1 insert data in cells and ranges

... but in concert with a number of conserved domains, including a GTPase domain Multidomain proteins that encode functional kinase domains often utilize intrinsic protein kinase activity distinct ... protein levels Data from transiently transfected HEK 293T cells indicate that LRRK2 and carboxyl terminus of heat-shock protein (HSP)-70-interacting protein (CHIP) interact via the ROC domain and ... substructures within the kinase domains, with the expectation that similar kinases may be involved in similar roles in cells LRRK1 and LRRK2 are awkwardly nestled within the tyrosine-kinase-like family,...

Ngày tải lên: 23/03/2014, 04:20

... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...

Ngày tải lên: 23/03/2014, 13:20

... in PBS containing 0.1% Tween-20 Mouse mAb 2E3 (Abnova) against rLRG1 was added at a 1:50 dilution in blocking buffer and incubated overnight at 4°C Cells were washed and incubated in a 1:50 dilution ... NIH:OVCAR5 cells may be indicative of cells in the process of secreting LRG1 Examination of ovarian tumor extracts and cell lines by Western blot revealed increased expression of LRG1 protein in malignant ... frozen in liquid nitrogen within 30 of resection and stored in the vapor phase of liquid nitrogen Tissue sections were made from each sample, stained with hematoxylin and eosin (H&E), and examined...

Ngày tải lên: 20/06/2014, 07:20

... Koutsouris, A S Andreou, C Pattichis, and C Schizas, “Multipurpose health care telemedicine system,” in Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology ... priority) TESTING AND RESULTS The described system was implemented to be used in two main test applications: telemedicine in emergency handling and remote health care for patients In this paper, ... chain, from health information and prevention all the way through to rehabilitation and long-term care A potential advantage of including telemedicine in this environment is to give a standard and...

Ngày tải lên: 22/06/2014, 19:20

... reduction in SDF-1 production in increased OCP mobilization in vivo needs to be further confirmed using a model where SDF-1 concentration in the bone marrow is maintained in the presence of TNF Since ... CKO and control mice were double stained with FITC-anti-CD11b and allophycocyanin (APC)–anti-CXCR4 antibodies, and CD11b+ cells were gated Intensity of CXCR4+ stained CD11b+ cells shown Black line, ... downregulates SDF-1 mRNA expression in osteoblasts [17] TNF did not increase G-CSF in ST2 cells (data not shown), however, suggesting that the reduction in SDF-1 induced by TNF in vitro is not mediated...

Ngày tải lên: 09/08/2014, 10:23

... longitudinal structure in the ionosphere - j0 calculated from both types of data showed that: in spring equinox the value of j0 is highest, smaller in autumn equinox and then in summer, in winter ... EEJ and inversely in the equinoxes - j0 calculated from station data at HUA is greater than that at BCL during equinoxes and have equal amplitude as in the summer But j0 calculated from CHAMP data ... the data points in the cell The data in each cell which have the deviation >2nT than the average value in the cell are removed After the such gridded process, the remaining number of data points...

Ngày tải lên: 10/09/2015, 14:05

... to the main analysis stage, e.g., data integration, cleansing, transformation and reduction Data integration aims at combining data residing in different sources and providing a unified data perspective ... perspective Data cleansing refers to determining inaccurate, incomplete, or unreasonable data and amending or removing (transforming) these data to improve data quality Data reduction aims at decreasing ... the data under processing and analysis for different application domains Following and depending on the data embedding, further data correlation/clustering and inference may be attained, in which...

Ngày tải lên: 06/06/2017, 15:43

... to the main analysis stage, e.g., data integration, cleansing, transformation and reduction Data integration aims at combining data residing in different sources and providing a unified data perspective ... perspective Data cleansing refers to determining inaccurate, incomplete, or unreasonable data and amending or removing (transforming) these data to improve data quality Data reduction aims at decreasing ... the data under processing and analysis for different application domains Following and depending on the data embedding, further data correlation/clustering and inference may be attained, in which...

Ngày tải lên: 21/06/2017, 15:51

... have a stronger incentive to reinvest in new and more efficient technologies instead of running the old plant in order to keep receiving tariff payment In Germany and Spain, in the 1990s, tariff ... support instruments occurred, including R&D spending and tax credit schemes up to net metering and, most recently, FITs In Singapore, a similar progression occurred from R&D spending to investment ... applied in order to foster the deployment of solar PV installations around the world, including research and development (R&D) spending, investment and tax incentives, and market-based support instruments...

Ngày tải lên: 30/12/2017, 12:54

... housing associations have little influence on house building In those countries, the process of developing and building houses is mainly a private initiative Integration of PV systems in buildings ... implement the housing construction process, in which the main opportunities are for PV roof integration in single-family terraced houses and for faỗade and roof integration in apartment buildings (Figure ... integrated into the roof) and integration of PV in building components (awnings, shading devices, etc.) (see examples in Figure 3) 1.33.4.1.1 Roof-integrated PV A PV system can be integrated into the...

Ngày tải lên: 30/12/2017, 13:05

... role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 ... cells incubated with the MAPK kinase (MEK) inhibitor, U0126 U0126 completely inhibited the cyclin D1 promoter activity in AA-pretreated SMC incubated with IL-1b and reduced it in cells incubated ... of BrdU into SMC was strongly stimulated by incubation in medium containing 10% (v/v) fetal bovine serum Although the incorporation of AA alone did not affect BrdU incorporation, incubating these...

Ngày tải lên: 19/02/2014, 16:20

... heat inactivated fetal bovine serum (FBS); 1% nonessential aminoacid, 1% sodium pyruvate and 1% antibiotic penicillin and streptomycin (all from Gibco, Scotland, UK) and were maintained under standard ... endocannabinoids may control brain innate immunity in MS by acting in different CNS cell types such as astrocytes and microglial besides immune cells [rev [56]] Activating or inhibiting the innate ... of staining intensity (Figure 3D) The increased anandamide tone limits microglial activation in mice infected with Theiler’s virus The intracranial injection of Theiler’s virus induced an increase...

Ngày tải lên: 19/06/2014, 22:20

... radio-sensitizer in hypoxic cells DCQ radiosensitization in the FHs74Int normal intestinal cell line After establishing effects of DCQ and IR in cancer cells, we compared DCQ efficacy in normal cells For ... fold in oxic cells and by 9.8 fold in hypoxic cells It is interesting to note that the significant inhibition of Cox-2 protein by DCQ in hypoxic and irradiated cells is associated with increased ... examined in the FHs74Int normal human intestinal cell line to determine the sensitivity of normal cells to DCQ In addition, the DNA damaging potential of DCQ and its effects on the protein expression...

Ngày tải lên: 09/08/2014, 10:21

... of injection, the hearts were harvested, washed in PBS, and fixed in 10% formalin overnight at 4°C Paraffin embedded tissues were cut into 5-μm sections and stained by Masson’s Trichrome staining ... in prolactin-mediated cell proliferation and angiogenesis in human endothelial cells Int J Mol Me 2002, 10:433-440 Zeng B, Ren X, Lin G, Zhu C, Chen H, Yin J, Jiang H, Yang B, Ding D: Paracrine ... protocols[9], and transplanted MSCs over-expressing HO-1 into acute myocardial infarction hearts Our data indicate that over-expression of HO-1 in MSCs enhance angiogenesis and improves heart function in...

Ngày tải lên: 10/08/2014, 05:21

... DCs and macrophages in cultures BM cells were isolated by flushing femurs and tibiae with PBS Pelleted cells were briefly resuspended in water to lyse red blood cells and stabilized by adding ... allowing DCs and macrophages to resist HSV-1 replication Materials and methods Virus, cells, and mice Triple plaque purified HSV-1 strains McKrae, KOS, and GFP-VP22 were grown in rabbit skin (RS) ... 2009, 6:56 tries [16-19] Both macrophages and DCs perform crucial roles in linking innate and adaptive immunity and augmenting the immune response to HSV-1 infection It was previously shown that...

Ngày tải lên: 12/08/2014, 04:21

... DCs and macrophages in cultures BM cells were isolated by flushing femurs and tibiae with PBS Pelleted cells were briefly resuspended in water to lyse red blood cells and stabilized by adding ... allowing DCs and macrophages to resist HSV-1 replication Materials and methods Virus, cells, and mice Triple plaque purified HSV-1 strains McKrae, KOS, and GFP-VP22 were grown in rabbit skin (RS) ... 2009, 6:56 tries [16-19] Both macrophages and DCs perform crucial roles in linking innate and adaptive immunity and augmenting the immune response to HSV-1 infection It was previously shown that...

Ngày tải lên: 12/08/2014, 04:21

... centrifuged for minutes at 1,000 g Murine IL-1β, IL-6, and TNFα levels were determined using the Luminex multianalyte technology and the BioPlex system in combination with BioPlex Mouse Cytokine Assays ... protection against cartilage destruction and a significant reduction in the amount of synovial inflammatory cell infiltrate and joint space inflammatory cell exudate (Figure 4c) TNFR1 silencing in the ... organs, such as liver and spleen, are rich in mature and functional cells of Page of 11 Figure Effects of silencing tumor necrosis factor receptor (TNFR1) in synovial lining cells during streptococcal...

Ngày tải lên: 12/08/2014, 12:20

... expression of the human p32 protein [23] In this study, we investigated the effect of human CyclinT1 and CRM1 expressed in rat T cells and macrophages, including primary cells, in order to identify a molecular ... and hCRM1 in spleen- and thymusderived cells (C), total T and CD4+CD8- T cells (D), and macrophages (E) in WT or Tg rats was confirmed by Western blotting using anti-hCycT1 and anti-hCRM1 T cells ... of infectivity Interestingly, R5 viruses produced in rat T cells, including the JR-CSF [27], YU-2 [28], and NL-AD8 [29] strains, were equally infectious compared to those produced by human T cells, ...

Ngày tải lên: 12/08/2014, 23:20

... min, rinsed with PBS and then incubated with Steptavidin-HRP (Dako) for 30 Immunostaining was revealed using 3,3’-Diaminobenzidine substrate solution according to the manufacturer’s guidelines ... virus to both CD3hi and CD3lo thymocytes, and may explain how thymocytes are infected with R5 virus pDC are located within the cortex and medulla in uninfected and HIV-1-infected thymus tissue ... are the 25 and 75 percentiles, the horizontal line in the box is the median and the whiskers extend to the minimum and maximum data points * indicates a p value of < 0.05 as determined by the...

Ngày tải lên: 13/08/2014, 01:20

... auto-activated protein kinase (AK), myelin basic protein kinase (MBPK), cytosolic protamine kinase (CPK), casein http://www.retrovirology.com/content/5/1/115 kinase II (CKII) and protein kinase C (PKC) ... H3B and Hut-78 cells were mixed and incubated at 37°C for 40 mins, cells were then lysed and virus protein immunoprecipitated using heat-inactivated AIDS patient antibody cross-linked to protein ... many proteins that interact with nucleic acids, including HIV proteins Tat, and Rev [32,33] and RNAPII [19,34] Indeed phosphorylation of HIV RT in vitro led to increased polymerase and RNase...

Ngày tải lên: 13/08/2014, 05:21