1 modulation of cell signalling

... mediated cell death Of note, apoptosis of fibroblast-like cells is regulated at a number of different levels, and there is evidence that secondary modulation of signalling pathways downstream of Fas ... significantly the susceptibility of cells to Fas induced cell death [17] However, the expression of Fas receptor on the cell surface together with the levels of soluble Fas are important factors ... addition to signalling through the death domain of TNFRI, TNF-α activates mainly signalling pathways and transcription factors such as nuclear factor-κB, which mediate the survival of cells Thus,...

Ngày tải lên: 09/08/2014, 01:23

... purines on cell growth 38 Table Strategies of the extracellular mechanism studies of AIA in BHK 184 cells Table Strategies of the extracellular mechanism studies of AIA in HeLa 185 cells xv ABBREVIATIONS ... Effect of NBTI on AIA in SKW6.4 cells 123 Fig 3.40 Effect of dipyridamole on AIA in SKW6.4 cells 125 Fig 3.41 Effect of NBTI on AIA in H9 cells 127 Fig 3.42 Effect of dipyridamole on AIA in H9 cells ... effects of adenosine on HeLa cells 66 Fig 3.4 Apoptotic effects of adenosine on SKW6.4 and H9 cells 67 Fig 3.5 Apoptotic effects of adenosine on SY5Y and MN9D cells 69 Fig 3.6 Apoptotic effects of...

Ngày tải lên: 11/09/2015, 16:07

... and YN-1 erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells, and HMV-II melanoma cells H146 small cell lung cancer cells were obtained from ... measurement of heme content is useful for evaluation of heme dynamics in cultured cells Role of heme metabolism in cellular heme content Regulatory role of free heme in expression of HO-1 To evaluate ... expression levels of HO-1 and HO-2 and cellular heme content The cellular heme content represents the sum of free heme and bound heme of various hemoproteins for maintenance of cellular heme contents...

Ngày tải lên: 19/02/2014, 05:20

... IL-1b-treated cells (C,D) n-3 PUFAs inhibit cyclin D1 expression and phosphorylation of Rb Lysates of whole cells were prepared from cells treated as described above and 20 lg aliquots of protein ... cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the earliest effects of mitogenic factors leading to cell cycle re-entry, G1-phase progression, ... level above that of control cells As expected, SMC stimulated with 10% (v/v) fetal bovine serum also had a reduced total content of caveolin-1 The concentration of the caveolin-3 isoform, mainly...

Ngày tải lên: 19/02/2014, 16:20

... described [21] However for easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein or with ... translocation of the full-length Tat protein and cellular uptake of its basic domain make use of the same mechanism Both genetic and biological evidence indicates that the cellular uptake of the Tat ... speci®c as a large number of cell lines tested so far entrapped the translocating peptide Fig In¯uence of temperature on the uptake of Tat CPP and of Tat±GFP fusion protein HeLa cells were incubated...

Ngày tải lên: 21/02/2014, 03:20

... the functional activities of GRIP1 They extend our understanding of the importance of the structural status of GRIP1 in modulating NR functions Results Autoregulation of GRIP1 AD activities by ... determination of total protein levels of Gal4DBD- or HA-GRIP1 fragments, aliquots of cell lysates were subjected to direct immunoblots Immunoblots were performed as previously described [23] using 10% of ... histogram with histogram of Fig 1B) Similar patterns of transactivation activity were exhibited by these GRIP1 fragments in human embryonic kidney 293 cells, and the identities of AD1, AD2, and at...

Ngày tải lên: 07/03/2014, 12:20

... from cells from the nondiabetic control subjects Similarly, the maximal effects of insulin on the state of tyrosinephosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ERK1 ... target cells of the hormone Studies of cells from patients with the disease and nondiabetic subjects have demonstrated that mutations in the insulin receptor cannot explain the vast majority of cases ... that a node of cross-talk between the stress-generated signal and insulin signalling is located at the level of IRS1 or between IRS1 and PKB The effect and ultimate function of stress signalling...

Ngày tải lên: 07/03/2014, 16:20

... energy of RT dimer dissociation is decreased in the presence of increasing concentrations of TSAOe3T, resulting in loss of dimer stability of 4.0 and 3.2 kcalÆmol)1 for p66/p51 and p66/p66 forms of ... the mechanism of inhibition of subunit association in vitro is different from the process in HIV-1 infected cells In HIV-infected cells the RT polypeptides are translated as part of the Gag–Pol ... permitting the formation of a pseudo antiparallel b sheet with one of the PR subunits The second class of molecules that inhibited PR dimerization include pentaester derivatives of of didemnaketal A...

Ngày tải lên: 08/03/2014, 09:20

... of cell shape and mobility  ancor for other cellular structures Presentation: DNA Cell Cycle and Cell Division Some Eukaryotic Cell- Cycle Times Cell Type Cell- Cycle Times Early frog embryo cells ... EUKARYOTES PROKARYOTES All organisms: or more cells The animal cell A plant cell A bacterium The Main Functions of the Membrane-bounded Compartments of a Eukaryotic Cell Compartment Main Function Cytosol ... Lesson 1: Basics of molecular biology I The Cell s Organization II Cell Cycle and Cell Division III Cellular Molecules IV The Genetic Dogma The Cell s Organization EUKARYOTES PROKARYOTES...

Ngày tải lên: 13/03/2014, 19:46

... of cell shape and mobility  ancor for other cellular structures Presentation: DNA Cell Cycle and Cell Division Some Eukaryotic Cell- Cycle Times Cell Type Cell- Cycle Times Early frog embryo cells ... EUKARYOTES PROKARYOTES All organisms: or more cells The animal cell A plant cell A bacterium The Main Functions of the Membrane-bounded Compartments of a Eukaryotic Cell Compartment Main Function Cytosol ... Lesson 1: Basics of molecular biology I The Cell s Organization II Cell Cycle and Cell Division III Cellular Molecules IV The Genetic Dogma The Cell s Organization EUKARYOTES PROKARYOTES...

Ngày tải lên: 13/03/2014, 19:47

... functional characterization of GPCRs is the production of sufficient amounts of protein sample Although considerable improvements have been made, the overproduction of GPCRs in cellular expression systems ... presence of Brij58 Lane 3: supernatant of ETBcHx expression in the presence of Brij78 Lane 4: ETBcHx after Ni–chelate acid chromatography Lane 5: supernatant of ETBStrep expression in the presence of ... after conventional expression in insect cells Ligand binding of cell- free endothelin B receptor A Single-particle analysis of CF-produced ETBcHx The quality of CF-expressed and purified ETBcHx was...

Ngày tải lên: 16/03/2014, 10:20

... implications of this subfamily of receptors in cellular signalling make them attractive therapeutic targets The coupling between stimulation of the P2X receptor and the generation of intracellular signalling ... is very often the first step in the biochemical analysis of the role of these domains in cellular signalling After disruption of cells, lipid rafts ⁄ caveolae can be extracted by virtue of their ... the effect of lipid raft disassembly in signalling functions of intact cells Lipid rafts in cellular signalling One crucial role attributed to lipid rafts is their ability to organize signalling...

Ngày tải lên: 30/03/2014, 02:20

... chemiluminescence Suppression of PEP expression in U343 cells A cell line with sufficiently high concentrations of PEP was required to investigate the cellular role of PEP The astroglioma cell line U343 showed ... activity of PEP in U343 cells Fmoc-Ala-PyrrCN is a potent and specific inhibitor of PEP [29] with a Ki of 70 pM against recombinant human PEP (data not shown) This inhibitor is able to penetrate the cell ... characterize the intracellular function of PEP, Ins(1,4,5)P3 concentration in the antisense cell lines was measured In U343 wild-type cells, it was  0.26 ± 0.02 pmol per 106 cells (n ¼ 4) It was...

Ngày tải lên: 31/03/2014, 08:20

... the differentiation of renal tubules representing the origin of clear cell renal cell carcinoma (RCC) and in the regulation of the cell cycle of parietal epithelial cells of the Bowman's capsule ... pathway plays an important role in distinct cellular processes such as cell proliferation, cell cycle, apoptosis and intracellular signalling [8], which are often disturbed in cancers [44,45] UCHL1 ... silencing of UCHL1 in RCC cell lines Methylation of UCHL1 in human primary RCC lesions, but not of corresponding normal kidney epithelium Since an impaired UCHL1 expression was not only found in RCC cell...

Ngày tải lên: 18/06/2014, 15:20

... media of culture passages five to seven of primary neural stem cells from cortices of E16 Sprague-Dawley rat embryos in three separate experiments A preponderance of NGF in pg per day of culture of ... foot faults (DOFFF) is the difference between the number of faults for the right and left forelimbs SOFFF and DOFFF were used as an index of motor deficit CSF aspiration 80 μL of CSF were aspirated ... number of foot faults in the left and right forelimbs The sum of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, whereas the differentiation of...

Ngày tải lên: 18/06/2014, 16:20

... concentration of viral pro- Figure of hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells ... and re-appearance of intracellular viral pro- Figure Confocal laser scanning image of intracellular RSV proteins Confocal laser scanning image of intracellular RSV proteins HEp-2 cells had been ... viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial cells HEp-2 cells had been infected at m.o.i of 50 for 12 h then incubated with anti-RSV...

Ngày tải lên: 18/06/2014, 18:20

... IM28 on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) and ... fusion of 293/CD4+ cells with TF228.1.16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228.1.16 cells (data not shown) The fusion of TF228.1.16 cells ... dexamethasone (B) cells are mainly exploded vs in the presence of IM28 Figure dexamethasone on fusion of TF228.1.16 cells with SupT1 cells Effect of2 Effect of dexamethasone on fusion of TF228.1.16 cells...

Ngày tải lên: 18/06/2014, 22:20

... concentration of viral pro- Figure of hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells ... and re-appearance of intracellular viral pro- Figure Confocal laser scanning image of intracellular RSV proteins Confocal laser scanning image of intracellular RSV proteins HEp-2 cells had been ... viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial cells HEp-2 cells had been infected at m.o.i of 50 for 12 h then incubated with anti-RSV...

Ngày tải lên: 20/06/2014, 01:20

... C: CXCR4 staining of noninduced Jurakt cells Panel E and G: CXCR4 staining of induced Jurkat cells The fluorescent cell in panel G is representative of a minor population of cells in the induced ... down-regulation of CXCR4 from the cell surface CXCR4 down-regulation may be due in part to intracellular sequestering of HIV glycoprotein/ CXCR4 complexes Methods Cells and virus Cells of the RH9 subclone of ... Immunofluorescence microscopy demonstrating reduced cell surface expression of CXCR4 in HIV-1 infected RH9 cells Immunofluorescence microscopy demonstrating reduced cell surface expression of CXCR4...

Ngày tải lên: 20/06/2014, 01:20

... IM28 on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) and ... fusion of 293/CD4+ cells with TF228.1.16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228.1.16 cells (data not shown) The fusion of TF228.1.16 cells ... dexamethasone (B) cells are mainly exploded vs in the presence of IM28 Figure dexamethasone on fusion of TF228.1.16 cells with SupT1 cells Effect of2 Effect of dexamethasone on fusion of TF228.1.16 cells...

Ngày tải lên: 20/06/2014, 04:20