10 tumors showing both germ cell and gonadal stromal elements

... human epithelial cell lines Cells from a human bronchial epithelial cell line (Calu-3) and from a human alveolar epithelial cell line (A549) were used for the present studies Both cell lines were ... lymphocytes and 5–20% monocytes The cells were resuspended in cell media with 1% FCS to million white blood cells/ml and plated in 48 well cell culture polystyrene clusters (Corning) and cultured ... Calu-3 cells Conditioned media and transwell studies PBMCs and lung epithelial cells were cultured alone in cell media with 1% FCS for 18 hours The cells were centrifuged at 200 g for minutes and...

Ngày tải lên: 12/08/2014, 15:21

... 4.0% for renal cell carcinoma, 0.84% to 3.6% for transitional cell carcinoma, 0.4% for germ cell tumors, and 0.36% to 0.7% for prostate adenocarcinoma[9] Testicular mixed germ cell tumors are ... testicular mixed germ- cell tumors is because germ cells in the testes are totipotent and can undergo either trophoblast or somatic differentiation Among all the subtypes of testicular germ cell tumor, ... case reports and review of literature J Cutan Pathol 2009, 37(4):486-90 16 International Germ Cell Consensus Classification: A prognostic factor based staging system for metastatic germ cell cancers...

Ngày tải lên: 09/08/2014, 03:21

... programmed necrosis and virus-induced inflammation Cell 2009, 137:1112-1123 Declercq W, Vanden Berghe T, Vandenabeele P: RIP kinases at the crossroads of cell death and survival Cell 2009, 138:229-232 ... [31]) When analyzed microscopically, both untreated cell lines uniformly displayed an intact cell morphology with round cells and almost no irregular shapes or cell debris (Fig 1B, left panels) ... W, Vandenabeele P: Death receptor-induced apoptotic and necrotic cell death: differential role of caspases and mitochondria Cell Death Differ 2001, 8:829-840 Annis MG, Yethon JA, Leber B, Andrews...

Ngày tải lên: 09/08/2014, 10:20

... patient to make a fist and then to relax the hand completely Slowly go through muscle groups, starting with the feet and working through the entire body, first tensing and then relaxing b) Help ... how it feel, to have muscle tensed and muscle relaxed Distraction for both procedures and anxiety a) Invite patients to use whatever distractions are available and work for them (e.g., television, ... Helpful for All Patients A Relaxation and distraction are powerful psychological interventions and have been found to reduce pain reports, improve immune function, and give a sense of "well-being."...

Ngày tải lên: 10/08/2014, 08:20

... Bcl-XL, and Mcl-1) Bcl-2 was over-expressed in all fresh cases, and cell lines except the WSU-WM (expressed low levels), Bcl-XL was expressed in all patient cells and cell lines (except WSU-ALL cell ... throughout the experiment by cell count and viability every 24 hr for 72 hr using 0.4% trypan blue stain (Gibco BRL, Grand Island, NY) and a hemacytometer Fresh primary lymphoma cells isolated from patients ... of DNA fragmentation of extracts from both WSU-FSCCL and WSU-DLCL2 cells (Fig 3A.3 and 3B.2) Baseline expression of Bcl-2 family proteins in cell lines and fresh lymphoma cases To determine if...

Ngày tải lên: 10/08/2014, 22:20

... 299 cells (a human CD30-expressing ALCL cell line from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig) and Jurkat cells (a CD30-negative human leukemia/lymphoma cell ... 299 and Jurkat reporter cells that stably express eGFP and luciferase gene were utilized [43] The cells were treated with nanocomplexes containing the CD30 aptamer and eGFP siRNA for days, and ... 299 cells and Jurkat cells to the nanocomplexes had no effect on cell viability (Figure 6C) Moreover, cells were treated with the nanocomplexes for luciferase gene silencing as described above and...

Ngày tải lên: 11/08/2014, 00:22

... potentially aggressive and capable of multiple local recurrences and both locoregional and distant metastases Management of this type of tumor should include wide en bloc resection and longterm follow-up ... Histological view Histological view 10× H&E – proliferation of uniform and monomorphic cells with small nuclei and clear cytoplasm; no atypia and no mitosis Page of (page number not for citation purposes) ... Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA) for his efforts and support in the final histological analysis References In the maxillo-facial area, clear cell odontogenic...

Ngày tải lên: 11/08/2014, 21:22

... , a woman and her children saw house an egg-shaped object in one of his E- A farmer saw fields and also aliens collecting soil samples in 1964 claimed they were captured by aliens and F- In ... pairs and guess the answers What happened in 1947 ? a Kenneth Arnord saw nine large objects b Kenneth Arnord saw nine large objects The answer is: B When did a farmer see an egg-shaped object and ... Watch a video clip and guess what we will learn today! Unit 10 LIFE ON OTHER PLANETS Lesson GETTING STARTED & LISTEN AND READ Questions : What can you see in the pictures...

Ngày tải lên: 24/06/2013, 01:27

... inherit from a Visual Basic class and vice versa, and your program can be a mixture of C# and Visual Basic modules (and, for that matter, “managed” C++ and J# and ) No Global Data or Functions ... constructors and, 382–383 global data or functions and, 380 Index interface and, 383–384 memory allocation and, 380 multiple inheriting and, 383 overview of, 379–380 pointer variables and, 381 template ... destructor and, 271–272 HAS_A relationship and, 259–261 hierarchy, restarting, 296–299 importance of, 253–254 interface and, 316–319 invalid cast and, 262–263 is operator and, 263–264 IS_A property and, ...

Ngày tải lên: 04/10/2013, 21:20

... (O’Reilly) • Chapters and in Operating Systems: Design And Implementation, by Andrew S Tanenbaum and Albert S Woodhull (Prentice Hall) • Chapter in Modern Operating Systems, by Andrew S Tanenbaum ... its PV and NV fields (the string and floating-point represenations) and adjusted the FLAGS fields From this example you can clearly see that if you want your variables to stay shared and there ... chapter, when we will need to have a fair comparison between PerlHandler code and Apache::Registry scripts This will require both the code and the scripts to be preloaded at server startup Module Initialization...

Ngày tải lên: 26/01/2014, 07:20

... (RARa and b, RXRa and b) have a pSG5 backbone Cell culture and transient transfections Green monkey kidney cells (CV1), mouse embryonic carcinoma cells (P19) and human cervix carcinoma cells ... transcription in P19 cells by S-4o9cDH-RA P19 cells were simultaneously treated with and 10 lM of S-4o9cDH-RA and incubated for h (A) and 24 h (B) As a positive control for RARb2 induction, cells were ... at-RA and S-4o9cDH-RA for activating the retinoid receptors (Fig 2F) Hepa-1 cells were transfected with pGL3b-RARbluc and treated as the P19 cells in Fig 2D In contrast with the results in P19 cells,...

Ngày tải lên: 18/02/2014, 08:20

... VEGF-induced MAP kinase activation and cell proliferation [29] To distinguish between the extracellular effects of PF-4 acting on ligand/receptor activation and intracellular effects on signalling ... (Lariboisiere Hospital and Saint Isabelle Clinic) under approval, and with appropriate understanding and consent of the subjects DNA synthesis HUVEC were seeded at 20 000 cells per well in M199 ... antibody that recognizes both CXCR3A and CXCR3B, indicated that only 10% of HUVEC cells were positive (Fig 6B); all HUVEC cells expressed CD-31 (Fig 6B) Despite few cells expressing this receptor...

Ngày tải lên: 19/02/2014, 16:20

... order: m/z 181.2, 180.2, 182.2 and 178.2 The peak with m/z 179.2 provided an MS/MS spectrum showing fragments with m/z 162.2, 150.2 and 136.2 (not shown) HPLC separation and MS analysis of DAPY oxidation ... ESI-IT-MS as described in Materials and methods Three peaks at elution times 3.0 (1), 15.8 (2) and 23.0 (3) were collected and their composition analyzed using ESI-MS and MALDI-MS The largest peak ... triethylamine acetate, pH 7.0, and separated by RP-HPLC as described in Materials and methods The 310 nm-peak at an elution time 3.0 was collected and analyzed by ESI-IT-MS and MS/MS The spectrum shown...

Ngày tải lên: 19/02/2014, 16:20

... “Mr and Mrs Smith”, “Mr And Mrs Smith”, and so on To deal with these aliasing forms, we transform all different forms into a standard form for the later ranking and identification The standard ... retrieving candidates from returned snippets, combining both linguistic and statistical information to find the correct translation Our system can be split into three steps: candidate retrieving, candidate ... Yorker” and “紐約(pronounced as NiuYue)客(people, pronounced as Ke)” “橫濱” and “Yokohama”, “鈴木一朗” and “Ichiro Suzuki” “The Mask” and “ 摩 登 (modern) 大 (great) 聖 (saint)” “孫中山(Sun Zhong-Shan)” and “Sun...

Ngày tải lên: 20/02/2014, 12:20

... member p38 and has been shown to inhibit insulin induced glucose uptake by 3T3-L1 adipocytes and L6 muscle cells [35,36] Arsenite-treatment induced p38-phosphorylation on Thr180 and Tyr182, and phosphorylation ... activity and p38 MAPK activation) upstream of GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes Acknowledgements We thank Drs Hans Joost and Annette Schurmann (Aachen and ¨ Potsdam, Germany) ... this analysis, sonicated cells are probed with an antibody recognizing GLUT1 or GLUT4 and subjected to immunofluorescnce microscopy As can be seen in Fig 2E, both GLUT1 and GLUT4 are present in...

Ngày tải lên: 21/02/2014, 00:20

... specifically expressed in germ cells of both male and female oysters, and we hypothesized that Oyvlg had a role in germ cell formation [17] However, the function of Oyvlg in germline development had ... cells and the regression of gonadic tubules in the whole gonadic area (Fig 3) Our results demonstrate that Oyvlg has an essential role in germ cell (germ stem cells and gonia) proliferation, and ... mRNA, specifically expressed in auxiliary cells of the germ cells [27], were 0.54 ± 0.20 for controls, 0.69 ± 0.30 and 0.59 ± 0.17 for oysters injected with 20 and 100 lg of oyvl-dsRNA, respectively...

Ngày tải lên: 07/03/2014, 00:20

... faster and less labor intensive with proteins prepared by the cell- free approach than the cell- based approach (c) Proteins produced with the wheat germ extract from CellFree Sciences and labeled ... CESG’s wheat germ cell- free platform supports rapid and economical small scale screening for expression and solubility We currently test constructs with and without an N-terminal tag and have shown ... have found this technology to be robust, and our wheat germ cell- free pipeline now supports high-throughput screening for protein production and solubility and provides stable isotope labeled protein...

Ngày tải lên: 07/03/2014, 12:20

... mutant cells [27], wild-type CHO K1 cells, and mutant cell lines defective in the expression of heparansulfate (CHO 677 cells) or both heparan- and chondroitinsulfate proteoglycans (CHO 618 cells), ... CHO cell lines that bind to the cell surface In view of this, we investigated endocytosis of hLf in CHO cell lines, the wild-type K1 cells and the proteoglycan-deficient cell lines CHO 677 and ... mammary gland cells The binding of both hLf and anti-nucleolin polyclonal Ig to MDA-MB-231 cells was studied by fluorescence microscopy (Fig 8A) Interestingly, fluorescence staining revealed that both...

Ngày tải lên: 07/03/2014, 15:20

... HeLa cells and microsomal membranes of recombinant cells were prepared as described in Experimental procedures Microsomal proteins were subjected, or not, to Endo H and PGNase F digestion and ... subcellular localization (A) Microsomal membranes from cells expressing CD4–TMD ⁄ CT26 were treated, or not, with Endo H and PGNase F, and analysed by Western blot using anti-CD4 sera (B) Cells ... in Golgi and cell- surface expression of CD4–TMD chimera The concept that TMD length determines distribution between the Golgi and plasma membrane was initially reported for both Golgi and plasma...

Ngày tải lên: 07/03/2014, 16:20

... miR-133a and miR-133b inhibited cell Esophageal Cancer – Cell and Molecular Biology, Biomarkers, Nutrition and Treatment proliferation and invasion in ESCC MiR-200a has been linked to the etiology and ... resulting in cell morphology change and increased invasiveness [33] 24 Esophageal Cancer – Cell and Molecular Biology, Biomarkers, Nutrition and Treatment Fig Growth factors, Ras signaling and cell ... therapy [4] Growth factors regulate growth and development of cells They might be supplied by distant glands and tissues, neighboring cells, or in situ by tumor cells themselves Thus growth factors...

Ngày tải lên: 08/03/2014, 00:20