... disputable relevance of our
results to pathogenesis and clinical manifestations of
atherosclerosis. The finding of C. pneumoniae in serum
of ACS patients does not establish causality for the
pathogen ...
from the same volume of whole serum with QIAmp
Blood Midi Kit (QIAGEN, Valencia, CA) according to
the manual.
Bacterial DNA was also extracted fromthe bac-
terial particles trapped fromthe ... may originate from destructed
mononuclear cells and/or macrophages residing in
the atherosclerotic plaques. However regardless ofthe
origin, we can claim that the presence of virulent
chlamydial...
... final part of the
outbreak data, as there is often a period lasting ten or more weeks when there are
few cases.
We investigated whether the removal ofthe lowest-valued points fromthe data
sets ... differences between the behavior ofthe SIR model and the data. Potential
sources of these differences include inadequacies ofthe mathematical model to de-
scribe the process underlying the data and ... proportional to the square ofthe predicted incidence: w
j
= 1/[z(t
j
; θ)]
2
.
On the other hand, if ρ = 1/2, then the weights are proportional to the rec iprocal
of the predicted incidence; these correspond...
... is the cross-sectional average ofthe bank-specific variable α
i
of those banks for
which there is an observation in month t. As the composition of this sample changes in the
course of time, the ... (column 3) and
the model with only the regulation variables (column 4). The R
2
of the full model is
17.24%, i.e. the combined contribution ofthe systematic factor and the regulation to the
total ... constant
and the own funds decrease or increase.
With the help ofthe two other specifications, it is possible to disentangle the contri-
butions ofthe systematic factor and ofthe regulation....
... applying the optical principle
of the persistence ofthe image on the retina, as also the continuous progress of the
syntheses of movement as compared with the state of photographic techniques. The
fact ... to have the last word in the ar-
gument with death by means ofthe form that endures. If the history ofthe plastic
arts is less a matter of their aesthetic than of their psychology then it will ... of all is the obstinate
resistance of matter to ideas rather than of any help offered by techniques to the
imagination ofthe researchers.
Furthermore, the cinema owes virtually nothing to the...
... catalytic residues
The active site cavity in IPDC extends fromthe thiazolium
ring ofthe cofactor to the surface ofthe protein. The
entrance ofthe active site cleft is covered by the C-terminal
helix ... on the other hand involving
the C-terminal part ofthe polypeptide chain (Fig. 7). In the
latter, differences in the conformation ofthe loop between
Fig. 6. Stereo picture ofthe model of the
a-carbanion/enamine ... However, the packing
of the two dimers in the tetramer is different from that of the
PDCs of known structure, best described as a 20° rotation
of one dimer towards the other when compared to the
Z....
... NeuAc.
Discussion
The presence of naturally occurring agglutinins in the
hemolymph of several crustaceans has been well known
since the beginning ofthe 20th century [34]. An evaluation
of the literature ... °C. The eluant was
collected at a rate of 0.6 mLÆmin
)1
.Thecolumnwas
washed with HSB until the A
280
of the effluent was < 0.002.
The column was further washed with LSB at 4 °C until the
A
280
of ... containing 100 lLof100m
M
CaCl
2
at a rate of
0.3 mLÆmin
)1
. The presence of calcium chloride was
required in the collected fractions because the lectin was
unstable in the presence of EDTA. The fractions...
... proteins
implicated in the biosynthesis ofthe aminonucleoside moiety
of A201A. Here we report the sequencing of a total of
6946 bp, which include the ata genes most likely implicated
in the biosynthesis of this ... it. Further sequencing ofthe ata
cluster could provide an insight into the biosynthetic
pathway ofthe other moieties of A201A and provide
probes which may be useful to identify genes of the
hygromycin ... in the biosynthesis
of the
D
-rhamnose and a-p-coumaric acid moieties of
A201A. Further sequencing of 2 kb of DNA downstream of
ard1 has disclosed a region which might contain one end
of the...
... 269) 427
[26,27]. Then, the biosynthesis ofthe core region of LPS
from A. baumannii strain ATCC 19606 should proceed with
the introduction of a third Kdo residue to O-5 ofthe second
Kdo. This ... mechanism of
the transfer ofthe third Kdo residue has not yet been
identi®ed.
In addition to the work on the determination of the
chemical and antigenic structures of O-speci®c polysaccha-
rides from ... to a complete
structure ofthe carbohydrate backbone ofthe LPS from
A. baumannii strain ATCC 19606 as depicted in Fig. 4.
The structures o f four core regions from LPS of d ifferent
Acinetobacter...
... locks the enzyme in the active form for CTP synthesis and
thereby stimulates the k
cat
of the reaction. Since GTP actually will
activate the uncoupled glutaminase reaction the structure of the
monomer ... was
similar to that ofthe overall CTP synthesis reaction. It
was concluded that the effect of GTP was mainly to
enhance the rate of chemical steps ofthe glutaminase
reaction. The finding that the k
cat
value ... as the displacement ofthe baseline. The peaks
observed at each injection time are derived fromthe heat of dilution
of glutamine into the reaction cell. (B) The heat generated by hydrolysis
of...
... over the minds ofthe children by means of
these dolls. She fell at once into the idea ofthe children in regard to them, and treated them always as if they
were real persons; often speaking of them ... perseveringly followed up by the watchful efficiency ofthe mother. We can not substitute
the action ofthe child for that ofthe parent in the work of early training, but we can often derive very great
advantage ... a wood. Either of them led to the river. But there was a brook to cross, and
for one of these paths there was a bridge. There was none for the other. George said that they would take the
former....
... P
i
. The effect of these samples on cyto-
kine production by PBMCs activated by LPS was then
assessed as described above.
Prediction of interaction sites
The prediction of binding sites fromthe ... amphipathicity) ofthe helices. The
mean surface accessibility ofthe predicted epitopes on the
Iris 3D structures was calculated by averaging the accessible
surface area ofthe peptide residues using the Shrake ... meal from a variety of verte-
brates, ranging from amphibians to mammals, and
including domestic animals and humans [2]. It is the
one ofthe vectors of Borrelia burgdorferi, the agent of
Lyme...
... molecular mass ofthe native peptide was
consistent with the oxidation ofthe corresponding thiol
groups, in order t o form the last missing disulfi de bridge.
Furthermore, this is one ofthe c onstant ... shown thereafter. The first group
(11 sequences) is fromthe a-ScTXs, t he second is fromthe b-ScTXs. Birtoxin is the sho rtest. The de pressant and the long-chain ex citatory are in the
last two ... ofthe N-terminal segment with C5 ofthe b2 stran d [ 9].
The fourth disulfide bond is established between C1 and C8,
of the N- with t he C-terminal region. The excitatory insect
toxins lack the...
... lower than
that ofthe L -rich apoferritin of horse spleen (t
1/2
¼ 60 0 s)
[19]. The iron-oxidation capacity is maintained by
T. bernacchii apoferritin at low temperature; the rate of
the reaction ... Interest-
ingly, the 262 nm phenylalanine band is of opposite sign t o
the protein a t pH 7.0 (Fig. 4B). The f ar-UV CD spectrum
of T. bernacchii apofer ritin at pH 2.5 shows a modest blue
shift ofthe zero ... fish
ferritins on the basis ofthe available sequences.
A further distinctive property of t he fish H chains known
to date relative to those o f mammals appears to be the lack
of the four-amino-acid...
... electronic condition of
the Fe-S cluster through the protein scaffold, resulting
in an alteration ofthe signal rhombicity ofthe EPR
spectrum. The divergence ofthe g-value from that of
the whole cell ... upstream
of the GAF domain are the potential ligands of the
Fe-S cluster. Consequently, the binding ofthe ATP
analog and the subsequent rearrangement ofthe inter-
domain interaction affects the ... level ofthe lacZ gene immediately after the
addition of o-phen to minimize the effect ofthe growth
inhibition by o-phen on the transcriptional activity of
VnfA. Figure 5 shows the time course of...
... APTT
reagent in the absence of Ca
2+
. In these experiments,
the kallikrein–kinin system had been activated before
the addition of triafestin-1 or triafestin-2. In the
absence of Ca
2+
, factor ... chro-
mogenic substrates. Neither triafestin-1 nor triafestin-2
inhibited the amidolytic activities of any of these
plasma proteins (data not shown). Therefore, we exam-
ined the effects of triafestin-1 and ... HK. The fact that
these three unrelated blood-sucking arthropods share
such similar bioactive substances as major components
of the saliva emphasizes the biological importance of
inhibition of the...