3 apos ss is not necessary for rev function

... 1494 ( -35 ) (-15) 265 ( -35 ) (-15) 265 ( -35 ) (-15) 265 ( -35 ) (-15) 265 60 160 45 35 30 25 40 475 620 236 5 1 533 33 05 85 245 65 265 240 520 255 180 290 105 95 545 170 510 458 133 5 190 38 0 85 535 155 ... 10 29 33 37 41 45 41a 1494 pol nef pol nef pol nef pol nef 239 ∆nef 239 ∆nef 239 ∆nef 239 ∆nef 055 wt 055 wt 239 wt 055 wt 239 wt 055 wt 239 wt 055 wt 239 ∆nef 239 ∆nef 239 wt 055 wt 239 wt 055 ... before [35 ] ELISPOT assay, ELISA, and flow cytometry The ELISPOT assay used for detection of IFN-γ secretion by CD8+ T cells was modified from Larsson et al [25] and previously described [35 , 43] ...

Ngày tải lên: 13/08/2014, 09:20

... 2009, 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 for the delivery of peptide nucleic acids (PNAs) to the nuclear compartment of living cells J Mol Biol 2002; 31 8: 237 -2 43 Pipkorn R, ... of apoptosis is not associated with the transformation process to malign cells [37 ] But affected cells show a prolonged cellular survival time and rate [38 ] compared with normal tissue Both events ... been identified [ 23, 24] which in their entirety can be responsible for the constricted sensitivity against alkylating agents It is clear that effort is necessary to search for new forms of treatment...

Ngày tải lên: 26/10/2012, 09:48

... A 30 .6 36 .0 33 .4 33 .3 2.7 8.1 B 18.6 24.4 23. 7 22.2 3. 2 14.2 C 11.7 44.6 33 .8 30 .0 16.8 55.8 D 29.8 23. 6 29.1 27.5 3. 4 12 .3 E 36 .4 30 .4 38 .2 35 .0 4.1 11.7 F 13. 5 15.4 18.1 15.7 2 .3 14.8 G 27 .3 ... 79. 03 2.04 2.58 0.762 71.21 75.22 71.71 69.85 72.00 2.29 3. 18 0.254 59.49 67.06 62.80 59. 43 62.20 3. 61 5.80 0.085 43. 23 57.00 49.56 47 .31 49.28 5.78 11. 73 0.028 31 .18 38 .57 36 .08 35 .06 35 .22 3. 07 ... 11.2 F 9 734 9 89816 882 13 91792.6 632 7.6 6.9 G 1 036 44 1141 53 114181 110659.2 8417.7 7.6 H 88285 96544 929 83 92604 5061.8 5.5 I 1 036 90 1011 93 74 133 930 05 .3 16 437 .9 17.7 J 105782 82502 60611 82964.7...

Ngày tải lên: 18/06/2014, 15:20

... predict the clinical efficacy of aspirin? Semin Thromb Hemost 2005, 31 :404-410 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Cannon CP, McLean DS: Critical pathways using platelet testing ... also formed distinct factors that were possible to rename according to measured property It is notable that serum TXB2 formed a distinct group not correlated to any of the other measurements It is ... 0.1 38 (17–59) 9.9 ± 1 .3* ** 29.4 ± 3. 6* 7.5 ± 1.4*** ADP 0.6 74 (60–89) 32 .5 ± 2.7*** 62.1 ± 3. 3** 22.9 ± 2.9*** SFLLRN 5 .3 76 (55–98) 28.8 ± 4 .3* ** 48.5 ± 5.2*** 20.2 ± 4.0*** Control (0.9 3. 1)...

Ngày tải lên: 18/06/2014, 15:20

... HUH82A BAQ30A BAG40A LT41A ISC18A ISC39A ISC76A ISC4A ISC24A ISC26A ISC36A ISC90A RT15A RTH33A RACT43A RACT44A RACT38A RT23A %+ Peripheral blood %+ Thymus 38 40 34 12 99 99 99 28 8 99 99 99 93 99 ... necropsy Cells from the respective tissues were isolated by mincing the tissues with a scissors and then passing the tissue preparation through a 100 mesh stainless steel sieve and suspended in PBS ... lymphocytes ISC4A, ISC24A, ISC26A, ISC36A, ISC90A, and RT15A (Table 1, Fig #31 , #26, #36 , #90 and #35 ) Two color analysis showed ISC4A and ISC24A recognize the same molecule (FC not shown) The others...

Ngày tải lên: 07/08/2014, 20:23

... Relapse First treatment Dosage of prednisolone mg/day SR 17 1/16 34 .4 ± 11.9 4±6 10 57.6 ± 6.4 SS 18 3/ 15 39 .7 ± 12.6 3 6 12 62.1 ± 3. 9 Normal 27 4/ 23 35.8 ± 13. 3 - - - - *Values are the mean ± standard ... 20 03, 24 :35 3 -35 8 Tissing WJ, Lauten M, Meijerink JP, den Boer ML, Koper JW, Sonneveld P, Pieters R: Expression of the glucocorticoid receptor and its isoforms in relation to glucocorticoid resistance ... cytokine and GR expression (P > 0.05, data not shown) Page of 11 (page number not for citation purposes) Discussion It is known that GC therapy is the treatment of choice for patients with SLE...

Ngày tải lên: 09/08/2014, 14:22

... purposes) Clinical and Molecular Allergy 2005, 3: 9 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 ity, specificity and monitoring specific immunotherapy Allergy 2004, 59:1102-9 Ebo DG, Hagendorens ... the basis of side scatter characteristics (SSC, y-axis) and CD203c (x-axis) Left histogram depicts resting cells, basophils express low levels of CD203c (some of them are not distinguishable ... after crosslinking of FcεRI with anti-IgE antibodies [28 ,30 ] This suggests that CD203c up-regulation is more or less specific to the crosslinking of FcεRI (figure 2) Page of (page number not for citation...

Ngày tải lên: 13/08/2014, 13:22

... wish to provide a framework that is easily adaptable and extendable to more complex assays and phenotypes Volume 7, Issue 8, Article R77 R77.12 Genome Biology 2006, 26 27 28 29 30 31 32 33 34 ... no effect, we use the Fisher test [ 23] Where x is the perturbation signal, y is the response, m is a smooth function (for example, a piece-wise polynomial), and ε is a noise term We obtain an ... response is essentially a yes/no decision, here the response is of a gradual nature (Figure 4b) (b) (a) Phenotype 800 11 Phenotype 600 64 1000 400 53 (b) 800 200 43 Well 0 delta=0.05 32 Well 200...

Ngày tải lên: 14/08/2014, 17:22

... velocity, ‘p’ is the pressure, ‘Si’ is a mass-distributed external force per unit mass, ‘e’ is the internal energy, ‘QH’ is a heat source or sink per unit volume, ‘τij’ is the viscous shear stress tensor, ... (6) This viscosity is a function of the turbulent kinetic energy ‘k’ and its dissipation rate ‘ε’ The equations of turbulent kinetic energy and its dissipation rate are written as: ISSN 2076-2895 ... Environment (IJEE), Volume 3, Issue 1, 2012, pp.61-72 67 The 3D propane mass fraction is presented in Figure for the two manifolds As shown in this figure, the mixture is basically lean in the...

Ngày tải lên: 05/09/2013, 16:30

... (1998) A review of practical techniques for the diagnosis of malaria Annals of Tropical Medicine and Parasitology Vol 92, pp 419 433 , ISSN: 00 03- 49 83 McHugh T.M (2005) Performance Characteristics ... 190, No 3, pp 1 036 1044, ISSN: 0021-91 93 Makler, M.T., Lee, L.G., & Recktenwald, D (1987) Thiazole orange: A new dye for Plasmodium species analysis Cytometry Vol 8, pp 568570, ISSN: 0196-47 63 Makler, ... Vol 6, page 130 , ISSN: 1471- 233 4 Lapinsky, S.E., Glencross, D., Car, N.G., Kallenbach, J.M., & Zwi, Set (1991) Quantification and Assessment of Viability of Pneumocystis carinii Organisms by Flow...

Ngày tải lên: 08/03/2014, 19:20

... Acquisition and Analysis 47 Table Correlated List Mode File: Values for Each Parameter Cell P1 P2 P3 Pn C1 C2 C3 C4 Cn 122 424 186 101 33 0 84 92 83 98 79 120 120 562 487 130 440 460 122 133 452 ... Tools for Single-Cell Analysis, Wiley-Liss, New York 22 Ormerod, M G., ed (2000) Flow Cytometry: A Practical Approach, 3rd edit Oxford University Press, Oxford 23 Darzynkiewicz, Z., Crissman, ... performance validation, data displays, and gating strategies 3. 1 Evaluating Instrument Performance Before acquiring data, it is most important to assess instrument performance To accomplish this,...

Ngày tải lên: 08/04/2014, 12:51

... of the experiment This is especially important for beginning flow cytometry users This discussion is typically not a critical review of the experiment but an excellent means for ensuring that appropriate ... overlayed for comparison Histograms are excellent tools for data analysis because they allow the user to visually see the distribution of a single measured parameter for the acquired events A histogram ... common display 1sa histogram A typical histogram data plot is shown in Fig This type of plot is probably the easiestto interpret and understand because information from a single parameter is displayed...

Ngày tải lên: 11/04/2014, 09:41

... My Mind, Motown Records 37 4 63- 031 4-2 (1972), Talking Book, Motown 37 4 63- 031 9-2 (1972), Innervisions, Motown 37 4 63- 032 6-2 (19 73) , and Fulfillingness’ First Finale, 37 4 630 332 -2 (1974) However, despite ... scholarly study of his life or his works—an enormous omission, given the high levels of popular, critical, and artistic success of his music Yet there is another, more compelling reason for analyzing ... Americana,” Reading Pop, 39 Note that Winkler is referring to the study of vocal styles, for which this is a particularly acute problem Indeed, in my preliminary work for this dissertation, I recognized...

Ngày tải lên: 29/05/2014, 21:49

... 0.027 CD31 ++++.+- CD29+CD31+CD56+CXCR4+CD90+Sca1- 0.027 CD31 ++.+-+-+ CD29+CD31+CXCR4+CD105-CD90+Sca1-CD44+ 0. 036 CD31 ++.+-+- CD29+CD31+CXCR4+CD105-CD90+Sca1- 0. 036 CD31 ++.+-.-+ CD29+CD31+CXCR4+CD105-Sca1-CD44+ ... Invest 2008, 118:294 -30 5 11 Gattinoni L, Powell DJ, Rosenberg SA, Restifo NP: Adoptive immunotherapy for cancer: building on success Nat Rev Immunol 2006, 6 :38 3 -39 3 12 Hassan HT, El-Sheemy M: ... comprehensive analysis of all 2 43 (35 ) possible phenotypes, as defined by 0, 1, 2, parameters, using a custom Java program as described in the Methods We utilize a shorthand notation for phenotypes by...

Ngày tải lên: 18/06/2014, 16:20

... (1998) A review of practical techniques for the diagnosis of malaria Annals of Tropical Medicine and Parasitology Vol 92, pp 419 433 , ISSN: 00 03- 49 83 McHugh T.M (2005) Performance Characteristics ... 190, No 3, pp 1 036 1044, ISSN: 0021-91 93 Makler, M.T., Lee, L.G., & Recktenwald, D (1987) Thiazole orange: A new dye for Plasmodium species analysis Cytometry Vol 8, pp 568570, ISSN: 0196-47 63 Makler, ... Vol 6, page 130 , ISSN: 1471- 233 4 Lapinsky, S.E., Glencross, D., Car, N.G., Kallenbach, J.M., & Zwi, Set (1991) Quantification and Assessment of Viability of Pneumocystis carinii Organisms by Flow...

Ngày tải lên: 28/06/2014, 15:20

... Luthra HS, et al.: The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis Arthritis Rheum 1988, 31 :31 5 -32 4 Girbal-Neuhauser E, Durieux JJ, Arnaud ... chemistry [ 13] The sensitivity of this assay was 63. 6% for pepA and 54.2 % for pepB at a specificity of 98.5% in established RA [14] With the development of the second generation anti-CCP2 ELISA, ... osteoarthritis (31 %), soft tissue mechanical complaints 20% (including peri-arthritis scapulohumeralis and tendinopathies), spondyloarthropathy ( 13% ), systemic lupus erythematosus (9%), vasculitis (6%),...

Ngày tải lên: 09/08/2014, 08:22

... 131 ± 35 137 ± 41 72 ± 66 ± 13 SvO2 (%) AVP/NE 71 ± AVP/NE* 0.75 ± 0 .31 0.51 ± 0.19 NE 0.65 ± 0. 23 0.44 ± 0.15 0.44 ± 0.16 NE 0 .31 ± 0.2 0.29 ± 0.19 7 .30 ± 0.12 7 .30 ± 0.12 NE 7 .33 ± 0.1 7 .32 ... (oscillations/min) 16.7 ± 7.7 15 .3 ± 10.2 70 ± 47.5 49.4 ± 24.8 15. 73 ± 5.15 14 .38 ± 7 .31 AVP/NE NE 7.49 ± 3. 2 0.548 14 .38 ± 4.85 13. 78 ± 9.17 8.99 ± 3. 92 0.089 0.56 0.055§ 10.7 ± 3. 81 AVP/NE‡ NE ODS reperfusion ... Baseline hour P 64 ± 81 ± 12 0.047† 3. 8 ± 1.1 3. 5 ± 0.9 3. 5 ± 0.8 AVP/NE 571 ± 140 526 ± 130 482 ± 111 4 93 ± 119 AVP/NE 155 ± 39 157 ± 35 NE VO2I (mL/min/m2) 66 ± 13 4.1 ± 1.2 NE DO2I (ml/min/m2)...

Ngày tải lên: 12/08/2014, 23:22

... test: a review J Investig Allergol Clin Immunol 2002, 12:1 43- 154 Page 13 of 14 (page number not for citation purposes) Clinical and Molecular Allergy 2008, 6:12 34 35 36 37 38 39 40 41 42 43 44 ... Na2-EDTA Figure of CD203c in CD63non expressing human basophils Expression Expression of CD203c in CD63non expressing human basophils Basophils were incubated for 30 minutes at 37 °C in the presence ... 2008, 146:177-189 21 22 23 24 25 26 27 28 29 30 31 32 33 Monneret G: Is the time for CRTH2/DP2 in a flow cytometric basophil activation test? Clin Exp All 2008, 38 :1 239 -1240 Hennersdorf F, Florian...

Ngày tải lên: 13/08/2014, 13:22

Ngày tải lên: 13/08/2014, 21:21

... λ is the mean free path of air, which is equal to 65nm The Brownian force is modelled as a white noise process [14] Accordingly, the amplitude is given as: FBrownian = ζ πS ∆t (3) where ζ is ... used in this paper The diameter of the pipe is considered 0.45cm in this paper [16] The resolution of the mesh is an important issue for simulating the particle deposition Figure displays the ... different analytical expressions for the fully developed flow in pipes based on the diffusion parameter Note that the point source analysis in a uniform flow is performed for validating the Brownian...

Ngày tải lên: 09/09/2015, 10:06