2 the dairy industry and composition of dairy products

... Treatment of effluents and technical fluids in the dairy industry 12.9 Conclusions and future trends in the dairy industry 12.10 The egg products industry and composition of egg products ... and composition of dairy products 12.3 Pretreatment of milk using separation techniques 12.4 Standardization and concentration of milk proteins in the dairy industry 12.5 Isolation of ... the food and biotechnology industries: principles and applications Edited by A S Grandison and M J Lewis 28 Handbook of indices of food quality and authenticity R S Singhal, P K Kulkarni and D V...

Ngày tải lên: 20/09/2015, 01:57

... PEST1, PEST2 and RING finger) using StuI and HindIII, and MBP-XD1 (1–301 residues of xXIAP and an extra residues derived from an alternative reading frame, lacking RING finger and a part of the BIR3 ... carefully removed and used for further analyses Egg, sperm and extract manipulation Priming and ovulation of female Xenopus frogs and preparation of egg CSF-arrested extracts and demembranated ... sequences of Xenopus BIR family proteins (A) Amino-acid sequence alignment of xSvv1, xSvv2, and human survivin (B) Amino-acid sequence alignment of xEIAP ⁄ XLX, xXIAP, and human XIAP Identical and...

Ngày tải lên: 30/03/2014, 16:20

... optimal temperature of 65 ◦ C and activity of 900 AGU/g protein An amyloglucosidase unit, AGU, is the amount of enzyme able to hydrolyze ␮mol of maltose per minute at 37 ◦ C and a pH of 4.3 Ethanol ... Extraction of anthocyanins and subsequent hydrolysis of ISP residues The analysis of variance (ANOVA) for the main and interaction effects of solvent type, extraction temperature and solid loading ... key to the extraction and hydrolysis processes Assessment of statistical significance was made at an ˛ value of 0.05 2.4 Extraction of anthocyanins and subsequent hydrolysis of ISP residues ISP...

Ngày tải lên: 05/05/2014, 08:39

... in 0.2 ml of TM buffer Expression and purification of proteins VP51 and VP76 are the products of ORF wsv308 and wsv220 of WSSV (GeneBank accession no AF332093) and composed of 466 and 674 amino ... understanding of the molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection in the future Conclusion The localization of the two proteins, VP51 and ... location of VP51 and VP76, and all experimental evidence indicated that VP51 and VP76 are viral capsid proteins http://www.virologyj.com/content/3/1/76 Results and discussion Identification of VP51 and...

Ngày tải lên: 20/06/2014, 01:20

... Hsp 90 ER ER Vtg and Zr protein ERE Vtg and Zrp genes Secretion of Vtg and Zr protein Vtg and Zrp mRNA Transport to ovary and incorporation into oocytes Figure Simplified diagram of estradiol-17β ... Recent advances in studies of the organization of eukaryotic genomes have also focused attention on the importance of structural features of expressed and unexpressed genes and on the post-transcriptional ... the chosen standard Generally, this is not the case, and some laboratories develop their own "gold standard" of Vtg, which is used as the standard in quantitation Again, this gold standard needs...

Ngày tải lên: 13/08/2014, 13:20

... UBPs and the TF that is located at the C terminus of Cub will be cleaved off and result in downstream reactions, which is the activation of LacZ and HIS3 reporter genes [7] 3.2 Identification of ... first round of screening Half of the colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting ... Results and Discussion 3.1 Principle of Split-Ubiquitin Screening System Studies have shown that the use of yeast two hybrid system, which is a genetic assay for in vivo detection of interactions of...

Ngày tải lên: 02/11/2012, 11:08

... the elution profiles The profile of steaming-extracted polymer (A) contained two saccharide peaks (at elution volumes of 70 ml and 90 ml) and one UV-absorbing peak (at elution volume of 95 ml) The ... a flow rate of ml/h and ml fractions were collected The void volume of the column was determined by the elution of Blue Dextran (Pharmacia) Other analyses - 147 - Journal of Water and Environment ... reagent grade Results and Discussion Yields and chemical components - 148 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Table Yields and Chemical Components of Polymers Extracted...

Ngày tải lên: 05/09/2013, 09:08

... The order and directions for folding are documented in the functional specification After completing the above steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional ... 104 Activity 12.1: Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct...

Ngày tải lên: 24/01/2014, 10:20

... between two atoms (i,j), of types ti and tj, and Nobs(dij,ti,tj) represents the number of atoms of types ti and tj observed in the structure training set, separated by a distance of at least dij As ... universally conserved component of the core of the signal recognition particle (SRP) The structure of the complex (PDB code: 1HQ1) and the binding affinity of a series of RNA mutants have been determined ... contact cut-off To evaluate the influence of different cut-off values in our study, replicate experiments were conducted ˚ ˚ ˚ using A, 10 A and 12 A distance cut-offs In nearly all of our tests,...

Ngày tải lên: 18/02/2014, 16:20

... nuages of PGCs of medaka embryos and the granules in HeLa cells FRAP revealed that it moved with two diffusion components in both PGCs and HeLa cells: 0.15 and 0.01 lm2ặs)1 in PGCs, and 0.9 and ... analysis of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized ... and to measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and...

Ngày tải lên: 18/02/2014, 16:20

... mapped onto the LRR and AXH domain of Anp32 and Atx1 respectively, and was shown to be stronger for expanded Atx1 [16,18] The temporal and cell-specific expression pattern of Anp32 in cerebellar ... understanding of how interactions could take place in different regions of the molecule As part of a long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ ... Leu128 to Asp146, and includes h6, which belongs to the fifth LRR, and the short strand b6, which runs parallel to b5 and is antiparallel to b7 The solution and the crystal structures of the Anp32a...

Ngày tải lên: 18/02/2014, 17:20

... sequence of about 90 amino acids of unknown function The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% identity) argues for a conservation of the tertiary structure and ... conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment revealed an ... edge and postspawning gonads Metamorphosis represents the ultimate stage of oyster development, and is characterized by the degeneration of larval tissues, such as the velum and the foot, and...

Ngày tải lên: 19/02/2014, 00:20

... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were analyzed as arithmetic means of separate gel runs The number of gel runs ... SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-terminal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs 6H4, SAF60, SAF70 and...

Ngày tải lên: 19/02/2014, 02:20

... [39] and are associated with aggregates in the brains of HD transgenic mice [40] Hsp70 and Hdj-1 can inhibit polyQ aggregation and reduce the size of htt-polyQ inclusion bodies [15,36,37] and ... the morphology of mitochondria (not shown) Huntingtin inclusions and oxidation Comparative analysis of the protective effect of NAC and Hsps against ROS, protein oxidation, iron and nitric oxide ... a constant level of expression of httEx1-polyQ-EGFP polypeptides in the presence of NAC (not shown) and, as shown in Fig 1, NAC did not change the size and EGFP fluorescence of inclusion bodies...

Ngày tải lên: 19/02/2014, 06:20

... Selection of folded proteins by phage-display (Eur J Biochem 271) 1611 Fig Effect of structural change on the selection (A) Structure of cyt b562 Residues M7 and H102 are the ligands of heme Heme ... positions 102 and 106 Nevertheless, the selected proteins were thermodynamically very stable The structure of one of the selected proteins with Ile, Asn and Gly at positions of 98, 102 and 106, was ... Rescue of a multiply mutated and destabilized variant of ubiquitin Biochemistry 38, 11613–11623 13 Lazars, G.A., Desjarlais, J.R & Handel, T.M (1997) De novo design of the hydrophobic core of ubiqutiin...

Ngày tải lên: 19/02/2014, 12:20

... ÔStainsAllÕ analysis of Fig and the 1D immunoblotting of Fig As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of a-dystroglycan ... listed in Table The pH values of the first dimension gel system and molecular mass standards (in kDa) of the second dimension are indicated at the top and on the left of the panels, respectively ... pattern and often result in a streaky spot pattern Therefore, silverstained 2D patterns of muscle proteins probably overestimate the presence of soluble proteins and underestimate the expression of...

Ngày tải lên: 19/02/2014, 16:20

... ’s for each edge of the graph 6: Refine the scores S1 and S3 of the k2 sentiment and topic word candidates using Eqs (5) and (6) iteratively 7: Select k1 new sentiment words and k1 new topic words ... candidates and words in the lexicon C, and the other set of nodes represents sentiment words, including new sentiment candidates and words in the lexicon B O P For a pair of sentiment and topic ... metric of quadratic combination (Zhang and Ye, 2008): S3 (wk ) = (S2 (Rj ) × S1 (wi )) , (3) Rj ∈A, wi ∈B where B is a set of sentiment seeds and A is a set of patterns which the words wi and wk...

Ngày tải lên: 19/02/2014, 19:20

... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... speculation and negation scopes could be concisely expressed as a 284 combination of lexical and syntactic patterns For ¨ u example, Ozg¨ r and Radev (2009) examined sample BioScope sentences and developed ... also manually compiled and used in a number of hedge scope detection systems, e.g (Kilicoglu and Bergler, 2008), (Rei and Briscoe, 2010), (Velldal et al., 2010), (Kilicoglu and Bergler, 2010), (Zhou...

Ngày tải lên: 20/02/2014, 04:20

... morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair of the surface string and the part -of- speech of mi The similar morpheme feature ... 230–237 John Lafferty, Andrew McCallum, and Fernando Pereira 2001 Conditional Random Fields: Probabilistic Models for Segmenting and Labeling Sequence Data In Proceedings of ICML, pages 282–289 ... both features of original morphemes and features of similar morphemes The following subsections describe these steps respectively 3.1 Assignment of Similar Morpheme A context vector Vm of a morpheme...

Ngày tải lên: 20/02/2014, 09:20

... form of b strand [18] The positive bands at » 1675 cm)1 in the amide I region of the ROA spectra of b- and j-casein, which originate mainly in the peptide C ˆ O stretch, are characteristic of disordered ... types of loops and turns A negative ROA band in the region » 1238±1253 cm)1 appears to be a reliable signature of b strand, individually or within b sheet, so the well-de®ned negative band at ... the theme of PPII structure and rheomorphism is explored by a comparative ROA study, supplemented with DSC, of caseins, synucleins and tau, together with several mutants of a-synuclein and tau...

Ngày tải lên: 21/02/2014, 15:20