3 pretreatment of milk using separation techniques

... composition of dairy products 12.3 Pretreatment of milk using separation techniques 12.4 Standardization and concentration of milk proteins in the dairy industry 12.5 Isolation of whole ... 1.5 Cascade of extractors for extraction of solid materials One of the major advantages of SFE processes is the fractionation of extracts Multi-step separation may be performed by use of several ... energy costs of the process One of main advantages of using SCF for extraction and fractionation of substances, is that by a stepwise reduction of solvent power, fractionation of extracts can...

Ngày tải lên: 20/09/2015, 01:57

... accompanied by the release of cytochrome c from mitochondria, oligonucleosomal degradation of genomic DNA, activation of apoptotic caspases, and proteolytic cleavage of caspase substrates Although ... the functions of endogenous BIR family proteins by immunodepletion with specific antibodies The efficiency of depletion could not be accurately estimated because of the low contents of endogenous ... was not possible because of the low concentrations of the endogenous molecules As an alternative approach, each of four MBP fusions was added at lgÆmL)1 at the start of incubation, and the remaining...

Ngày tải lên: 30/03/2014, 16:20

... optimal temperature of 65 ◦ C and activity of 900 AGU/g protein An amyloglucosidase unit, AGU, is the amount of enzyme able to hydrolyze ␮mol of maltose per minute at 37 ◦ C and a pH of 4.3 Ethanol ... optimal pH 5.5, optimal temperature of 85 ◦ C and activity of 120 KNU-S/g enzyme A kilo novo unit, KNU-S, is the amount of enzyme that breaks down 5.26 g of starch per hour Glucoamylase cleaves ... extraction and hydrolysis processes Assessment of statistical significance was made at an ˛ value of 0.05 2.4 Extraction of anthocyanins and subsequent hydrolysis of ISP residues ISP roots were sliced...

Ngày tải lên: 05/05/2014, 08:39

... suspended in 0.2 ml of TM buffer Expression and purification of proteins VP51 and VP76 are the products of ORF wsv308 and wsv220 of WSSV (GeneBank accession no AF332093) and composed of 466 and 674 ... on immunofluorescence flow cytometry to study the localization of VP51 and VP76 by comparing the http://www.virologyj.com/content/3/1/76 mean fluorescence intensity of the population of intact ... better understanding of the molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection in the future Conclusion The localization of the two proteins,...

Ngày tải lên: 20/06/2014, 01:20

... synthesis of precursor proteins of the inner layer subunits under the influence of estrogen, at least in most species [16–19,51,66] Despite the confusing terminology used to designate this very Page of ... J, Yasumasu I and Yamagami K Cloning of cDNAs for the precursor protein of a low-molecular-weight subunit of the inner layer of the egg envelope (chorion) of the fish Oryzias-latipes Dev Biol ... proteins of the inner layer of the envelope that surrounds the oocyte of the ovulated teleost egg We have used zona radiata proteins, a descriptive term, to designate these proteins because of the...

Ngày tải lên: 13/08/2014, 13:20

... first round of screening Half of the colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting ... Discussion 3.1 Principle of Split-Ubiquitin Screening System Studies have shown that the use of yeast two hybrid system, which is a genetic assay for in vivo detection of interactions of proteins, enables ... located at the C terminus of Cub will be cleaved off and result in downstream reactions, which is the activation of LacZ and HIS3 reporter genes [7] 3.2 Identification of Cellular Membrane Proteins...

Ngày tải lên: 02/11/2012, 11:08

... ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide corresponded to that of ... the elution profiles The profile of steaming-extracted polymer (A) contained two saccharide peaks (at elution volumes of 70 ml and 90 ml) and one UV-absorbing peak (at elution volume of 95 ml) The ... washing-extracted polymers contained large amounts of phosphorus (of 3.2% and 3.6%, respectively) Table shows molar ratios of the constituent neutral sugars of the polymers Although the molar ratio varied...

Ngày tải lên: 05/09/2013, 09:08

... 104 Activity 12.1: Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct an origami boat using the sheet distributed by the instructor ... completing the above steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS PAGE INTENTIONALLY LEFT BLANK ...

Ngày tải lên: 24/01/2014, 10:20

... contact cut-off To evaluate the influence of different cut-off values in our study, replicate experiments were conducted ˚ ˚ ˚ using A, 10 A and 12 A distance cut-offs In nearly all of our tests, ... successful examples of binding-sequence discrimination, the native sequences of the RRM1 of Sex-lethal protein (1B7F_1) and KH1 domain of Poly C-binding protein-2 were both ranked first out of 256 sequences, ... (cut-off ¼ A) for RRM ⁄ KH domain proteins The red line represents the rank of cognate recognition sequences using the contactcounting score; the blue line represents the rank of these sequences using...

Ngày tải lên: 18/02/2014, 16:20

... 0.8 0.6 0.4 0.2 min Fig Time-lapse LSM analysis of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in ... suggesting the existence of some interactive cellular partner FRAP analysis of the nuage revealed the slow recovery of OlvasGFP (Fig 3B) When the whole part of the single compartment of nuage was bleached, ... formation of the granules is not merely an articial phenomenon, but that it could be used for investigation of the features of PGCs Results Time-lapse laser scanning microscopy (LSM) image analysis of...

Ngày tải lên: 18/02/2014, 16:20

... 2549 Study of the interactions of the Anp32a LRR module C de Chiara et al A B Fig Solution structure of the LRR domain of murine Anp32a (A) NMR bundle of the 10 best structures in terms of energy ... the aim of mapping the surface of interaction between the two proteins This method, which relies on the effect that binding of a molecule has on the electron distribution of another, causing a ... different regions of the molecule As part of a long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ disease proteins, we report here a study of the structural...

Ngày tải lên: 18/02/2014, 17:20

... C-terminal sequence of about 90 amino acids of unknown function The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% identity) argues for a conservation of the tertiary structure ... normalize the expression data of Cg-Clp1 and Cg-Clp2 transcripts The relative level of expression of each target gene was calculated for 100 copies of GAPDH transcript by using the following formula: ... Fig Multiple sequence alignment of Cg-Clp2 with members of the GH18 family (A) The predicted amino acid sequence of Cg-Clp2 is aligned with the amino acid sequence of three CLPs from the oyster...

Ngày tải lên: 19/02/2014, 00:20

... and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of anti-PrP antibodies ... nonglycosylated isoform (m), was analyzed using densitometry The percentages of each band regarding to the total signal of PrPC were calculated as arithmetic means of separate gel runs The number of gel ... di-glycosylated isoform of PrPC than did antibodies to the structured core region However, the glycoform patterns of mouse PrPC always showed the highest signal intensity of the di-glycosylated isoform,...

Ngày tải lên: 19/02/2014, 02:20

... 103Q), a broad distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent ... percentage of the cell population with an FL2-H fluorescence greater than · 101 was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the percentage of ... scraped off the culture dish in NaCl ⁄ Pi Following centrifugation, pelleted cells were resuspended in mL of water An equivalent of mg of total cell protein was used per assay After addition of 500...

Ngày tải lên: 19/02/2014, 06:20

... attempted a similar experiment to seek stable mutants of barnase Using a subset of codons that only encode hydrophobic residues, a library of barnase mutants was made by randomizing the residues ... Selection of folded proteins by phage-display (Eur J Biochem 271) 1611 Fig Effect of structural change on the selection (A) Structure of cyt b562 Residues M7 and H102 are the ligands of heme Heme ... bundle protein in the absence of any cofactors In this work, the authors used the method similar to that of Finucane et al [11] except that the protein A B-domain instead of His-tag was used to select...

Ngày tải lên: 19/02/2014, 12:20

... viscosity of the extract as a result of DNA, lL of DNase I (200 units) was added per 100 lL of buffer [30] Following filtration through two layers of miracloth and the addition of four volumes of ice-cold ... ÔStainsAllÕ analysis of Fig and the 1D immunoblotting of Fig As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of a-dystroglycan ... with antibodies to the Dp427 isoform of dystrophin (A), the a-subunit of the dystroglycan complex (a-DG; B), the Na+/K+ ATPase (NKA, C), the fast SERCA1 isoform of the sarcoplasmic reticulum Ca2+...

Ngày tải lên: 19/02/2014, 16:20

... study the effect of different parameter settings There are several parameters in the framework: the number of generated seeds r, the number of new candidates k2 and the number of selections k ... classification, instead of using all sentences In this experiment, we only use sentiment related words as features to represent opinion documents for classification, instead of using all words Our ... and Ryan McDonald 2008 A joint model of text and aspect ratings for sentiment summarization In Proceedings of the 46th Annual Meeting of the Association of Computational Linguistics: Human Language...

Ngày tải lên: 19/02/2014, 19:20

... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... (and increase recall) we next performed a simple transformation of the derived rule set If all children of a rule tree node are of type *scope* or * (i.e noncue words), the node label is replaced ... the rule tree; neighboring identical siblings of type *scope* or * are replaced by a single node of the corresponding type Figure shows an example of this transformation The rule tree pruning...

Ngày tải lên: 20/02/2014, 04:20

... context vector Vm of a morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair of the surface string and the part -of- speech of mi The ... 2003; Nakano and Hirai, 2004) formalized the task of extracting NEs as a chunking problem of a sequence of characters instead of a sequence of morphemes In this paper, we keep the naive formalization, ... SF , and the ones of “Baseline” show the results without SF The column of “NExT” shows the result of using NExT(Masui et http://mecab.sourceforge.net/ http://chasen.org/∼taku/software/CRF++/ http://chasen.org/∼taku/software/...

Ngày tải lên: 20/02/2014, 09:20

... angles of most of the residues of the Bowman±Birk inhibitor are distributed fairly evenly over the b- and PPII-regions of the Fig A MOLSCRIPT diagram [67] of the X-ray crystal structure of soybean ... beam, which prevented the acquisition of ROA data of suf®cient quality for reliable analysis A ROA spectrum of rather poor quality of an impure commercial sample of a-casein (composition unde®ned) ... examples of protein aggregation However, although the presence of signi®cant amounts of PPII structure may be necessary for the formation of regular ®brils, other factors must be important as, of all...

Ngày tải lên: 21/02/2014, 15:20