17 ido and tryptophanyl trna synthetase tts mrna levels in antigen sensitised cells by qrt pcr
Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
Ngày tải lên :
09/08/2014, 10:23
... broad-range PCR and/ or reverse transcription PCR systems to search for bacterial DNA and RNA in synovial samples from patients with various forms of arthritis, including ReA [12,14 ,17] By cloning and ... contamination during DNA extraction and manipulation These included pipeting PCR components under a laminar flow of sterile air, using only sterile equipments, dedicated pre -PCR and post -PCR rooms, ... at 94°C for minute, primer annealing at 59°C for minute and extension at 72°C for 1.5 minutes The final elongation step was extended to 15 minutes PCR was carried out in a Gene-Amp PCR System 9700...
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Báo cáo khoa học: Spermosin, a trypsin-like protease from ascidian sperm cDNA cloning, protein structures and functional analysis doc
Ngày tải lên :
17/03/2014, 11:20
... chain but are distinct in the length of light chains: one contains a long L1 chain and the other contains a shorter L2 chain Furthermore, the prolinerich region in the L1 chain is capable of binding ... the prolinerich regions located in the C terminals of human and porcine proacrosins play a key role in interaction between proacrosin and the zona pellucida [18] To investigate the binding ability ... for protein structures of preprospermosin and spermosin type and type are depicted in Fig 3C Binding of spermosin light chains to the vitelline coat Comparison of the sequences between L1 and L2...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
Ngày tải lên :
07/08/2014, 18:21
... 14 17 17 22 95.4 100 95.2 93.9 91.4 91.4 91.1 15 15 15 14 16 15 16 17 16 1 28 24 95.4 95.7 92.9 91.8 91.6 90.3 15 15 15 13 13 16 14 15 16 17 17 22 17 95.2 93.9 91.4 91.4 91.1 17 17 17 16 17 15 ... from provinces of Korea were identified as PCR positive Most of the ticks (896/1,288) investigated in this study originated from the rice fields and army training sites of Gyeonggi province Other ... the predominant species of tick and often found in association with humans and animals in Korea [18] The prevalence of E chaffeensis infection in 4.3% ticks observed in this study indicate that...
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Báo cáo y học: " Comparative evaluation of INNO-LiPA HBV assay, direct DNA sequencing and subtractive PCR-RFLP for genotyping of clinical HBV isolates" pot
Ngày tải lên :
12/08/2014, 04:20
... shared the amino acid pattern Arg122, Pro127 and Ser140 Page of specifying ayw subtype with unique amino acid substitution involving residue 134 (Ile134 or Asn134) The remaining strain contained unique ... strains Additionally, the PCR- RFLP-based method also detected the Genotype A in all 13 mixed Genotype A and D strains of HBV On the other hand, despite being costly or technically demanding, ... therefore, cloned in pGEM-T Easy plasmid and 10 independent recombinant plasmids for each HBV strain were sequenced The DNA sequencing data showed that all the seven strains contained a T to C nucleotide...
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Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... (A) Initial PCR amplification product using two original flanking primers (primers and 2); (B) nested PCR from the primary PCR amplification product using one original flanking primer (primer 1) and ... proteins encoded by ORFs (open reading frames) generated by genome-sequencing projects In this era of genome sequencing there is an increasing demand to assign functions to ORFs and the in vitro ... strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99 Template DNA PCR with one biotinylated primer B Biotinylated PCR product Bind...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
Ngày tải lên :
20/02/2014, 02:21
... Using Parsimony ( *and Other Methods), 4th edn Sinauer Associates, Sunderland, Massachussetts 24 Laing, K.J., Holland, J., Bonilla, S., Cunningham, C & Secombes, C.J (2001) Cloning and sequencing ... recorded in both head kidney and liver This indicates that the gene is inducible by LPS stimulation Administration of endotoxin induced IL-10 production in mice, chimpanzees, baboons and humans ... effects of endotoxin challenge and reduces the levels of TNF, IFN-c and macrophage in ammatory protein-2 [36] Although the expression study indicates that this cytokine is involved in the immune...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
Ngày tải lên :
07/03/2014, 21:20
... other known cysteine-containing chitin-binding domains [14], such as plant chitin-binding proteins and horseshoe crab antimicrobial peptides with chitin-binding affinity [15 17] Rebers and Willis reported ... chitin-binding proteins in T tridentatus Amino acid compositions of high molecular mass (HMM) chitin-binding proteins HMM chitin-binding proteins resolved by 2D SDS ⁄ PAGE were categorized into ... with asterisks Numbers on the right indicate amino acid residue numbers and DE29 is involved in chitin binding In insects, cuticular proteins containing cysteine residues have not been reported,...
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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
Ngày tải lên :
08/03/2014, 08:20
... 2003 Fig Protein binding specificity and protein contact sites of the D-Term DNA (A) DNAse footprinting using mt protein extract Binding reactions contained 3¢ end-labelled H-strand D-TERM DNA ... loading buffer, and sequentially with a step gradient (4 mL each) containing 0.2, 0.5 and 1.0 M KCl in loading buffer Protein eluted in each step was tested for binding to the putative termination ... bidirectional [18] In keeping with their different binding specificities, the two terminator sequences seem to bind to distinctly different proteins The D-TERM DNA binding proteins purified by affinity...
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DNA Sequencing – Methods and Applications Edited by Anjana Munshi pptx
Ngày tải lên :
08/03/2014, 19:20
... methods for determining the order of the nucleotides bases adenine,guanine,cytosine and thymine in a molecule of DNA The first DNA sequence were obtained by academic researchers,using laboratories ... sequencing utilizes labelling of the chain terminator ddNTPs,which permits sequencing in a single reaction,rather than four reactions as in the labelled- primer method In dye- terminator sequencing ... now the mainstay in automated sequencing Its limitation include dye effects due to differences in the incorporation of the dye-labelled chain terminators into the DNA fragment,resulting in unequal...
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Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
Ngày tải lên :
23/03/2014, 13:20
... Analysis of DNA binding by hydroxyl radical footprinting and interference assays A more detailed picture of the binding of HAKN1 to its target site was obtained by the analysis of footprinting patterns ... both binding sites in the complexes formed by KNOX and Bell proteins Sequences outside the homeodomain may also in uence the binding properties of the protein Indeed, a stretch of 16 amino acids ... TGACA and variants of this sequence It is logical to assume that changes in amino acids involved in the interaction must in uence binding efficiency In addition, some substitutions may alter binding...
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Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx
Ngày tải lên :
30/03/2014, 04:20
... No.30470379) in China References Albagli O, Dhordain P, Deweindt C, Lecocq G & Leprince D (1995) The BTB ⁄ POZ domain: a new protein–protein interaction motif common to DNA- and actin-binding proteins ... human acute T-cell leukemia cells were maintained in RPMI-1640 medium containing 10% fetal bovine serum (Invitrogen) All these cells were incubated in a humidified 5% CO2 incubator at 37 °C Creation ... constructs in A549 and DU145 cells M-U indicates that the NEG-U element was mutated in F-560, M-D indicates that the NEG-D element was mutated in F-560 and M-B indicates that both the NEG-U and NEG-D...
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Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc
Ngày tải lên :
31/03/2014, 01:20
... of arginine side chains in proteins Protein Eng 6, 247–259 23 Jones, S., Marin, A & Thornton, J.M (2000) Protein domain interfaces: characterization and comparison with oligomeric protein interfaces ... measured kinetic stabilities Arginine residues are known for their prevalence in both intra- and inter-chain interfaces [22,23] where the charged guanidinium group is often involved in formation ... analysis of phosphate and sulphate binding sites in proteins Estimation of propensities for binding and conservation of phosphate binding sites J Mol Biol 242, 321–329 22 Nandi, C.L., Singh, J & Thornton,...
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bioinformatics sequence and genome analysis - david w. mount
Ngày tải lên :
08/04/2014, 12:44
... isoleucine lysine leucine methionine asparagine proline glutamine arginine serine threonine valine tryptophan undetermined amino acid tyrosine either glutamic acid or glutamine Adapted from IUPAC-IUB ... comprising interacting  strands, and loops (Fig 1.7) This folding often leaves amino acids with hydrophobic side chains facing into the interior of the folded molecule and polar amino acids that can interact ... or T Guanine Adenine Thymine Cytosine puRine pYrimidine aMino Keto Strong interactions h bonds Weak interactions h bonds H follows G in alphabet B follows A in alphabet V follows U in alphabet...
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Báo cáo hóa học: " Research Article Binocular Image Sequence Analysis: Integration of Stereo Disparity and Optic Flow for Improved Obstacle Detection and Tracking" pptx
Ngày tải lên :
21/06/2014, 22:20
... some colour discontinuity occurs in the same object indicating mismatching points By introducing optic flows as extra constraints, we are defining multiple measures for stereo matching As well as the ... measure Applying the epipolar line constraint, we can search the correspondence point in the same row For a seed point at position (il , j) in the left edge image and a candidate point at position ... false matching points eliminated 3.1 FUSION USING A KALMAN FILTER FOR 3D OBJECT TRACKING Modelling For a point P(X Y Z) in the world coordinate, the state vector to be tracked is defined by SV =...
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Báo cáo hóa học: " Autoregressive Modeling and Feature Analysis of DNA Sequences" pot
Ngày tải lên :
23/06/2014, 01:20
... coding and noncoding regions for AR model orders 15 and 35, using binary SW mapping The distribution densities obtained by using real-number mapping are depicted in Figure 11 Both coding and ... of gene 17 is used for linear prediction of 50 noncoding segments using binary mapping (a) Error in noncoding sequences 1–12; (b) error in noncoding sequences 13–25; (c) error in noncoding sequences ... used for linear prediction on 50 noncoding segments using binary mapping (a) Error in noncoding segments 1–12; (b) error in noncoding segments 13–25; (c) error in noncoding segments 26–38; and (d)...
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Báo cáo khoa học: " Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor" doc
Ngày tải lên :
07/08/2014, 15:20
... canine LINE-1 and the TVT-LINE The ORF2 of caine LINE-1 has one open reading frame from 178 to 4002 nucleotide The DNA sequences of the TVT-LINE in TVT lines are compared with those of canine LINE-1 ... to the complementary strand of canine LINE-1 and was followed by a 5bp deletion; DNA sequences of the TVT-LINE showed a 98 % and 63 % homology to canine LINE-1 and human LINE-1 sequences, respectively ... Can in e LINE-1 ORF2 To obtain the functional canine LINE-1 sequence, RT -PCR was performed with mRNA from cell line A72 The ORF2 was constructed from six overlapping PCR fragments The six PCR...
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Minireview Maize DNA-sequencing strategies and genome organization Ron J Okagaki and Ronald L ppt
Ngày tải lên :
09/08/2014, 20:20
... positional information, and markers for high-resolution mapping These are the obvious reasons for wholegenome sequencing, but others may in fact prove more rewarding We now know that different maize lines ... of genes In one region sequenced from two lines, four of the ten genes present in one line were absent from the other [17] Tandem duplications provide an opportunity for gene number to increase ... genome sequencing project Plant Physiol 2001, 127:1572-1578 Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, et al.: Initial sequencing and analysis...
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Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx
Ngày tải lên :
09/08/2014, 20:20
... recovered by Baermannization in saline at 37°C, washed × in saline and × in RPMI1640 containing 100 µg/ml penicillin and 100 U/ml streptomycin Worms were incubated with 10% gentamicin for 20 and then ... to inhibit host antigen- processing pathways [29] Structural genes for both tubulin [30] and a keratin-like protein [31] have been described, and an αcrystallin-like small heat-shock protein (Hsp20) ... Secreted and intracellular globins have been characterized [25], and genes for both secretory [26,27] and neuronal [28] acetylcholinesterases cloned A recombinant cystatin (cysteine protease inhibitor)...
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