1 epidermal langerhans cells in irritant and allergic positive patch test reactions
Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
Ngày tải lên :
21/06/2014, 03:20
... and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing ... mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups with seven mice in each ... DNR-mediated apoptosis in HepG2r/ADM cells, we investigated apoptosis-related protein expression in the cells (Figure 6C) DNR or Cdte QDs cannot induce apoptosis strongly in HepG2r/ADM cells due to multidrug...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
Ngày tải lên :
09/08/2014, 01:21
... centrifugation Cells were resuspended in buffer containing 0.1% bovine serum albumin and 0.1% NaN3 at 106 cells/ ml Nonspecific antibody binding at the Fc receptor was blocked by treating 106 cells with ... antigen-experienced T cells, are mainly CD28– and can also express CCR5 [10,23,24] The fraction of CD28– T cells is also expanded in WG and is correlated with organ involvement [25–28] These findings support ... CCR5+ and CCR3+ T cells owing to enhanced recruitment into inflammatory sites Downregulation of the cell-surface expression of the inducible inflammatory chemokine receptors CCR5 and CCR3 during...
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Báo cáo y học: ": Prevalence of reverse transcriptase and protease mutations associated with antiretroviral drug resistance among drug-naïve HIV-1 infected pregnant women in Kagera and Kilimanjaro regions, Tanzania" pptx
Ngày tải lên :
10/08/2014, 05:21
... care and women attending postnatal care in Bukoba (Kagera) and Moshi (Kilimanjaro) municipalities were included in the study All women underwent pre- and post -test counselling and signed informed ... provide NVP-containing HAART to HIV-1 infected pregnant women who received sdNVP in Kilimanjaro and Kagera regions Information concerning drug status including exposure to sdNVP and/ or absence ... risk of virological failure in women initiating HAART after sdNVP is associated with the time interval between receiving sdNVP and initiating HAART [30] These results indicate that it is advantageous...
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báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx
Ngày tải lên :
11/08/2014, 00:22
... assay and GEM assay experiments, assisted in data analysis and interpretation and in writing and revising the manuscript SP and JG carried out the Western blot experiments CH and SA assisted in ... proangiogenic and pro-inflammatory factors, VEGF, COX-2, IL-6 and IL-8, in cultured human colon cancer cells; (c) DCPIP and DCPIP NPs are similarly effective in inhibiting VEGF-induced angiogenesis in ovo; ... the design planning of all experiments PP participated in the design of XTT, CAM and Western experiments, assisted in data analysis and interpretation and in writing and revising the manuscript...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
Ngày tải lên :
12/08/2014, 14:20
... Harvested cells contained mostly prominin-1 -positive cells (A, right) Further, prominin- 1positive cells were isolated using magnetic cell sorting and cultured in the presence of different cytokines and ... bleomycin-induced pulmonary fibrosis by prominin-1+ cells is stage-specific Expanded prominin-1 -positive cells were isolated using magnetic cell sorting and transplanted into recipient mice by intratracheal ... of prominin-1+ progenitors during acute pulmonary inflammation We injected intratracheally bleomycin and, after days, sorted prominin-1 + cells from the inflamed lung and expanded them in the...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
Ngày tải lên :
23/03/2014, 13:20
... F12 containing 10% fetal bovine serum, mM L-glutamine, and antibiotics (100 UÆmL)1 penicillin and 0.1 mgÆmL)1 streptomycin) [37] D407 cells were cultured in DMEM containing 10% fetal bovine serum, ... HO-1 in human RPE cell lines In contrast to interferon-c, hypoxia induced the expression levels of HO-1 mRNA in D407 cells and exerted no or only marginal effects in ARPE-19 cells (Fig 2) In D407 ... ARPE-19 cells, indicating that Bach1 is not a sole determinant for HO-1 mRNA expression in ARPE-19 cells Likewise, hypoxia induced expression of HO-1 and Bach1 mRNAs in D407 cells, indicating that...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
Ngày tải lên :
20/06/2014, 00:20
... examination[16] An[17] reported that the intradiscal administration of osteogenic protein-1 by injection in vivo resulted in an increased disc height present at 2, 4, and weeks and an increase in ... ovine intervertebral disc nucleus pulposus cells grown in alginate bead culture by Transforming Growth Factor-beta(1) and insulin like growth factor-I Cell Biol Int 2001, 25(7):679-89 Loveland ... Orthopaedic Surgery and Research 2006, 1:9 Background Intervertebral disc(IVD) degeneration and associated spinal disorders are a leading source of morbidity, resulting in substantial pain and increased...
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Báo cáo khoa học: " An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats" pdf
Ngày tải lên :
07/08/2014, 18:21
... lanoitubirtsid eht ,hguohtlA ]8,3[ sllec enircodneoruen lla fo ”srekram“ nommoc yltnacifingis a ,stneitap aitreni cinoloc nI slamina fo sutats evitsegid ot detaler ylraelc erew sllec RI-GC fo segnahc eht ... enircodne ni A ninargomorhc fo noitartsnomed lacimehcotsihonummI T aganawI ,N arahianaY ,F otaS ,H ikhO ,Y otomihsaH 01 892-782 ,25 ,9891 lotyC lotsiH hcrA saercnap enircodne eninac eht ni II ninargoterces ... yltnacifingis a ,noitidda nI ]5[ esaesid s’nhorC dna ]5[ sitiloc evitareclu ,]41[ romut ,]61[ noitapitsnoc cinorhc htiw stneitap fo mutcer eht ni desaercni osla yeht dna ]43[ asocum cinoloc ni...
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Báo cáo y học: " Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium" pdf
Ngày tải lên :
10/08/2014, 05:21
... of injection, the hearts were harvested, washed in PBS, and fixed in 10% formalin overnight at 4°C Paraffin embedded tissues were cut into 5-μm sections and stained by Masson’s Trichrome staining ... in prolactin-mediated cell proliferation and angiogenesis in human endothelial cells Int J Mol Me 2002, 10:433-440 Zeng B, Ren X, Lin G, Zhu C, Chen H, Yin J, Jiang H, Yang B, Ding D: Paracrine ... intraperitoneal injection of the HO-1 inhibitor zinc-protoporphyrin (ZnPP, Porphyrin Products, Logan, UT, USA) at a concentration of 50 μmol/kg/day, starting two days before and continuing until days...
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Báo cáo y học: "Evidence that Gag facilitates HIV-1 envelope association both in GPI-enriched plasma membrane and detergent resistant membranes and facilitates envelope incorporation onto virions in primary CD4+ T cells" doc
Ngày tải lên :
12/08/2014, 04:21
... (monosialotetrahexosylganglioside) and its relevance of envelope assembly onto budding virions in primary CD4+ T cells Precisely, we examined whether a point mutation (L30E) in matrix domain of Gag known for disrupting Env incorporation ... phytohemagglutinin (0.5 ug/ml) and interleukin-2 (10-20 U/ml) before infection with virus stocks Primary CD4+ T cells were infected with equal infectivity titers of VSV-G pseudotyped pNL4.3 WT and pNL4.3 ... membranes in primary CD4+ T cells Primary CD4+ T cells infected with VSV-G pseudotyped pNL4.3 and pNL4.3 (L30E) were lyzed and intracellular p55, gp41 and CD59 contents were measured in total...
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Báo cáo y học: " Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells" pdf
Ngày tải lên :
13/08/2014, 05:21
... auto-activated protein kinase (AK), myelin basic protein kinase (MBPK), cytosolic protamine kinase (CPK), casein http://www.retrovirology.com/content/5/1/115 kinase II (CKII) and protein kinase C (PKC) ... H3B and Hut-78 cells were mixed and incubated at 37°C for 40 mins, cells were then lysed and virus protein immunoprecipitated using heat-inactivated AIDS patient antibody cross-linked to protein ... many proteins that interact with nucleic acids, including HIV proteins Tat, and Rev [32,33] and RNAPII [19,34] Indeed phosphorylation of HIV RT in vitro led to increased polymerase and RNase...
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Báo cáo y học: "Apoptosis resistance in HIV-1 persistently-infected cells is independent of active viral replication and involves modulation of the apoptotic mitochondrial pathway" pot
Ngày tải lên :
13/08/2014, 06:20
... expression in uninfected or HIV persistently-infected cell lines MMP induction and Bcl-2 and Bax expression in uninfected or HIV persistently-infected cell lines H9 and H9/ HTLVIIIB (A) and Jurkat and ... Biosciences) Annexin-V+/PI- cells representing early apoptotic cells, and annexin-V+/PI+ mostly representing necrotic cells were determined APO-BrdU staining Late apoptotic cells were determined with ... persistently-infected H9/HTLVIIIBcells in comparison with non-infected H9 cells Apoptosis resistance in HIV persistently-infected H9/HTLVIIIBcells in comparison with non-infected H9 cells A) H9 and H9/HTLVIIIB...
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Báo cáo y học: "Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells" ppt
Ngày tải lên :
13/08/2014, 09:21
... amount and we calculated the ratios of DNA amount in cells/ RNA template level in infecting particles (Fig 3) These results show that FL and FSpl RNAs are both packaged in infectious HIV particles and ... Apparent RTion efficiencies of FL and FSpl RNAs in HIV-1 infected cells induced a strong decrease (185-fold) of the FL cDNA amounts in infected cells In contrast, AZT induced only a 3-fold decrease ... FL and FSpl RNAs is achieved equally in intact virions 3.5 (DNA in cell/RNA in virion)x100 2.3 2.5 2 1.5 0.5 FL FSpl Figure cells infected Apparent RTion efficiencies of FL and FSpl RNAs in HIV-1...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
Ngày tải lên :
14/08/2014, 19:22
... effective in preventing allergen-induced airway inflammation in a murine allergic model OVA-sensitization and challenge could induced the development of airway inflammation and Th2 responses and suppressed ... vaccine containing OVA and Fc fusion gene targeting DCs, the murine OVAcDNA was amplified from OVA-pcDNA3.1 plasmid by polymerase chain reaction (PCR), spliced and then cloned into pMIgV containing ... both activating and inhibitory FcRs, and the balance between activating and inhibitory signaling will determine whether uptake of immune complexes results in naive T cell activation and protective...
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Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy
Ngày tải lên :
10/09/2015, 09:25
... (erlotinib, efitinib, and Gifitinib, for example), target to the ATP-binding pockets within the EGFR tyrosine kinase (TK) domain and block the phosphorylation and the following signalling 3) ... such as binding with ligands and subsequent activation Interestingly, again, opposing effects were observed in different settings of experiments In artificial 20 membranes, the binding of EGF-EGFR ... for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments...
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Enhancing recombinant protein yield and quality using novel CHO GT cells in high density fed batch cultures 1
Ngày tải lên :
15/09/2015, 17:10
... online feeding strategy for CHO cells producing recombinant human interferon gamma (IFN-γ) and determining the impact on IFN-γ production and glycosylation quality, (2) Examining the signaling ... containing two to four cysteine-rich pseudo-repeats, a single transmembrane region and a conserved intracellular protein-protein interaction domains called death domain (Curtin and Cotter, 2003) Upon ... 107 cells/ mL in culture media containing 10% DMSO (Sigma, St Louis, MO) The vials were transferred into a cryogenic freezing container (Nalgene) and placed in a –70OC freezer The freezing container...
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Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"
Ngày tải lên :
25/10/2012, 10:51
... extracellular water, intracellular http://www.medsci.org Int J Med Sci 2011, water and body mass index were determined for each participant before dieting intervention and each six days following intervention ... decrease in their body mass index in an average of 8.1%±2.0% Changes in total body water had inverse correlation with changes in fat mass (r=0.86) and positive correlation with an increase in fat ... circulating cells as “circulating EPCs” was based on the work [28], in which blood–derived cells from which endothelial cells in culture were developed were described as cells expressing CD34...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
Ngày tải lên :
31/10/2012, 15:28
... ethanol-induced expression and activation of cytokines and chemokines in monocytes and macrophages (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the upper gastrointestinal ... Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived ... G-Protein-MAPK, NGF, and PDGF signaling This finding corroborates the interaction observed between various intracellular signaling pathways and apoptosis induced by 1mM concentration of ethanol in...
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PRIVATE ENTREPRENEURS IN CHINA AND VIETNAM PART 1
Ngày tải lên :
07/11/2013, 21:15
... stronger in China than in Vietnam Also the proportion of larger, state-owned companies is in the fields of industry, commerce and banks higher in China than in Vietnam As a result in China the ... self-reliance, since the beginning of the reforms has had a magnetic effect on foreign investors China had far and away in the 1980s and 1990s the highest foreign investment of all developing nations In that ... of earning more money and mounting insecurity concerning future developments in the public sector, a continually increasing number of trained employees from the state sector are becoming self-employed...
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Tài liệu PRIVATE ENTREPRENEURS IN CHINA AND VIETNAM PART 2-1 ppt
Ngày tải lên :
15/12/2013, 06:15
... consisted of making the appointment and a short introduction In each case we carried out a qualitative interview lasting about two hours using guidelines, then followed questions using a standardized ... whom 169 were men and women; 69 in Zheijang, 60 in Henan and 49 in Gansu of whom 108 were in urban areas and 70 in rural 1.1.2 The survey in Vietnam In Vietnam we concentrated in the phase of the ... capital from ethnic Chinese in In 1997 Ha Bac was divided into two new provinces Bac Ninh and Bac Giang Interview with the director of the Office for Industry in Ho Chi Minh City 26 November 1996...
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