2 targeting hdac activity in animal models of arthritis

... compare the incidence and severity of PGIA and proteoglycan-induced spondylitis and to determine expression patterns of a panel of relevant genes in a large number of distinct colonies of the susceptible ... rheumatoid arthritis Curr Opin Rheumatol 2006, 18:141-146 van der Helm-van Mil AH, Huizinga TW: Advances in the genetics of rheumatoid arthritis point to subclassification into distinct disease ... subsets Arthritis Res Ther 2008, 10:205 Holmdahl R: Dissection of the genetic complexity of arthritis using animal models Immunol Lett 2005, 103:86-91 Rosenthal N, Brown S: The mouse ascending:...

Ngày tải lên: 09/08/2014, 14:20

... possible An alternative mode of imaging is the use of single photon imaging of γ-emitting isotopes like 11 1In or 99Tc Imaging of γ-emitting isotopes is referred to as single photon emission commuted ... be collected in a couple of minutes of recording, the migration of leukocytes in tissues is relatively slow and requires many minutes of recording to track cells This longer imaging period requires ... signs of inflammation The groundwork for further studies on the dynamics of lymphocyte interactions in the synovium has thus been established 168 Most of the work in intravital imaging of leukocytes...

Ngày tải lên: 09/08/2014, 01:22

... currently under investigation in animal models of arthritis This review focuses on the gene therapeutic strategies of restoring the cytokine balance and genetic synovectomy in animal models of RA, as ... reduction in local production of proinflammatory cytokines, thereby reducing their circulating levels and abolishing a sensitizing effect on naïve joints A conflicting finding was the protection of ... DT, Martin MU, van den Berg WB: Modulation of arthritis through overexpression of soluble interleukin-1 receptor accessory protein: a novel inhibitor of interleukin-1, distinct from IL-Ra Inflammation...

Ngày tải lên: 09/08/2014, 01:24

... significantly inhibited FGF8-induced incorporation of [3H]thymidine Articular destruction by intraarticular injection of FGF8 into the rat knee joint The activity of FGF8 on the knee joint was tested ... Figure Intraarticular injection of FGF8 induced the sulfated glycosaminoglycan release in knee joints and destruction of patella Fifty micrograms of FGF8 patella was injected into the knee joint of ... specifically out of the four human FGF8 isoforms, and showed little binding to other members of the FGF family Neutralizing activity of KM1334 was shown by the blocking of FGF8b binding to its receptors...

Ngày tải lên: 09/08/2014, 10:23

... [25,26] CARR injection in the hind paw of the mouse is one of the most commonly used models of inflammation and inflammatory pain and it has a biphasic profile [27] Recent studies pointed out important ... prepared in saline 30 μL of saline were injected as control in the controlateral paw No major adverse effects were observed after injection of 2% carrageenan in the right paw of the mice All the animals ... processing of other cytokines and cytokine receptors [32-35] Interestingly, PTPH1 is known to inhibit TACE expression and activity in vitro [36] We therefore analyzed cytokines plasma levels in carrageenan-treated...

Ngày tải lên: 11/08/2014, 03:20

... proteins include protein serine-threonine kinases (Akt and phosphoinositidedependent kinase (PDK1)), protein tyrosine kinases (Btk and Tec family), exchange factor for GTP-binding proteins (Grp1 ... Inhibition of cytokines (e.g IL-4, IL-5, and IL-13) is a promising way of obtaining efficacious drugs for asthma There are several potential ways of inhibiting cytokine effects including blocking antibodies, ... Tyrosine kinase signaling cascade 1.2.1 Protein tyrosine kinases (PTKs) Protein tyrosine kinases are enzymes that carry out tyrosine phosphorylation by catalyzing the transfer of the γ phosphate of...

Ngày tải lên: 15/09/2015, 22:09

... examination of inflammatory cell infiltration into the trachea in guinea pigs 4.9E-H 80 Effects of genistein on EPO activity in cell-free BAL fluid in guinea pigs 4.9A-D 79 Effects of genistein or ... daidzein on OVA-induced BAL fluid eosinophilia in guinea pigs 4.8 78 86 Effects of genistein on EGF-induced protein tyrosine phosphorylation in guinea pig lung fragments 88 5.1 OVA-induced cell infiltration ... effects of the intervention of these signaling pathways in animal models of asthma The purpose of my thesis was to investigate the antiinflammatory effects of genistein, a broad-spectrum protein tyrosine...

Ngày tải lên: 16/09/2015, 17:11

... activation resulting in innate cell mediator activation and the production of inflammatory cytokine interleukin-1 and TNF-α, leading to joint destruction In recent studies, mice depleted of macrophages ... More importantly, knocking out the expression of gelsolin, an actin-binding protein with filament-severing properties found downregulated in RA, resulted in mild exacerbation of the arthritic phenotype ... a single injection of pristane subcutaneously [3] This contrasts with murine pristaneinduced arthritis, where a systemic granulomatous disease, including arthritis, occurs after repeated injections...

Ngày tải lên: 09/08/2014, 01:23

... identified TNF/IL-1 involvement In 1991, Kollias and coworkers provided insight into the possible role of TNF in arthritis induction in an elegant series of experiments By introducing into mice a modified ... IL-1 is not a dominant early inflammatory cytokine in all models, it is the pivotal cytokine in inhibition of chondrocyte proteoglycan synthesis in the articular cartilage in all models studied ... is less in arthritis in IL-1ra-/- mice and virtually absent in TNFtg mice, both models showing a gradual but slow progression of arthritis Of great interest, a TNF-inducible regulator of bone...

Ngày tải lên: 09/08/2014, 14:22

... increase brain levels of 'free CRF' in Alzheimer's disease (AD) and show cognition-enhancing properties in models of learning and memory in animals without the characteristic stress effects of ... currently in late phase II trials for the treatment of anxiety It appeared to have an anxiolytic effect in a variety of tests on animal models, including punished drinking tests, social interaction ... especially interesting in view of the coexistence of CCK and dopamine in midbrain neurons and the regulation of mesolimbic dopaminergic pathways by both CCK1 and CCK2 receptors 2.3 Function of CCK-related...

Ngày tải lên: 17/09/2015, 17:20

... 1391 Animal models of Alzheimer’s disease O Philipson et al Transgenic animal models Models devoid of any disease-causing APP mutations Animal models expressing wild-type (wt) human APP are of interest ... important in minimizing the in uence of confounding factors arising from inherent problems associated with breeding APP transgenic mice and maintaining stable phenotypes We would argue that a single ... similar in single- and doubletransgenic mice [96] The findings are relevant to the Lewy body variant of AD with a-synuclein inclusions Instead of cross-breeding two single-transgenic models, several...

Ngày tải lên: 16/02/2014, 09:20

... but were mainly clustered with intermediate sensitivity AML Interestingly, introducing both CD34+ and CD33+my cell sensitivity to a-bisabolol (as the mean value of cases) in clustering analysis ... proliferation of the cell line Means ± SD of experiments Right side Plot showing the corrisponding combination index (CI) vs the fraction affected (Fa) CI values are

Ngày tải lên: 18/06/2014, 19:20

... BH, Kanter JL, Steinman L, Robinson WH: Epitope spreading to citrullinated antigens in mouse models of autoimmune arthritis and demyelination Arthritis Res Ther 2008, 10:R119 Kinloch A, Lundberg ... Antibodies against citrullinated proteins enhance tissue injury in experimental autoimmune arthritis J Clin Invest 2006, 116:961-973 Vossenaar ER, Van Boekel MA, Van Venrooij WJ, Lopez-Hoyoz M, Merino ... Lopez-Hoyoz M, Merino J, Merino R, Joosten LA: Absence of citrulline-specific autoantibodies in animal models of autoimmunity Arthritis Rheum 2004, 50:2370-2372 Page of (page number not for citation...

Ngày tải lên: 09/08/2014, 13:22

... Anti-inflammatory effects of KHU14 on animal models of acute inflammation Anti-inflammatory effects of KHU14 on animal models of acute inflammation (A) Ear edema in mice (n = 10) (B) Paw edema in ... effects of KHU14 on acute inflammation We employed three animal models of acute inflammation to evaluate the anti-inflammatory effects of KHU14: (1) inhibition of croton oil-induced ear edema in mice; ... Thirty minutes after the administration, each mouse was intravenously injected (at the tail) with 0.1 ml of 4% Evans blue (10 μl/g of body weight, Sigma, USA) in saline Fifteen minutes after the intravenous...

Ngày tải lên: 13/08/2014, 15:21

... Department of Genome Sciences, University of Washington, Seattle; 2005 Johnson JD, Misler S: Nicotinic acid-adenine dinucleotide phosphate-sensitive calcium stores initiate insulin signaling in human ... suggest an increased risk of type diabetes as a consequence of low adiponectin levels [24] In the CGC application, CDH13 obtains 38.9 points from only one single matching keyword ("adiponectin") However, ... metabolic syndrome, including visceral obesity, insulin resistance and dyslipidemia [25,26] In CGC, HSD11B2 is ranked tenth, obtaining 35.1 CGC points due to as much as nine matching keywords, although...

Ngày tải lên: 13/08/2014, 16:21

... CA) Animals and cell lines BALB/c mice were purchased from the West China Experimental Animal Center Murine breast carcinoma cell line 4T1, colon carcinoma cell line CT26 and endothelial cell line ... extensively studied in animal models and clinical trials Anti-cancer vaccine strategies have included immunization with whole-tumor cell vaccines, peptide vaccines, dendritic cell (DC) vaccines, viral ... following immunization (Figure 4) In contrast, the sera isolated from controls showed negative staining Inhibition of angiogenesis Vaccination with human VEGFR-2 LDC resulted in the apparent inhibition...

Ngày tải lên: 14/08/2014, 19:22

... preprocholecystokinin The signal peptide consists of residues 20 to The amino terminal flanking peptide consists of residues to 25 The largest characterized form from brain and intestine, CCK-58, consists of ... Figure 1-6 Interactions between mammalian corticotropin releasing factor (CRF)like peptides, their receptors and the CRF binding protein (CRF-BP) Binding to the CRF-BP or activation of CRF1 or ... of CCK4 on context- and tone-dependent fear conditioning after i.h injection Mice were injected with 50ng CCK4 30min before training Context- and tone-dependent fear conditioning were determined...

Ngày tải lên: 17/09/2015, 17:20

... Role of CRF2 receptors in anxiety 35 Table of Contents 4.5 Role of the CRF system in memory and learning Animal Models vi 37 39 5.1 Introduction 39 5.2 Validity criteria of an animal model 40 5.3 ... (Glu11,16)-astressin I.c.v injection of CCK4 before training, but not after training, reduced the freezing behavior of mice on both context- and tone-dependent fear conditioning This impairment ... Pharmacology of CRF1 and CRF2 receptors 28 29 4.3 CRF-binding protein 30 4.4 Role of the CRF system in anxiety 31 4.4.1 CRF and anxiety 31 4.4.2 Role of CRF1 receptors in anxiety 33 4.4.3 Role of CRF2...

Ngày tải lên: 17/09/2015, 17:20

... nucleotide cofactor binds in an extended conformation at the top of the a ⁄ b-barrel, with the nicotinamide ring projecting down into the center of the barrel and pyrophosphate straddling the barrel ... nmolÆmin)1Æ(mg protein))1, respectively (Fig 1B) AKR1B3 with 85.8% identity of the amino acid sequence of AKR1B1 also catalyzed both PGFS activity [3.6 nmolÆmin)1Æ(mg protein))1] in the presence of ... and PGFS activities of recombinant AKR1B1 protein (A) Autoradiogram of TLC after incubation of AKR1B1 (each 10 lg protein) with lM 1-[14C]PGH2 in the presence or absence of cofactor at 37 °C for...

Ngày tải lên: 14/03/2014, 23:20