2different ways to activate nf kappa b in hematological and solid malignancies

Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps

Ngày tải lên : 12/08/2014, 15:21

... was markedly inhibited not only by p38 MAPK inhibitor SB203580, but also by another p38 MAPK inhibitor SB202190, and NF- http://respiratory-research.com/content/9/1/16 kappa B inhibitor PDTC Moreover, ... MAPK blockade had any effect on Group B streptococci (GBS)-induced NFkappa B binding activity [21] Vallejo et al demonstrated that p38 inhibitor SB202190 inhibited GBS-induced activator protein ... DNA binding activity of NF- kappa B induced by NTHi A549 cells were incubated with different concentrations of NTHi with or without SB203580, and DNA binding activity of NF- kappa B was measured by...

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báo cáo hóa học: " Inhibition of NF-κB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia" docx

Ngày tải lên : 19/06/2014, 22:20

... sequestered in the cytoplasm by inhibitory proteins known as NF- B inhibitors (IκBs) Upon stimulation, I B is phosphorylated by an upstream I B kinase (IKK), which leads to its ubiquitination and proteosomal ... 5-LOX inhibition protects against IR injury in rats via down-regulation of the http://www.jneuroinflammation.com/content/3/1/12 inflammatory mediators NF- B and iNOS Thus, inhibiting the 5-LOX /NF- B ... ROS in lymphoid cells [9] However, the mechanisms of NF- B activation by direct interaction with 5-LOX remain to be investigated The 5-LOX inhibitor BW -B 70C used in this study inhibited NF- κB...

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Báo cáo y học: "κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocyte" ppt

Ngày tải lên : 09/08/2014, 01:23

... rheumatoid arthritis produce osteoclastogenesis inhibitory factor/osteoprotegerin: reciprocal regulation of the production by inflammatory cytokines and basic fibroblast growth factor J Bone Miner ... 0.1% Triton X-100/1 × PBS, followed by three washings Nonspecific binding was abolished by incubation for hour at room temperature in blockade solution (1 × PBS, 0.1% bovine serum albumin) Polyclonal ... three proinflammatory cytokines Even more interesting is the fact that some factors not activated by individual cytokine treatment were stimulated with their combination Fra-1 and NF- B were particularly...

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Báo cáo y học: "The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis" potx

Ngày tải lên : 09/08/2014, 07:20

... monocytes and in vitro-differentiated macrophage to produce proinflammatory cytokines such as TNF-α S100A8/A9 stimulation is reduced significantly by p38 MAPK inhibitors and NF- B inhibitors and induces ... MAPK inhibitors did not reduce the DNA-binding activity of NF- B, these two critical signaling pathways may be independently involved in S100A8/A9-mediated cytokine production NF- B inhibitors ... NF- B protein binding was verified by inhibition and supershift experiments with unlabeled NF- B consensus or mutant oligonucleotides and anti -NF- B p65 antibody (b) Effects of p38 MAPK inhibition...

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Báo cáo y học: "Elevated expression of CD30 in adult T-cell leukemia cell lines: possible role in constitutive NF-κB activation" pps

Ngày tải lên : 13/08/2014, 09:21

... containing NF- B binding sites (reviewed in [27]) Alternatively, NF- B activation occurs by inducible processing of NFKB2/p100 with I B- like inhibitory activity, into p52 with DNA binding activity, ... vector were cloned and the sequences were determined Following three independent screenings, we obtained a total of 64 clones (Table 1) NF- B inducing kinase (NIK) is a mitogen-activated protein ... SC: NF- kappaB-inducing kinase regulates the processing of NF- kappaB2 p100 Mol Cell 2001, 7:401-409 Xiao G, Sun SC: Negative regulation of the nuclear factor kappa B- inducing kinase by a cis-acting...

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Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

Ngày tải lên : 18/02/2014, 13:20

... p38 and nuclear factor -kappa B (NF- jB) in oridonin-induced apoptosis and autophagy were demonstrated in that they inhibited 1292 apoptosis but promoted autophagy Oridonin-induced p38 mitogen-activated ... oridonin-induced Beclin activation was inhibited by p65 siRNA, indicating that NF- jB promoted oridonin-induced L929 cell autophagy Oridonin treatment led to a decreased inhibitor kappa B (I-jB) ... survival and decreasing the sensitivity of cancer cells to apoptosis [21] Typically, in most unstimulated cells, NF- jB is sequestered in the cytoplasm by binding to the inhibitor of NF- jB (I-jB) In...

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

Ngày tải lên : 19/02/2014, 00:20

... (5) the NF- jB to bind to DNA The NF- jB subunits were characterized by using a speciﬁc antibody for the p50 or p65 subunit, and the results indicate that the NF- jB band intensity reduced in the ... cytokines to trigger febrile responses in rabbits Cytokines stimulated by NF- jB can also directly activate the NF- jB mechanisms and establish a positive autoregulatory loop to amplify the in ammatory ... NF- jB binding ability is abrogated by binding site competition and antibody supershift analysis These ﬁndings indicate that the stimulatory effect of SEC1 appears to require NF- jB For the febrile...

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Báo cáo y học: "DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lpr mice in vivo" potx

Ngày tải lên : 09/08/2014, 14:21

... Class AM14 B cells, and primary mouse (INH-ODNs) inhibit Toll-like receptor-7 backbone-dependent manner (DCs),R and B inhibitory oligonucleotides B cells in a sequence-independent but (TLR7)-dependent ... remains to be determined, several possibilities may be considered: (a) competition between TLR9inhibiting ODNs and endogenous DNA for binding to PL2-3 antibodies/nucleosome/HMGB1 complex, (b) ... in inhibitory activity between class B and class R INH-ODNs in resting (follicular) B cells was clearly dependent on the ability of INH-ODNs to make double-stranded secondary structures The biggest...

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báo cáo khoa học: "HGF/c-Met related activation of b-catenin in hepatoblastoma" pps

Ngày tải lên : 10/08/2014, 10:21

... Immunohistochemical staining of HB using an antibody to b- catenin (a) Cytoplasmic staining of b- catenin in hepatoblastoma (b) Nuclear and cytoplasmic accumulation of b- catenin in hepatoblastoma (c) ... Hepatoblastoma negative for staining with an antibody to Y654- b- catenin (b) Diffuse cytoplasmic staining of Y654- b- catenin (c) Nuclear and cytoplasmic staining of Y654- b- catenin in hepatoblastoma ... Histologic type and subtype, b- catenin and Y654 b- catenin IHC and CTNNB1 gene status of hepatoblastomas with mutations Case Number Histologic Type Histologic Subtype b- catenin Y654 -b- catenin...

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Báo cáo y học: "Early Inhibition of NF-κB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol)" pot

Ngày tải lên : 11/08/2014, 08:21

... μg/mL LPS and incubated at 37°C for 60 minutes After incubation the number of viable cells was determined by counting living cells after staining with trypane blue Determination of free p65 in nuclear ... tea, coffee, cocoa, wine and beer) blood samples were drawn to obtain basal values Subsequently, the volunteers took tablets containing 200 mg standardized maritime pine bark extract (Pycnogenol®, ... manufacturer's protocol Determination of NF- B activation by ELISA Plasma samples obtained before and after Pycnogenol intake were diluted 1:1 with RPMI medium as described above and incubated with...

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Báo cáo y học: "Dysregulated apoptosis and NFκB expression in COPD subjects" pptx

Ngày tải lên : 12/08/2014, 14:20

... Rel family proteins, p65 (RelA) and p50 (NF B) subunits In unstimulated cells NF B is sequestered in the cytoplasm due to its binding to I B and I B Following cell stimulation, I B is rapidly ... An ongoing bacterial infection may exacerbate airway inflammation in COPD subjects by signalling via Toll-like receptor (TLR) activation to activate NF B and induce expression of NF B- regulated ... possibly due to the low number of non-smoking controls available and the inter-subject variability To fully understand the involvement of NF B in neutrophil apoptosis in COPD, it is important to...

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Báo cáo y học: "Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis" pptx

Ngày tải lên : 12/08/2014, 17:22

... has been reported that many chemokines and proinflammatory cytokines induce the infiltration of inflammatory cells (mainly mononuclear cells and T cells) into the synovium of inflamed joints and ... subunits are involved in CXCL10-induced RANKL expression, we used PTX, a bacterial toxin that inhibits Gai activation by ADP-ribosylating Gai subunits, and the inhibition of Gai by PTX was confirmed ... heterotrimeric G proteins consisting of a, b, and g subunits The G protein resides attached to the Figure Induction of receptor activator of nuclear factor kappa- B ligand (RANKL) by CXCL10 in CD4+ T cells...

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Environmental and Social Impacts of Shrimp farming in Tam Giang lagoon, Vietnam-Local perception

Ngày tải lên : 02/11/2012, 10:15

... presentations and hosting me in Norway I am thankful to Mr Nguyen Luong Hien, Mr Nguyen Quang Vinh Binh, Ms Vo Tuyet Hong, Mr Tran Xuan Binh and Ms Lam Thi Thu Suu for providing me valuable information and ... An and Vinh Ha have been converted into shrimp farms Fishers and shrimp farmers tend to be more concerned about the impacts of shrimp farming on the nursery beds and breeding grounds compared to ... preparation and for buffering pH To kill red-blooded animals like fish, predators in shrimp ponds Increase primary productivity and increase turbidity Sterilize pathogens in input water and disease...

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B-to-B Marketing in 2009:Trends in Strategies and Spending

Ngày tải lên : 10/08/2013, 16:17

... testing not available due to variations in sample sizes for each tactic 28 B -to- B Marketing in 2009: Trends in Strategies and Spending ATTITUDINAL AND BEHAVIORAL ANALYSIS 29 B -to- B Marketing in ... Tactics and Budget Allocations 14 Attitudinal and Behavioral Analysis 29 B -to- B Marketing in 2009: Trends in Strategies and Spending RESPONDENT PROFILE B -to- B Marketing in 2009: Trends in Strategies ... 95% level of confidence: A /B 11 B -to- B Marketing in 2009: Trends in Strategies and Spending Customer Focus The main target market focus for most respondents is in the business -to- business arena,...

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ĐỀ THI KHẢO SÁT ĐẠI HỌC MÔN TOÁN KHỐI (A,B,D) - TRUNG TÂM LUYỆN THI _NGUYỄN TRUNG TRỰC

Ngày tải lên : 05/09/2013, 16:34

... CÂU VI : Cho tam giác cân ABC B có tung độ B khác -3.đỉnh A (-3,-3) đường tròn nội tiếp tam giác ABC có phương trình (x-1)2 + y2 =9 Tìm B CÂU VII: Cho ba điểm A(-1,-2,0) B( 3,1,2) C(1,0,1) mặt phẳng ... phẳng P cho b n điểm A ,B, C,D đồng phẳng Và b n điểm hình thang CÂU IX: Tìm số phức z biết z(1-2i) =(3+4i)(2-i)2 B I LÀM THÊM tinh tích fân sau : : d(x) ∫ (x31+1) 3 √1+x CHÚC CÁC EM LÀM B I TỐT ...

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Tài liệu ĐỀ THI THỬ ĐH-CĐ 2010 MÔN TOÁN KHỐI A–B–D–V_TRUNG TÂM THÀNH ĐẠT(ĐỀ 02) pptx

Ngày tải lên : 23/12/2013, 08:17

... VDPQCNB = VMCNB MC MN MB 6 ã Vỡ D l trung im ca MC nờn d ( M ,(CNB)) = 2d (D ,(CNB)) ị VMCNB = 2VDCNB = VDCSB = VS ABCD ị VDPQCNB = VSABNPQ 7 VS ABCD ị VSABNPQ = VS ABCD ị = 12 12 VDPQCNB Cõu V: ... Ax + By + Cz = (vi A2 + B + C ) ã Vỡ (P) ^ (Q) nờn: A + 1 .B + 1.C = C = - A - B (1) A + 2B - C ã d ( M ,( P )) = 2 A +B +C = ( A + B - C )2 = 2( A2 + B2 + C ) (2) B = (3) (4) ã T (3): B = ... sin t Tớnh c: B = 2p -2 ã Tớnh B = x - x dx = A + B ũ ã Tớnh A = = -2 Cõu IV: Gi P = MN ầ SD, Q = BM ầ AD ị P l trng tõm DSCM, Q l trung im ca MB ã VMDPQ VMCNB = MD MP MQ 1 = = ị VDPQCNB...

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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

Ngày tải lên : 18/02/2014, 11:20

... that is able to inhibit the second activation step of MMP-2 [9] The small calcium-binding protein S100A4 has been shown to regulate expression of MMPs and their inhibitors in several cell lines [10] ... (4–12%) and proteins were transferred to a poly(vinylidene diﬂuoride) membrane by electroblotting After blocking nonspeciﬁc binding sites with non-fat milk (5% solution), blots were incubated for ... catalytic domain Two major types of endogenous inhibitors regulate the activity of MMPs; a2-macroglobulin and four tissue inhibitors of metalloproteinases, TIMP-1 to TIMP-4 [2] In order to identify...

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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

Ngày tải lên : 22/02/2014, 07:20

... correspond to independent recombinant strains obtained from a cross involving CS9 and IJ2 parental strains ability of GBSSI to extend amylopectin outer chains Again this coincides with a decrease in ... 6.5 kb fragment in cosmid GB911 covering most of the GBSSI coding sequences was subcloned in two overlapping parts of 3.0 and 4.5 kb and subjected to DNA sequencing thus generating a 5856 bp gDNA ... methods to extract starch granulebound proteins are fully described in [18]) Before blotting proteins onto nitrocellulose membrane (Protean BAÒ, Schleicher & Schuell), the gels were incubated for...

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