3 min assays for cytotoxicity and phosphatase activity

Ngày tải lên: 21/02/2014, 09:20

... Vps4pE 233 Q Vps4pE 233 QDEL Vps4pE 233 Q Vps4pE 233 QDEL Vps4pE 233 QTRP Vps4pE 233 QRDF Vps4pE 233 QTRP Vps4pE 233 QRDF C 24 °C, days 40 °C, days 40 °C, 11 days Vps4p-WT empty vector Vps4p-E 233 Q Vps4p-E 233 Q-DEL ... C-terminal yEGFP tag YCplac111 expressing Vps4p–RDF with a C-terminal yEGFP tag YCplac111 expressing Vps4p–E 233 Q, D394 -39 9 (Vps4p–E 233 Q-DEL) YCplac111 expressing Vps4p–E 233 Q, D4 13- 424 (Vps4p–E 233 Q-TRP) ... expressing Vps4p–E 233 Q, D 430 - 437 (Vps4p–E 233 Q–RDF) pET11a E coli expression vector expressing Vps4p–RDF with a C-terminal 6His tag pET11a expressing Vps4p–E 233 Q, D 430 - 437 (Vps4p–E 233 Q–RDF) with a...

Ngày tải lên: 07/03/2014, 05:20

... individually, resulting in HspH(I 133 A) and HspH(I 135 A), or simultaneously, leading to HspH(II 133 , 135 AA) Finally, we searched for the minimal fragment required for dimer formation For this purpose HspH variants ... Chaperone activity assay Plasmids for the expression of B japonicum hspF (pRJ 530 6) and hspH (pRJ 530 7) provided with a C-terminal His6 tag have been described previously [ 13] N- or C-terminal truncations ... chaperone activity and oligomer formation, we replaced the two isoleucine residues in HspH by alanines and analyzed whether these mutations had a similar effect In fact, HspH(I 133 A), HspH(I 135 A) and...

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 28/06/2014, 23:48

... EXERCISES Key vocabulary Look up the meaning and pronunciation of these words in your dictionary reservation moment quiet facing lovely rear together I don’t mind lobby view not really like Grammar ... of the hotel The swimming pool is the sixth floor There’s some meat the fridge You will find the car park _ the rear of the hotel I can put you rooms six-eleven and six-twelve The lifts ... see what I can Answers: 1) at 2) on 3) in 4) at 5) in 6) on 1) Would you like someone to help you with your suitcases? 2) Is there anything else I can do? 3) Do you need any help? to like suitcases...

Ngày tải lên: 05/11/2012, 09:52

... A) B) C) D) E) the the the the the writer and television writer and his brother writer and telephone writer's brother and book writer's brother and meal The writer's brother wants him to A) ... as 'home - helps' for old people unable to look after themselves and who are living on their own These machines can now carry out such things as cook eggs and clean the floor, and the company says ... suitable form of the words defined above Life in a small village could be deadly dull, and Henry had been absolutely when he chose to be Sudden changes of from cheerful and alert to sullen and moody...

Ngày tải lên: 23/12/2013, 11:15

... piece of flip-chart paper Activity 2 .3: Identifying the Business Challenge and Vision Statement 11 ! Develop a common business challenge for the entire class (10 minutes) Choose an individual ... exercise and future activities 12 Activity 2 .3: Identifying the Business Challenge and Vision Statement Exercise 2: Developing the Vision Statement ! Develop the vision statement (15 minutes) ... 10 Activity 2 .3: Identifying the Business Challenge and Vision Statement Exercise 1: Identifying the Business Challenge ! State the business challenge (15 minutes) Participate...

Ngày tải lên: 24/01/2014, 10:20

... 73. 2 31 .4 26.2 57.6 Denmark 1992 39 .7 12.5 52.2 26 11 .3 37 .3 England2 20 03 43. 2 22.2 65.4 32 .6 23 55.6 Estonia (self reported) 1994-8 35 .5 9.9 45.4 26.9 15 .3 42.2 Finland 1997 48 19.8 67.8 33 ... reported) 20 03 37.4 11.4 48.8 23. 7 11 .3 35 Germany 2002 52.9 22.5 75.4 35 .6 23. 3 58.9 Greece 1994-8 51.1 27.5 78.6 36 .6 38 .1 74.7 Hungary 1992-4 41.9 21 62.9 27.9 21.2 49.1 Ireland 1997-99 46 .3 20.1 ... 46 .3 20.1 66.4 32 .5 15.9 48.4 Italy (self reported) 1999 41 9.5 50.5 25.7 9.9 35 .6 Latvia 1997 41 9.5 50.5 33 17.4 50.4 Lithuania 1997 41.9 11.4 53. 3 32 .7 18 .3 51 45.6 15 .3 60.9 30 .7 13. 9 44.6 Luxembourg...

Ngày tải lên: 14/02/2014, 13:20

... in single, human platelets Regulation by 38 4 30 31 32 33 34 35 36 37 38 39 40 41 modulation of the inositol trisphosphate receptors Eur J Biochem 269, 15 43 1552 Fischer L, Gukovskaya AS, Young ... phospholipase C and PI3-K activity PI3-Kb and not PI3-Kc mediates the P2Y12 effect on thrombin-evoked Ca2+ responses In man and mouse, the PI3-Kb (p110b) and PI3-Kc (p110c) isoforms are involved ... Sharp and Dohme (Haarlem, the Netherlands); Akt inhibitor and LY294002 from Calbiochem (La Jolla, CA, USA) ET-18-OCH3, manoalide, U 731 22 and U 733 43 came from Biomol (Plymouth Meeting, PA, USA) PI3-K...

Ngày tải lên: 18/02/2014, 16:20

... adhesion J Peptide Res 53, 18–29 Sutcliffe, M.J., Structure determination from, NMR data II Computational approaches In NMR of Macromolecules: a 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Practical ... adhesion and activation via the CD2 pathway Int Immunol 3, 133 5– 134 7 Mosmann, T (19 83) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays ... kcalÆmol)1) CD58 Residue Atom Residue Atom Ser5 Phe11 Ser5 Arg3 Ser5 Thr6 Arg3 Phe11 Arg3 Hc O HN O O O NH1 O He Lys30 Lys30 Gln31 Gln31 Asp 33 Asp 33 Ser69 Ser70 Glu72 O Hf Od He HN Hd O Hc Oe which was...

Ngày tải lên: 19/02/2014, 13:20

... Tables 129 129 129 131 133 133 135 137 137 138 138 138 139 139 139 139 140 140 140 140 141 141 142 147 156 156 156 157 158 158 159 159 159 160 161 161 161 162 162 169 172 172 172 1 73 174 176 177 177 ... populations D Summary E Gaps in the Literature F Implications 6 11 12 17 20 23 23 23 24 25 26 27 27 28 36 36 38 39 40 42 42 43 43 44 44 45 55 60 60 60 61 61 61 62 62 67 69 70 70 71 Research recommendations ... understanding of the links between physical activity and the health and well-being of girls and women A Project Purpose and Limitations The Health Benefits of Physical Activity for Girls and Women...

Ngày tải lên: 05/03/2014, 13:20

... of moderate activity For example, 150 minutes of moderate activity, 75 minutes of vigorous activity, and a combination of 100 minutes of moderate activity plus 25 minutes of vigorous activity all ... enjoyable, and accessible environments for physical activity in schools and workplaces, and for transportation and recreation in communities ACS Guidelines for Nutrition and Physical Activity About ... work, and schools, and decrease access to and marketing of foods and drinks of low nutritional value, particularly to youth • Provide safe, enjoyable, and accessible environments for physical activity...

Ngày tải lên: 06/03/2014, 02:21

... 14, 14, and lm were calculated for the wild-type protein and for the Ser16Ala, Ser127Ala and Ser16AlaSer127Ala mutant proteins, respectively These results demonstrated that none of the amino acid ... rate of chorismate formation was six- and 70-fold lower for the Ser127Ala and Ser16Ala mutant proteins, respectively, than for the wild-type enzyme, as shown in Table The residual activity of the ... primer, 5¢-CGACTTGCAGTGCGCCTCGC CATAGGTCG -3 ; S127A, forward primer, 5¢-GCGGCCG CTCTGCCGCCCGCGAGACC -3 , and reverse primer, 5¢-GGTCTCGCGGGCGGCAGAGCGGCCGC -3 For the S16AS127A mutein, the construct...

Ngày tải lên: 07/03/2014, 05:20

... 20 25 25 25 30 30 30 35 35 35 J Substrate + CF (IP) K Substrate + LF (IP) L Substrate + EF (IP) 5 10 10 10 15 15 15 20 20 25 25 30 30 35 35 Relative Fluorescence 20 22.91 25.92 25 30 35 Relative ... Journal 274 (2007) 31 38 31 49 ª 2007 The Authors Journal compilation ª 2007 FEBS 31 43 Determination of cathepsin E and D activity N Zaidi et al A TAPA Cytosol CatE activity CaD activity Lysosomes ... FEBS 31 45 Determination of cathepsin E and D activity A Undigested substrate N Zaidi et al B Substrate + CatE C Substrate + CatD 5 10 10 10 15 15 15 20 20 25 25 30 30 30 35 35 35 D Substrate...

Ngày tải lên: 07/03/2014, 09:20

... the C-terminal region is important for determining specificity Appl Environ Microbiol 62, 33 13 33 18 43 Fimland, G., Jack, R., Jung, G., Nes, I.F & Nissen-Meyer, J (1998) The bactericidal activity ... daily before each experiment using apomyoglobin [MH+ ¼ 16 952.56] and trypsinogen [MH+ ¼ 23 981.9] as standards for MBP fusion proteins and insulin [MH+ ¼ 5 734 .59] and insulin chain B [MH+ ¼ 34 96.96] ... activity and CD spectra of which were indistinguishable from the parent 66-amino acid peptide, preCbnB2 15N HSQC [29], 15N HSQC-TOCSY [30 ], 15N HSQC-NOESY [30 ], 13C HSQC, 13C HSQC-NOESY [31 ] and a...

Ngày tải lên: 07/03/2014, 15:20

... 60 80 100 Time (min) 20 40 60 80 100 Time (min) Fig (A) ATPase activity of EmbR (a) EmbR was incubated with [32 P]ATP[cP] for various time periods, and the release of 32 Phosphate (32 Pi) was monitored ... resulted in 73% and 79% dephospho- Autoradiogram Autoradiogram Phosphorylated forms of PknH and EmbR are substrates of Mstp in vitro PknH EmbR 80 60 40 20 0 10 + Mstp 30 30 − Mstp 30 (min) Heat ... MgCl2, mm dithiothreitol) with lg of EmbR and lCi of [32 P]ATP[cP] and incubated for 30 at 37 °C The reactions were stopped by the addition of SDS sample buffer, and proteins were separated by 1D gel...

Ngày tải lên: 16/03/2014, 14:20

... Therapy (8 13) -745-6866 Physical Activity Goal: 30 minutes of moderate physical activity or more days a week! • • • Physical activity in any form protects against certain types of cancer and against ... shows that increased physical activity reduces risk of endometrial cancer Every minute counts, so start with what you can now and work up to 30 minutes at a time Ten minutes done three times a day ... high-fat foods, and increasing physical activity can help lower weight and reduce body fatness Body Mass Index (BMI) • • • BMI is calculated from a person’s height and weight and can be a reliable...

Ngày tải lên: 22/03/2014, 11:20

... tuberculosis, pooled specificity for T-SPOT (52%; 95% CI, 40%– 63% ; studies [34 , 37 , 40, 41]) was similar to that for QFT-GIT (50%; 95% CI, 35 %–65%; studies [37 , 38 , 40, 41, 49]) (Figure 3) An insufficient number ... tuberculosis HSROC and/ or bivariate pooled sensitivity estimates were higher for T-SPOT (76%; 95% CI, 45%–92%; studies [34 , 37 , 40, 41]) than for QFT-GIT (60%; 95% CI, 34 %–82%; studies [37 , 38 , 40, 41, ... individuals, and 21(78%) studies included ambulatory patients (Table 1) IGRAs were performed for persons suspected of having active tuberculosis in 14 studies (52%) [34 , 36 38 , 40, 41, 46, 47, 49] and...

Ngày tải lên: 22/03/2014, 18:20

... CONTENT=" Video games and teenagers"> 2001-05-26 EXAMPLE OF FORMAL NOTATION This date has been written using the YYYY-MM-DD format, also known as W3CDTF (W3 Consortium Date and Time Formats) Thus, if ... schemes for other elements and their definitions are provided at: http://dublincore.org/usage/terms/dc/current-elements/ Metadata standards and subject indexing – Metadata standards for the web: ... for its authority for its elements and elements and qualifiers qualifiers Any organization can create their own namespace as long as they are committed to its maintenance Metadata standards and...

Ngày tải lên: 24/03/2014, 03:20

... Campbell J Physical activity and its determinants before and after college graduation Med Exercise Nutr Health 1994 ;3: 3 23 34 151 Cardinal BJ The stages of exercise scale and stages of exercise ... Promotion Centers for Disease Control and Prevention MS K -32 4770 Buford Highway, NE Atlanta, GA 30 341 -37 24 (888) CDC-4NRG National Heart, Lung, and Blood Institute Information Center PO Box 30 105 Bethesda, ... models and to plan and participate in family activities that include physical activity Parental support is a determinant of physical activity among children and adolescents ( 133 ,141,154,157 ), and...

Ngày tải lên: 28/03/2014, 09:20