1cell signalling networks and potential targets

... determined by ELISA (F) CGP53353 and PD98059 could inhibit PKC bIIand ERK activation, respectively Cells and cell culture Human gastric adenocarcinoma cell line BGC823 and Human umbilical vein endothelial ... angiogenesis depended on PKCbII and ERK1/2 signaling pathway Results STC-1 promotes tumor proliferation and angiogenesis in vivo We successfully constructed BGC/STC cell and BGC/ shSTC cell line SiRNA#2, ... plasmids STC-1 expressions in BGC823 and transfected BGC823 cells (BGC/CON cell, BGC/STC cell, BGC/shCON cell and BGC/shSTC cell) were confirmed in both mRNA and protein (Additional file 1, Figure...

Ngày tải lên: 10/08/2014, 05:21

... hypothesis, the expression of Akt and PrPC was examined by immunohistochemistry and confocal microscope in human gastric cancer cases and cell lines Expression of total and p-Akt was detected in cells ... lg), and exhibited downregulation of P-gp at both the mRNA and protein levels (Fig 4A) Inhibition of the PI3K/Akt pathway decreased the expression of P-gp induced by PrPC at both the mRNA and ... against p-Akt and monoclonal antibody against PrP SGC7901/ADR cells were incubated on glass coverslips for 24 h and fixed with 4% paraformaldehyde in NaCl/Pi The fixed cells were stained and examined...

Ngày tải lên: 07/03/2014, 03:20

... are involved in cytokine and growth factor signalling [13–15] RA is characterized by synoviocyte proliferation and infiltration of inflammatory cells, such as lymphocytes and macrophages, into the ... monocyte/macrophages, and thus their production of IL-1β and matrix metalloproteinase (MMP)-1 [16] A further study showed that A77 1726 inhibits the production of prostaglandin E2 (PGE2), MMP-1 and IL-6 in ... fibroblasts and de-differentiated chondrocytes produce both secreted and intracellular IL-1Ra [28], and in these cells IL-1β-induced IL-1Ra production was enhanced in culture supernatants and in cell...

Ngày tải lên: 09/08/2014, 01:23

... the specialization and experience of the surgical group, not only to lower the morbidity and mortality of the gastrectomy but also because of the complications that can arise and have to be approached ... 77(1):35-41 Seufert RM, Schmidt-Matthiesen A, Beyer A: Total gastrectomy and oesophagojejunostomy–a prospective randomized trial of hand-sutured versus mechanically stapled anastomoses Br J Surg 1990, ... It is a surgery of a high level of complexity that has a risk of death or complication and the dehiscence and fistula of the esophagojejunal anastomosis is one of the most feared complications...

Ngày tải lên: 09/08/2014, 03:22

... M NaOH and heated to 70°C NaOH was added as required to maintain the pH between 7.1 and 7.2, and the solution was filtered and incubated at room temperature with little stirring slowly and continuously ... allopurinol (100 mg), and after the first dose developed acute arthritis of the right foot and ankle Anakinra was administered for days with rapid and complete resolution of signs and symptoms of arthritis ... first injection, and the symptoms and signs of gout were completely abolished 48 hours afterwards Case A 70-year-old man developed arthritis of his right wrist, elbow, hand and shoulder The patient's...

Ngày tải lên: 09/08/2014, 10:20

... animals and humans will be required to determine the full potential of the synergistic effects of both resveratrol and curcumin and their potential for the prevention and treatment of OA and RA ... products (Bcl-2, Bcl-xL and TRAF1) Resveratrol and curcumin inhibited pro-apoptotic proteins (caspase-3, PARP) and matrix degrading gene products (MMP3 and MMP-9), and angiogenesis and inflammation ... collection and interpretation, and the manuscript preparation AM and MS conceived of the study design, and coordinated the studies, data interpretation and manuscript preparation All authors read and...

Ngày tải lên: 09/08/2014, 14:22

... 1-4, weak; and 0, negative In statistical analysis, COX-2 and VEGF-C scores were placed in a high expression group (strong and moderate immunoreactivity) and a low expression group (weak and negative ... Expression of cyclo-oxygenase and 2, prostaglandin E synthase and transforming growth factor beta1, and their relationship with vascular endothelial growth factors A and C, in primary adenocarcinoma ... association between COX-2 and VEGF-C Kyzas et al [51] found that there was a significant correlation between COX-2 expression and VEGF-C expression, and lymph node metastasis in head and neck cancer Timoshenko...

Ngày tải lên: 10/08/2014, 10:21

... analysis, and drafted the manuscript SF, SH, ST, and YY participated in the design of the study and helped draft the manuscript JK, KO, HT, and HF assisted the experiments IN, TF, and TO participated ... lines, and compared with NUGC4, MKN45 and NUGC3 had higher expression of AdipoR1 On the other hand, no significant differences were observed in expression of AdipoR2 (Fig 1B) pg 10 In MKN45 and ... activated monocytes and/ or neutrophils could be responsible for the generation of the globular adiponectin fragment [38] On the other hand, AdipoR1 and AdipoR2 may form both homo- and heteromultimers...

Ngày tải lên: 10/08/2014, 10:21

... by both BHV and LAAO treatment Finally, several gene and protein candidates were elucidated from microarray and SILAC data, such as EIF, HNRNP and HSP families as well as TUBB, TOP2A and SDHA, ... knowledge and skills in toxicology and cancer research, and more importantly encouraged me to be confident and move forward when the project was not going smoothly His attitudes towards work and life ... SDHA, which could be good anticancer targets and deserve further investigations Taken together, this study evaluated and confirmed the anticancer potential of BHV and LAAO from Crotalus adamanteus...

Ngày tải lên: 09/09/2015, 08:13

... Therefore, understanding the pathogenesis and biological features, as well as identification of new markers and therapeutic targets of GC are crucial to improve its detection and therapy Like ... Korea and China), intermediate incidences in Eastern Europe, and parts of Central and South America Incidences are low in Southern Asia, North and East Africa, North America, Australia, and New ... all ages is highest (orange and red) in developing countries such as Asia and South America, and lowest (light and dark green) in North America, parts of Africa, India and Australia Adapted from...

Ngày tải lên: 09/09/2015, 18:49

... East Asia, Eastern Europe, and Central and South America, while relative low rates are found in South Asia, North and East Africa, North America, Australia, and New Zealand (6) Gastric cancer is ... Shang Li and Dr Goh Liang Kee for all the constructive criticism and advice I also thank to Dr Kakoli Das, Mrs Jeanie Wu and Ms Ming Hui Lee for their important technical support, advice and kind ... differentially expressed x between the sensitive and resistant gastric cancer cell lines group Two gene candidates, STAT1 and RNH1, were nominated and subsequently validated at the protein level...

Ngày tải lên: 10/09/2015, 09:06

... every 21 days) and cisplatin (75 mg/m2 every 21 days), response rates were 35% and stable disease was 17% The therapy was well tolerated and no grade toxicities were seen The first randomized, controlled ... progression-free survival (PFS) and a small survival advantage over cisplatin and 5FU (CF) (9.2 vs 8.6 months; P.02) (8) However, increased toxicity was seen (such as neutropenic infection and diarrhea), especially ... internationally accepted standard of care; monotherapy with 5FU or doublets with 5FU and cisplatin, irinotecan or an anthracycline, are reasonable alternatives for patients who are not candidates for ECF...

Ngày tải lên: 06/03/2014, 02:21

... used for standardizing PTEN and Egr-1 mRNA Amplification primers were 5¢-ACCAGTGGCACTGTTGTTTCAC-3¢ and 5¢-TTCCTCTGGTCCTGGTATGAAG-3¢ for the PTEN gene [43], and 5¢-AGCCCTACGAGCACCTG-3¢ and 5¢-CGGTGGGTTGGTCATG-3¢ ... 15–22 days, individual colonies were isolated and expanded, and the expression of PTEN was examined by western blotting (C) Colony was treated with VD3, and apoptosis was evaluated (D) *P < 0.05, ... VD3 and 200 nM TSA (A), or VD3 and mM NaBu (B), and western blotting was used to evaluate PTEN expression Cell lines stably transfected with PTEN siRNA or control siRNA were treated with VD3 and...

Ngày tải lên: 22/03/2014, 21:20

... Surgery and radiotherapy Surgery and chemotherapy Surgery and radiotherapy and chemotherapy Hormonal therapy dated for oncology clinical research [29-31] It has also been validated [32] and found ... subgroup The EQ VAS on the other hand was able to discriminate between the 'no change' subgroup and the subgroups reporting a moderate and large improvement and moderate and large deterioration Discussion ... contributions MK was responsible for the data collection and drafted the manuscript MK works under direct supervision of CD and LB PL, CD and LB read and corrected draft versions of the manuscript 16...

Ngày tải lên: 18/06/2014, 19:20

... pelvic radiotherapy and placebo, rates of Grades 1, 2, and diarrhea during treatment were 55, 42, and 3%, respectively For the 32 eligible patients who received radiotherapy and lactobacillus acidophilus ... formed, soft, and liquid stool was 0%, 35% and 65%, respectively in placebo group In contrast, in the study drug group the prevalence of formed, soft, and liquid stool was 3%, 78% and 19%, respectively ... Thailand, 2Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand and 3Department of Statistics, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand...

Ngày tải lên: 09/08/2014, 08:23

... IL-6, and IL-23), chemokines (CCL3, CCL5, CCL7, CXCL1, CXCL2, and CXCL5), and growth factors (BMP-2, BMP-6, BMP-7, and FGF-9) as well as proteins involved in the synthesis of prostaglandin E2 and ... First-strand synthesis and second-strand synthesis were performed following the manufacturer's instructions The second-strand product was cleaned (GeneChip Sample Cleanup Module; Affymetrix) and used ... conceived of and carried out the study EG and SCG participated in the study design and coordination MPP performed the array hybridization and the data analysis MLB, EAD, JDK, PPL, KRP, JSS and RSW...

Ngày tải lên: 09/08/2014, 08:23

... maximum diameter less than or equal to cm, and stage III was defined as numerous and large metastases or the presence of hepatomegaly and/ or ascites (Table and Figure 1) Among 89 patients reviewed, ... standard care, and many physicians decide against postoperative CCRT even at high risk of local recurrence Despite the consensus that adjuvant pelvic RT provides survival benefit for stage II and ... one, followed by a bolus of 5-FU at 400 mg/m2 and LV at 200 mg/m2 on days one and two with infusional 5-FU at 600 mg/m2 for twentytwo hours on days one and two The FOLFIRI regimen consisted of irinotecan...

Ngày tải lên: 09/08/2014, 09:20

... combination (Table 2, a) and then divided according to treatment regimen and analyzed separately (Table 2b and 2c) A total of 154 and 94 patients received treatment A (4.3 Gy *10) and B (2.5 Gy *20) ... both materials, and the top three accounted for 73.6%, 79.9% and 71.3% of the total number of represented chromosomes when analyzing cases and controls combined, only controls and only cases respectively ... VNK and ALBD designed the study - TT, LJ and PV genotyped the samples from the American Breast cancer patients - LJ and PV genotyped the samples from the Norwegian breast cancer patients and...

Ngày tải lên: 09/08/2014, 10:21

... Research and Therapy 2010, 7:42 http://www.aidsrestherapy.com/content/7/1/42 Page of cellular reservoirs for HIV-1 under therapy [20] Baldanti and colleagues show that naïve and memory cell numbers and ... assay [9] This assay targets the LTR segment of the virus genome where the second strand transfer takes place and quantifies only fully reverse transcribed HIV-1 genomic DNA and has high specificity ... (A, C, D and F) and demonstrated a wide range of viral load values and CD4 counts (Table 1) Three out of five study subjects received an RT inhibitor (AZT), one a protease inhibitor (APV) and one...

Ngày tải lên: 10/08/2014, 05:21