1 the problems for large ex vivo expansion of hscs

... purposes) Journal of Translational Medicine 2009, 7:82 3a-G1 inhibits IL-2 related expansion of CD4+/CD25+/ CD127-/FoxP3+ regulatory T cells IL-2 is critical for the ex- vivo expansion of suppressive ... such expanded NK cells, we had to decipher the origin of this expansion process Here, we show that 3a-G1 driven expansion of NK cells from PBMCs is not occurring through a direct activation of ... the proliferation and the expansion of T cells was maintained in the presence or absence of exogenous IL-2 (Fig 3b) Thus, monocytes are involved in the ex- vivo priming of autologous CD4+T cells...

Ngày tải lên: 18/06/2014, 15:20

... array of implementation options might force you to make some trade-offs For example, you might choose to trade-off ease of implementation for performance, or to trade-off richness of services for ... describe the problems in using binary protocols and explain how the adoption of Web standards alleviates many of these problems ! Problems with Binary Protocols ! Web Protocols and Data Formats Lead-in ... server failure ! Failure of an action because a required resource (for example, database connections) had been exhausted ! The need for excessive hardware than is required for typical loads, just...

Ngày tải lên: 10/12/2013, 16:15

... consistently positive for HIV RNA [15] However, for obvious clinical and ethical reasons, in-depth investigations of HIV infection of the prostate of HIV+ asymptomatic men have never been performed In vitro ... surface of the tissue section in a minimum of three independent cultures using the Cast Grid software (Olympus) Infection of prostate explants Immediately after dissection, two fragments of human ... 17 days of culture The overall morphology of the organ was preserved after 17 days of culture despite a loss of stromal cells in some areas (F) The markers used for the characterization of prostatic...

Ngày tải lên: 13/08/2014, 05:21

... control mouse Ex vivo Ex vivo bioluminescence imaging and histological analysis of a large HSV-TK-Luc tagged U87MG glioma in a control mouse (A) Bioluminescence image (exposure time 10 min) of the freshly ... stably expressing the HSVTK-Luc fusion construct Correlation of HSV-TK with luciferase activity in vivo The above high expresser U87MG cell clone was used for xenograft experiments in nude mice For ... therapeutic transgenes to tumors relies on ex vivo analysis of explanted tumor tissue [7,9,10] Time course analysis of transgene expression thus requires a multitude of animals to be sacrificed In the...

Ngày tải lên: 14/08/2014, 19:22

... and, with the exception of LAW34-L for CrFK cells, had reduced transduction efficiencies (Fig 4C) As a result of these studies, LAW34 was selected for the experiments below Effect of transduction ... but this extended host range can be unpractical for vector usage in vivo where restricted cell targeting is often required Moreover, VSV-G pseudotyped vectors have proved toxic for ex vivo cells ... part of gag, form the ψ signal required for viral RNA encapsidation into assembling virions and were deleted of Figure Safety evaluation of packaging and vector constructs Safety evaluation of...

Ngày tải lên: 14/08/2014, 19:22

Ngày tải lên: 16/03/2014, 03:20

... BtR175 of B mori Indeed, improvement of these loops by mutation has been performed for several Cry toxins For example, loop mutants of Cry1Ab and loop mutants of Cry3Aa have higher insecticidal ... cloned into a GST-tagged expression vector, pGEX-4T3 (GE Healthcare, Little Chalfont, UK), and used for the transformation of the BL21 strain of E coli Each mutant toxin was expressed, activated ... affinity of the BtR175 fragment as a result of steric hindrance Therefore, we next analyzed the effects of DF and IAF modifications, which show greater and smaller hindrance, respectively, for the...

Ngày tải lên: 29/03/2014, 22:21

... of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments RV and SP: protocol design and obtention of the consent ... (inverse of erythrocyte deformability) [20], defined as: RI = ( Tb − Ts) / ( Ts) × 100 / Htc Where: Tb: time of passage of the cell suspension through the filter; Ts: time of passage of an equal ... C.I.95%: confidence interval (n = 6) Degree of significance of the difference between groups: * p < 0.05 Ex vivo experiments Previous analysis [16] performed on erythrocytes from active RA patients...

Ngày tải lên: 10/08/2014, 05:21

... Phenotype of day-14 ex vivo expansion cultures: Liquid cultures for 14 day ex vivo expansion were established with 100,000 CD34+ cells in 50 ng/ml of SCF, TPO and Flt-3 Ligand The results of six ... clinical-scale transplantation using ex vivo CB products To overcome this problem, new approaches for ex vivo expansion need to be developed, aiming at expansion of the "stem" cell population in ... days of culture, cells were harvested to analyze CD34 and CD52 expression profiles A: Expression profiles of CD34 and CD52 at Day and Day 14 of secondary cultures B: Absolute expansion fold of...

Ngày tải lên: 10/08/2014, 22:20

... assessment of the biological activity of this extract in vivo and on the chemical identification of the active components responsible for the anti-inflammatory activity The knowledge of the role of flavonoùds ... department of botany of the national institute of agronomy in Algeria Flavonoùds were extracted according to the extraction method described previously by Paris and Nothis [36] Briefly, 20g of the ... quantify supernatants levels of IL-12 and IL-4 The lowest level of sensitivity was 10 pg/mL for IL-12 and pg/mL for IL-4 of the cytokine NO production by PBMCs PBMCs of patients and NCs were cultured...

Ngày tải lên: 11/08/2014, 03:20

... and expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1 IFN-gR KO mice was flushed out of ... increasing numbers of MSCs is shown Each result represents the mean of four cultures ± standard error of the mean (SEM) Results are representative of two independent experiments * P < 0.05 for comparison ... performed as described previously [32] Bio-Plex protein array system Expression of cytokines (that is, interleukin-2 [IL-2], IL5, IL-6, IL-10, IL-17, and IFN-g) was determined by the Bio-Plex...

Ngày tải lên: 12/08/2014, 11:23

... levels of viral excretion confirming the virulence of the viruses used Individual susceptibility or technical problems during the viral inoculation could account for the lack of infection of the ... viral titres observed in the extracellular fraction of the one-step In order to perform an in vivo comparative study of the reference BoHV-5 strains, two groups of calves were infected with N569 ... particles in nasal swabs, one of them showing an intermittent profile of viral excretion (Table 1) All the animals infected with A663 strain shed virus, three of them excreted during 5-13 days and...

Ngày tải lên: 12/08/2014, 18:22

... Micro-environment 35 2.4 Analysis of product positioning strategy of Bravia LCD TV: .41 2.5 Analysis of current situation of Marketing-mix for Bravia LCD TV Series of Sony Vietnam: .46 ... environments (internal and external of Sony Vietnam) Methods of study and process of data - Methods of study: quantitative and qualitative study in combination with expert method, summary, statistics, ... Analysis of Marketing environment and current situation of Marketing-mix for Bravia LCD TV Series of Sony Vietnam Chapter 3: Some solutions to the Marketing-mix for Bravia LCD TV Series of Sony...

Ngày tải lên: 19/05/2015, 15:57

... provide a brief overview of the concepts of ethnicity, ethnic identity and the process of ethnic formation Two main perspectives for the explanation of the phenomenon of group ethnic identity ... context of post-socialist economic reform This dissertation examines the Buluotuo cultural tourism development of the Zhuang in Tianyang County of Guangxi Zhuang Autonomous Region Based on extensive ... recognized officially as the largest of the 55 minority nationalities (shaoshu minzu)3 of the PRC Most of them are concentrated in the western part of Guangxi, inhabiting the area south of the five...

Ngày tải lên: 09/09/2015, 08:16

... then assessed for cell viability Extracel is intended to be used as a cell carrier for seeding cells into the scaffolds for in vivo experiments 2.5.1 Assessment of cell viability in ExtracelTM 2.5.1.1 ... individual the amount of bone lost is balanced by the amount of bone formed However, in case of certain diseases and with age, bone resorption exceeds formation resulting in overall loss of bone Also ... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells...

Ngày tải lên: 09/10/2015, 11:24

... with SLE since production of IL-2 and the active form of TGF-β is decreased [53] Figure The role of transforming growth factor-β (TGF-β) in the differentiation pathway of CD4+ regulatory T cells ... regulatory T cells generated ex vivo with TGF-β can have protective effects in vivo For this purpose, we selected a mouse model of SLE that has a rapid onset The transfer of parental T cells to F1 ... inflammatory bowel disease or certain forms of multiple sclerosis The adoptive transfer of regulatory T cells generated ex vivo also has the potential to prevent the rejection of allogeneic organ transplants...

Ngày tải lên: 09/08/2014, 03:24

... agents is ongoing and is largely driven by issues relating to drug resistance, formulation of drug combinations, pharmacokinetic profiles and toxicity For example, combinations of nucleoside reverse ... showed extensive cell death at 52 and 103 μM WR1065, we chose to perform subsequent experiments at ≤ 26 μM WR1065 For the Annexin V assay, drug-exposed cultures ranged from 75% to 100% Annexin ... (non-apoptotic), with the majority of experiments showing 85-95% of the cells as Annexin-V negative (data not shown) Table also presents mean values for replicate experiments in which we exposed HIV-1-infected...

Ngày tải lên: 10/08/2014, 05:21

... carcinomas The maintenance of the vitality of these tissue samples is crucial for ex vivo cultivation As a consequence of extensive testing the culture period did not exceed 16 h, limiting also ... Effects of short-term cultivation upon expression of Ki-67 antigen and of cleaved caspase-3 expression in human NSCLC specimens of both adenocarcinoma and squamous cell carcinoma type Data are expressed ... enhancement of expression of caspase-3 after cultivation that was evident in 73% (11/15) of those tissue samples with low levels of this protein Two samples of the tested 17 SCC specimens, however, exhibited...

Ngày tải lên: 12/08/2014, 15:20

... Page of 13 Figure Mutation of S52A impairs Vpu-mediated degradation of CD4 and tetherin (A) Western blot analysis of Vpu expression in lysates of transfected 293T cells (B) FACS analysis of CD4 expression ... efficient release of HIV-1 particles in the presence and absence of Vpu did not express detectable levels of tetherin (Fig 2B) Of note, macrophages expressed markedly higher levels of tetherin than ... (Fig 2A and 2B) As expected, 293T cells expressing very low 10 ng (1:500) levels of tetherin released p24 independently of functional Vpu expression However, transfection of 20 ng (1:250) tetherin...

Ngày tải lên: 12/08/2014, 23:22