3 cell sources growth and differentiation

... Food and Drug Administration These polymers are favorable for the synthesis and secretion of a cartilaginous matrix, such as proteoglycans and R557 type II collagen, and act as a physical and ... decalcified in EDTA, and embedded in paraffin Sagittal sections (5 µm thick) were prepared from the center of the defect area and stained with hematoxylin and eosin, alcian blue, and safraninO–fast ... C, 38.8 ± 16.9% in group T-4, and 76.5 ± 18.7% in group T-12 (Fig 3) Significant differences in the %MM were seen between C and T-12 (P = 0.007) and between T-4 and T-12 (P = 0.007) The average...

Ngày tải lên: 09/08/2014, 06:22

... temperature (23±10) and humidity (60-80%) controlled room under regular light and dark conditions All procedures were reviewed and approved by the Institutional Animal Care and Use Committee at ... Schenk R, Buser D Bone healing and graft resorption of autograft, anorganic bovine bone and beta-tricalcium phosphate A histologic and histomorphometric study in the mandibles of minipigs Clin Oral ... preparation and histomorphometric analysis The rats were euthanized and tibiae were excised Samples were fixated in 10 % buffered formaldehyde solution Soft tissues were cleaned and the specimens...

Ngày tải lên: 25/10/2012, 11:15

... growth factors such as nerve growth factor, insulin-like growth factor, fibroblast growth factor, epidermal growth factor (EGF), and hepatocyte growth factor can suppress apoptosis of target cell populations; ... transport and drug metabolism, mutations of the p53 tumor suppressor gene, and overexpression of oncogenes [9,10] In addition, several studies indicate that growth factors such as nerve growth factor, ... understood [11–16] Growth factors are multifunctional cytokines involved in many biological processes including proliferation, differentiation, migration, and cell survival They bind and activate a...

Ngày tải lên: 19/02/2014, 06:20

... diffusion coefficients of gold and indium on silicon, the solubility of substrate atoms in the two metals, the surface tension of gold and indium and the surface energy of silicon and silicon oxide Fig ... found From the phase diagrams In–Si and Au–Si (Fig 4), it can be seen that the solubilities of silicon in gold and indium at our growth temperatures are 420 and o1 mol%, respectively It could ... wire growth Conclusions The different behavior of gold and indium on Si(1 1) has been described and analyzed An explanation based on differences of solubilities of surface atoms in gold and indium...

Ngày tải lên: 16/03/2014, 15:17

... Surgery and Research 2008, 3:36 The limb (and thus the)virtual cylinder is composed of soft tissue (mainly muscles and skin) and "hard" tissue (bone and bone marrow) The soft tissue volume (VS) and ... right and left upper limbs, and 265 ± 22 and 256 ± 20 ml for the right and left lower limbs, respectively At steady state blood flow measurements in the four limbs ranged between 5.5 – 6.5 and ... measured between right and left limbs, both in the upper and the lower extremities Discussion Normal growth, remodeling and repair of bone require delivery of nutrients and oxygen through blood...

Ngày tải lên: 20/06/2014, 01:20

... anti-CD68 (a and d) and anti-Flt-1 (b and e) Abs, followed by TRITC- and FITC-conjugated secondary Abs, respectively The images were merged (c and f) Arrows indicate monocyte-like cells (a-c) and arrowheads ... with anti-CD68 (Figure 2a and 2d) and anti-Flt-1 (Figure 2b and 2e) Abs, followed by TRITC- or FITC-labeled secondary Abs, respectively As shown in Figure 2c and 2f, CD68 and Flt-1 co-localized ... cells, pOCs and OCs Then, through paracrine and autocrine mechanisms, the Page of Figure Expression of pY-Flt-1 and pY-FAK in GCTs The sections were prepared as described in Fig and stained with...

Ngày tải lên: 20/06/2014, 04:20

... anti-CD68 (a and d) and anti-Flt-1 (b and e) Abs, followed by TRITC- and FITC-conjugated secondary Abs, respectively The images were merged (c and f) Arrows indicate monocyte-like cells (a-c) and arrowheads ... with anti-CD68 (Figure 2a and 2d) and anti-Flt-1 (Figure 2b and 2e) Abs, followed by TRITC- or FITC-labeled secondary Abs, respectively As shown in Figure 2c and 2f, CD68 and Flt-1 co-localized ... cells, pOCs and OCs Then, through paracrine and autocrine mechanisms, the Page of Figure Expression of pY-Flt-1 and pY-FAK in GCTs The sections were prepared as described in Fig and stained with...

Ngày tải lên: 20/06/2014, 07:20

... conceived of the study and participated in its design and coordination, acquisition of data, analysis and interpretation of data, and was fully involved in drafting the manuscript and revising it critically ... III-12 and III-13) with increased circulating levels of AP (more than 120 units/l); presumably affected by PDB (II-3 (Fig 2a), II-8 and II-10); and potentially mutant (II-1, II-2, II-7 and III-5; ... subjects (22 females and 15 males; age range 33 to 92 years) and 18 deceased individuals (11 males and females; Fig 1) Members from generations I to III were farmers born and still living in a...

Ngày tải lên: 09/08/2014, 07:20

... independently that BMP-4 and BMP-5 expression is related to the synovial lining layer in ND and more to the sublining layer in RA and OA patients (Figures and 5) Figure Expression of BMP-4 and BMP-5 in synovial ... A similar distribution and predominant expression of different BMPs in fibroblastoid and macrophagocytic cells was also shown by Lories and colleagues [18] and van Lent and colleagues [26] That ... recruitment and performed the 'synovitis score' AP and CK conducted part of the patient recruitment and data evaluation FS, GAM and GRB provided substantial input into data evaluation All authors read and...

Ngày tải lên: 09/08/2014, 07:20

... treatment and 8-fold increase for TNF-α treatment relative to vehicle), dermal (14fold and 4-fold increase) and synovial (31-fold and 7-fold) fibroblasts (Figure 5) By contrast, classical T-helper-1 and ... supervised collection of tissue and generation of cells, and co-wrote MH developed initial hypothesis, supervised all cell analyses and co-wrote All authors read and approved the final manuscript ... cells and macrophages However, it was only weakly expressed by dendritic cells The enzyme was abundantly expressed by CD90-positive fibroblasts and by endothelial cells (identified by von Willebrand...

Ngày tải lên: 09/08/2014, 08:22

... Obs, Syp phosphorylation and activity could compensate for this reduction As Syp is central to other growth factors, such as HGF and epidermal growth factor [43,44], and since we recently showed ... (n = 5) and OA (n = 5) Obs IGF-1R levels under basal conditions The phosphorylation of IGF-1R following IGF-1 stimulation was very rapid and similar in normal (n = 3) and OA (n = 3) Obs and increased ... 2.86 ± 0.36 and 2.52 ± 0.06-fold, respectively, above basal levels after 30 s (Figure 3b), and phosphorylated Figure Results Phenotypic characteristics of normal and OA osteoblasts and response...

Ngày tải lên: 09/08/2014, 08:23

... biphosphonates At baseline, serum PTH and 25-OHD levels were 30.5 ± 17.6 pg/ml and 19.7 ± 10.3 ng/ml, respectively Serum osteocalcin and serum CTX-I were 19.9 ± 11.8 ng/ml and 446 ± 307 pg/ml, respectively ... turnover, the osteocalcin and CTX-I levels remained the same at baseline and after year The same stability was observed for 25-OHD and PTH levels In the infliximab group, femoral and lumbar BMD values ... responders and nonresponders The change in lumbar spine BMD was +0.4% (0.890 ± 0.163 g/cm2 at baseline and 0.894 ± 0.154 g/cm2 year later) for nonresponders and -0.8% (0.955 ± 0.141 g/cm2 at baseline and...

Ngày tải lên: 09/08/2014, 10:20

... mass and bone remodelling [32-34] and have been demonstrated to this by controlling both osteoblast and osteoclast differentiation and function [65-67] WNT signalling, in terms of bone mass and ... insulin-like growth factor-2 and TGF-β1 in cortical bone from the iliac crest [38]; matrix metalloproteinase (MMP)2 and liver alkaline phosphatase in subchondral bone [4]; and IL-1β, IL-6 and TGF-β1 ... expression of WNT and TGF-β/BMP signalling pathway and target genes in OA bone The genes include those with known or suspected roles in osteoblast, osteocyte and osteoclast differentiation and function,...

Ngày tải lên: 09/08/2014, 10:21

... growth factor-I and serum IGF-binding protein can be associated with the progression of breast cancer, and predict the risk of recurrence and the probability of survival in African-American and ... MMTV-Cre mice and wild type C57Bl/6 mice were euthanized at 3, and 12 months of age to collect trunk blood For histology, mammary tissue from 3, and 12 month old mice was removed post mortem and formalin ... September 2011 References Hadsell DL, Bonnette SG: IGF and insulin action in the mammary gland: lessons from transgenic and knockout models J Mammary Gland Biol Neoplasia 2000, 5:19-30 Rinaldi S, Toniolo...

Ngày tải lên: 11/08/2014, 00:23

Ngày tải lên: 13/08/2014, 01:20

... and assembled the data MM, YT and KO provided study materials and critical revisions and important intellectual content NM contributed to the concept and design, provided critical revisions and ... concept and design, interpreted and analyzed the data, provided drafting of the article, provided critical revisions and important intellectual content, collected and assembled the data HK, JU, TO and ... of Stat3 and Stat5 by Jak kinases was investigated with Jak selective inhibitor, AG490 AG490 reduced constitutive phosphorylation in Stat3 [MT-2, HUT-102 and ED-40515(-)] and Stat5 (MT2 and HUT-102)...

Ngày tải lên: 13/08/2014, 09:21

... JO and JAR carried out the determination of MPM-9 and TIMP-1 and made substantial contributions to the analysis and interpretation of data JMBL and YB carried out the determination of TNF-α and ... APACHE-II scores were 19 and 24 in surviving and nonsurviving patients, respectively; and in the study by Hoffmann et al [11], the mean APACHE-II scores were 14 and 23 in surviving and nonsurviving patients, ... sepsis patients and 50 controls in terms of age and sex Higher serum levels of MMP10 (P < 0.001) and TIMP-1 (P < 0.001), and nonsignificantly Table Comparison of MMP-9, MMP-10, and TIMP-1 serum...

Ngày tải lên: 13/08/2014, 19:20

... variable, and aquarium, growth, and aquarium × growth as fixed effects Growth was estimated 100 Lingcod plasma IGF1 standard 90 80 B / Bo 70 60 50 40 30 20 10 0.01 0.1 10 100 1000 Peptide (ng IGF1 standard) ... Juan Island, NSh = North Shaw Island, and SSh = South Shaw Island PRIMER 6; Anderson 2001) to determine whether lingcod differed across space based on measurements of size, condition, and growth ... IGF1 and growth (n = 11, adjusted r2 = 0.077, P = 0.209) While the slopes and strength of the relationships between IGF1 and growth were qualitatively different in tank A versus tanks B and C,...

Ngày tải lên: 04/09/2015, 17:15

... PLLA nanofibers and (c) mineralized PLLA/Col nanofibers Peaks at 1755 cm-1 and 1086 cm-1 referred to carbonyl and C-O stretch in (a) PLLA Peaks at 1086, 1033 and 558 cm-1 in (b) and (c) referred ... FGF: Fibroblast Growth Factor FTIR: Fourier Transform Infrared Spectroscopy FDA: Food and Drug Administration GFD: Growth Differentiation Factor HA: Hydroxyapatite H&E: Hematoxylin and Eosin HFP: ... During trauma and disease conditions, loss of tissue may occur and instead of being in homeostasis state, the stem cells migrate out of the niche and start their proliferative and differentiation...

Ngày tải lên: 11/09/2015, 09:57