2 modulation of gene expression

... the expression of orthologous genes where the expression changes in the two species; the middle panel shows the modulation in the expression of FBT genes; the lower panel shows the expression of ... a majority of genes whose expression was modulated by more than two-fold had increased expression levels in L infantum MTX20.5 but the majority of another set of genes had decreased expression ... resistance The relative expression ratio of each individual gene of chromosomes (a) 22, (b) 28, (c) 11 and (d) 12 of L major MTX60.4 was contrasted with the expression levels of the same genes in L major...

Ngày tải lên: 14/08/2014, 20:22

... MODULATION OF GENE EXPRESSION IN BLADDER CANCER JUWITA N RAHMAT B.Sc (SCIENCE), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF SURGERY NATIONAL UNIVERSITY OF SINGAPORE ... properties and roles of MptpA and MptpB in vitro and in vivo 26 Table 2.1 List of primers for mouse gene expression analysis 50 Table 2.2 List of primers for human gene expression analysis 52 ... Table 6.1 BCG internalization profiles of neutrophils and DCs 140 Table 6.2 Effects of MptpA treatment on DC surface marker expression of CD86 and CD40 143 xiii List of Publications and Conference...

Ngày tải lên: 11/09/2015, 10:06

... and binds to and regulates basal expression via HSEs of target genes [26] Deletion of the HSF gene is lethal to cells, even at physiological temperatures The genes targeted by HSF encode proteins ... trimerization of HSFs is important to establish interactions with the gap- and step-type HSEs The different types of HSEs result in the induction, by yeast HSFs, of different levels of gene expression ... fibre cell differentiation [3] The HSF3 gene was recently identified in mouse as an orthologue of the chicken HSF3 gene; however, the human HSF3 gene is a pseudogene [41] Mouse HSF3 translocates into...

Ngày tải lên: 18/02/2014, 04:20

... Labeling of a PCR-amplified probe with DIG as part of in situ RT-PCR indirect detection of gene expression expression standard RT-PCR was performed showing specific amplification of the TNF gene For ... success of both the DNA elution and re-amplification depends on the amount of cDNA generated during the first round of PCR A good way of optimizing the first round PCR is to take advantage of known genes ... intensity of green versus red This gives a measure of the relative levels of the competing mRNAs bound to each spot and so provides information on the relative level of expression of the genes on...

Ngày tải lên: 25/10/2013, 22:20

... control of gene expression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation rate is essential for the control of the expression of ... activity of both RRMs Finally, the association of ASF ⁄ SF2 with TIAR led us to investigate its role on the expression of reporter gene bearing an ARE in its 3¢ UTR We showed that overexpression of ... might modulate the expression of ARE-containing genes Accordingly, we tested the effect of overexpressing ASF ⁄ SF2 on the expression of Renilla luciferase (Rluc) reporter genes carrying (or...

Ngày tải lên: 16/02/2014, 15:20

... single or multiple gene predictors not involved ‡ in one of the gene expression profile tests of interest: 150 Article does not involve one of the three gene expression tests of interest: 659 No ... later This cut-off year was established based on the introduction date of the MeSH heading gene expression profiling,” 2000, and the introduction date of the MeSH heading gene expression, ” 1990 ... between the investigated gene expression signatures (see Glossary, Appendix B) and the actual gene expressionbased tests The gene “signature” is the collection of genes whose expression levels are...

Ngày tải lên: 06/03/2014, 01:20

... (2005) Analysis of long-lived C elegans daf-2 mutants using serial analysis of gene expression Genome Res 15, 603–615 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression changes ... number of serial analysis of gene expression tag sequences into 3¢ complementary DNAs Genes Chromosomes Cancer 33, 252–261 33 van den Berg A, van der Leij J & Poppema S (1999) Serial analysis of gene ... gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression...

Ngày tải lên: 07/03/2014, 00:20

... of gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression ... known of the function of mRNAs in human spermatozoa, it was difficult to estimate whether the rest of the genes were related to the function of human spermatozoa, or just retained during spermatogenesis ... (2003) An anatomy of normal and malignant gene expression Proc Natl Acad Sci USA 99, 11287–11292 10 Lee S, Zhou G, Clark T, Chen J, Rowley JD & Wang SM (2001) The pattern of gene expression in human...

Ngày tải lên: 07/03/2014, 04:20

... feasibility of analyzing changes in gene expression of articular cartilage two weeks after ACL-transection The specific aims of this study were to determine if changes in relevant gene expression ... content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential gene expression ... Aggrecan expression was increased in the CaLFC and the CrMFC, while Collagen expression was increased in the CaLTP, CaMTP, and CrLTP of ACL-X stifles Col gene expression was upregulated in regions of...

Ngày tải lên: 20/06/2014, 00:20

... μxg represents the mean of xg 2.3 Extraction of Shape Information and Time Scaling To extract shape information of time-varying gene expression, the derivative of the expression trajectory is ... optimal number of clusters was arrived at by multiple runs of the algorithm at values of K, 3.7 Validation of E Coli Expression Data Results We validated the results of our tests using Gene Ontology ... vector of time points associated with gene g, zg is the vector of transformed time-series data (from (1)) associated with gene g, and yg is the resulting vector of first differences associated with gene...

Ngày tải lên: 22/06/2014, 00:20

... evolvability of gene expression Science 2007, 317:118-121 38 Rando OJ, Verstrepen KJ: Timescales of genetic and epigenetic inheritance Cell 2007, 128:655-668 39 Raser JM, O’Shea EK: Noise in gene expression: ... nonadditivity of gene expression in Drosophila melanogaster Genetics 2004, 167:1791-1799 23 Kliebenstein DJ, West MA, van Leeuwen H, Kim K, Doerge RW, Michelmore RW, St Clair DA: Genomic survey of gene expression ... a gene s expression is altered by the environment, and with expression noise [39,40], namely the extent by which a gene s expression differs among genetically identical cells [7,37] That is, genes...

Ngày tải lên: 06/08/2014, 19:21

... with effects of both IFN-α/β and IFN-γ on gene expression In addition to this set of IFN-induced genes, gene expression profiles indicating ischemia and myofiber degeneration and regeneration were ... GeneChips Genes showing at least a twofold difference in comparisons of JDM samples with each of two control samples were used to develop a list of differentially expressed genes Ninety-one genes ... characterize the broad gene expression profile operative in the peripheral blood of patients with SLE [3–6] Most striking is an ‘IFN signature’, a prominent overexpression of mRNAs encoded by genes regulated...

Ngày tải lên: 09/08/2014, 01:23

... limited number of genes Concordance or divergence of results can therefore be estimated only from the selection of genes published in more detail Up to now, gene expression profiling has given ... Assessment of disease activity including organ involvement or destruction is currently limited to general markers of inflammation or organ function and needs profound improvement On the basis of gene expression ... Discovery of distinctive gene expression profiles in rheumatoid synovium using cDNA microarray technology: evidence for the existence of multiple pathways of tissue destruction and repair Genes Immun...

Ngày tải lên: 09/08/2014, 01:23

... molecules of the target gene to the number of molecules of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene The primer efficiencies for the test genes were the same as those for the GAPDH gene ... Treatment with licofelone had little effect on the level of its gene expression or on the level of protein In contrast, the level of expression of mRNA for ADAMTS-4 was found to be significantly increased ... the downregulating effects of licofelone on the level of mRNA expression/ protein synthesis of 5-LOX could provide an explanation of how the drug can reduce the synthesis of MMP-13 and ADAMTS-4 and...

Ngày tải lên: 09/08/2014, 06:23

... of the transcription rates of all genes, accounting for the global regulation of gene expression [22] Gene- specific cis-regulatory elements that mediate rhythmic gene expression might therefore ... overexpression, but a significant non-rhythmic basal level remains These results indicate that KaiC (probably as part of a complex) coordinates genome-wide gene expression; the majority of genes ... Circadian orchestration of gene expression in cyanobacteria Genes Dev 1995, 9:1469-1478 Katayama M, Tsinoremas NF, Kondo T, Golden SS: cpmA, a gene involved in an output pathway of the cyanobacterial...

Ngày tải lên: 09/08/2014, 20:20

... activation of genes required for melanogenesis (tyrosinase, TYRP1, and TYRP2), survival, as well as the differentiation of melanocytes [18] The process of melanogenesis constitutes a complex series of ... antimelanogenic mechanism of Cpc The expression of tyrosinase and the production of melanin were determined to examine the antimelanogenic effect of Cpc The levels of signaling molecules such ... the abundance of cAMP and activated ERK1/2, which promoted the degradation of MITF, leading to the Page of 11 suppression of melanogenesis Moreover, Cpc attenuated the activation of p38 MAPK and...

Ngày tải lên: 10/08/2014, 10:20

... collectively highlight the importance of examining gene expression profiles (or related protein expression levels) over time The limits of gene expression profiling as a technique, albeit a very ... measure of the overall similarity of the dynamic behaviour of these samples The greatest temporal changes in gene expression for cord and adult monocytes noted above after 45 of LPS expo- Page of ... accumulation [29] Thus, gene expression profiling should be complemented with other methods in order to maximize there potential In the final analysis, the utility of gene expression profiling will be...

Ngày tải lên: 11/08/2014, 08:21

... their expression profiles in PUSABGD72 The expression profile of individual gene in the cluster is denoted by grey line and the mean expression profile is depicted by pink line The number of genes ... Average expression intensity of the cluster genes was much higher than that of the cluster genes and there was uniformity in the expression of the cluster genes Two ESTs [FL512394 and FL518992] of ... http://www.biomedcentral.com/1471-2229/10/24 Page of 14 Figure Hierarchical clustering analysis of 53 selected genes based on their gene expression patterns Analysis of expression profiles of 53 ESTs (Additional File...

Ngày tải lên: 12/08/2014, 03:21

... Discussion Synthesis of 3′-coterminal sgRNAs is one of the genome expression strategies adapted by members of the family Page of 14 Closteroviridae Evidence gathered with viruses in the genera Closterovirus ... the lack of evolutionary conservation in the regulation of gene transcription among monopartite members of the family Closteroviridae The 5′NTR of GLRaV-3 has an unusually long 5′NTR of 737 nt ... used to amplify gene- specific fragments for preparing non-radioactive riboprobes, Table S6: List of primer combinations used to generate gene- specific riboprobes, Table S7: List of gene- specific...

Ngày tải lên: 12/08/2014, 04:20

... viral gene expression, replication, and interaction with the host immune system, eventually leading to remarkable functional consequences [14,15] Transcriptional activation of HIV-1 gene expression ... degree of conservation was observed in all of them but in NFκB and Sp1 sites The HIV-1 promoter contains Sp1 and NF-kB binding sites that regulate gene expression via the recruitment of both ... delicate mechanism of regulation of viral gene expression by compensating any losses or gains in transcription levels This mechanism may play a key role in the adaptation process of recombinant variants...

Ngày tải lên: 12/08/2014, 04:21