3 ¢ cdna library and 454 sequencing

... Proteins were visualized with CBB, cut out, and subjected to gas phase protein sequencing (model LF-3400 DT; Beckman) cDNA cloning, genomic PCR and sequencing A QuickPrepÒ Micro mRNA Purification ... hilaris larval midguts First-strand cDNA synthesis from the isolated mRNA and the following amplification of the target cDNA were performed with a SMARTTM RACE cDNA Amplification Kit (Clontech) ... primers designed from the cDNA sequence resulted in the amplification of bands in each case Sequencing of these bands showed that they matched the sequence of the cellulase cDNA completely; however,...

Ngày tải lên: 17/03/2014, 10:20

... Arabidopsis, times those of rice and poplar, and times that of sorghum Full-length enriched cDNA library construction To construct a full-length enriched cDNA library containing highly variable ... clones, the average lengths of the 5'- and 3'-untranslated regions (UTRs) were 127 bp and 203 bp, respectively, and the longest 5 'and 3' -UTRs were 486 bp and 480 bp, respectively Functional annotation ... bands However, 26 primer sets (59%) produced smears or multiple bands that were not countable and only 18 primer pairs (41%) amplified clear separate bands (Additional file 5) The smeared bands...

Ngày tải lên: 12/08/2014, 03:21

... GB15931-PA and NH homology on reverse strand * • GB10007-PA on reverse strand *** • Near ab initio prediction and NH homology on reverse strand ** Ab initio prediction on reverse strand *** Near ... that is, derived from at least two independent cDNA clones (Table 1) Quality of the cDNA clones and sequences Results and discussion Generation and assembly of fire ant ESTs To survey the fire ... the fire ant we produced a cDNA microarray using all 22,560 clones sequenced from the cDNA library We successfully PCR-amplified 17,685 (78.4%) cDNAs (only one strong band, Additional data file...

Ngày tải lên: 14/08/2014, 17:22

... PCR products of cDNAs cloned randomly from the full-length cDNA library Lanes 1-16, cDNA fragments amplified from randomly selected positive clones with the vector-specific T3 and T7 primers by ... cDNA library, and the inserted cDNA fragments were confirmed by PCR amplification, which revealed that most of the cDNA inserts ranged from 0.4 to 2.5 kb (Fig 4) These data showed that the cDNA library ... primary cDNA library was 3.6 × 10 cfu -1 10 mL and that of the amplified library was 1.2 × 10 cfu -1 mL , with a recombination rate of 99% Sixteen positive clones were randomly picked from the cDNA...

Ngày tải lên: 11/11/2016, 15:59

... MS and SS and constructed full-length cDNA library and did preliminary sequencing and analysis All authors read and approved the manuscript Acknowledgements: We are grateful to José Polonia and ... soil treatments and under both drought-stress and irrigated conditions and that whole plants (above and below ground parts) were harvested at seven and three timepoints for the cDNA preparations, ... full-length cDNA library here and the libraries from Ramírez et al [12] for leaf tissue and for that of Thibivilliers et al [14] This shows the high degree of normalization of the full-length cDNA library...

Ngày tải lên: 11/08/2014, 11:21

... gratitude and sincere thanks to Professor Dr Takashi Hosoda who suggested me this research topic, and provided guidance, constant and kind advices, encouragement throughout the research, and above ... the following published and/ or accepted journal and conference papers: Anh T N and Hosoda T.: Depth-Averaged model of open channel flows over an arbitrary 3D surface and its applications to analysis ... and Vreugdenhill, C B 1973 Calculations of two-dimensional horizontal flow Rep S163, Part 1, Delft Hydraulics Laboratory, Delft, The Netherlands Steffler M P and Jin Y C 1993 Depth averaged and...

Ngày tải lên: 06/11/2012, 10:35

... ………you (do)…………… this Sunday? - I (visit) my grandparents 10.I think Liz and her parents (go) ……………………to Tri Nguyen Aquarium in Nha Trang III Choose and underline the best answers: Her mother (left/ ... places: The children love Disney land They want to go ……………… again I open the box and look………………… She ran…………………… to open the door for him Let’s go……………………… and sit in the sun Wait………………… I’ll ... ……………………………………………………………………………………………… Snow is falling very heavily and the wind is blowing hard (be a snow storm) → There … ………………………………………………………………………………………… The traffic lights are red The woman and her son are at the zebra crossing...

Ngày tải lên: 09/07/2013, 01:26

... TO MENU GETTING STARTED Look at the pictures and talk about the activities in the countryside BACK TO MENU GETTING STARTED Look at the pictures and talk about the activities in the countryside ... TO MENU GETTING STARTED Look at the pictures and talk about the activities in the countryside BACK TO MENU GETTING STARTED Look at the pictures and talk about the activities in the countryside ... TO MENU GETTING STARTED Look at the pictures and talk about the activities in the countryside BACK TO MENU GETTING STARTED Look at the pictures and talk about the activities in the countryside...

Ngày tải lên: 29/09/2013, 21:10

... appears You can have some script execute when you show and/ or hide the pop up by using the onpopupshowing and onpopuphiding methods, as when you show and hide items in a dynamic menu The second step ... items like menus and buttons 3.4 Tabular and Hierarchical Information Many options exist to display hierarchical information in your user interface The most common are tree-like and table-like ... boxes, trees, and grids With the exception of the tree, these elements are not limited in regard to the content they can contain Currently, the tree only holds text and image content and grids are...

Ngày tải lên: 17/10/2013, 19:15

... activities: its interaction with its users and other applications, its responsibility for maintaining business rules and integrity, and its responsibility for recording and storing a chronology of events ... systems Command and Memento probably would not be chosen because they mostly handle user interaction scenarios Iterator could be chosen for any of these use cases for retrieving and working with ... identify common patterns for interaction with users and other systems, for maintaining business rules, and for storing data Discovering useful patterns and using them in the logical design of objects...

Ngày tải lên: 19/10/2013, 02:15

... distributed random numbers with mean and variance 1: xn ˆ 2*sqrt(3)*(rand À 0:5); For two random variables x and y, we have E‰x ‡ yŠ ˆ E‰xŠ ‡ E‰yŠ, …3:3:15† i.e., the mean value of the sum of random ... results between (a) and (b) (d) A ˆ 1, f ˆ 600 Hz, and fs ˆ kHz (e) Compare and explain the results (b) and (d) 14 Generate 1024 samples of pseudo-random numbers with mean and variance using the ... random variable from a uniformly distributed random variable using N ! 12 The Gaussian random variable is frequently used in communication theory The MATLAB function randn generates pseudo-random...

Ngày tải lên: 19/10/2013, 23:15

... when the view is expanded and collapsed setCellText (row, colID, value) Called when the contents of the cell have been edited performAction (action) An event from a set of commands can be invoked ... to be defined and placed in a set In Example 3-12, the first column holds the labels and the second contains the text boxes These two columns are horizontal to each other and in rows ... Text Display Three tags available in XUL handle basic text display in the UI, and each has its own context for use They include a , a , and a element The caption is...

Ngày tải lên: 20/10/2013, 09:15

... ... has a command set that has its own commands and that pulls in a second set defined elsewhere (moreEditItems)

Ngày tải lên: 20/10/2013, 09:15

... aRandomNumber + " equals " + (someNumber+aRandomNumber)); %> And here's the output 97 plus equals 973 97 plus equals 100 [output] You Say Seven, I Say 14 The JSP Files (part 3): Black Light And ... " + aRandomNumber + " equals " + (someString+aRandomNumber)); %> And here's the output 97 plus equals 100 97 plus equals 973 You Say Seven, I Say 15 The JSP Files (part 3): Black Light And White ... (part 3): Black Light And White Rabbits And the output is: The string black light starts with bl The string white rabbit ends with it Screaming Out Loud 13 You Say Seven, I Say And finally, in case...

Ngày tải lên: 23/10/2013, 00:15

... splitter changes when a user handles the and collapses the splitter, for example, and then quits the persist attribute preserves the splitter state and its visibility for the next ... and These two elements create space between widgets in a box and can be horizontal or vertical depending on the orientation of the box The separator is a visible divider and the ... flicker and UI wobbles when content is being shown and hidden intermittently 3.9.3.2 Overflow A value of scroll or auto for the CSS overflow property ensures that a scrollbar appears and that...

Ngày tải lên: 24/10/2013, 08:15

... Print, and Open Notes [1] Chapter has more information on RDF datasources To delve deeper into the chrome layout and install issues, see Chapter 3.12 The Extras Certain lesser-known elements and ... simple, and the locator is href 3.13 Building the Application Shell Now that the main XUL widgets and some crucial concepts like the box model have been described, you can bring things together and ... abbreviated help and the display of values that are otherwise obscured in the UI In the Mozilla application, the most common places where they are used are on toolbar buttons and splitter grippies...

Ngày tải lên: 24/10/2013, 08:15

... current list of business objects, services, and attributes that may not have been explicitly listed in the usage scenario Activity 5.3: Identifying Attributes and Relationships 35 Exercise 2: Identifying ... logical model of one aspect of the solution indicating the business objects, their attributes and services, and the relationships among the business objects After completing the above steps, you will ... and Relationships 35 Exercise 2: Identifying Business Object Relationships (20 min) ! Identify candidate relationships Participate in small groups as assigned by the instructor Review the future-state...

Ngày tải lên: 10/12/2013, 16:16

... activities: its interaction with its users and other applications, its responsibility for maintaining business rules and integrity, and its responsibility for recording and storing a chronology of events ... systems Command and Memento probably would not be chosen because they mostly handle user interaction scenarios Iterator could be chosen for any of these use cases for retrieving and working with ... identify common patterns for interaction with users and other systems, for maintaining business rules, and for storing data Discovering useful patterns and using them in the logical design of objects...

Ngày tải lên: 10/12/2013, 16:16

... steps are completed, logoff, by typing exit, and turn all the devices off Then remove and store the cables and adapter 4-4 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.6 Copyright ... command Switch_A#show spanning-tree brief b On Switch_B type show spanning-tree brief at the privileged EXEC mode prompt as follows: Switch_B#show spanning-tree brief Examine the command output and ... brief 3-4 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.6 Copyright  2003, Cisco Systems, Inc c What changes have taken place in the command output? On Switch_A? ...

Ngày tải lên: 11/12/2013, 14:15