... performed in a volume of 25 µl, consisting of 2.5 µl of 10 × PCR buffer, 2.0–2.5 µl of MgCl2 (25 mM), 0.5 µl of dNTPs (10 mM each; Promega), 0.5 µl of Hot Star Taq (5 U/µl), 0.5 µl of primer mix (20 ... subcutaneous injections of 100 µg of mBSA (Sigma, Deisenhofen, Germany) in 50 µl of saline solution, emulsified in 50 µl of complete Freund's adjuvant (Sigma), adjusted to a concentration of mg/ml heat-killed ... expression during the course of AIA Our observation concurs with that of van Meurs and colleagues [12] who noted a fast upregulation of mRNA for stromelysin during acute flare-up of murine AIA...
... variability of baseline values, there are very slight differences in Zrs at the end of the experiment between control and treated group Figure shows the time courses of Raw and of the estimates of the ... sustained condition of interstitial edema is a cause of progressive loss of integrity of the interstitial matrix [32] The obvious question then is: what is the mechanical resistance of the matrix macromolecules ... allowed the simultaneous control of piston position and valves and the acquisition of the data from the transducers (see below) This software permits the generation of three different flow waveforms...
... limited because of the poor availability of tissues at different stages in cancer development and because of the great diversity of genetic backgrounds among individuals [3-5] Mouse models of cancer ... expression profile distinct from that seen in mammary tumors induced by MMTV-Neu Comparison of expression profiles of tumors from several transgenic models has led to the identification of expression ... genes, we compared the expression profiles of a set of three fat samples with those of the 35 mammary tumors from MMTV-Wnt-1 and MMTV-Neu transgenic mice Expression of 741 genes was at least three-fold...
... expression of hypoxia-inducible factor (HIF)-1a in articular chondrocytes: involvement of HIF1a in the pathogenesis ofosteoarthritis Arthritis Res Ther 2005, 7:R904-R914 Silver IA: Measurement of pH ... that HIF-1 is of pivotal importance in cartilage development and homeostasis Furthermore, given the decreased oxygen levels and presence of inflammatory mediators during the course of OA, a causal ... analyzed the role of HIF-1 in chondrocytic energy generation, matrix synthesis and cell survival During hypoxic and normoxic conditions, functional inactivation of HIF-1α in the presence of interleukin-1β...
... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an embryonic cartilaginous limb) of the development of neural crest ... abnormalities called campomelic dysplasia [51,52] The effect of a complete loss of Sox9 during chondrogenesis was analyzed using a model of mice chimeras injected with homozygous embryonic Sox9)...
... to suppression of respiration during hypoxia In the absence of differential regulation of a specific nuclear gene coding for the subunits of CytOX, changes in the microenvironment of the cell may ... alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes CYC1 and ... results suggest that changes in mitochondrial gene expression precede changes in the expression of nuclear genome coded CytOX subunits during hypoxia We also tested the level of mRNA for subunit...
... starch content of the plantlets was in the callus of clone M41 However, because of a strong increase of dry matter in the callus, the amount of soluble carbohydrates in the shoots of clone M41 ... survival of rooted shoots The number of mature leaves at trans- planting seems to be an important morphological criterion in the survival of rooted shoots of hybrid walnut clones The number of mature ... concentration of 20 g·l sucrose) -1 generally low (fig 4) except concentration in the development medium, the plantlets of clone M41, with more than roots, had a survival rate of 94%, whereas only 63% of...
... within a margin of accuracy of ± 20 cc Page of (page number not for citation purposes) Radiation Oncology 2009, 4:26 http://www.ro-journal.com/content/4/1/26 in the range of to 699 ml of urine volume ... accuracy of the bladder scanner It is likely that patient attention to detail (i.e bladder emptying) accounted for the changesduring the first three weeks of treatment As such, it is Page of (page ... variations during radiation treatment of prostate cancer: Can the use of a bladder ultrasound scanner and biofeedback optimize bladder filling? Int J Radiat Oncol Biol Phys 2006, 65:371-377 Page of (page...
... groups of aggregates, as shown in the insert of Figure 8A Consistent with its mRNA profile, the numbers of IL-1b+ cells increased with time in HFC-fed animals (Figure 8B) indicating the relevance of ... http://www.journal-inflammation.com/content/8/1/8 A Page 12 of 14 B Figure Increased number of IL-1b+ cells in the liver of HFC-fed mice Immunohistochemistry of anti-IL-1b demonstrated increased number of IL-1b+ cells in HFC-fed ... tissues and liver of C57BL/6 mice These tables contain the gene expression profile of all genes in this study Table S2 The gene expression profile in epididymal adipose tissues of HFC-fed mice...
... groups of aggregates, as shown in the insert of Figure 8A Consistent with its mRNA profile, the numbers of IL-1b+ cells increased with time in HFC-fed animals (Figure 8B) indicating the relevance of ... http://www.journal-inflammation.com/content/8/1/8 A Page 12 of 14 B Figure Increased number of IL-1b+ cells in the liver of HFC-fed mice Immunohistochemistry of anti-IL-1b demonstrated increased number of IL-1b+ cells in HFC-fed ... tissues and liver of C57BL/6 mice These tables contain the gene expression profile of all genes in this study Table S2 The gene expression profile in epididymal adipose tissues of HFC-fed mice...
... investigate the effect of MP during environmental enrichment without the additional handling, however, due to the group housing and irregular drinking habits of gerbils administration of MP through the ... of data GT participated in the design of the study, and the drafting and revision of the manuscript Page of (page number not for citation purposes) Journal of Negative Results in BioMedicine 2008, ... JL, Galea LA: High post-partum levels of corticosterone given to dams influence postnatal hippocampal cell proliferation and behavior of offspring: A model of post-partum stress and possible depression...
... lymphocytic leukemia (CLL) Melanoma 16 of the patients were non-smokers with a high proportion of 75% (12 out of 16) of patients showing an oropharyngeal cancer 14 of those also were no habitual drinkers ... drinkers Sequence analysis of exon 10 of the CHK2 gene was performed for all patients However, none of the patients showed the presence of the 1100delC variant Discussion None of the investigated 91 ... Journal of Negative Results in BioMedicine 2010, 9:10 http://www.jnrbm.com/content/9/1/10 other than 1100delC in SCCHN as well as the definition of the role of CHK2 in the carcinogenesis of SCCHN...
... systems: • Advancement of computer hardware and software • Computing power, memory and communications • Development of a vision of integrated information systems • Reengineering of companies to shift ... Edition 37 ERP System Costs • The cost of an ERP system: • Depends on the size and complexity of the software package, which is a function of the size of the firm • Includes new hardware required ... led to the development of Enterprise Resource Planning (ERP) systems • Describe the distinguishing modular characteristics of ERP software • Discuss the pros and cons of implementing an ERP system...
... management ofosteoarthritisof the hip and knee: 2000 update American College of Rheumatology Subcommittee on Osteoarthritis Guidelines Arth Rheum 2000; 43:1905-1915 Haq I, Murphy E, Dacre J Osteoarthritis ... trial of subjects with OA, taking a single oral daily dose of 40 mg UC-II on an empty stomach prior to bedtime for 42 consecutive days, an average of 26% reduction of pain was noted in four of five ... measures and blood measures of OA of the knee compared to G+C It was hypothesized that UC-II would reduce symptoms of OA of the knee to a greater extent than G+C A meta-analysis of 20 randomized control...
... example of growth of forestry exports in Chile which was facilitated by the availability of forestry engineering graduates from local universities There is less evidence of the effects of vocational ... technology Offshoring of services from developed to developing countries (and from developing to other developing) has now taken off While offshoring started in low value-added activities (back-office ... US; 30–50% of IT workers and 60% of physicians have emigrated • South Africa has lost 4600 professionals every year (0.3% of national stock) (Bhorat et al., 2002); 10000 health professionals...
... diversity of products of the bank will attract more customers • Scale of bank activities: the size of capital affects the development and expansion of the bank, including consumer loan professional ... close relationship with each other under the control of the Board of Directors Being aware of the important role of THQV in the whole system of Techcombank, managers and staff at THQV continually ... efforts of outstanding loans as well as mobilization of capital growth and profit before tax of the average growth in recent years • Customers: In 2012, Techcombank will become increasingly on professional...
... activated sludge reactor was lyophilized After the addition of 2mL of chloroform and acidified methanol (10% sulfuric acid with around 100mg/L of benzoic acid), the content in the tube was mixed well, ... rate of 30mL/minute Sodium 3-hydroxybutyrate (Tokyo Chemical Industry, Japan) was used as the standard for 3-hydroxybutyrate (3HB) unit in PHA The copolymer composed of 81% of 3HB and 19% of 3-hydroxyvalerate ... Journal of Water and Environment Technology, Vol 6, No.2, 2008 Technology, China The concentration of PHA in these reactors was determined, and the fractions of carbon stored as PHA...