2 sp cells side population cells

... trypsinization BV-2 cells were seeded onto 96-well plates (104 cells/ well for cell viability assay), 24-well-culture plates (105 cells/ well for ELISA and NO measurement, 104 cells/ well for immunofluorescence), ... plates (2.5 × 105 cells/ well for PCR) or 100 mm culture dishes (1.2 × 106 cells/ dish for western blotting and EMSA) Before each experiment, cells were serum-starved for 12 h BV-2 cells were incubated ... was considered statistically significant Results ALXs are expressed in BV-2 microglial cells Using RT-PCR, we showed that both ALX1/FPR-rs1 and ALX2/FPR2 were expressed in BV-2 microglial cells...

Ngày tải lên: 19/06/2014, 22:20

... used for bacterial invasion studies, the cells were diluted 1:2, seeded in 24 well plates (Corning) and incubated over night at 37°C The following day, cells were counted using a Bürker chamber ... HEp-2 cells grown over night in 24 well plates, to a multiplicity of infection (MOI) of approximately 100 bacteria pr cell Plates were incubated for 90 at 37°C and MEMs was removed The cells ... 37°C for two hours MEMg was removed; cells were washed three times with PBS de Boer and lysed with ice-cold 1% Triton-X (Sigma-Aldrich) in PBS de Boer for 10 The cells were dislodged using a sterile...

Ngày tải lên: 12/08/2014, 18:22

... SILICON RIBBON m e lt CASTING Cast mc-Si solar cells END PART-II CONCEPTS OF HIGH EFFICIENCY SILICON SOLAR CELLS (LOSSES IN SOLAR CELLS) LOSSES IN SOLAR CELLS • THERMODYNAMICAL • OPTICAL • ELECTRICAL ... DIFFERENT COMPONENTS Burried Contact Silicon Solar cells Green et al (1988) High Efficiency Silicon Solar Cells (for normal sunlight: 1sun) These cells have effective BSF ( d/L

Ngày tải lên: 27/01/2016, 16:11

... denotes a D-mannopyranose unit The side- chain sequence is not specified The molar ratio of the side chains in the mannan is expressed as a percentage of the total side chains The values are calculated ... synthesis of these side chains recognizes the length of the side chain from the a-1,6-linked backbone mannose units If we use the backbone a-1,6-linked mannose-containing side- chain oligosaccharide ... polyclonal antibodies to Candida cells, were purchased from Iatron (Tokyo, Japan) Except for factor serum, which is unabsorbed rabbit whole-cell serum against C albicans cells, factors 4, 5, 6, and...

Ngày tải lên: 17/03/2014, 03:20

... of viable cells were observed for both cell lines attached to plastic However, when the cells were attached to monomeric 2D collagen, the number of viable cells increased, whereas for cells attached ... pHb-1 cells while filled boxes denote II-11b cells Lines inside the boxes indicate median values, and dotted lines illustrate mean values (n = 12) (B) Harvested media from pHb-1 and II-11b cells ... into the medium from pHb-1 cells compared with II-11b cells when attached to plastic (5.0 versus 3.1 lgÆmL)1Æ106 cells) 1) (Fig 6) The difference was sustained when cells were attached to fibrillar...

Ngày tải lên: 18/02/2014, 11:20

... adenocarcinoma, the cells undergo spontaneous enterocytic differentiation and share many characteristics with human small intestinal cells in their differentiated state Caco-2 cells form a polarized ... expression profiles of Caco-2 cells To detect differences in protein expression related to glutamine addition to the Caco-2 cells, proteins were isolated from 5-day-differentiated cells exposed for 24 ... proteins in Caco-2 cells Alternatively, our findings can be explained by the relative high endogenous glutamine synthesis capacity of Caco-2 cells compared to human small intestinal cells [16,44] This...

Ngày tải lên: 20/02/2014, 01:20

... b -cells has traditionally been considered to be the mitochondrial electron transport chain, recent attention has focused on NOX enzymes as a potential source of ROS production in pancreatic b -cells, ... show that exposure to d-glucose led to dysfunction of NIT-1 cells We next assessed whether d-glucose induces apoptosis of NIT-1 cells NIT- 1cells were doubly stained using Annexin V and PI kit Annexin ... of NIT-1 cells induced by d-glucose Suppression of NOX2 substantially restores dysfunction and apoptosis of NIT -cells D-glucose-induced P38MAPK and p53 mediate the apoptosis of NIT-1 cells induced...

Ngày tải lên: 06/03/2014, 02:20

... RINm5F cells after hA treatment (data not shown) However, no increment in p-ATF-2 level was detected in cells treated with either vehicle alone or noncytotoxic rA in either RINm5F or CM cells, ... treated CM cells Cells were transfected with a c-jun promoter-CAT reporting construct for 24 h before exposure to hA, rA or vehicle control (co) for various times as indicated Transfected CM cells ... rat b -cells [10,15,16] In addition, onset of diabetes, associated with islet amyloid formation and decreased b-cell mass, has been demonstrated in transgenic mice expressing hA in their b-cells...

Ngày tải lên: 07/03/2014, 12:20

... 222 in PC12 cells (A) NGF-induced differentiation of PC12 cells (B) Schematic representation of the genomic structure of miR-221 and 222 and their corresponding sequences (C, F) PC12 cells were ... PC12 cells, NGF stimulation of starved cells promotes cell survival and differentiation, and inhibits cell-cycle progression [16] However, NGF-induced miR-221 and miR-222 expression in PC12 cells ... well) were co-transfected with the internal control plasmid (20 ng per well) into PC12 cells After 24 h, the cells were harvested, and the lysates were subjected to a luciferase assay The results...

Ngày tải lên: 16/03/2014, 01:20

... observed in RIN-m5F cells treated with STZ (1.25–20 mm) for h (data not Fig STZ-cytotoxicity in RIN-m5F cells (A) LDH release from RIN-m5F cells pretreated with STZ RIN-m5F cells were preincubated ... measurement of LDH released from dead cells, due to the loss of cell membrane integrity observed in both necrotic and apoptotic cells The long-term exposure of RIN-m5F cells to STZ at concentration of ... death of b cells We previously demonstrated that exposure to streptozotocin (STZ) induced significant cytotoxicity and apoptotic cell death in RIN-m5F cells (i.e the rat insulinoma cells) in which...

Ngày tải lên: 16/03/2014, 04:20

... of butan-1-ol Cells were subcultured in six-well plates at · 105 cells per well and serum-starved in the presence of lCiÆmL)1 [3H]myristic acid After overnight starvation, the cells were washed ... stimulates PLD activity in U87 human astroglioma cells We investigated whether green tea polyphenols activate PLD in U87 human astroglioma cells Cells were treated for 30 with EGCG, ECG, EGC or ... inhibited PLC activity in cells stimulated by EGCG (Fig 3C) We found that PLC-c1 was the predominantly expressed PLC in U87 cells, indicating that the PLC activity shown in these cells may be due mainly...

Ngày tải lên: 16/03/2014, 16:20

... apparatus These include studies on rat mast cells [15], Chinese hamster ovary cells [16,17], rat alveolar epithelial cells [18] and rat glomerular epithelial cells [19] More recently, studies using ... type EA.hy.926 cells are a hybrid derived from HUVEC and A549 cells [30] In order to establish the validity of the EA.hy.926 cells as a model for studies on cPLA2-a in endothelial cells, expression ... endothelial cells (HUVECs) and rabbit coronary endothelial cells have reported a redistribution of cPLA2-a to the nuclear envelope [26,27] The localization of cPLA2-a in human endothelial cells has...

Ngày tải lên: 16/03/2014, 18:20

... 30 000 cells (A) HGC-27 cells were cultured in six-well plates, and after exposure to VD3 for 24 h, cells were collected for quantitative real-time PCR and western blotting (B) HGC-27 cells were ... cells transfected with PTEN siRNA, as compared with cells transfected with control siRNA (Fig 1D) Meanwhile, the early apoptosis induced by VD3 was reduced from 6.06% to 2.12% in PTEN siRNA cells ... apoptosis in acute lymphoblastic leukemia cells [27] To evaluate the contribution of 5Aza to the regulation of PTEN in gastric cancer cells, we treated HGC-27 cells with 5-Aza; as a result, both Egr-1...

Ngày tải lên: 22/03/2014, 21:20

... is specifically found in ciliated epithelial cells; however, it is not found in the basal and goblet cells in the epithelium or in the submucosal gland cells [4] Therefore, it is thought that HAT ... phosphorylation of EGFR mediated by AR in NCIH292 cells NCI-H292 cells were stimulated with HAT (200 nM) or AR (3 ngÆmL)1) for the indicated durations (A, B) NCI-H292 cells were pretreated with the vehicle ... NCI-H292 cells were stimulated with PAR-2 AP (300 lM) or HAT (200 nM) for the indicated durations (C) NCI-H292 cells were pretreated with the vehicle alone or with PAR-2 AP (300 lM) for 20 (C) Cells...

Ngày tải lên: 30/03/2014, 11:20

... Library Plate 293-CymR cells in 60-mm tissue-culture dishes at ϫ 105 cells per dish (see Note 3) Incubate overnight at standard conditions Remove the medium and infect the cells with mL of fresh ... transfection mixture to the Ad5-D[PS-E3-E4(ϩorf6)] infected cells 22 Ogorelkova et al Incubate the cells at standard conditions for 3–4 d Collect the cells together with the medium and subject them to ... case, cells can be treated with agents affecting cell viability prior to infection Increased viability will lead to higher GFP intensity Inoculate HeLa cells in 96-well plates at ϫ 105 cells...

Ngày tải lên: 11/04/2014, 00:48

... Wild, the cells without transfection; Transfected, the cells transfected with S ODN (S) or AS ODN (AS) with PAR-2 AS ODN, compared with that of the cells transfected with S ODN or wild cells (non-transfected ... cells without transfection; Transfected, the cells transfected with S ODN (S) or AS ODN (AS) None, the cells without treatment and cultured in the absence of H pylori; H pylori control, the cells ... gastric epithelial cells [21,22] Trypsin activated PAR-2, which mediated the proliferation of various cells including pancreatic and gastric cancer cells, and smooth muscle cells [23-25] Additionally,...

Ngày tải lên: 18/06/2014, 16:20

... int 60 CD3 50 40 TG D0 D7 D14 D21 Cells (x106) 12.3 hi 25 D0 D7 D14 D21 Cells (x106) 125 TG hi CD3 50 Cells (x106) 26.1 15.7 low Cells (x106) 4.3 Cells (x106) B Cells (x106) 125 40 30 20 40 30 ... D7 D14 D21 100 20 low NK cells (x106) 25 100 NK cells (x106) CD3 NK cells (x106) 20 low 30 CD3+CD8+ T cells (x106) 40 35 CD3 CD3+CD8+ T cells (x106) 60 low CD3+CD8+ T cells (x106) CD3 CIK (x106) ... Figure Phenotypic features of TG-expanded CIK cells, NK cells and CD8 T cells The frequency of CD3+CD8+ T cells, NK cells (CD3-CD16 + CD56+) and CIK cells (CD3+CD16+CD56+) was measured by flow...

Ngày tải lên: 18/06/2014, 16:20

... WH7803 host cells was infected with cyanophage strain SPM2 at a MOI of approximately and incubated for 12 hours at 25°C under constant illumination Duplicate mL and mL aliquots of infected cells (for ... extracted as described below Extraction of viral nucleic acid from infected host cells RNA was extracted from mL of pelleted cells using a Qiagen RNeasy® Mini kit according to the manufacturer's instructions ... originally identified in cyanophages in order to develop a PCR-based assay to analyze natural cyanophage populations [21] Page of (page number not for citation purposes) Virology Journal 2007, 4:52 Sequence...

Ngày tải lên: 18/06/2014, 18:20

... transfection, wounds were created in confluent cells using a 200-μl pipette tip The cells were then rinsed with medium to remove any free-floating cells and debris Medium was then added, and culture ... in the percentages of apoptotic cells between the OE21 cells transfected with 50 nM miR205 precursor and anti-miR-205 inhibitor (Figure 3C) Log10 -1 -2 -3 ESCC cells A miR-205 modulates cellular ... malignant cell types examined and Het-1A cells (B) Northern blot analysis showed the intense miR-205 expression in OE21 cells despite its nominal expression in Het-1A cells (C) Consistent with this, knockdown...

Ngày tải lên: 18/06/2014, 19:20

... French-American-British, WBC white blood cells, BMC bone marrow cells, BM bone marrow, PM peripheral blood.*Percentage of CD34+ cells in bone marrow cells of AML patients before sorting # The ... purity of CD34+ cells The percentages of CD34+ cells were 99.43 ± 0.60% in KG1a cells, 96.67 ± 0.11% in Kasumi-1 cells, but CD34+ was absent in U937 cells (Figure 1A) After treatment of these three ... lines to DNR-induced apoptosis (40.15% in KG1a cells and 86.23% in Kasumi-1 cells) , compared to DNR only (3.17% in KG1a cells, 5.94% in Kasumi-1 cells) These results suggest that suppression of...

Ngày tải lên: 18/06/2014, 19:20