3 stimulation of endothelial cell monolayer formation

... from the cell surface and circulate as a soluble form of the receptor Circulating levels are indirect measures of the degree of endothelial activation (b) Hemostasis Activated endothelial cells ... circulating levels of endothelial biomarkers are only indirect measures of endothelial cell activation, and thus may not accurately reflect the degree, nature and site of activation of the intact ... The aim of the present study was to assay a broad range of endothelial markers in a large sample of human patients at the time of emergency department (ED) presentation with the goal of gaining...

Ngày tải lên: 13/08/2014, 21:21

... phospholipase A2 is in the nucleus of subconfluent endothelial cells but confined to the cytoplasm of confluent endothelial cells and redistributes to the nuclear envelope and cell junctions upon histamine ... or BDH (Poole, Dorset, UK) Cell culture The EA.hy.926 cell line, a hybrid of human umbilical vein endothelial cells (HUVEC) and A549 human lung carcinoma epithelial cells [21], was a generous ... presence of lM calcium, most of the cPLA2-a was membrane-bound The cytosolic location of lactate dehydrogenase confirmed that cells were lysed sufficiently Quantification of the relative levels of cPLA2-a...

Ngày tải lên: 30/03/2014, 13:20

... that the endothelial cell plays a crucial role in angiogenesis [39] and that it might affect the growth and differentiation of neighboring cells [40] The endothelial cell is also the target cell ... on endothelial cell proliferation, migration and invasion [12] has sparked an interest in identifying cellular models of angiogenesis as well as molecular mechanisms amenable to endothelial cell ... migration and tubular formation of human endothelial cells in vitro Am J Physiol 40, E433–E438 Spencer, C.A & Groudine, M (1991) Control of c-myc regulation in normal and neoplastic cells Adv Cancer...

Ngày tải lên: 21/02/2014, 01:21

... processes leads to endothelial dysfunction and damage Exposure of endothelial cells to pro-inflammatory mediators results in an up-regulation of E- and P-selectin, vascular cell adhesion molecule-1 ... in granules of endothelial cells and platelets and it translocates to the cell surface following exposure to inflammatory stimuli E-selectin is exclusively expressed by endothelial cells, and L-selectin ... changes, transcriptional induction and redistribution from intracellular pools [20] Integrins mediate cell cell, cell extracellular matrix and cell pathogen interactions by binding to distinct, but overlapping,...

Ngày tải lên: 07/03/2014, 12:20

... location of the nuclei in these cells These results confirm that the cells isolated by our procedure are endothelial cells from the vein of human umbilical cord Flow cytometry of endothelial cells ... types of cells, such as epithelial cells (airways, alimentary tract, urogenital tract, epidermis), mesothelial cells (pleura, pericardium), immune cells (human leucocytes), muscle cells, endothelial ... (unstimulated) of this molecule on endothelial cells Induction of E-selectin (N and C) expression over endothelial cells is observed after stimulation with IL-1b (A–E) The expression of wVf over...

Ngày tải lên: 16/03/2014, 23:20

... volume of some solid tumours [90– 92] One of the main targets of these treatments comprises the endothelial cells One endothelial cell can control the survival of approximately 50–100 tumour cells ... cells [93] Therefore, the destruction of a few endothelial cells may induce the death of a large number of tumour cells Moreover, it was shown that endothelial cell suppression could also mediate ... observed after the repetition of cycles of of hypoxia followed by of reoxygenation for weeks [101] Moreover, in vitro, an increase in endothelial cell migration and formation of tubes was also reported...

Ngày tải lên: 30/03/2014, 04:20

... receiving the impact of the fetal milieu The UC is an exceptional source of vascular cells, which can offer valuable information about the cellular characteristics of the blood vessels of the individual ... http://www.translational-medicine.com/content/7/1/30 age of cells expressing Ki67 (see below) from the total number of cells Cellular markers The expression of von Willebrand (vW) factor, CD31 (platelet endothelial cell adhesion molecule-1, ... distribution of endothelial cell projection area Average4 Average percentage distribution of endothelial cell projection area The average percentage distribution of the projection area of human umbilical...

Ngày tải lên: 18/06/2014, 15:20

... include the translation of the input RNA, processing of the polyprotein, formation of membranous replication complexes, uridylylation of the terminal protein VPg, synthesis of minus and plus strand ... separation of 5S protomers and 14S pentamers The amount of radioactivity at the bottom of the tubes of the gradients was not determined (A) Comparison of samples obtained in the absence or presence of ... Cells and viruses HeLa R19 cell monolayers and suspension cultures of HeLa S3 cells were maintained in DMEM supplemented with 5% fetal bovine calf serum Poliovirus was amplified on HeLa R19 cells...

Ngày tải lên: 19/06/2014, 08:20

... development of dyskinesia Transplantation of stem cell derived dopamine-producing cells offers a number of potential advantages over fetal transplants, including the ability of stem cells to migrate ... strategy Potential sources of stem cells include endogenous liver stem cells (such as oval cells), ES cells, bone marrow cells, and umbilical cord blood cells Although a series of studies in humans ... gastrointestinal precursor cells may be candidates for cell- based therapy of diabetes Nervous System Neural cells have been differentiated from a variety of stem cell populations Human ES cells can be induced...

Ngày tải lên: 07/07/2014, 01:20

... role of PAF antagonists in the rescue of apoptosis is being explored in our laboratory We have undertaken to determine the source of PAF in these cultures FDCs have features of cells of myeloid -cell ... al Follicular dendritic cells, interdigitating cells, and cells of the monocyte-macrophage lineage are the C1q-producing sources in the spleen Identification of specific cell types by in situ 56 ... uptake of [ H]thymidine than either of the two cell types alone (see Figure 1, upper panel) Addition of the PAF antagonist WEB 2170 (10Ϫ6 to 10Ϫ8 M) to mixed B lymphocytes consistently diminished cell...

Ngày tải lên: 08/08/2014, 20:23

... using bovine serum albumin as the standard Cells Endothelial cells Human umbilical vein endothelial cells (HUVECs) were obtained from the Endothelial Cell Facility UMCG (Groningen, The Netherlands) ... inflammation-associated cellular events They affect the endothelial cells per se by inducing the expression of a complex array of genes, thereby changing the endothelial activation status and the balance between cell ... responses of dnIκB expressing HUVECs to TNF-α activation The larger the number of viral particles /cell (× 103) of AddnIκBPEG-RGD used for the infection, the higher the percentage of ICAM-1dim cells...

Ngày tải lên: 09/08/2014, 07:20

... added to each 100 uL of cell suspension containing x 106 cells (otherwise µL of antibody per x 106 cells) and incubated on ice for 30 Two milliliters of Fluorescence Activated Cell Sorting (FACS) ... will lead to loss of limb and life A particular vascular event that occurs in diabetes is increased expression of cellular adhesion molecules on the surface of vascular endothelial cells VCAM-1 is ... markers, which in turn are recognized by cellular adhesion molecules that are located on the surface of the endothelial cells of the intima Activated immune cells translocate into the vascular tissue...

Ngày tải lên: 11/08/2014, 03:20

... PHIS-01 Induction of anti-tumor effector T cells Since induction of cytolytic effector cells is pivotal in ensuring the success of tumor vaccines, we investigated relative efficacy of phytols and ... culture plates at × 106 cells/well in ml RPMI/10% FBS, and then stimulated in vitro with killed E.G7-Ova cells or 2C3 cells (1.2 × 106 cells/well) for days in the presence of 10% CO2 at 37°C to ... phytols are hydrophobic, they are capable of interacting with the cell membrane A number of recent studies have described various cellular and biological effects of phytol (19–21) However, there is...

Ngày tải lên: 11/08/2014, 10:23

Ngày tải lên: 11/08/2014, 10:23

... cytoplasm of infected cells (Fig 1A) This is the first report to our knowledge of PIC infection of human endothelial cells Effects of PIC infection on HMEC-1 permeability To investigate the effect of ... primary source of TNF-α in PIC infection We have demonstrated PIC infection of human endothelial cells; and that PIC infection of HMEC-1s induces the production of NO Analysis of the iNOS gene ... HMEC-1 cells are susceptible to PIC infection PIC infection induces the production of NO NO was determined to be an important factor in the loss of endothelial cell monolayer integrity Inhibition of...

Ngày tải lên: 12/08/2014, 04:20

... [10] In view of the importance of endothelial function in cardiovascular homeostasis, the mechanisms underlying endothelial activation and the development of endothelial dysfunction are of great ... exposed cultured endothelial cells to Page of 12 (page number not for citation purposes) Discussion A basic role for exosomes in intercellular communication implies that the cell of origin controls ... inhibition of diacylglycerol kinase-α (DGK-α) in T lymphocytes increased the secretion of proapoptotic exosomes [37] Inhibition of DGK isoforms allows full activation of the diacylglycerol/Ras/extracellular...

Ngày tải lên: 13/08/2014, 08:20

... the formation of sieve plates The study of the luminal cell membrane of freeze-fractured LSEC revealed the presence of trabecular meshworks which were attached to the E and P-faces of the cell ... series of rings of fenestrae-associated cytoskeleton (middle) Layering a colored scanning electron micrograph on top of the cell surface of a nonextracted rat liver sinusoidal endothelial cell ... the cell membrane of both the cell body and the attenuated cell processes Trabecular meshworks are a cell membraneattached reticulum of anastomosing trabeculae composed of the cell membrane and...

Ngày tải lên: 13/08/2014, 13:20

... accumulation of triglyceride (TG) as one of the hallmarks of adipogenesis The anti-obesity effect therefore could be represented by the suppression of TG formation in 3T3-L1 adipocytes Preparation of herbal ... terms of their abilities to stimulate ApoA-IV expression and TG formation Cell culture The stable cell line of Caco-2/TC7 transfected with the human ApoA-IV promoter was provided by Prof M Lacasa ... the specificity of amplification was confirmed by melting curve Preparation of lipid micelles The lipid micelles was used to mimic the duodenal micelles resulting from digestion of lipid [30],...

Ngày tải lên: 13/08/2014, 15:21