1d spectra and the structural reporter region

... Perhaps the most impressive display of the technique was the targeting of the surface of interleukin-2 near to the known receptor-binding site Although the site did not seem druggable, Wells and ... nearly every structural problem has been studied using at least three techniques, or more.” This captured one of the themes of the meeting, that hybrid approaches are the order of the day, and indeed, ... catalysis, as identified by structural and biochemical experiments, for nearly 500 proteins They have also developed approaches to compare these sites, and the ligands that bind to proteins of...

Ngày tải lên: 14/08/2014, 17:22

... investigated the C-terminal region of the RmInt1 IEP up to the maturase domain and the C-tail We found that the C-tail and upstream amino acid residues, located in a predicted a-helical region, form ... which the Y354 and Y355 amino acids were replaced by alanine residues, and the 2.5· mutant [27], in which the IEP was truncated in the RT domain Therefore, the pattern of inhibition for the C-terminal ... b-strand in the C-tail The two conserved domain X motifs are found at or near the C-termini of a2 and a3, respectively The a-helices a1, a2 and a3 potentially correspond to a-helices aH, aI and...

Ngày tải lên: 15/03/2014, 09:20

... band very close to the LH1 a band, indicated by the arrow, is apparent in the PufX54* mutant (lane 6) This band was excised from the gel after SDS/PAGE and the protein was identified by using the ... ambiguous the attribution of the PufXD2)19 strain to the wild-type or the PufDX cluster Isolation of the photosynthetic complexes from the mutant strains As described previously [16], the photosynthetic ... 180 mV (i.e when the ubiquinone pool is fully oxidized) and reflect a dramatic impairment in the redox interaction between the QB site of the RC and the Qo site of the cyt bc1 in the pufX-deleted...

Ngày tải lên: 31/03/2014, 09:20

... subcloned into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5 region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation ... metabolism We have studied the promoter activity of the 5’-untranslated region using fusions with the reporter gene uidA and the presence of DNA-protein interactions in the 5’flanking region of GS1a gene ... step the genomic clone was entirely sequenced and determined to be 2543 bp in length The comparison of nucleotide sequences between the gene and the cDNA [8] showed that the fragment contained the...

Ngày tải lên: 08/08/2014, 14:20

... that the effects of CAP shocks on CUR and RGDP are invariant to the ordering between CUR and RGDP On the other hand, capital flows might affect CUR and RGDP within a quarter, and the CUR and RGDP ... G0(L) (3) Then, the parameters in the structural- form equation and those in the reduced-form equation are related by B(L) = - G0-1 G0 (L) (4) In addition, the structural disturbances and the reduced-form ... consumption and investment in four different periods - the whole period, the 1980s, and the 1990s with and without the Asian crisis period The output volatility is relatively low with a standard deviation...

Ngày tải lên: 24/10/2012, 09:27

... PAI1(T146A) and PAI-1(M149K) Structural analysis Structural analysis was based on the following depositions in the Protein Data Bank ([32]; PDB-ID is given in parenthesis): active (1DVM) and latent (1DVN) ... position at the top of the hF/ s3A-loop right above the inserted s4A while in the stressed conformation of PAI-1stab and a1PI, they stack against the aromatic moiety of the s3A residues Y172 and F189, ... assume that the aliphatic moiety of the introduced K in PAI-1(M149K) allows the side chain to adapt the same orientation as the original M and therefore the stabilizing effect of the M149K substitution...

Ngày tải lên: 20/02/2014, 11:20

... ESCAP is the regional development arm of the United Nations and serves as the main economic and social development centre for the United Nations in Asia and the Pacific Its mandate is to ... cent for the Philippines Countries in the region therefore have lost competitiveness in their exports not only to the United States but also to their other trading partners Since 2006, the countries ... for Indonesia and the Philippines Nevertheless, over the last few years the dependence of the region on the United States market has decreased somewhat Europe now receives about the same share...

Ngày tải lên: 21/02/2014, 01:20

... plant and measure a .the straightened height of the entire plant (from the ground to the top of the tallest plant structure) in meters, b .the number of seed heads within the sample frame, c .the ... samples, and n = number of samples collected The standard deviation indicates the degree of variation in seed weight and is, therefore, a measure of precision (see example) the larger the SD, the ... over the clipped stubble to kill coffeebean and other undesirable vegetation Under either scenario, it is important to inspect the flooded undesirable plants and drain the water soon after they...

Ngày tải lên: 21/02/2014, 12:20

... ASEAN and the Pacific Islands Forum) develop mechanisms to track progress on GBV and SRHR; and • NGOs act as watchdogs and advocate for fulfilling the Cairo agenda and MDG (a & b) targets The last ... Violence and Sexual and Reproductive Health and Rights Gender-based Violence (GBV)1 and young people Young people have the right to be free from violence and to fully attain their sexual and reproductive ... all of these are potentially very limiting to their life choices.11 These can severely curtail educational and employment opportunities and have long-term adverse impact on their own and their...

Ngày tải lên: 05/03/2014, 17:20

... focusing on air, water, and land issues; • Assess environmental releases to air, water, and land resulting from current and projected oil and gas production in the region; and • Lay the groundwork for ... visibility in the Grand Canyon and other park and wilderness areas around the Colorado Plateau 26 All Region states and tribes participate in WRAP One of the organization’s key contributions is the Emissions ... emissions and discharges Nevertheless, the findings in this report demonstrate that on an aggregate basis, the environmental footprint of oil and gas production in Region and other producing regions...

Ngày tải lên: 06/03/2014, 16:20

... that, for the catalysis, only the core HAL ⁄ PAL motif region is required, and the other extended parts serve other (e.g regulatory) functions Therefore the two bacterial PALs in which the extended ... separates the phenolic O-atom of ˚ Tyr110 more than 17 A apart from the exocyclic methylene C-atom of the MIO prosthetic group On the basis of the experimental structures, hypotheses on the role of the ... folding to a Tyr110-loop-out conformation On the other hand, the 20 ps simulations on the Tyr110-loop region of the loop-out structure (i.e a ligand-free experimental 1W27) (Fig 5A,B) indicate...

Ngày tải lên: 07/03/2014, 12:20

... the sum of the 1996 base and the incremental jobs produced over the 1996-2015 period Table 1, Total Direct Economic and Employment Impacts of the LAX Master Plan in the Southern Californian Region ... which is more demand than that handled in any of the other five most populous U.S metropolitan regions In addition to the region s six commercial jet service airports and the two regional (commuter) ... capita in 2015 Other factors also influence the region s demand for air transportation The Southern California region is the largest center for manufacturing in the United States; the headquarters...

Ngày tải lên: 08/03/2014, 04:22

... each other These are 1209 and 1207 obtained from the same otitis media patient on the same day (left and right ear isolates) and 1233 obtained from another patient on a different date The structural ... due to the partial occurrence of PEtn attached to the phosphate group at O-4 of Kdo [11,12,15] Structure of the core region of the Hep3-glycoforms In the 1H NMR spectra of OS-1¢-A (Fig 5A) and OS-1¢ ... between the proton pairs HepIII H-1/HepII H-2, HepII H-1/HepI H-3 (LPS-OH and OS-1¢-B) and HepI H-1/Kdo H-5 and H-7 (LPS-OH) confirmed the sequence of the heptose-containing trisaccharide unit and the...

Ngày tải lên: 08/03/2014, 08:20

... rubber in Asia and the Pacific region FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS REGIONAL OFFICE FOR ASIA AND THE PACIFIC Bangkok, 2011 i The designations employed and the presentation ... introduction of the exotic pests including SALB into Thailand Recently, the PQ Act has been revised to widen the scope of the Act and to strengthen the phytosanitary measures and the 2nd Edition of the PQ ... pose the greatest danger of disease establishment in the region, other pathways need to be examined and their potential risks determined The Plant Protection Agreement for the Asia and Pacific region...

Ngày tải lên: 08/03/2014, 10:20

... European Region They contribute nearly % of the 51 GDP and provide some 2.5 million jobs in the EU [25] These sectors have special importance in the southern countries of the Region, and the low ... economic growth in the 1980s and its overall consequences, along with other factors, contributed to the enormous changes in the CCEE and NIS at the end of the 1980s and the beginning of the 1990s Table ... was the only European Member State among the 20 countries of the world with the highest average annual industrial growth, and the Federal Republic of Germany and the Netherlands were among the...

Ngày tải lên: 15/03/2014, 19:20

... GMD, both the P aeruginosa and A thermoaerophilus RMD sequences have gaps in the region of the RR loop, and in the His6-RMDAt structure, the b2–b3 loop is notably shorter than in GMD The truncation ... enough time for complete conversion of the GDPd-Man, and then removed the enzyme by filtration His6-RMDPa and NADPH were then added to the reaction mixture The GDP-6-deoxy-d-lyxo-hexos-4ulose substrate ... and light sand, respectively; the APPR portion of the cofactor (dark gray) and the ligand analog GDP-DMan (light gray) are represented as space-filling models Termini and secondary structural...

Ngày tải lên: 16/03/2014, 01:20

... input The elasticity should increase as the quality of inputs to the R&D process improves and the spillovers stemming from the R&D activities of other actors in the region become more pronounced The ... their region nearly always also have cooperation partners within the region Hence, there is a positive relationship between the number of cooperative relationships and the spatial extent of the ... Stockholm, and Vienna, the four regions that are dominated by large urban areas, is always grouped in the upper part of the tables to make identification of the special characteristics of these regions...

Ngày tải lên: 16/03/2014, 03:20

... not only the region encompassing the three catalytic residues, but also other regions affect the glucosidic linkage ratio within glucan and oligosaccharide products Results and Discussion The N-termini ... barrel with the three catalytic residues (Fig 1C), determine the types and ratios of glucosidic linkages synthesized (Table 1) The A1 ⁄ O1 and A2 ⁄ O2 regions within the N-termini of the catalytic ... present in the glucan binding domains of GTFA and GTFO [4,5]; black and white bars, predicted locations of the a-helices and b-strands, respectively, corresponding to the relative position of these...

Ngày tải lên: 16/03/2014, 04:20

... positions from the thioglycine (Methanopyrus kandleri) The cysteine following the conserved glutamine (Q) is methylated in the two isoenzymes from Methanothermobacter marburgensis and in the enzymes ... described above, and then subjected to SDS ⁄ PAGE followed by staining with Coomassie Blue The band corresponding to the a-subunit was cut out, distained and dried under vacuum, and the dried material ... Methanothermobacter marburgensis, MCR from Methanosarcina barkeri, and MCR from Methanopyrus kandleri [7] In case of the enzyme from Methanothermobacter marburgensis and Methanosarcina barkeri, the...

Ngày tải lên: 16/03/2014, 05:20

... medicine and are being published by the Regional Office Both the summary and the larger book illustrate the dynamics of the epidemic and its impact on public health throughout the WHO European Region ... of the countries it serves The European Region embraces some 880 million people living in an area stretching from the Arctic Ocean in the north and the Mediterranean Sea in the south and from the ... Ocean in the west to the Pacific Ocean in the east The European programme of WHO supports all countries in the Region in developing and sustaining their own health policies, systems and programmes;...

Ngày tải lên: 16/03/2014, 14:20