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... Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14, ... and msx-3d [12]) with a mass tolerance of p.p.m We therefore used MALDITOF-TOF and ⁄ or nanoLC-Orbitrap tandem MS to further identify peptides from this list Twenty-five mono-linked peptides and ... peptides The list of light and heavy precursor masses was further used either to analyze the MS ⁄ MS spectra acquired in the data-dependent acquisition analysis or to build an inclusion list with the...

Ngày tải lên: 15/03/2014, 00:20

... Rab4 and Rab5 The interaction of Rabaptin-5 with the small GTPases Rab4 and Rab5 can be assessed readily in the yeast two-hybrid system [12,14] We therefore used this assay to analyze the interaction ... Rabaptin-5d can interact only with GTP-bound Rab5 and not with GTP-bound Rab4 To address the question of how relevant these findings are to the protein interaction properties in vivo, we next examined the ... brief, the yeast strain Y153 was used to cotransform bait and prey plasmids, and transformants were selected by plating onto synthetic dextrose (SD) leucine and tryptophan dropout plate To evaluate...

Ngày tải lên: 16/03/2014, 18:20

... contrasts with the change in environment of the label upon interaction of actin with the hcM398–414 myosin loop peptide The markedly different response of the IAEDANS label to the binding of the two ... is the inhibitory region of TnI which on binding to actin renders the molecule unable to bind to the N-terminus of MLC1 and the myosin loop peptide and possibly other sites of interaction with ... the head of the myosin molecule, can bind to the C-terminal of actin [15,37,38] The MLC1 peptides were found to bind to F-actin both in the absence and presence of tropomyosin with the interaction...

Ngày tải lên: 17/03/2014, 10:20

... attachment to C jejuni strain L4 (Fig 2a, b) Bacteriophage PV22 interacted with C jejuni by attachment followed by translocation of the phage to the polar region of the bacterium up to the point ... capillary method with chicken epithelial cecal cells as the attractant with phage PV22 at an MOI of 10 with C jejuni or C jejuni alone Competing interests The author(s) declare that they have no ... onto colloidal supporting films and treated with 1% uranyl acetate for further examination by electron microscopy (Hitachi H300) utilizing standard methods [32] The number of phage that bound to...

Ngày tải lên: 20/06/2014, 01:20

... media, or other natural phenomena Early radars were “blob” detectors in that they detected the presence of a target and gave its location in range and angle, and radar began to be more than a blob ... )|, then f ym = ym ym exp − 2 γ2 + σ +σ E E2 γ (35) The mean value of ym is π(E2 γ2 + σ )/2 and the variance is (4 − π)(E2 γ2 + σ )/2 The variance of signal is (4 − π)E2 γ2 /2 and the variance ... detector and could provide recognition of one type of target from another [7] It is known that small changes in the aspect angle of complex (multiple scatter) targets can cause major changes in the...

Ngày tải lên: 22/06/2014, 22:20

... brand management strategy The Director is the one who decides on all branding activities of the Company with the assistance of his young brother, the Company’s Vice Director When asking about the ... Company has to build its own brand name and manage it well This study will see how the Company manages its brand as an effective weapon to compete with other competitors 1.3 Objectives of the ... on analyzing brand management of the Company Its specific objectives are: • To analyze the external market environment of the auto voltage stabilizer industry and the Company situation • To analyze...

Ngày tải lên: 13/04/2013, 10:29

... contrast to many French theorists, Frank sought to redeem a humanist position, to save the individual as an autonomous and moral agent, and to oppose the potential totalitarianism of a ubiquitous and ... identify with Beckmann’s inability to adjust to peacetime And the producer rejects Beckmann’s strange and frightening performance, advising him to practise further, and that truth has nothing to with ... experience, the history, the reality The rhythms and figures that Celan has woven into his ‘Todesfuge’ may deflect from the very theme of the poem, distancing us, or bemusing us, enchanting us, rather than...

Ngày tải lên: 01/11/2013, 09:20

... fisheries and used the recent PROCFish surveys to help the participants to understand stocks in their countries in order to adapt their management plans ORGANIZATION OF THE WORKSHOP Preparation The ... prospectus for the workshop The agenda and prospectus were sent to the participants prior to the workshop (Annexes A and B) The workshop centred on the principles within the ACIAR and FAO manuals Each ... fisheries The agenda and facilitators led the participants through the ACIAR sea cucumber manager’s toolbox (Friedman et al., 2008) to review indicators of stock status and apply them to their fishery...

Ngày tải lên: 14/03/2014, 10:12

... The Art To Influencing Difficult People How To Handle These Difficult People The Intimidator The Stealth Bomber The Oscar Goes To Person The Bombastic Blowhard The Politician The Yes People The ... so they can see them, in a meaningful manner, without seeming as if you are patronizing them By showing the person you see the good in them, and respect them, they will be easier to deal with, ... to the topic they introduced is another way to show that you are interested in the conversation, and in what the speaker has to say about that topic Get your point across by using the words the...

Ngày tải lên: 20/03/2014, 19:20

... channel to be able to guarantee that the EMS can read them safely One alternative is simply to abandon the vote tallying aspect of the EMS entirely and manually add the vote totals reported by the ... accuracy can be achieved without assistance, so with existing devices it must be OCRed and then manually checked and corrected — there is not enough redundancy in the tapes to allow automatic error ... electronically and on the VVPAT record; if the voter notices, then the DRE could allow them to change their vote and could behave honestly from then on for that voter In this way, the electronic and VVPAT...

Ngày tải lên: 23/03/2014, 13:20

Ngày tải lên: 20/08/2015, 00:38

... with at least of the following symptoms: relieved with defecation associated with change in frequency of stools Associated with change in form of stools and two or more of the following symptoms: ... several mechanisms operating between the gut and the brain, and conceivably, an alteration of these mechanisms could result in bloating sensation The tolerance of mechanical stimuli in the gut depends ... at least two of the following symptoms: relieved with defecation associated with change in frequency of stools Associated with change in form of stools with the following symptoms supporting...

Ngày tải lên: 26/09/2015, 10:05

... 1.6) Topology and Geology Topologically and geologically, the mainland of the study area can be separated into two regions: the lowlands in the Western part and highlands in the Eastern part The ... the order of importance of the factors/processes provided by the model and to compare them with the experts’ experiences Therefore, the total BOD load to the coastal sea and the living coral area ... represents the degree of confidence that the analyst associates with the answers of the expert i, to a certain set of questions related to an objective variable; xi is the ranking of a factor/process with...

Ngày tải lên: 06/11/2012, 10:35

... guaranteed to have found this edge, and can use it to construct the copied conjunct over spans to and therefore the whole co-ordinated NP from spans to To simplify the implementation of the copying ... general mechanism, and that the copy mechanism is not needed This conclusion is strengthened when we turn to consider the performance of the parser on the standard Penn Treebank test set: the Within ... explains the difference in performance between the Between and Within models and the Copy model when beam search was used We therefore also state the performance for Between and Within models with...

Ngày tải lên: 20/02/2014, 12:20

Ngày tải lên: 05/03/2014, 16:20

... that in the commodity transportation sector the value of one hour is equal to 0.0000848 times the value of the goods transported The VOT is thus found to be proportional to the value of the goods ... establishing the effects of the concentrations measured in the air and the extent to which the pollution caused by the additional vehicle-km aggravates these effects In the literature the effects of the ... consists of analyzing the consequences of the change in emissioils due to the additional car-km If the emissions of the transport sector arc larger than in the initial situation, emissions have to decrease...

Ngày tải lên: 07/03/2014, 11:20

... before, the current and the iterations after the current iteration) to smooth the scores We call this median9 We choose the iteration with the best smoothed score as the stopping point for the filtering ... 180 transliteration pairs and 2084 non-transliteration pairs and the English/Arabic gold standard contains 288 transliteration pairs and 6639 non-transliteration pairs We have submitted these ... of the training data as g2p does The training data contains more non-transliteration pairs than transliteration pairs We don’t want to maximize the likelihood of the non-transliteration pairs...

Ngày tải lên: 23/03/2014, 16:20

... to steal work from the victim’s agenda A thief will steal half of the work on the agenda This balances the load between the two threads and minimizes the chance that either thread will have to ... Critical path analysis for type and type task graphs (average and worst case) the path The critical path length T∞ can be defined as the longest path between any two nodes in the graph The presented ... case, and T∞ is the critical path Furthermore, to effectively ¯ use P processors, the average parallelism P = T1 /T∞ should be larger than P The first step of the analysis is to find an appropriate...

Ngày tải lên: 31/03/2014, 04:20

... overall performance of the sectors themselves Then the two strategies are compared to each other and performance of the two groups of managers with respect to their own strategies can be compared Exhaustive ... produces the principal policies and plans for achieving those goals, and defines the range of business the company is to pursue, the kind of economic and human organization it is or intends to be, and ... military manpower, with the commitment of 10 million men to the European theater and only million to the Pacific until the defeat of Germany permitted a reallocation Today, however, the term “strategy”...

Ngày tải lên: 03/06/2014, 01:55

... in I, and where μL(E) = (1/N) N L Ei ) denotes the average luminance of i= the mean-offset distortions E The quantities L Ii ) and L Ei ) correspond to the luminance of the ith pixel of the image ... computed with respect to the mean luminance of the background-image patch (mask) Let I and I denote an original and distorted image patch, respectively, and, let E = I − I + (1/N) N Ii denote the mean-offset ... curves We too have left g as a free parameter 3.1.2 The Excitatory and Inhibitory Exponents p and q, and the Semisaturation Constant b The parameters p and q, when used with (1), are designed to account...

Ngày tải lên: 22/06/2014, 00:20