... 57 and CGI-55 by PRMT1 The interaction of Ki-1 ⁄ 57 with PRMT1 and the presence and conservation of the RGG ⁄ RXR box motifs in the amino acid sequences of Ki-1 ⁄ 57 and CGI-55 suggest that these ... on the distribution of the two proteins between the nucleus and cytoplasm Future studies must address the detailed regulation of the cellular localization of these two proteins and if and how these ... influence its distribution between the nuclear and cytoplasmic compartments Treatment with Act D inhibits the synthesis and consequently the export of mRNA from the nucleus and we observed that it causes...
Ngày tải lên: 16/03/2014, 13:20
... because of the central role that ppUL44 plays in the synthesis of viral DNA [9,20,21] These data taken together with the observed defects in the onset and expansion of viral DNA synthesis suggest ... Recombinant viruses The top line represents the CMV genome with the region surrounding UL114 expanded below The second line represents the structure of the region in the parent virus (AD169) The third ... DNA and the data were normalized relative to the input copy number (Fig 8) During the first 18 h of infection, copy number of the wt and deletion virus genomes decreased at the same rate with...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA" potx
... because of the central role that ppUL44 plays in the synthesis of viral DNA [9,20,21] These data taken together with the observed defects in the onset and expansion of viral DNA synthesis suggest ... Recombinant viruses The top line represents the CMV genome with the region surrounding UL114 expanded below The second line represents the structure of the region in the parent virus (AD169) The third ... DNA and the data were normalized relative to the input copy number (Fig 8) During the first 18 h of infection, copy number of the wt and deletion virus genomes decreased at the same rate with...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo y học: "α The role of HIF-1α in maintaining cartilage homeostasis and during the pathogenesis of osteoarthritis" pot
... growth plate chondrocytes [11] Conclusion The results provided in the study by Yudoh and colleagues and previous reports by us and other groups strongly support the notion that HIF-1 is of pivotal ... Using RNA-interference, Yudoh and colleagues [1] further analyzed the role of HIF-1 in chondrocytic energy generation, matrix synthesis and cell survival During hypoxic and normoxic conditions, functional ... glucose uptake and utilization in order to compensate for the accelerated energy consumption during OA In conclusion, the study by Yudoh and colleagues, in concert with previous reports, further characterized...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo y học: " EBNA3C interacts with Gadd34 and counteracts the unfolded protein response" docx
... weakly with SUMO-1 and fail to interact with SUMO-3[1] To test whether interaction between EBNA3C and Gadd34 also depends on the SUMO interaction motif, the m2 mutation was constructed in the context ... http://www.virologyj.com/content/6/1/231 well as with RBP-J-Kappa, and LacZ conversion occurred within 30 minutes for both interactions These data indicate that EBNA3C interacts with Gadd34aa395-674, and that the interaction ... in the ER, Bip dissociates from ATF6, and ATF6 translocates to the golgi where it is cleaved by site and site proteases, resulting in the disappearance of the full length 90 KDa protein, and the...
Ngày tải lên: 12/08/2014, 04:21
Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx
... nondenaturing PAGE Positions of the top and middle bands coincided well with the two bands observed with the purified enzyme Thus, it was suggested that the middle and top minor bands of the Ôcomponent ... staining, and only the upper one stained upon activity staining (data not shown) The Mr values for the upper and lower bands were 220 000 and 200 000, respectively These data suggest that the subunit ... performed, and the monitor and the packing values were checked to determine the result from the candidates We concluded that there is an (abc)2 dimer in an asymmetric unit of the cell The ˚ calculated...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... pGBT9NBR1333 and pGBT9NBR1Cterm were able to interact with full-length NBR1 (see Fig 1B, i,iii,iv and vi) FEZ1 and CIB interact with each other To con®rm the interactions between NBR1, CIB and FEZ1, the ... pattern to the ER compartment was observed with the presenilin protein PS2 [20] and to the cytoplasm when coexpressed with Snk [19] Mutations in the presenilin genes PS1 and PS2 cause the majority ... fragments and further tested by the yeast two-hybrid method (see Fig 1B, v±viii) Both CIB and FEZ1 interact strongly within the same region of NBR1, encoding amino acids 451±597 and weakly with amino...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... Þ=n where A and B are the MICs of drug A and drug B in the combination, MICA and MICB are the MICs of drug A and drug B alone, FICA and FICB are the FICs of drug A and drug B and n is the number ... 40, and FITC-D 70), and then incubated with the peptide The data shown in Fig reveal that Esc(1–18) is able to cause the release of the 5654 four dextrans used in a dose-dependent manner, and with ... damage and loss of cellular integrity, with a partial discharge of the cellular contents, within 20 (Fig 11) These results correlate with the killing kinetics of the peptide, and show that the antibacterial...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo y học: "κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocyte" ppt
... dithiothreitol and 10 U/µl Superscript II™ reverse transcriptase in the first strand The reaction was incubated for 50 at 42°C and for 15 at 70°C The single-strand cDNA was then diluted and samples ... stimulated with cytokines were cultured and collected 72 hours after cytokine addition Concentrations of OPG were measured with a sandwich ELISA using the set of specific antibodies and the OPG/Fc standard ... expression and function of AP-1, NF-κB and Egr-1 in human RA synoviocytes by RT-PCR and by immunocytochemistry after induction with IL-1, TNF-α and IL-17 alone and in combination To assess the functional...
Ngày tải lên: 09/08/2014, 01:23
Báo cáo khoa hoc:" Simulation analysis to test the influence of model adequacy and data structure on the estimation of genetic parameters for traits with direct and maternal effects" ppsx
... By and large, a part of the males and females were randomly mated within-flock (within-flock mating), the other part being randomly mated with reproducers from other flocks (between-flock mating) The ... example, at the end of the simulation, the maternal grand-dams had still passed on 78% of the gene of their flock and the dams, 39% Therefore, six years were not sufficient to take into account the overall ... despite the theoretical advantages of this model, some data and model conditions can affect the validity and precision of the estimation of variance components The first source of bias lies in the...
Ngày tải lên: 09/08/2014, 18:21
Báo cáo y học: "Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells" ppt
... of the viral cDNA species from the earliest steps of RTion, i.e the minus strong-stop DNA synthesis (ssDNA) and the first strand transfer (represented by the U3 product) (Fig 1) Up to now, there ... contained FL RNA Since the amounts detected with either the ssDNA or the U3 primers were close to 2-times of those measured with the FL primers, they mostly correspond to the entire FL cDNA However, ... work with ZM help, and helped in drafting and finalizing the manuscript MM wrote the manuscript All authors read and approved the final manuscript Acknowledgements The authors thank M Biart for the...
Ngày tải lên: 13/08/2014, 09:21
Báo cáo y học: " Evolution of the HIV-1 envelope glycoproteins with a disulfide bond between gp120 and gp41" pdf
... the Q593L reversion occurred after the loss of the cysteine at position 605 (cultures L and Q, compare sequences from weeks and 10) The idea that the C605Y change has to precede Page of 11 (page ... not shown) We therefore studied the effect of the L593Q and Q591L substitutions in the context of the wt virus MT-2 T cells were transfected with the appropriate molecular clones and virus spread ... X) and the K487N substitution (in culture X4) were not tested further and are omitted from the scheme It is possible that these reversions contributed to the gain of replication capacity by the...
Ngày tải lên: 13/08/2014, 13:20
Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 1
... component of innate immunity, and are either inducible or constitutive4 They are present on the mucosal surfaces, on the body surface and within the granules of phagocytes within several minutes to ... upon interaction with micelles The region which is rich in tryptophan in the middle of the peptides interacts with one layer of the membrane and the two termini are oriented toward the aqueous environment70-72 ... is also related to the L- or D- enantiomer However the effect of enantiomer of the amino acid is dependent on whether the replacement affects the structure of the peptide and the amphipathicity...
Ngày tải lên: 14/09/2015, 21:54
KNOWLEDGEBASED INTERPRETATION OF REMOTE SENSING DATA WITH THE INTERIMAGE SYSTEM: MAJOR CHARACTERISTICS AND RECENT DEVELOPMENTS
... segments from the intersection of the regions associated to higher level hypotheses (from the father, semantic network node) and a shapefile provided by the user The output of the interpretation ... logical And or Or operations The Expression element enables the user to create named variables form the object hypotheses properties The values of those variables, are stored within the object hypotheses, ... for the Vegetation node in the semantic network shown in fig.1 Basically what the rule does is to select all the hypotheses generated in the top-down interpretation step, for the nodes Trees and...
Ngày tải lên: 01/10/2015, 08:46
The essence of object oriented programming with java and UML
... know what the buttons do, which ones you need to press to get the object to what you need, and what the lights mean about the status of the object The details of how the box is put together inside ... system, and are sent to the appropriate program objects, which then have the responsibility to respond with some action The order and timing of these messages is determined by the actions of the ... Chapter The UML originated in the mid-1990's from the efforts of James Rumbaugh, Ivar Jacobson, and Grady Booch (The Three Amigos) There is a standard specification of the UML coordinated by the...
Ngày tải lên: 22/08/2013, 14:52
Data Models, Datasets, and the ADO.NET Interface
... need the help of the TableAdapter here, because we’ll write our own ADO.NET interface code to connect to the database and get the data All we want from the wizard is the dataset with the data ... of data table inside the dataset That’s it! That is all you need to to get your typed dataset ready for use With the dataset taken care of, now comes the job of gathering the data from a data ... will use the data from the Itinerary table inside the RealWorld database You don’t have to worry about how data gets inside the table Our goal here is to get the data out and publish it with RS...
Ngày tải lên: 05/10/2013, 08:48
FOCUS ON - phrasal verbs with get, 1
... school There is a difference, however, between the passive formed with be and the passive formed with get: When the passive is formed with get, there is often (but not always) a suggestion that the ... With all the bad weather we've been having, the construction project has gotten way behind schedule get by get by & gets by getting by got by gottenlgot by 1 .get by p.v When you get by or get by ... this information gets out There was a huge scandal after the news got out get out (of) p.v When you leave your house and things that are relaxing and fun, you get out or get out of the house You...
Ngày tải lên: 01/11/2013, 12:20
Tài liệu Module 1: Introduction to Exchange 2000 and the Web Storage System pdf
... Microsoft Exchange, and then click Active Directory Users and Computers Expand the domain node, and then select the Users directory Right-click the Users directory point to New and then select User ... renamed and a new log file is started If a database fails, you can recover the transactions by restoring the data from the log files All of the databases in a store share the same transaction logs and ... Public Folders In the MBX folder, expand the Administrator folder Switch to Outlook Expand Public Folders, and then expand All Public Folders Note the similarities between the Outlook and Windows Explorer...
Ngày tải lên: 11/12/2013, 14:15
Tài liệu Embedding Perl in HTML with Mason Chapter 6: The Lexer, Compiler, Resolver, and Interpreter Objects doc
... instances of another class, as the Interpreter does with Request objects, it will accept the created class's parameters in the same way, passing them to the created class at the appropriate time ... settings are the Compiler's allow_globals parameter/method and the Interpreter's set_global() method These let you declare and assign to variables in the package you specified with in_package, without ... method, and they will find their way there Altering Every Component's Content Several access points let you step in to the compilation process and alter the text of each component as it gets processed...
Ngày tải lên: 14/12/2013, 12:15