... (23) The area enclosed by the solvent circuit in the TS diagram represents the thermodynamic work needed forthe process of cycling the SCF The lower the pressure drop needed for separating the ... critical point ofthe other component and then rising sharply with pressure, whereas the other line goes from the critical point ofthe other component to the UCEP Various sub-classes of type III ... mixture of compounds has to be high whereas in another stage, the solubility of a compound in SCF has to be low Therefore, the phase equilibrium data is the most important factor in the design of the...
... important for caspase inhibition Some members also contain the C-terminal RING finger domain, which is reported to be required forthe ubiquitylation of themselves and bound caspases [26–28] They are therefore ... (Fig 3B) However, these proteins may be unstable and completely degraded before the onset of apoptotic change To examine the stability of these proteins, we first tried to detect the endogenous molecules ... be further degraded by the N-end rule pathway in Drosophila [43] To evaluate the contribution of these proteolytic systems, the effects of specific protease inhibitors were tested against the degradation...
... content for purple-fleshed ISPs was on average 89.7% ofthe dry matter Change in starch content ranged from 67 to 78.3% ofthe dry matter, leaving a residual starch content of at least 11.4% ofthe ... ethanol production from ISP extraction residues Since the majority of sugars produced from residual solids that underwent anthocyanin extraction were in the form ofthe simple sugar glucose, the ... 0.05) At 50 ◦ C there was no statistical difference in the type of solvent used; however, for 25 ◦ C both ofthe acidified solvents had statistically higher phenolic concentrations than the non-acidified...
... proteins The results may facilitate a better understanding ofthe molecular mechanism of WSSV infection and assembly, or be helpful forthe control of virus infection in the future Conclusion The ... now, the pathogenesis of WSSV has not been clearly understood on the molecular level Thus there is an urgent need to study the structural proteins and their function of this virus to find out the ... technique forthe localization ofthe structural proteins of WSSV or other large viruses due to its facility, high efficiency and sensitivity All in all, WSSV is most virulent pathogen ofthe penaeid...
... silencing of at least nine ofthe ten pseudogenes (ibid.) It has become increasingly clear that the proteins ofthe zona pellucida are conserved among eutherian mammals and that the proteins ofthe ... body) Towards the end ofthe vitellogenic period, or by the beginning ofthe final maturation, the germinal vesicle (nucleus), which in the early stages is centrally located, moves to the periphery ... differences in the structure of zona radiata interna [65] Since 1989, several reports have demonstrated the hepatic synthesis of precursor proteins ofthe inner layer subunits under the influence of estrogen,...
... selected forthe first round of screening Half ofthe colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity ... check forthe presence of haploids The culture was split into two 50ml Falcon tubes and centrifuged at 1000 x g for 10 minutes The 3-litre flask used for mating was rinsed twice with 50ml of 0.5X ... done for 22 randomly picked colonies to assess the size of cDNA insert For each colony picked, half was streaked on SD/-Trp plate and the other half inoculated into a 20µl PCR mix Three ml of the...
... of 95 ml) The A280 to A260 ratio ofthe UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile ofthe NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... control test was calculated every minute using the following formula: The relative A660 = A660 of flocculating test A660 of control test Forthe control, the polymer solution was replaced by distilled ... compared to the other methods The SDS-extracted polymer included a small amount of material other than protein Furthermore, the steaming- and washing-extracted polymers contained large amounts of phosphorus...
... lines to create the boat The order and directions for folding are documented in the functional specification After completing the above steps, you will discuss the activity with the class Activity ... Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct an origami boat using the sheet distributed by the instructor Fold the paper along the ... completing the above steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS PAGE INTENTIONALLY LEFT BLANK ...
... on the presence or absence of a cytosine at position )5 in the RNA A likely explanation forthe importance ofthe )5 cytosine mutation is offered by the observation that the amino group ofthe ... onto the RNA bases nearest the center ofthe b-sheet structure ofthe RRM We then scored these sequence-variant structures with the distancedependent potential function Figure shows the results of ... the highresolution modeling of protein–RNA interactions The anticipated future enrichment ofthe structural database will further improve the predictive performance ofthe potential Results The...
... of OlvasGFP reveals the dynamic nature ofthe nuage Schematic diagram ofthe preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the ... outside ofthe nuage in the cytoplasm ofthe migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement ofthe PGC, the 10 ... analyses of OlvasGFP in the PGC FCS was used to measure the movement of OlvasGFP into the cytosol out ofthe nuage region ofthe PGC Representative correlation curves are shown (A) The measurement...
... accurate definition ofthe C-terminal domain boundary Experimental Study ofthe interactions ofthe Anp32a LRR module determination ofthe dynamic features ofthe domain in solution, together with a ... within the range expected for a single monomeric species of this size in solution [29] The flat profile ofthe relaxation parameters along the sequence indicates that, with the exception ofthe first ... Study ofthe interactions ofthe Anp32a LRR module C de Chiara et al A B Fig Modeling the interactions ofthe Anp32a LRR domain (A) Structure ofthe Anp32a LRR domain in a complex with the C...
... and the remodelling of larval tissues to produce adult tissues (i.e the development ofthe gills and theproductionof an adult shell), which is accompanied by significant growth ofthe soft body ... C-terminal sequence of about 90 amino acids of unknown function The high degree of identity ofthe Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% identity) argues for a conservation ofthe tertiary structure ... based on the conserved amino acid sequences ofthe catalytic domain of members ofthe GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment...
... used for PrP detection Calculation ofthe banding patterns of 17 gels using antibody SAF34 gave an SE of 2.1 forthe di-glycosylated isoform, 1.0 forthe mono-glycosylated band and 1.6 forthe ... calculation ofthe percentages ofthe di- (d), mono- (j) and nonglycosylated (m) isoform as arithmetic means of separate gel runs The number of gel runs forthe analyses are given for each antibody ... (calculated here for murine PrP) [18] Taken together, the results of various signal intensities ofthe three PrPC bands are accredited to the development ofthe truncated isoforms, to the epitope...
... ofthe inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution ofthe size ofthe inclusions were dependent on when the analysis was performed ... (NAC) before being analyzed The arrow indicates the position ofthe more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) ofthe oxyblots ... problem that the cell has to challenge to promote the formation and clearance of these bodies Aggregate clearance could therefore be balanced between the rate of huntingtin aggregation and the deleterious...
... effect on the result of selection If the cutting sites are in the flexible loop ofthe native structure, they could prevent the selection of stable proteins By examining the structures ofthe proteins ... [1,13,22,27,28] The advantage ofthe computational methods is that they can examine very large numbers of mutations [27] The limitation ofthe current computational methods, however, is that most ofthe ... substituting forthe two hydrophilic residues, an interpretation forthe selection result is proposed In the X-ray structure, the hydrophobic interaction distorted the last turn ofthe C-terminal...
... to the Dp427 isoform of dystrophin (A), the a-subunit ofthe dystroglycan complex (a-DG; B), the Na+/K+ ATPase (NKA, C), the fast SERCA1 isoform ofthe sarcoplasmic reticulum Ca2+ ATPase (D), the ... with both the 2D ÔStainsAllÕ analysis of Fig and the 1D immunoblotting of Fig As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression ... In contrast, the expression of major ion-regulatory muscle components, such as the Na+/K+ ATPase, the SERCA1 isoform ofthe sarcoplasmic reticulum Ca2+ ATPase, and the a1-subunit ofthe dihydropyridine...
... experiments to study the effect of different parameter settings There are several parameters in the framework: the number of generated seeds r, the number of new candidates k2 and the number of selections ... word extraction, the results ofthe proposed methods are not sensitive to the values of r While forthe topic word extraction, the proposed methods perform well when r falls in the range from 15 ... trees forthe sentence the camera is great” in the camera domain and the sentence the movie is excellent” in the movie domain, respectively As can be observed, the relationships between the topic...
... Statistics ofthe BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% ofthe ... by matching the path of cue leaf nodes to the root ofthe rule subtree pattern If an identical path exists in the sentence, the root ofthe candidate subtree is thus also identified The candidate ... developed a supervised classifier for identifying speculation cues and a manually compiled list of lexico-syntactic rules for identifying their scopes Forthe performance ofthe rule based system on identifying...
... corpus The methods with SF outperform the ones without SF Furthermore, performance improvements between the ones with SF and the ones without SF are greater than Table The performance of CRF ... morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair ofthe surface string and the part -of- speech of mi The similar morpheme feature ... from the label B and the label I for eight NE types, in order to distinguish them When the task of extracting Japanese NEs from a sentence is formalized as a chunking problem of a sequence of morphemes,...
... repeats Three isoforms have three repeats each and the other three isoforms have four repeats each Depending on the isoforms, tau has either one (threerepeat forms) or two (four-repeat forms) cysteine ... corresponds to the 412-amino-acid isoform of human brain tau At neutral pH, the tau samples showed aggregation in the laser beam, with the aggregates falling to the bottom ofthe cell, so that the concentration ... reveals that the /,w angles of most ofthe residues ofthe Bowman±Birk inhibitor are distributed fairly evenly over the b- and PPII-regions ofthe Fig A MOLSCRIPT diagram [67] ofthe X-ray crystal...