... cofactor-binding domain, orRossman fold (residues 1–147 and 31 3 33 4 in spinachGAPDH (accession code in PDB:1JN0), the other being the catalytic domain (residues 148 31 3). The latter c omprises the S ... nvolved in transitory interactions [1 ,3, 14,21– 23] , but the residuesessential for these interactions remain unknown. Inthe past[24], we have shown that the conserved residue arginine 64of C. reinhardtii ... essential for the binding of CP12. The presence of this region at the interface of GAPDH andCP12 could also explain the kinetic changes o bserved for the binding of the substrate when the wild-type...
... could be used in place of the language shown in boldwithout changing the meaning of the sentence. Remember that you may need to change the formor in some cases the grammatical class of the word: ... night to finish their work, but it is impossibleto maintain this for very long and so it is not recommended.8. One of the main causes of the increase in inner-city lawlessness is the number ... controversy,…2. The murder was a very strange case but the most striking aspect… 3. All new cars on the market today have to conform… 4. The Pope in Rome is – according to the doctrine…5. Cars...
... provided their employees Internet access in the workplace. Forty-six of these companies were inthe service sector,whereas the remaining were inthe manufacturing sector. These companiesranged in ... between the two individuals, the level of trust will move back and forth based on the sum of all their interactions.Therefore, the resultant trust level at any point in time is the sum of the sequenced ... contain PWU. These are: (1) framing an ‘Internet use A Multidimensional Scaling Approach to Personal Web Usage inthe Workplace 79Copyright â 2004, Idea Group Inc. Copying or distributing in print...
... Plasma ProteinsHaving entered the blood, drugs maybind to the protein molecules that arepresent in abundance, resulting in the formation of drug-protein complexes.Protein binding involves primarily ... from binding sites onplasma proteins. Binding to plasma pro-tein is equivalent to a depot in prolong-ing the duration of the effect by retard-ing elimination, whereas the intensityof the effect ... area inthe sche-matic drawing, bottom left) and acts asa continuous barrier between the twospaces separated by the cell layer – in the case of the gut, the intestinal lumen(dark blue) and the...
... MTs inthe brain, and particularlywithin the injured or diseased brain. Inthe case ofGIF, the protein has many neuroprotective propertiesthat could provide vital protection during stress-inducedconditions. ... M(1991) The growth inhibitory factor that is deficient in the Alzheimers-disease brain is a 68-amino acid metallo-thionein-like protein. Neuron 7, 33 7 34 7.Cu-GIF+Reducing agentReducing agentZn-GIF++Aβ-Zni)Aββ-Cu(II)Aβ-Cu(I) ... led to intense interest inthe rolesof the entire MT family inthe brain, with particularfocus on the role of these proteins inthe injured orneurodegenerative brain.Discovery of GIFAD is...
... complex. The amino acid residues that determine the zymo-genicity of free FVIIa are as interesting as thoseinvolved inthe TF-induced allostery. Amino acid resi-dues in FVIIa that are involved inthe ... with the same affinity as the corresponding forms of FVIIa, and burial of the N-terminus of the protease domain increased similarly upon sTF binding toG372A-FVIIa and FVIIa. Thus Gly372(2 23) in ... located inthe vicinity of the active site and sensed by the peptidyl substrate are in uenced, in contrast to the remote exosites, e.g. in the vicinity of the activation pocket, that affect FXbinding,...
... to the a1subunit of PDH1, though it did contain one ADP-binding motif. This suggests the ADP-binding motif in the a1 subunit mediates the observed ATP binding,and FMN is likely situated at the ... two ADP-binding motifs [6]spanning residues 120–149 and 271 30 0 (Fig. 3) [3] . The a1 subunit of PDH1, by contrast, contained only oneADP binding motif spanning residues 108– 136 (Fig. 3) ,which ... that the ADP-binding motif in the b1 subunit mediates FAD binding. On the otherhand, FMN was not detected bound to either subunit(Fig. 5B,C). We also extracted the flavin compoundsfrom the native...
... the amount of enzyme that unwinds 30 % of the DNA helicase substrate at 37 °Cin30min(1 %in one min). For examining the effect of DNA-interactingcompounds on DNA unwinding activity of PDH120, the compounds ... February 20 03, accepted 20 February 20 03) Eur. J. Biochem. 270, 1 735 –1745 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 035 32.x In order to understand the mechanism of DNAunwinding we tested the ... inthe3 5Â directionand the others inthe 5 3 direction with respect to the strand on which they initially bind. Most organisms encodemultiple DNA helicases because of their involvement in numerous...
... suggesting that Gln3 83 might be involved in substrate binding and, possibly, specific recognition ofindole -3- pyruvate.Substrate recognition The indole moiety of the modelled intermediate is bound in a ... domains in ThDP-dependent enzymes.(Received 19 January 20 03, revised 28 March 20 03, accepted 2 April 20 03) Eur. J. Biochem. 270, 231 2– 232 1 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 036 01.x ... domainswith an open a/b class topology: the N-terminal PYR1domain (residues 3 180), which binds the pyrimidine part ofThDP; the middle domain (residues 181 34 0); and the C-terminal PP domain...
... aa). The structurallyhomologous residues in Mglu (33 4, 33 6 -33 8, 34 4, 419,420, 422 and 428 aa; nine aa in total) were locatednear the interface of the physiological dimer. The C-terminal domain ... differences in the interactions across the domain interface. All the resi-dues involved here seem to be unrelated to the Tris-binding site residues (including Arg2 23 and Ser227),either directly or indirectly. ... Similarly, the 33 orderedresidues inthe T structure (that are disordered in the N form) are not related to the Tris-binding site resi-dues in any way. Thus, the effect of Tris binding on the glutaminase...
... l-arginine into l-ornithine [16]. The lat-ter is in turn used by ODC to produce putrescine, the precursor of spermidine and spermine. To further ana-lyze the regulation of the polyamine-synthetic ... is involved in the inhibition of protein translation and cell prolifer-ation [33 ,34 ], it may participate in IRF -3- mediatedapoptosis. Another potential mechanism involves sper-mine, which induced ... of the tetracycline inducible cell line, rtTA-IRF -3 5D-Jurkat, with doxycycline for 36 h, in the presence of neutralizing antibodies against IFNs [14].ArgII mRNA was strongly induced in IRF-3...
... 15 May 20 03, revised 17 July 20 03, accepted 31 July 20 03) Eur. J. Biochem. 270, 38 29 38 38 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 037 77.x labelled UDP-glucose into glycogen inthe absence ... lMrapamycin prior to cell stimulationwith 100 nMinsulin for 30 min or 50 mMLi for 60 min. Inhibitors were present throughout the period of incubation with insulin and Li. At the endof these incubation ... IRS-2 and IRS -3 [2–5]. Of the numerous IRS binding proteins, the serine/lipid kinasephosphoinositide 3- kinase (PI3K) has been implicatedstrongly as a component of the signalling cascade thatstimulates...
... regularity. They depend upon the rains and the melting of the snows in their basins. The greatest average height is attained inthe late winter andearly spring months; another rise takes place inthe ... raised the light upperdecks. The Lexington, having destroyed the trestle-work at the end of the bridge, rejoined the following morning;and the three boats, continuing their raid, arrived the next ... feet. The forward plating was 3 inches of iron,backed by 30 inches of oak; at the stern, and abreast the engines, there was 2ẵ-inch iron, backed by 12 inchesof oak; the rest of the sides of the...