2 culture systems for stem cell expansion

... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, ... 2007) Lymphoid stem cells, Myeloid stem cells Unipotent Retain self-renewal capacity but can only produce one cell type (Sorgner, 2007) Muscle stem cells Apart from the plasticity, stem cells can ... pluripotent cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells...

Ngày tải lên: 11/09/2015, 10:00

... predict behavior of stem cells in vivo Overall, microfluidics tools can be used for spatio­ temporal control over the stem- cell microenvironment, so that the ideal ex vivo niche for cell survival and ... [3] For cell biologists, the major impact has been in cell separation, single -cell resolution of the dynamics of gene expression, and insights into how mechanical forces applied to individual cells ... major advantage of microfluidics-based cell- culture systems, and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) are particularly sensitive...

Ngày tải lên: 06/08/2014, 19:21

... models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; the stem- cell pool ... of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are not inherent, cell- autonomous properties, ... Lophotrochozoa, and Ecdysozoa) Whether their similar stem- cell systems are homologous or convergent remains an open question The biology of these stem cells is currently being investigated in the planarians...

Ngày tải lên: 06/08/2014, 19:21

... extremely narrow cell- type specificity Since appropriate genetic circuitry models are likely to depend upon cell- specific, or at least tissue-specific, data, new technologies for obtaining highly ... Responses were further characterized by quantitative RT-PCR for four of the genes activated individually by brassinosteroid and auxin; for these genes, the two hormones appeared to act synergistically ... responses deposited research Figure Auxin and brassinosteroid regulate transcription mediating cell expansion by both independent and interconnected mechanisms Only components known to act in transcriptional...

Ngày tải lên: 14/08/2014, 14:21

... approach therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, Weissman addressed this intriguing ... and characterize the cancer stem cell in order to eliminate it It is an exhilarating time for stem- cell research The work presented at the meeting suggested that stem- cell research will lead to ... originate from the transformation of normal stem cells Work in Weissman’s laboratory has established the role of a leukemia stem cell in leukemia If the results hold true for other tumors, the...

Ngày tải lên: 14/08/2014, 14:21

... 1.3.6 Cells 29 1.3.6.1 Immune Cells 29 1.3.6.2 Epithelial Cells 30 1.4 Adult Stem Cells 31 1.4.1 Mammary Stem Cells 32 1.4.1.1 Evidence for ... reference from other systems with epithelial cells’ lining, studies have document the presence of the of the stem cell in the luminal discharges due to the proximity of the stem cell niche to their ... Staining for neural stem/ progenitor markers 82 Figure 3-13: RT-PCR for epithelial cell markers 83 Figure 3-14: Staining for epithelial cell markers 84 Figure 3-15: Staining for...

Ngày tải lên: 11/09/2015, 09:57

... Injectable deliverey systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 2.4.4 ... fabrication and cell seeding 19 2.4 Injectable delivery systems for stem cell therapy Two kinds of approaches are primarily under consideration for stem cell therapies – (i) injection of stem cells with ... various types of stem cells for such stem cell based therapies, few of which are discussed in the following sections Stem cells are broadly classified into embryonic and adult stem cells based on...

Ngày tải lên: 09/09/2015, 11:18

... relating to cell- free translation systems for enhancing the synthesis of biologically active proteins, the creation of cell- like compartments and the synthesis of artificial proteins Overview Cell- free ... artificial proteins Overview Cell- free translation systems are based on the cellular ribosomal protein synthesis system Generally, the system is composed of a cell extract (referred to as the S30 fraction) ... S30-directed, cell- free translation systems has improved greatly over the last two decades In 1988, the continuous-flow cell- free system [3] represented the first demonstration that cell- free translation...

Ngày tải lên: 19/02/2014, 06:20

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... stem cell lines, so the ability to share cell lines will create greater access for qualified scientists to participate in human stem cell research A uniform set of criteria for deriving hES cell...

Ngày tải lên: 06/03/2014, 15:20

... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral ... positive in both wild-type cells and the two Ptb) ⁄ ) ES cell clones Scale bar: 100 lm (B) Cell proliferation assay Cells (5 · 104) were seeded (d0) and counted every day for days of culture The...

Ngày tải lên: 07/03/2014, 00:20

... the ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, http:// www.appliedbiosystems.com) and the specific primerpairs for BDNF (forward: 5′-ACC CTG AGT TCC ACC AGG TG-3′, reverse: ... neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant for four ... b-actin (forward: 5′-CTA CAA TGA GCT GCG TGT GG-3′, reverse:5′-CAG TCA GGA TCT TCA TGA GG-3′) The thermo-profile was 50°C for and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for...

Ngày tải lên: 18/06/2014, 16:20

... No (%) Positive for JE antigen No (%) Positive for WN antigen No (%) Positive for Den-2 antigen No (%) Total No (%) Negative Positive for JE virus Positive for WN virus Positive for Den-2 virus ... mechanical force enhances the viral entry in to the cells, which in turn reduces the total time taken for the virus to produce infection of cells [11] Originally the SVC was described for murine ... districts of Tamil Nadu State Cell line Porcine Kidney (PS) cells (supply No 3109 A) obtained from National Center for Cell Sciences, Pune, were used in the study Cells were grown in plastic tissue...

Ngày tải lên: 19/06/2014, 08:20

... IGC 28 Expert Systems for Human, Materials and Automation Table Radius parameters, mean incidence and parametric index for excipients DC 29 SeDeM Diagram: A New Expert System for the Formulation ... reinforcement learning; A rough set approach", Proc 5th Int Conf Hybrid Intelligent Systems, pp.187-192, 2005 F Radermacher, "Decision support systems: Scope and potential", Decision Support Systems, ... 1986 1987 1988 1992 # of expert systems developed 50 86 1100 2200 12000 Table Increase in number of expert systems developed yearly (based on Durkin, 1998) Area systems Engineering & manufacturing...

Ngày tải lên: 19/06/2014, 10:20

... No (%) Positive for JE antigen No (%) Positive for WN antigen No (%) Positive for Den-2 antigen No (%) Total No (%) Negative Positive for JE virus Positive for WN virus Positive for Den-2 virus ... mechanical force enhances the viral entry in to the cells, which in turn reduces the total time taken for the virus to produce infection of cells [11] Originally the SVC was described for murine ... districts of Tamil Nadu State Cell line Porcine Kidney (PS) cells (supply No 3109 A) obtained from National Center for Cell Sciences, Pune, were used in the study Cells were grown in plastic tissue...

Ngày tải lên: 20/06/2014, 04:20

... to the cell culture medium, the suspension cells started to adhere and form neuronal networks (Figure 3c) Mesenchymal stem cells (MSCs) were used as a model for a sensitive in vitro system [43] ... biological cells on an individual level in a small defined cell population Biological microelectromechanical systems (Bio-MEMS) present a suitable approach for analyzing a small amount of cells on ... compared to single -cell analysis, since about 200 to 250 cells are present in each cavity and thus a small monolayer can be formed To observe all cells of a cell layer in conventional cell culture...

Ngày tải lên: 21/06/2014, 00:20

... of cultured stem cells For abbreviations of stem cells, see Table 66-1 Note that stem cells are often abbreviated with or without "cells," e.g., ES cells or ESCs for embryonic stem cells m, mouse; ... including germ cells Multipotent cells (e.g., HS cells) can form multiple cell lineages but cannot form all of the body's cell lineages Oligopotent cells (e.g., NS cells) can form more than one cell lineage ... progenitor cells) that can divide into different cell types but lack self-renewing capacity Unipotent cells or monopotent cells, e.g., spermatogonial stem (SS) cells, can form a single differentiated cell...

Ngày tải lên: 07/07/2014, 01:20

... transplantation of a variety of different cell types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose stem cells Bone marrow stem cells in particular have been examined ... Figure 67-1 Strategies for transplantation of stem cells Undifferentiated or partially differentiated stem cells may be injected directly in the target organ or intravenously Stem cells may be differentiated ... might provide an ideal source of stem cells for therapeutic use For effective myocardial repair, cells must be delivered either systemically or locally, and the cells must survive, engraft, and...

Ngày tải lên: 07/07/2014, 01:20

... more uniform size Environmental Protection Agency, "Control Techniques for Particulate Emissions from Stationary Sources" gives characteristics of refuse derived fuels 3-3 Fuel burning systems ... prepared for combustion by use of mechanical atomizing burners or twin oil burners In order for fuel oil to be properly atomized for combustion, it must meet the burner manufacturer's requirements for ... Regulations for coal, fuel oil, and natural gas The above allowable emission rates shown are for boilers with a heat input of 250 million British thermal units (MMBtu) and above 3-5 Formation of...

Ngày tải lên: 06/08/2014, 01:21

... without stem cell support Competing interests None declared References 10 11 12 13 14 Marmont AM, van Lint MT, Gualandi F, Bacigalupo A: Autologous marrow stem cell transplantation for severe systemic ... with the same dose of cyclophosphamide without hematopoietic stem cell support, and also to hasten stem cell regeneration of the immune system under the umbrella of the ATG effect Based on the same ... hematopoietic stem cells are not necessary for re-engraftment While intensely immune suppressive, the regimen is not myeloablative and endogenous hematopoiesis would recover The hematopoietic stem cells...

Ngày tải lên: 09/08/2014, 01:23

... hematopoietic stem cell transplantation Hematopoietic stem cell transplantation (HSCT) is a complex multistep procedure involving mobilisation and harvesting of hematopoietic stem cells (HSCs) ... without stem cell rescue has been tested in SLE and scleroderma patients [37-39] The rationale for that procedure is a rapid eradication of autoreactive effector cells while sparing stem cells ... European Group for Blood and Marrow Transplantation, et al.: Autologous stem cell transplantation for systemic lupus erythematosus Lupus 2004, 13:168-176 Saccardi R, Kozak T, Bocelli-Tyndall C,...

Ngày tải lên: 09/08/2014, 10:23