2non genomic effects of ar in pca cells
báo cáo hóa học:" Molecular analysis of the apoptotic effects of BPA in acute myeloid leukemia cells" potx
... inducing BAD phosphorylation in NB4 cells Finally, also non genomic actions such as the early decrease of both ERK and AKT phosphorylation were induced by BPA thus indicating that a complex intersection ... the endocrine interference This is also demonstrated by the fact that NB4 cells not display detectable levels of ER alpha Thus suggesting that effects of BPA in this cells are largely ER independent ... activation in NB4 cells BPA induces apoptosis via caspase activation in NB4 cells Caspase 8, and 37 assays have been carried out by FACS analysis in NB4 cells after 48 h of incubation with the indicated...
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Báo cáo khoa học: "Effects of propranolol in combination with radiation on apoptosis and survival of gastric cancer cells in vitro" ppsx
... activity in cancer cells, thus making the cells radioresistant [12] Activation of NF-B by various stimuli, including inflammation, stress and radiation, involves degradation of the inhibitory ... http://www.ro-journal.com/content/5/1/98 Page of Figure Expression of b-ARs in human gastric cell lines SGC-7901 and BGC-823 by RT-PCR and western blotting (A) Expression of bARs in human gastric cell lines SGC-7901 and ... Both of cell lines expressed b1- and b2 -AR mRNA (contol group had no cDNA) (B) Expression of b-ARs in human gastric cell lines SGC-7901 and BGC-823 at the protein level by western blotting Both of...
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Báo cáo y học: "Chemokine receptor expression and functional effects of chemokines on B cells: implication in the pathogenesis of rheumatoid arthritis" ppt
... [1,2], B cells are considered to play important roles in the pathogenesis of RA In this regard, the synovial tissue of RA patients shows abundant accumulation of inflammatory cells, including T cells, ... four chemokines induced migration of both CD27- and CD27+ B cells (Figure 3a) However, the migration induced by CCL21 was most prominent Comparison of the migratory effects of the chemokines on ... for 30 minutes 3H-thymidine was added and B cells were incubated for 24 hours The incorporated radioactivity was quantified Fold increase in 3H-thymidine incorporation in response to chemokine stimulation...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... Alizarin Red staining, respectively (original magnification × 10) The inset in the lower right panel represents an enlargement of the adipocyte indicated by the arrow origin dose-dependently inhibited ... upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation ... Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, were...
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Báo cáo y học: "Pleiotropic effects of statins in distal human pulmonary artery smooth muscle cells" doc
... of smooth muscle cells in vascular lesions [9,17] The addition of simvastatin to sildenafil also reversed hypoxia-induced pulmonary hypertension and remodelling[16] In keeping with findings in ... reductase inhibitors in experimental models of PH [20,21], raising questions about whether statins as a class of drugs are capable of inducing similar responses in the pulmonary vasculature of humans ... expression of RhoA in vascular smooth muscle cells [32] and the beneficial effects of sildenafil and simvastatin in hypoxia-induced PH depend, at least in part, on the inhibition of RhoA- and Rho-kinase-dependent...
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Báo cáo y học: "Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice" pps
... Results Effects of bone marrow-derived cells on monocrotalineinduced pulmonary hypertension (PH) PH developed within 15 days of MCTp injection, as shown by a sustained increase in right ventricular ... and paraffin embedding, µm-thick lung sections were cut and stained with hematoxylin-phloxine-saffron In each mouse, 30 intraacinar vessels accompanying alveolar ducts or alveoli were examined ... process, irrespective of the cause of PH, we also examined the effects of BMDC injection in mice with developing hypoxia-induced PH Interestingly, BMDC injection did not improve PH in this model Moreover,...
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ANTI CANCER EFFECTS OF THYMOQUINONE IN BREAST CANCER CELLS INVOLVEMENT OF NON HOMOLOGOUS END JOINING AND TELOMERE TELOMERASE HOMEOSTASIS
... mechanism of action of TQ in breast cancer cells In addition, it is of interest to investigate the effects of TQ in normal breast epithelial cells and thereby establish if the effects of TQ are indeed ... Therefore, it is of particular interest to investigate if TQ also affects telomeres and DNA integrity in breast cancer cells Most of the earlier reports investigated the effects of TQ in relation ... aprotinin, pH 7.2) to obtain whole cell lysates containing total cellular proteins Protein concentration was determined by bicinchoninic acid assay with bovine serum albumin as the standard (Pierce...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... transcript variants (TV) of exon of the hCDC4 gene Arrows mark the position of the detected mutation in the 5’ UTR of Exon 1-TV3 and the new intronic SNP downstream of exon 10 (B) Chromatograph of the ... sequencing in 35 samples derived from bone marrow or peripheral blood of AML patients at the time point of initial diagnosis This resulted in the identification of one heterozygous mutation in the ... were sequenced at the DLMBC sequencing service (Dr M Meixner) in the Department of Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems,...
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Tài liệu Gulf War and Health: Volume 4. Health Effects of Serving in the Gulf War docx
... Academy of Engineering The Institute of Medicine was established in 1970 by the National Academy of Sciences to secure the services of eminent members of appropriate professions in the examination of ... written-up in detail and typically are not included in tables COMPLEXITIES IN RESOLVING GULF WAR AND HEALTH ISSUES Investigations of the health effects of past wars have often focused on narrowly defined ... path of the sarin–cyclosarin plume Initially, however, because of the complexity of the modeling that needed to be done, CIA-DOD estimated that any noticeable effects of sarin and cyclosarin would...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... improving specific imaging and effective targeting of intracellular antigens by means of intrabodies Results Expression of destabilized scFvs in COS-1 cells The functional characteristics of the ... fused to d1-EGFP are maintained in the cells by binding to antigen Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular colocalization ... level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7 J Immunol Methods 231, 207–222 Rajpal A & Turi TG (2001) Intracellular stability of...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... POU homeodomain family proteins bind to this consensus motif and regulate gene transcription in diverse cells through interaction with a variety of partner DNA binding proteins, including Sp1 family ... matrix/adhesion protein, possibly necessary for development of a normal fetus and in regulating immune responses within the intestine Induction of fgl2 in macrophages in response to viruses results in the ... described in Fig of increasing amounts of Sp3 along with 50 ng of Sp1 (halfmaximum amount) demonstrated increased activity of fgl2 promoter compared to Sp1 or Sp3 alone (Fig 9A) In contrast, increasing...
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International Accounting Standard 21 The Effects of Changes in Foreign Exchange Rates potx
... carrying amount of inventories is the lower of cost and net realisable value in accordance with IAS Inventories Similarly, in accordance with IAS 36 Impairment of Assets, the carrying amount of ... currency are translated in accordance with paragraphs 38–50 19 This Standard also permits a stand-alone entity preparing financial statements or an entity preparing separate financial statements in ... delivery of a non-monetary asset EC staff consolidated version as of 18 February 2011 FOR INFORMATION PURPOSES ONLY Summary of the approach required by this Standard 17 In preparing financial...
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Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx
... receptors can interact directly with structural proteins such as b-actin, a-actinin, laminin a3 and integrin b2 in HEK cells, some of which might initiate cytoskeletal rearrangements following receptor ... differentiation of neuroblastoma cells Results Molecular characterization of purinergic P2X receptors in N2a cells In order to investigate the presence of native purinergic receptors in N2a cells, the ... receptors in N2a cells The pharmacological characterization of P2X receptors was carried out by analysing the change in intracellular free calcium concentration ([Ca2+]i) elicited by a variety of purinergic...
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Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx
... the binding of HB-19 Similarly, the iron-binding hTf had no effect On the other hand, bLf inhibited the binding of HB-19 to cells In accordance with the involvement of both lobes of hLf in the ... crosslinking of a ligand leads to the clustering or capping of its surface receptor Accordingly, we investigated the distribution of surface nucleolin following the crosslinking of bound hLf using ... lactoferrin (hLf) and HB-19 for binding to Chinese hamster ovary (CHO) wild-type and mutant cell lines (A) Inhibition, by HB-19, of hLf binding to CHO cells CHO cells were incubated (45 min,...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... validation in primary cells and animal models but, as a preliminary step, they unravel one of the mechanisms of TNFamediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells ... that, in the presence of TNFa, wherein cells are rendered insulin insensitive, insulin-mediated nuclear exclusion and inactivation of Foxa2 are prevented, with the result that it is primarily ... prevents insulin-induced inhibition of hepatic glucose output, indicating that, in such conditions, cells become insulin insensitive; this is in agreement with studies in which the overexpression of...
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Báo cáo Y học: Mydj2 as a potent partner of hsc70 in mammalian cells doc
... were used in order to determine the subcellular localization of the farnesylated and the nonfarnesylated forms of Mydj2 protein by Western blotting analysis, using the same anti-dj2 Ig as in immunofluorence ... the molecular chaperone DnaJ is involved in binding to denatured protein substrates EMBO J 15, 408–417 13 Karzai, A.W & McMacken, R (1996) A bipartite signaling mechanism involved in DnaJ-mediated ... Wyttenbach, A., Carmichael, J., Swartz, J., Furlong, R.A., Narain, Y., Rankin, J & Rubinsztein, D.C (2000) Effects of heat shock, heat shock protein 40 (HDJ-2) and proteosome inhibition on protein aggregation...
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industry and the effects of climate in italy
... concentration of volcanic phenomena, in part still active) as well as in the Alps Finally, the flat areas, including the great Po-Venetian Plain, are basically formed of mixed deposits that are mainly ... During the course of the succeeding Mesozoic era, lasting for over 160 million years, almost all the present area of Italy remained covered by a large marine basin on whose bottom (which varied ... the Arno and Tiber, flowing through central Italy into the Tyrrhenian The other main tributaries of the Po are the Ticino, Adda and Oglio, arising in the Alps, the Tanaro, from the Apennines,...
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Báo cáo khoa học: Anti- and pro-oxidant effects of quercetin in copper-induced low density lipoprotein oxidation Quercetin as an effective antioxidant against pro-oxidant effects of urate potx
... of the linear part of the kinetics of diene formation shown in Fig 2(B,D) with x-axis Half times for carotenoid consumption were obtained from the kinetic of carotenoid consumption shown in Fig ... of carotenoids was measured as an index of the overall consumption of the endogenous antioxidants of LDL during their Cu2+-induced oxidation As shown in Fig 3(A,C), carotenoids are consumed in ... either after a h incubation at 37 °C or at various times during incubation Consumption of carotenoids The basal carotenoid content of LDL preparations was spectrophotometrically determined after extraction...
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Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt
... al sugars in a similar way as proteins [5–7] The exocyclic amino group of 2¢-deoxyguanosine is particularly prone to glycation reactions, leading to the formation of N2-carboxyethyl, N2-carboxymethyl, ... we investigated whether CEdG accumulates in resting HSC-T6 cells After 14 days of incubation, a 1.7-fold increase of CEdG content in the genomic DNA of the cells was measured These results indicated ... end-products in cells 300 Glycation rate (%) 250 200 150 100 50 0 days of incubation 14 days of incubation Fig Increase in the glycation rate of genomic DNA from HSC-T6 cells, which were incubated...
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