50°C (UNG reaction), then at 95°C for 15 minutes (UNG inactivation and polymerase activation) followed by 40 cycles consisting each of denaturation (94°C for 15 s), ... consisting each of denaturation (94°C for 15 s), annealing (60°C for 30 s), extension (72°C for 30 s) and data acquisition (77°C for 15 s) steps The data were collected and processed with the GeneAmp...

Ngày tải lên: 09/08/2014, 08:23

... control for the device was performed by daily assessment of a spine phantom The in vivo precision error for dual-energy X ray absorptiometry, expressed as a Page of (page number not for citation ... College of Rheumatology criteria for RA [11] and gave informed consent to participate in this study, which was approved by the ethics committee This study was performed by the investigators, independent ... and at year for BMD, DAS28, osteocalcin, CTX-I, PTH, 25-OHD, erythrocyte sedimentation rate and C-reactive protein Data for both groups were compared using the unpaired Student's ttest for continuous...

Ngày tải lên: 09/08/2014, 10:20

... undergoing hip arthroplasty for primary OA and from 10 patients (10 females [74 to 87 years]) undergoing hip arthroplasty for a fractured neck of femur (designated OP) For the control group, trabecular ... samples in each pair for each group of paired samples to balance for potential dye incorporation and signal intensity bias It also reduced the number of slides required for the experiment and ... The reactions were incubated at 94°C for 10 for cycle, and then 94°C (20 seconds), 60°C, or 65°C (ADAMTS4 and MMP25 only; 20 seconds) and 72°C (30 seconds) for 40 cycles This set of cycles was...

Ngày tải lên: 09/08/2014, 10:21

... and 12 months of age to collect trunk blood For histology, mammary tissue from 3, and 12 month old mice was removed post mortem and formalin fixed for histology and stained with hematoxylin and ... incubated for hr at RT followed by blocking with 0.001% BSA, washing with diH2O and drying with N gas For the assays, a baseline impedance value for the circuit was obtained using PBS for 30 sec, ... depicted in Figure 3, panel A The device uniformity was not optimized for entire wafers, but individual circuits used for the assays were carefully evaluated before the experiment The selected CNT-FET...

Ngày tải lên: 11/08/2014, 00:23

Ngày tải lên: 13/08/2014, 01:20

... chemotherapy regimens Therefore, there is a critical need for new ATL therapies with improved efficacy over current treatments http://www.retrovirology.com/content/3/1/22 HTLV-1-transformed T-cell lines, ... specific for Jak-Stat pathway To determine whether AG490 inhibits DNA binding activity of Stat3 and Stat5 in HTLV-1-infected T-cell lines, we treated the cells with 50 µM AG490 for 24 h and performed ... Jak-Stat is responsible for the proliferation and survival of ATL cells The mechanism for the constitutive activation of Jak-Stat after HTLV-1 infection is still unclear HTLV-1 transforming protein Tax...

Ngày tải lên: 13/08/2014, 09:21

... nonsepsis patients, would be desirable for future studies to elucidate whether observed changes are specific for the septic setting Page of (page number not for citation purposes) Critical Care ... the bloodstream; these migrate to the site of infection for eradication of the pathogen (by proteolysis of the basement membrane) and for modulating the inflammatory response [3] The action of ... Available online http://ccforum.com/content/13/5/R158 penia (platelet count less than 100,000/μl), hyperbilirubinemia (plasma total bilirubin more than mg/dl) Five Tissue perfusion parameters:...

Ngày tải lên: 13/08/2014, 19:20

... Catch per unit of effort (CPUE) was calculated individually for each sampling site as the number of lingcod caught per angler per hour fishing To improve consistency in sampling effort across days ... heterogeneously distributed resource For instance, Fretwell (1972) proposed the ideal despotic distribution (IDD) for territorial species, in which the suitability of a habitat patch for an individual declines ... new settlers will be forced into lower quality patches Importantly, the presence of new individuals does not reduce patch quality for early settlers (Sutherland 1996) Thus, for territorial species...

Ngày tải lên: 04/09/2015, 17:15

... composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering Accepted in Bone Fig.3b was selected for cover-art for July issue of Bone Bone, Vol 45, 4–16, 2009 Impact factor: ... 35 I 2.2.3 Tissue- engineered bone grafts 39 2.2.3.1 Types of polymers used for tissue- engineered bone grafts 40 2.2.3.2 Types of n-HA/Collagen-based composites used for tissue- engineered ... growing on mineralized nanofibers (a) PLLA+ n-HA for day; (b) PLLA+ n-HA for days; (c) PLLA+ n-HA for days; (d) PLLA/Col+ n-HA for day; (e) PLLA/Col+ n-HA for days Fig 3.8: Total protein assay of cells...

Ngày tải lên: 11/09/2015, 09:57