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... for the specific MAP2 binding resides within the 32 residues (amino acids 456–487) of the v-KIND KIND2 domain To examine whether the MAP2 binding core site of v-KIND binds to intact MAP2 protein, ... in the central domain of MAP2 contain the v-KIND binding core module MAP2 consists of three main structural domains: the cAMP-dependent protein kinase regulatory subunit RII binding domain, the ... v-KIND KIND2 domain contain the MAP2-binding core site (A) Division of the KIND2 domain (456–620) into five subregions: KIND2-1 (amino acids 456–487), KIND2-2 (amino acids 456–521), KIND2-3 (amino...

Ngày tải lên: 14/02/2014, 19:20

... analysis in murine monocytic RAW 264.7 cells The intensity of the ECL signal was determined using the alphaease fc sofware and divided by the protein concentration of the sample ESE-1 protein was ... To further our understanding of ESE-1 function during in ammatory processes, we have now explored the involvement of ESE-1 in the regulation of another in ammation-related gene, COX-2, in monocytic ... specifically retaining the COX-2 promoter region spanning all five Ets sites shown in Fig ()186 to +56 of the transcription start site) was only obtained in cells transfected with the plasmid containing ESE-1...

Ngày tải lên: 19/02/2014, 17:20

... animals kept in the aquarium are lacking a factor that causes either the expression of the gene encoding the (2–5)A synthetase or the activation of the enzyme One potential factor could be the differential ... in the Northern blotting experiment The results show that in the absence of the heat-killed bacteria no transcripts, corresponding to 1.4 kb (2–5)A synthetase mRNA, can be identified in the Northern ... activation of the enzyme resulting in the formation of the p3An products and a second, that increases the steady-state level of the transcripts of the corresponding (2–5)A synthetase gene In vertebrate...

Ngày tải lên: 21/02/2014, 15:20

... Internet for finding information about goods and services (ranging from 12% in Romania to 79% in the Netherlands) 33% use the Internet for seeking health-related information (ranging from 10% in Bulgaria ... 56% in Finland); and 31% use the Internet for reading online newspapers and magazines (ranging from 18% in Poland to 64% in Denmark and Finland) (Eurostat 2009 data) The usefulness of the Internet ... widely to support learning in an informal way, formal Education and Training is lagging behind in reaping the benefits of ICT to increase and improve learning opportunities The Rise of Social Media...

Ngày tải lên: 21/02/2014, 21:20

... of intracellular ROS [14] On the other hand, studies in cells overexpressing UCP2 have reinforced the belief that UCP2 plays a role in limiting intracellular ROS production, as has been shown in ... hypothesis [29] that an increase in the mitochondrial membrane potential would slow the transport of electrons through the respiratory chain, increasing the time of interaction between these electrons ... uncoupling) In the present study we use the immunomodulators to reveal the mechanism of action of toxic MDPs on mitochondrial respiration by correlating the uncoupling effect induced by these...

Ngày tải lên: 05/03/2014, 23:20

... showed the presence of CMP and AMP (from FAD) GMP was not present in any Qor extract, indicating that the host strains did not incorporate Mo-MGD, or free GMP, into the cofactor binding domain of the ... formed during the preparation process in varying amounts [24] The finding that in each of the three Qor enzymes the majority of the Mo(V) species was represented by the rapid type EPR signal indicates ... Huttermann, J & Fetzner, S (2002) The molybdenum¨ containing hydroxylases of quinoline, isoquinoline, and quinaldine In Molybdenum and Tungsten Their Roles in Biological Processes (Sigel, A &...

Ngày tải lên: 17/03/2014, 10:20

... incubated without the RS or Grx2; Grx2-incubated, samples incubated in the presence of the RS plus Grx2 at the indicated concentrations Anti-FLAG, loading control performed as described in Experimental ... cysteines were determined to be present on the surface, with some of them being totally exposed and others slightly buried but still solventaccessible All of the other cysteine residues are either ... that the 4898 kDa peptide detected after 30 incubation that contains the conserved CXXC motif retains dethiolase activity Nevertheless, the central point addressed here is that Grx2 is involved in...

Ngày tải lên: 23/03/2014, 07:20

... hampered by introduction of an aromatic side chain in the middle of the AMY1 binding cleft which apparently disturbed crucial steps in the mechanism, perhaps involving contacts between domains A and ... of the global structure of the AMY2 (top) and TAA (bottom) complexes [16,17] The inhibitors are in green and the arrow indicates the nitrogen (in dark blue) that corresponds to the oxygen of the ... between subsite )2 and other parts of the binding cleft emphasized the intimate contact in between ba loop and domain B and its importance in activity [15] The activity towards insoluble Blue Starch...

Ngày tải lên: 31/03/2014, 08:20

... region, themselves very weakly interacting with Tax2, may participate in generating the correct conformation for the critical binding site of the strongly interacting 253-1130 CIITA fragment The ... localization unveiled the co-localization of Tax-2 and CIITA both in the cytoplasm and the nucleus, and the role of CIITA in redirecting, upon binding, Tax-2 molecules mostly in the cytoplasm These results ... of the CIITA molecule located at the 1-252 N-terminal and mostly at the 4101130 C-terminal site Interestingly, however, and in similarity to the results obtained in COS-7 cells [22], only the...

Ngày tải lên: 18/06/2014, 22:20

... box plots The box represents the interquartile range that contains 50% of the values The whiskers extend from the box to the highest and lowest values The line across the box indicates the median ... Protein (GFAP) was obtained from Monosan (clone 6F2, Uden, The Netherlands) Morphometric investigation was aimed at determining the area density occupied by the immunoreactive glial cells in the ... death [13] These in vitro findings indicate that the activation of microglia may play an important role in the expression of COX-2 and cell cycle proteins in neurons Post mortem as well as in vivo...

Ngày tải lên: 19/06/2014, 22:20

... are involved in the expression of the inflammatory mediators Particularly, the role of the phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) and glycogen synthase kinase-3 ... On the other hand, the Akt inhibitor X increased significantly the production of PGE2 and PGD2 in LPS-activated microglia (Fig 1C) To investigate whether the enhancement of PGE2 was due to an increase ... phosphatidylinositol 4,5-bisphosphate to generate phosphatidylinositol-3,4,5-trisphosphate The latter molecule binds to the pleckstrin homology domain of one of the Akt (also known as protein kinase...

Ngày tải lên: 19/06/2014, 22:20

... region, themselves very weakly interacting with Tax2, may participate in generating the correct conformation for the critical binding site of the strongly interacting 253-1130 CIITA fragment The ... localization unveiled the co-localization of Tax-2 and CIITA both in the cytoplasm and the nucleus, and the role of CIITA in redirecting, upon binding, Tax-2 molecules mostly in the cytoplasm These results ... of the CIITA molecule located at the 1-252 N-terminal and mostly at the 4101130 C-terminal site Interestingly, however, and in similarity to the results obtained in COS-7 cells [22], only the...

Ngày tải lên: 20/06/2014, 04:20

... first coordinate is the same as those of M (there is only one such point M because the point at the vertical in nity is a double point of the quartic) The image F(M) is the symmetric point of M ... We begin by identifying the fixed point Lemma 3.1 If a = b = 0, then sequence (1.2) tends to If a + b > 0, then the fixed point of the dynamical system (1.3) is the unique positive root of the equation ... Γ0 (K) Proof (1) We work here in R+ , and begin in the case when b + d > Suppose that there ∗ is a point (X,Y ) in the set {G ≤ K }, lying on the hyperbola XY = λ2 Then we have X + Y ≥ 2λ and...

Ngày tải lên: 23/06/2014, 00:20

... reaction in the cytoplasm of the PCV ORF1 gene inserted recombinant baculovirus infected insect cells The PCV ORF2 gene containing recombinant baculovirus infected insect cells showed intracytoplasmic ... recombinant virus infected insect cells Expression of the recombinant proteins from recombinant virus infected insect cells were confirmed by IFA test using polyclonal antiserum to PCV type Intracytoplasmic ... recombinant protein expressed in the baculovirus system did not show any noticeable changes in the molecular mass by tunicamycin treatment This may indicate that the ORF2 of the PCV has minor...

Ngày tải lên: 07/08/2014, 15:20

... setanegomoh ehT rezinegomoh a ni sekorts 02 htiw )nitpepuel lm/gµ 01 ,ninitorpa lm/gµ 01 ,FSMP Mm ,4OV3aN Mm ,04-p tedinoN %1.0 ,lCaN Mm 021 ,sirT Mm 04( reffub sisyl ni dezinegomoh saw evren ... yppolf depoleved seditpep 62PS htiw dezinummi star siweL ,feirb nI ]1[ troper suoiverp ruo ni nwohs saw NAE fo esruoc lacinilc ehT NAE fo noitavresbo lacinilC stluseR )napaJ ,supmylO( epocsorcim ... :segats lacinilc ruof otni dedivid saw NAE fo ssergorp ehT noitazinummi retfa dna syad ta )ASU ,amgiS( nixot sissutrep fo gn 05 htiw detaert saw tar hcaE ]1[ detroper ylsuoiverp sa ,yllacinilc detaulave...

Ngày tải lên: 07/08/2014, 18:21

... trenching The remaining fine root necromass was then compared to initial fine root biomass and necromass in soil cores Coarse roots (2-10 mm in diameter) excavated during the installation of the ... rapid during the first stage than during the second Our k value for fine roots, which was simply obtained by comparing the remaining fine root mass years after trenching to fine root mass in soil ... probe inserted in the soil to a depth of 10 cm in the vicinity of the soil respiration chamber Volumetric water content of the soil (&thetas; ) v was measured every 10 cm in depth on the main plot...

Ngày tải lên: 08/08/2014, 14:21

... penicillin/streptomycin using a humidified cultivator that maintained 37°C and 5% CO2 The normal cell line was cultivated using the same cultivator in Dulbecco's modified Eagle's medium (DMEM) combined ... Half were methylated in the MCF-7 cell line; MINT 1, MINT 31, p16 were methylated and MINT 2, p14, E-cadherin were unmethylated None were methylated in the CCD-112 CoN cell lines (Table 3) XTT ... compaction, chromatin can be divided into euchromatin and heterochromatin Euchromatin is at a relaxed state in which genes are actively undergoing transcription Heterochromatin contains inactivated...

Ngày tải lên: 09/08/2014, 04:21

... actual pain intensity = mean of 3-hour and 5-hour assessments bVAS worst pain intensity = worst pain intensity in the past 12 hours cVAS average pain intensity = average pain intensity in the past ... during the multiple-dose pain assessment phase Patients rated actual pain intensity, average pain intensity and worst pain intensity using a 100 mm VAS after activity in the morning and evening ... Blinding was maintained by the double-dummy technique Following days of study medication, patients had a final clinic visit, during which final pain assessments were made, pain records from the...

Ngày tải lên: 09/08/2014, 07:20

... sections were incubated for 30 minutes at room temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase ... The GAG content reflected proteoglycan biosynthesis and accumulation in the matrix, and under hypoxic conditions there was a 1.9-fold increase in the total GAG per aggregate In spite of the increased ... staining for 3G5, and negative staining for low-affinity nerve growth factor receptor (LNGFR), STRO-1, CD34 and CD56 No staining was observed for the IgG control The staining pattern was confirmed by...

Ngày tải lên: 09/08/2014, 10:20

... by the dotted horizontal line The red dots indicate the average expression in gene groups with increasing TFIIB occupancy They were determined by moving a sliding window (step size 0.1) over the ... resolution Binding of TBP and the initiating form of RNAPII (phosphorylated at serine in its carboxy-terminal domain) were monitored in parallel All three factors showed pronounced binding to the GAPDH ... lesser extent) NC2 binding, thereby providing a model for binding of GTFs in the absence of core elements and alterations in TFIIB/NC2 ratios inside cells In addition to defining a library of promoters...

Ngày tải lên: 09/08/2014, 20:21