1 100 1 800 nm titan cutera

... al RT29 .1 Product RT25.5 10 0 50 Product RT30.7 200 15 0 17 (OH)D3 RT 31. 8 Product RT20.4 10 0 50 B E 23(OH)D3 200 15 0 10 0 15 0 10 0 50 C F I 20,23(OH) D3 200 Response (mV) RT33.2 15 0 10 0 50 17 ,20,23(OH) ... 17 ,20,23(OH) D3 15 0 10 20 30 Time (min) 40 50 80 60 40 200 RT25 .1 100 50 17 ,20(OH) D3 RT30.8 15 0 17 (OH)D3 RT 31. 9 10 0 50 0 17 (OH)D3 RT 31. 6 20 Response (mV) 0 10 0 RT29 .1 200 50 Response (mV) H 17 ,20(OH) ... run in 10 0% methanol at a sample flow rate of 30 lLÆmin )1 Nitrogen was used as both the bath (250 LÆh )1) and nebulizing gas (12 LÆh )1) The scan range for the mass spectrum was m ⁄ z 10 0 10 00 Acknowledgements...

Ngày tải lên: 18/02/2014, 17:20

... Al-Anizy et al 11 6 97 66 43 M B U P S 4x B M 10 10 Brain Liver α-mCyp4x1 10 10 µg Liver Brain pre-adsorbed C 30 10 0.5 0 .1 µg microsomes ng Cyp4x1 Fig Western blotting for mouse Cyp4x1 (A) Expression ... (19 97) A novel murine P-450 gene, Cyp4a14, is part of a cluster of FEBS Journal 273 (2006) 936–947 ª 2006 The Authors Journal compilation ª 2006 FEBS 945 Cyp4x1 in mouse brain 10 11 12 13 14 15 ... rabbit CYP4B1 (cyp4b1), rat CYP4B1 (cyp4b1) and human CYP4B1 (cyp4b1) The conserved region is underlined (B) Alignment of deduced amino acid sequence of rat, human and mouse CYP4x1 proteins 938...

Ngày tải lên: 19/02/2014, 07:20

... CYP6A2vSVL 0.03 0.04 0.05 0. 01 0.04 0.63 1. 00 1. 33 1. 66 0.33 1. 33 21. 00 1. 29 2.49 0.97 ND 2 .19 7.50 1. 00 1. 93 0.75 NC 1. 69 5. 81 2 .10 10 .06 3.27 2.82 4. 61 18.03 1. 00 4.79 1. 56 1. 34 2 .19 8.59 ± ± ± ± ± ± ... (3) (3) (3) (3) (3) ± ± ± ± ± ± 800 255 250 10 5 10 0 13 0 (16 ) (5) (6) (3) (11 )* (8)* * Statistically different from the reference (CYP6A2wt) (Dunnett test, P £ 0. 01) Ó FEBS 2004 DDT metabolism ... contained house fly cytochrome b5 (1 nmol) [42], house fly cytochrome P450-reductase (1 nmol) [8], CHAPS (0 .15 %), dilauroylphosphatidyl choline (1 mgÆmL )1) and 10 0 pmoles of cytochrome P450 In the...

Ngày tải lên: 19/02/2014, 12:20

... excluding the partici- 1. 396 (1. 3 71) {1. 392} 1. 897 (2.039) {1. 898} 1. 743 (1. 716 ) {1. 750} 1. 463 {1. 485} 1. 462 {1. 499} 2. 412 (2.5 01) {2. 413 } 1. 944 {1. 882} 4TS1-IV (2TS1-IV) {4TS1-III} 2.465 {2.523} ... superoxo-ferrous state 15 6 15 7 15 8 15 9 16 0 16 1 16 2 16 3 16 4 16 5 16 6 16 7 16 8 16 9 17 0 17 1 17 2 in a reduced oxy-ferrous hemoprotein and model compounds J Am Chem Soc 12 5, 16 34 016 346 Leibl, W., Nitschke, ... Shiro, Y (2002) Enzymatic 4354 P Hlavica (Eur J Biochem 2 71) 10 3 10 4 10 5 10 6 10 7 10 8 10 9 11 0 11 1 11 2 11 3 11 4 11 5 11 6 11 7 11 8 reaction of hydrogen peroxide-dependent peroxygenase cytochrome P450s: kinetic...

Ngày tải lên: 19/02/2014, 16:20

... Radiolabeled [1- 14C]lauric acid (1 6 MBqÆlmol )1) was from CEA (Gif sur Yvette, France); [1- 14C]oleic acid (1 85 MBqÆlmol )1) , [1- 14C]linoleic acid (2 1 MBqÆlmol )1) and [1- 14C]a-linolenic acid (1 9 MBqÆlmol )1) ... Km,app and Vmax,app values of 17 2 ± 13 lm and 11 7 ± nmolÆmin )1 nmol )1 P450, respectively (Fig S3) The x -1 position of lauric acid corresponds to carbon 11 of physiological C18 fatty acids, closely ... gÆL )1; sucrose, 10 gÆL )1; pastagar B, gÆL )1; myoinositol, 10 0 mgÆL )1; thiamine, 10 mgÆL )1; nicotinic acid, mgÆL )1; pyridoxine, mgÆL )1; final pH 5.7) for weeks Arabidopsis plants (approximately 10 ...

Ngày tải lên: 07/03/2014, 03:20

... )2 51 E3 )288 )283 ) 310 )328 )3 31 )328 )366 )336 )380 31 10 10 26 31 E2 [FMNhqFADox] [FMNsqFADsq] E4 5 3 11 )388 )382 )403 )3 81 )342 ) 417 )385 )462 ) 419 11 10 6 11 1. 7 ... electrons to NADP+: eẳ a 10 EE1 ị=59 ỵ b ỵ c 10 E2 Eị=59 ỵ 10 EE1 ị=59 ỵ 10 E2 Eị=59 d 10 EE3 ị=59 ỵ e ỵ f 10 E4 Eị=59 ỵ ỵ 10 EE3 ị=59 ỵ 10 E4 Eị=59 g ỵ h 10 EE5 ị=29:5 ỵ ỵ 10 EE5 ị=29:5 9ị where g ... unit )1, for a 1- electron 1- proton process [4446] However, all four slopes were smaller than )59 mV, namely )43 mVặpH )1 (E1), )17 18 mVặpH )1 (E2), )32 mVặpH )1 (E3) and )47 10 mVặpH )1 (E4)...

Ngày tải lên: 07/03/2014, 06:20

... (mM) 0 .1 P450nor Overall activity (%) kobs (s )1) kdec (s )1) Wild-type D88A D88V D88N D393A 10 0 25.5 ± 1. 5 22.5 ± 0.5 36.5 ± 1. 5 10 .0 45 ± 41 ± 99 ± 16 ± ND 0.027 0 .10 0.089 0.077 ND 0.5 0 .1 kobs ... 0.089 0.077 ND 0.5 0 .1 kobs (s )1) kobs (s )1) kdec (s )1) kobs (s )1) kdec (s )1) ND 1. 6 ± 0.2 7.3 ± 0.5 ND ND 0 .10 0 .19 ND ND 7.6 ± 0.8 22 ± 2.9 ± 0.5 ND 0 .10 0 .11 ND 8.7 1. 0 Ó FEBS 2004 Asp88 in haem-distal ... kinetics of ferric-NO 11 12 13 14 15 16 17 18 19 20 complexes of wild-type and mutant enzymes J Biol Chem 275, 4 816 –4826 Kudo, T., Takaya, N., Park, S.-Y., Shiro, Y & Shoun, H (20 01) A positively charged...

Ngày tải lên: 07/03/2014, 15:20

... 13 C d (p.p.m.) 1a 1b 2a 2b 6a 6b 7a 7b 10 11 a 11 b 12 12 b 13 14 15 a 15 b 16 a 16 b 17 18 19 3.95 – 2.46 2.53 N/A 5.75 N/A 2. 31 2.54 1. 00 1. 90 1. 69 1. 15 N/A 2.07 1. 55 1. 09 1. 84 N/A 0.98 1. 60 1. 31 ... 37.8 37.8 – 51. 65 24 .1 24 .1 30.2 30.2 82.2 11 .4 13 .25 2. 31 1.48 4 .11 N/A N/A 5.70 N/A 2.20 2. 51 1.09 1. 77 1. 90 1. 39 N/A 1. 71 1 .15 0.94 1. 92 N/A 0.96 1. 51 1.27 1. 88 1. 36 3.49 0. 71 1 .15 40.46 40.46 ... of 10 0 lM Ô–Õ Indicates rate < 0 .1 min )1 Rate (min )1) P450 1b-OH 2b-OH 6b-OH 1A1 1A2 1B1 2C9 2D6 3A4 – – – – – 4.8 ± 0 .1 – – – – 0. 31 ± 0. 01 11 ± 0 .1 1 .1 6.7 7.7 0.93 0. 91 83 ± ± ± ± ± ± 15 b-OH...

Ngày tải lên: 07/03/2014, 16:20

... NHis-Fdmor CHis-Fdmor Purified ferredoxin (nmolÆL )1 culture) A280 ⁄ A 412 Predicted mass (Da) Estimated mass (Da) 60 14 0 210 1. 79 6793 6795 2.35 8820 8824 1. 62 8 313 8 314 11 49 Studies on the mycobacterial ... Milton Keynes, UK) NBT reduction was measured at 535 nm (e535 ¼ 18 300 m )1 cm )1) and cytochrome c reduction at 550 nm (e550 ¼ 21 100 m )1 cm )1) Reactions were performed in 50 mm glycine buffer, ... (electronsÆmin )1) Km (nM) Vmax ⁄ Km (electronsÆmin )1 nM )1) wt-Fdmor NHis-Fdmor CHis-Fdmor 887 ± 952 ± 60a 807 ± 26a 5.3 ± 0.3 10 .5 ± 1. 9a 3.7 ± 0.5a 16 7 91 218 a Values obtained by fitting data to a modified...

Ngày tải lên: 07/03/2014, 16:20

... (CYP11A1) Proc Natl Acad Sci USA 10 0, 14 754 14 759 19 Tuckey RC & Cameron KJ (19 93) Side-chain specificities of human and bovine cytochromes P-450scc Eur J Biochem 217 , 209– 215 20 Tuckey RC (19 92) ... a 11 00 LC capillary equipped with Zorbax SDC18 column (15 0 · 2 .1 mm; 3.5 lm particle size) coupled with the 11 00 LC ⁄ MSD-Trap-XCT system Separation was performed at flow rate of 15 0 lLÆmin )1 ... Biophys Acta 11 63, 18 5 19 4 25 Toaff ME, Schleyer H, Strauss JF & 3rd (19 82) Metabolism of 25-hydroxycholesterol by rat luteal mitochondria and dispersed cells Endocrinology 11 1, 17 85 17 90 26 Hanukoglu...

Ngày tải lên: 07/03/2014, 21:20

... Pregnenolone Species Km (lM) V (nmolÆh )1 mg protein )1) Km (lM) V (nmolÆh )1 mg protein )1) Baboon Human 6.5 ± 0 .1 0.8 ± 0 .1 3.9 0.5 0.9 ± 0.05 1. 3 ± 0.2 0.45 0.5 5 614 A C Swart et al (Eur J Biochem ... 84, 407– 411 12 Zuber, M.X., Simpson, E.R & Waterman, M.R (19 86) Expression of bovine 17 alpha-hydroxylase cytochrome P-450 cDNA in nonsteroidogenic (COS1) cells Science 234, 12 58– 12 61 13 Tremblay, ... steroid -17 ,20-lyase reactions Mol Endocrinol 3, 968–975 15 Zang, P., Nason, T.F., Han, X.G & Hall, P.F (19 92) Gene for 17 alpha-hydroxylase/C (17 –20) lyase P-450: complete nucleotide 16 17 18 19 20 21...

Ngày tải lên: 08/03/2014, 09:20

... 96.5 1R-Camphor a – – )19 .6 ± 0.9 )13 .2 ± 0.8 14 15 15 4 13 11 10.2 ± 2 .1 7.6 ± 2.0 8.4 ± 0.8 )18 .2 ± 3.8 )13 8.8 ± 27.8 4.6 ± 2.0 – – )10 .6 ± 1. 1 )53 .1 ± 21. 9 35 11 40 206 12 14 208 14 18 11 11 50 11 50 14 90 ... 15 8 .1 (54%; 19 41. 1 cm )1) & 13 2.5 (8%; 19 51. 9 cm )1) & 3 81. 3 ( 31% ; 19 60 .1 cm )1) 332 343.8 (19 53.3 cm )1) 3 81 1000 340.8 (19 46 .1 cm )1) 13 00 )1 )1 M Water influx rate DHS%/ (K 10 min) [3] ba (GPa )1) ... (GPa )1) [7] )19 .6 )13 .2 ) 31. 0 – )10 .6 & )18 .2 – 0 .14 7 0.00 713 0.302 0.00638 )32.0 )14 .0 )20.0 – – – 0.009 81 – 0. 012 71 4.6 & 10 .2 – – 0. 012 28 8.4 – 7.6 3.0 – 7.0 0.538 – 0.5 71 0. 014 45 – 0. 011 3 DV# (cm3Æmol )1) ...

Ngày tải lên: 08/03/2014, 23:20

... at concentration of 10 0 lM in all experiments b D V 8 .1 8 .1 7.8 7 .1 7.3 V b (nmol product ⁄ nmol P450 min )1) 0. 018 0. 019 0 .17 0 .17 0.077 0 .11 0 .12 0 .14 0.093 0 .14 D V 0.95 1. 0 0.70 0.86 0.66 Results ... Sample code no 10 4 10 9 11 0 11 5 13 2 a 7-OEt coumarin substrate d0 d2 d0 d2 d0 d2 d0 d2 d0 d2 a V b (nmol product ⁄ nmol P450 min )1) 0.048 0.0059 0.25 0. 31 0 .14 0 .18 0 .17 0.024 0 .19 0.026 Used at ... 2.4 11 ± 6.7 ± 1. 7 0.036 ± 0.009 14 ± 5.0 ± 0.7 0.82 ± 0 .12 10 ± 6±2 13 ± 3.0 ± 1. 0 0 .13 ± 0.04 9.0 ± 1. 0 FEBS Journal 273 (2006) 2223–22 31 ª 2006 The Authors Journal compilation ª 2006 FEBS 11 ...

Ngày tải lên: 16/03/2014, 13:20

... 1A, the transformation was measured over time after treatment with three different E doses: (a) 1. 9 pmol E 10 0 mg calli )1, which does not promote P450 induction; (b) 8.6 nmol E 10 0 mg calli )1, ... P450 inhibitors tested show also a strong inhibitory effect B 0. 016 0. 015 0. 014 Abs 42 3nm 0. 013 0. 012 0. 011 400 450 500 Wavelength (nm) Fig Isolated P450 in P vulgare (A) SDS ⁄ PAGE of cytochrome ... culture Radioactive E (1. 9 pmol [23,24-3H]E 52.2 CiÆmmol )1, 8.5 nmol [23,24-3H]E 11 .5 mCiÆmmol )1 or 86 nmol [23,24-3H]E 1. 15 mCiÆmmol )1) was applied in lL of H2O ⁄ MeOH (v ⁄ v) : solution At...

Ngày tải lên: 16/03/2014, 23:20

... genes Faseb J 10 , 11 12 11 17 Waxman, D.J (19 99) P450 gene induction by structurally diverse xenochemicals: central role of nuclear receptors CAR, PXR, PPAR Arch Biochem Biophys 369, 11 –23 Pereira, ... expression of the CYP3A genes 10 11 12 13 14 15 Acknowledgements Supported by Fundacao para a Ciencia e Tecnologia – grant PRAXIS ¸ ˜ ˆ 4/4 .1/ BD/2999 (E.R); project PRAXIS XXI 2/2 .1/ BIA/234/94 – and by ... approximately 800 bp of the 5¢ upstream region of the CYP3A1 locus (Fig 1B) Deletion of the DNA segment )16 6 to ) 811 severely reduced transactivation by C/EBPa Moreover, transactivation of the p0.8T81Luc...

Ngày tải lên: 17/03/2014, 09:20

... ± 10 .2 ± 4.8 – 0.09 ± 0.02 11 .3 ± 1. 5 1. 1 ± 0.2 10 0 ± 1. 0 0.2 ± 0.3 45 ± 9.0 3.9 ± 0.3 >10 0 7.5 ± 1. 0 10 6 ± 3.0 1. 0 ± 0.5 10 0 ± 5.0 16 6 ± 5.9 10 7 ± 4.9 10 1 ± 7.2 (SRS 3) I371K 53.2 ± 7 .1 7 .1 ... 0.7 >10 0 12 0 ± 9.9 10 3 ± 2.2 35 ± 3.8 23 ± 9.2 n.m 10 0 ± 5.0 91. 1 ± 21 136 ± 14 11 9 ± 17 83 ± 1. 7 10 0 ± 13 (SRS 6) R368F 81 ± 3.3 – K484M 54 ± 4.3 – 89 ± 1. 4 – 2 2 30.7 ± 0.6 13 ± 1. 2 – 60 ... ± 1. 7 7.8 ± 0.2 11 .6 ± 0.8 10 0...

Ngày tải lên: 17/03/2014, 10:20

... CH2O 3 81 ( )18 ) H2O 355 ()44) CH2NO 289 ( )11 0) 2 71 ( )12 8) 411 ( )18 ) 3 81 ()30, )18 ) 311 ( )11 8) 435, 437 ()30) 399 ()30, )36) 459 ()30) 4 41 ()30, )18 ) 429 ()60) 399 ()60, )30) 356 ()57) 413 M + ... Salmonella typhimurium III FEBS Journal 278 (2 011 ) 216 7 217 8 ê 2 011 The Authors Journal compilation ê 2 011 FEBS L Perrin et al 12 13 14 15 16 17 18 19 20 21 22 23 Methylated derivatives of 9H-carbazole ... of kinetic isotope effect proles J Phys Chem B 11 1, 77007 710 FEBS Journal 278 (2 011 ) 216 7 217 8 ê 2 011 The Authors Journal compilation ê 2 011 FEBS 217 7 Formation and characterization of stable carbinol-amide...

Ngày tải lên: 22/03/2014, 16:20

... Flowering? [13 ] [15 ] [22] [18 ] [14 ] [24] [23] [34] [30] [29] [26] [20] [37] [38] [39] [19 ] [ 31] [ 21] [34] [54] [55] 19 6 FEBS Journal 278 (2 011 ) 19 5–205 ª 2 010 The Authors Journal compilation ª 2 010 FEBS ... Enzymatic activity Physiological role Ref 94A1 94A2 94A5 94C1 86A1 86A2 86A8 86A4 86A22 86A33 86B1 81B1 703A2 704B1 704B2 709C1 726 A1 77A4 77A6 92B1 78A1 Vicia sativa Vicia sativa Nicitiana tabacum ... site This is the case for CYP94A1 [17 ], CYP94C1 [18 ] and CYP709C1 [19 ] which metabolize in vitro 9 ,10 -epoxystearic acid with a higher efficiency than C18 :1, C18:2 or C18:3 This suggests a strong interaction...

Ngày tải lên: 22/03/2014, 16:21

... Yeast 18 , 11 17 11 29 30 Broadway NM, Dickinson FM & Ratledge C (19 93) The enzymology of dicarboxylic-acid formation by Corynebacterium sp strain 7e1c grown on n-alkanes J Gen Microbiol 13 9, 13 37 13 44 ... assigned CYP5025A1 and CYP5030A1, respectively No P450 activity is linked to a-hydroxylation; this FEBS Journal 278 (2 011 ) 206–2 21 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 211 The role of ... Five genes were clearly induced by octadecane (CYP52A12, -13 , -14 , -17 and -18 ), whereas no transcription was detected for CYP52A15, -16 , -19 , -20 and D2 under those and other conditions Also,...

Ngày tải lên: 22/03/2014, 16:21