2  bind to a function

... and Metadata) (MSIL and Metadata) Managed Module Managed Module (MSIL and Metadata) (MSIL and Metadata) Assembly Assembly Lead-in The common language runtime uses an assembly as the functional unit ... Introduction to a Managed Execution Environment Defining a Namespace and a Class Topic Objective To describe how to define namespaces and classes in C# Lead-in C# supports the creation of custom namespaces ... common language runtime uses an assembly as the functional unit of sharing and reuse Definition of an Assembly An assembly is a unit of class deployment, analogous to a logical dll Each assembly...

Ngày tải lên: 09/12/2013, 17:15

... DNA-binding site A 60 bp single-stranded DNA RDM10, with 10 randomized oligonucleotides in the center, i.e CTGTCAGTGAT GCATATGAACGAATN10AATCAACGACATTAGGATC CTTAGC was synthesized A 100 ng sample ... of a three-stranded antiparallel b-sheet and an a- helix, packed approximately parallel to the b-sheet, with the seven thoroughly conserved amino acids (Arg6, Arg8, Trp10, Glu16, Arg18, Arg26 and ... expression Plant Mol Biol 24, 701–713 Prabakaran P, An J, Gromiha M, Selvaraj S, Uedaira H, Kono H & Sarai A (2001) Thermodynamic database for protein-nucleic acid interactions (ProNIT) Bioinformatics...

Ngày tải lên: 18/02/2014, 13:20

... endo-acting into an exo-acting a- amylase such as the natural maltotetraose-forming exo-amylase [10] or B stearothermophilus maltogenic a- amylase [23] Met53 was indicated in the modelled AMY2/maltodecaose ... inhibitory substrate analogues derived from acarbose and barley a- amylase (AMY2 [16]); and Taka-amylase A (TAA [17]) (A) Stereo view of interactions involving segments of ba loops and (i.e domain ... from AMY2 (in green) and TAA (in black) The superimpositioning was guided by the catalytic acids (D179AMY2, E204AMY2, and D289AMY2 and D206TAA, E230TAA, and D297TAA) The invariant Y51AMY2 and...

Ngày tải lên: 31/03/2014, 08:20

... cleavage provide additional evidence in favor of a mechanism for signaling Bax to the mitochondria that is either parallel to or independent of death receptor and/or caspase activation A similar ... receptor (data not shown), and inhibition of caspase activation had no effect on the mitochondrial translocation of Bax In addition, the relative lack of caspase activation and absence of downstream ... significantly change the Bax:Bcl-2 ratio (Fig 2A) , analysis of subcellular fractions clearly showed translocation of Bax from the cytosol to the mitochondria (Fig 2B) Mitochondrial localization of Bax...

Ngày tải lên: 16/09/2015, 17:11

... thermochemical database 66 gauge problem in calculation of magnetic properties 200 Gaussian-type-orbitals (GTO) 98 generalized gradient approximation (GGA) 75 ff – meta GGA 90 gauge-invariant/including atomic ... for example, the average distance between the electrons is too small at the Hartree-Fock level, this automatically will lead to a kinetic energy that is too large and a nuclear-electron attraction ... is not acceptable as a model wave function for fermions because it assigns a particular one-electron function to a particular electron (for example χ1 to x1) and hence violates the fact that r...

Ngày tải lên: 21/08/2013, 10:29

... SqlConnection("server=localhost;database=Northwind;uid=sa;pwd=sa"); // monitor the StateChange event using the StateChangeHandler() method mySqlConnection.StateChange += new StateChangeEventHandler(StateChangeHandler); ... example defines a method named StateChangeHandler to handle the StateChange event You'll notice that the second parameter to this method is a StateChangeEventArgs object You get the original ... state of the connection to the database The State property returns a constant from the ConnectionState enumeration Note An enumeration is a list of numeric constants, each of which has a name...

Ngày tải lên: 14/12/2013, 13:15

... serial or COM1 It may or may not be identified Step Locate the RJ-45 to DB-9 adapter One side of the adapter connects to the PCs serial interface and the other to the RJ-45 rollover cable connector ... connectors a Examine the router or switch and locate the RJ-45 connector labeled “Console” Step Identify the computer serial interface, which is COM or a It should be a or 25-pin male connector labeled ... serial interface on the PC or dumb terminal is a DB-25, an RJ-45 to DB-25 adapter will be needed Both of these adapters typically come with a Cisco router or switch Step Locate or build a rollover...

Ngày tải lên: 21/12/2013, 19:15

... N-terminal trunk binds into the nonprimed Table Average distances between CA atoms of the stefins and catalytic residues of cysteine proteases Distance calculated ˚ d (A) Papain–stefin B Cathepsin ... (cathepsin C): exclusion domain added to an endopeptidase framework creates the machine for activation of granular serine proteases EMBO J 20, 6570–6582 18 Molgaard A, Arnau J, Lauritzen C, Larsen ... structures are almost 10 A apart It is concluded is that the occluding loop is rather flexible and will adapt to structural features of the inhibitors as well as to the packing constraints of the...

Ngày tải lên: 18/02/2014, 04:20

... of ethanol and treatment with disulfiram Alcohol Alcohol 28, 461–468 30 Isse T, Oyama T, Kitagawa K, Matsuno K, Matsumoto A, Yoshida A, Nakayama K, Nakayama K & Kawamoto T (2002) Diminished alcohol ... Roman J, Gimenez A, Lluis JM, Gasso M, Rubio M, Caballeria J, Pares A, Rodes J & Fernandez-Checa JC (2000) Enhanced DNA binding and activation of transcription factors NF-kappa B and AP-1 by acetaldehyde ... (Fullerton, CA) PAR metal transfer assay Metallochromic indicators provide a rapid means of investigating metal–protein equilibria [58,59] PAR is such an indicator Binding of zinc ions changes its absorbance...

Ngày tải lên: 19/02/2014, 06:20

... the main int dollar, quarterR, dimeR, nickelR, pennyR, remainder; Variables that are used in the main are declared There are six variables of type integer These variable names (identifiers) stand ... dollars % 10 calculate dime rolls and remainder dime_rolls = remainder / remainder = remainder % calculate nickel rolls and remainder nickel_rolls = remainder / remainder = remainder % calculate ... the program line by line int main() Every program must have a main function A program begins executing with the main function Main returns an integer value to DOS { The left bracket indicates the...

Ngày tải lên: 20/02/2014, 08:20

... X-ray structural analysis of a prokaryotic [5] and an eukaryotic [17] cyt b6f complex: both in the case of the cyanobacterial complex (Mastigocladus laminosus) and the green algal complex (Chlamydomonas ... respectively, as well as a sharp negative feature at 555 nm and positive features near 548 and 530 nm These data indicate 584 Fig Comparison of absorbance and LD spectrum The absorbance spectrum ... linear gradients, with increasing hydrophobicity (increased ethylacetate percentage: fi 20%, 20 fi 50%, 50 fi 100%), at room temperature and at an average flow rate of 0.7 mLÆmin)1 (A) Acetone extract...

Ngày tải lên: 07/03/2014, 16:20

... shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candida glabrata; CIONA, Ciona intestinalis; ... of human DIDO isoforms and were searched against PFAM [22,23] and SMART [24] databases to automatically detect domains; they were further used as queries against NCBI databases using PSIBLAST [25,26] ... suggest that DIDO-related apoptosis occurs as a result of alterations in DNA regulation caused by chromatin instability Computational analyses Although all splice variants share common domains, long...

Ngày tải lên: 07/03/2014, 21:20

... cPLA2 -a at its site of localization Several studies have also implied that cPLA2 -a may be regulated by cytoskeletal interactions Cytochalasin B, an inhibitor of actin polymerization, was shown to ... cytosolic phospholipase A2 -alpha to EA.hy.926 endothelial cell membranes leads to calcium-independent association Eur J Biochem 271, 69–77 Naraba H, Murakami M, Matsumoto H, Shimbara S, Ueno A, ... cPLA2 -a and convert arachidonic acid into prostaglandin H2 [8,9] To date, two distinct COX isoforms, COX-1 and COX-2, have been identified and characterized, and an alternative splice variant...

Ngày tải lên: 16/03/2014, 18:20

... a biotin head at the 5¢-end HepG2 nuclear extracts were incubated with avidin–agarose beads (Sigma) at °C for 30 to eliminate materials in the extracts that bind nonspecifically to the beads After ... 5047–5052 Hoff, C.M & Jacob, S.T (1993) Characterization of the factor E1BF from a rat hepatoma that modulates ribosomal RNA gene transcription and its relationship to the human Ku autoantigen Biochem ... possesses transcriptional regulatory activity and that a variety of nuclear transcription factors interact specifically with MREa [11,33,38,39] These findings suggest that transcriptional activator proteins...

Ngày tải lên: 17/03/2014, 23:20

... standard curve, and normalized to the housekeeping gene, hypoxanthine-guanine phosphoribosyltransferase Statistical analysis All data are expressed as the means ± standard deviations or standard errors ... characterization of hSRP1 gamma, a tissue-specific nuclear transport factor Proc Natl Acad Sci USA 95, 582–587 10 Kamei Y, Yuba S, Nakayama T & Yoneda Y (1999) Three distinct classes of the alpha-subunit ... nuclear pore-targeting complex (importin-alpha) are differentially expressed in adult mouse tissues J Histochem Cytochem 47, 363–372 11 Yasuhara N, Shibazaki N, Tanaka S, Nagai M, Kamikawa Y,...

Ngày tải lên: 28/03/2014, 23:20

... TGTAAAG TGTAAAG + ) + )95 )1100 )1470 ACACacG ACACttG ACACaaG + + + + ) ) ) ) ) )140 )1100 )1596 )1632 )140 )1100 )1596 )1632 )1874 CAaaTG CAagTG CAaaTG CAaaTG CAaaTG CActTG CAttTG CAgtTG TGAGTCA ... peptide was constructed as follows The DNA fragment was amplified from GmPDIM cDNA by PCR using the primers 5¢-GACGACGACAAGATGC ACGCACTCTATGGAGC-3¢ and 5¢-GAGGAGAAGC CCGGTTCATAGCTCATCCTTGCTTGAAG-3¢ ... CCAAT-N9-CCACG )300CORE TGTAAAG DPBFcore Dc3 ACACNNG E-box CANNTG GCN4 motif TGAGTCA Prolamine box TGCAAAG RY repeat CATGCA SEF3 motif AACCCA SEF motif RTTTTTR a Distance from ATG Sequencea Function...

Ngày tải lên: 30/03/2014, 04:20

... Esposito and I Caputo 132 Akagi A, Tajima S, Ishibashi A, Matsubara Y, Takehana M, Kobayashi S & Yamaguchi, N (2002) Type XVI collagen is expressed in factor XIIIa+ monocyte-derived dermal dendrocytes ... the art Minerva Biotec 14, 121–128 137 Nishiura H, Shibuya Y, Matsubara S, Tanase S, Kambara T & Yamamoto T (1996) Monocyte chemotactic factor in rheumatoid arthritis synovial tissue Probably a ... b-sandwich, a core (which contains a transamidation site and a Ca2+-binding site, and has a helices and b sheets in equal amounts), and two C-terminal b-barrel domains It has been suggested that glutamyl...

Ngày tải lên: 30/03/2014, 15:20

... (1991) A contrastive evaluation of functional unification grammar for surface language generation: A case study in choice of connectives In C Paris, W Swartout, ~c W Mann (Eds.), Natural Language ... collect a set of features which are used to select a TAG tree that realizes the head and into which the arguments can be fit The traversal is also responas a classification of all f m l c t i o n a ... single rank establishes a set of functional choices that can be used to select a TAG tree The selection process in any TAG-based generator can be considered as providing a classification of TAG trees...

Ngày tải lên: 31/03/2014, 06:20

... therapies, all off-site whole blood specimens are shipped to a central laboratory according to a standard operating protocol, and monitored strictly for quality control and quality assurance ... different stages of apoptosis was evaluated for CD4 and CD8 T cell populations (A) Percentage of CD4 lymphocytes in early stages of apoptosis (Annexin V+, 7AAD-) and (B) late stages of apoptosis (Annexin ... were thawed and rested overnight at 37°C before staining with CD4, CD8, Annexin V and 7-AAD The viable populations were defined as Annexin V negative and 7AAD negative and are expressed as a percentage...

Ngày tải lên: 18/06/2014, 19:20