1 create a backup copy of the primary database datafiles

... GCTCAGTGGTGGA-3 and 5-CTGAGGGAAGCAAG AATGGA-3, OXI1 (At3g25250): 5-GACGAGATTATC AGATTTTACGC-3 and 5-AACTGGTGAAGCGGAAG AGAC-3, PTI1-4 (At2g47060): 5-CCCCAAAGAAAATG AGTTGCT-3 and 5-GCATCATTTCCTGGAGGAAAG-3 ... to assess the kinase activity and GST alone was used as a negative control The top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the Coomassie Brillian ... and the genomic sequence of OXI1 with the 5¢UTR and 3¢UTR was amplified by PCR and cloned in the pCambia 3300 The HA tag was cloned at the SalI site found at the ATG site of OXI1 Plant material...

Ngày tải lên: 14/02/2014, 19:20

... The N-terminal pro domain is autocatalytically cleaved off when the enzyme has obtained its active conformation, and the two C-terminal domains are cleaved off by heat treatment at 50 °C An Ala-Pro-Thr ... SPRK also has an aspartic acid residue at position at 200, and with the same conformation as in the two other structures, but Asp200 forms a salt bridge to Lys253 (Ala253 and Asn250 in PRK and ... from a Serratia sp R Helland et al Structure in the binding region The electrostatic surface calculated by icm (Fig [28]) shows that the bacterial peptidases have a more anionic character than the...

Ngày tải lên: 19/02/2014, 07:20

... recognition and cleavages of natural substrates, and the intrinsic stability of the enzyme [7,9] Furthermore, it has also been reported that the CT-peptide may act as a partial inhibitor of PC1 ⁄ in the ... located at the N- and the C-termini can negatively or positively cooperate in the activation of an enzyme In the case of proPC1 ⁄ 3, removal of the various structural and functional domains is a ... was characterized by western blotting, amino acid analysis and N-terminal Edman sequencing (data not shown) MS analysis showed that the isolated CT-peptide had a molecular mass within Da of the...

Ngày tải lên: 30/03/2014, 08:20

... variants is a characteristic feature of RNA viruses RNA dependent RNA polymerase and reverse transcriptase are error-prone Table 12: The frequency of variant amino acids in Helical Domain II of ... enzymes and have been implicated as a cause for the generation of variants [76,77] The mutational changes in the protease and reverse transcriptase, depending on their location, may impact on their ... targeting these enzymes The viruses containing alterations may then be able to evade the inhibitory activities of the agents and are designated as drug-resistant variants Similarly, the mutations...

Ngày tải lên: 20/06/2014, 01:20

... The A to Z of Australian and New Zealand Cinema, by Albert Moran and Errol Vieth, 2009 49 The A to Z of of African-American Television, by Kathleen FearnBanks, 2009 50 The A to Z of of American ... Anglicanism, by Colin Buchanan, 2009 60 The A to Z of Scandinavian Literature and Theater, by Jan Sjåvik, 2009 61 The A to Z of the Peoples of the Southeast Asian Massif, by Jean Michaud, 2009 62 The ... Discovery and Exploration of Australia, by Alan Day, 2009 28 The A to Z of the United Nations, by Jacques Fomerand, 2009 29 The A to Z of the “Dirty Wars,” by David Kohut, Olga Vilella, and Beatrice...

Ngày tải lên: 02/07/2014, 04:21

... recommendations, and a scientific/technical part The scientific/technical part is essential to the Position Paper as it contains all facts and arguments that form the basis of the EPS position (i) The ... operational in 2030 Accelerator Driven Systems (ADS) offer the possibility of the transmutation of plutonium and the minor actinides that pose the main long-term radioactive hazard of today’s fission ... of the attitude towards nuclear energy taken by the institution that carried out the analysis Climate change Since the beginning of industrialisation the world has experienced a rise in average...

Ngày tải lên: 08/08/2014, 15:21

... situations, such as that reported in Figs and 5, the importance of maintaining the local level of IL-1Ra became dramatically apparent, as were the advantages of gene transfer as a means of protein ... this material Gene delivery may offer the greatest chance of early success Recent data from our laboratory have shown that the synovial lining is capable of maintaining therapeutic levels of transgene ... cellular level, the advantage of gene transfer as a means of drug delivery arises from the sustained availability of IL-1Ra that this method permits Materials and method Materials Ham’s F12 medium,...

Ngày tải lên: 09/08/2014, 01:23

... skeletal extent of PDB is on the basis of X-ray evaluations bone biopsy showed the presence of an osteogenic sarcoma on the Pagetic bone already metastasized to the lungs The patient died at 65 years ... by an autoanalyzer, has been performed also in all the individuals undergoing mutational analysis The upper limit of the reference range is 120 units/l DNA extraction, PCR and mutational analysis ... analysis AT supervised the performance statistical analysis AA helped in the clinical activity AC participated in the sequence alignment GI participated in the design of the study and helped to draft...

Ngày tải lên: 09/08/2014, 07:20

... leukemia Carcinogenesis 2005, 26:1382-1388 Watanabe M, Ohsugi T, Shoda M, Ishida T, Aizawa S, Maruyama-Nagai M, Utsunomiya A, Koga S, Yamada Y, Kamihira S, Okayama A, Kikuchi H, Uozumi K, Yamaguchi ... (RelA) and RelB [19] There are multiple pathways to activate NF-κB The two most common pathways are the canonical and the non-canonical pathways [20,21] In the canonical pathway, proceeding the ... been established as a cellular target of Tax and an essential component in Tax-mediated NF-κB signaling in both canonical and non-canonical pathways Therefore, we reasoned that the specific targeting...

Ngày tải lên: 10/08/2014, 05:21

... courses, an average of five courses were planned with a mean budget of £9,000 They have an average of three active tutors, and Leads dedicate an average of 21% of their working week to the Programme ... collection, analysis and interpretation, and drafted the manuscript AK contributed to the design, analysis and interpretation of data, and revised the manuscript AR contributed to the design, analysis and ... interpretation of data, and revised the manuscript All authors read and approved the final manuscript Acknowledgements Ms Caroline Gardner Dr David Reeves The Process Evaluation of the EPP (PREPP) at...

Ngày tải lên: 11/08/2014, 05:22

... conception and design; CF acquisition of data; MCS statistical analysis and interpretation of the data; MCS and CF drafting the manuscript; AB, MF and WR interpretation of data, critical revisions of the ... false-negatives, at self-report, seven of them overlapping with the false-negatives at FCSE Most of those 25 falsenegatives used a medical disease they actually had as a kind of umbrella for other ... longitudinal assessment of the case and all data from medical records were evaluated to form a diagnosis of Clinical Somatizers (CS), taking into account the number of validated unexplained symptoms...

Ngày tải lên: 11/08/2014, 16:22

... Institutes of Health research associateship This research was funded in part by the Intramural Program of the NIH, NIAID, including the NIH Intramural AIDS Targeted Antiviral Program References ... (0.5% final) and quantified by spectrophotometric measurement of β-galactosidase activity as described previously [36] For each data point, error bars indicate the standard errors of the mean of duplicate ... fusion assay Env-mediated cell fusion activity was measured using a quantitative vaccinia-based reporter gene assay as described previously [36,37] Each vaccinia virus was used at a multiplicity of...

Ngày tải lên: 12/08/2014, 23:22

... 17 cases of primary ES involving the vagina and/or vulva in the literature[6] A few cases of primary neuro ectodermal tumors (PNET) in the pelvis have also been reported [7] Unusual metastasic ... percent of ES at diagnosis have radiographic evidence of metastasis Lungs and bone are the main metastatic locations Radiologically, an aggressive osteolytic lesion is commonly observed ES is characterized ... sarcoma Clin Imaging 1997, 21(1):23-6 doi:10.1186/2045-3329-1-9 Cite this article as: Vanel et al.: Vaginal metastasis of a Ewing sarcoma five years after resection of the primary tumor Clinical Sarcoma...

Ngày tải lên: 13/08/2014, 15:21

... video, animation and audio – based on different themes each year Besides promoting teamwork and collaboration, it also aims to enhance the development of media literacy among students and teachers ... literacy and numeracy skills Information and Communication Technology (ICT) offers additional ways to learn and demonstrate learning It allows teachers and pupils to: • manipulate images and data; ... the area of creative digital media, and to encourage user-created teaching and learning resources Students and teachers are encouraged to express themselves creatively through different media...

Ngày tải lên: 12/10/2015, 17:35

... caspase-6 and caspase-7) are effectors of apoptosis Caspase-2, caspase-8, caspase-9 and caspase-10, function upstream of the effector caspases in apoptosis and are known as initiator caspases A phylogenetically ... RNA Samples were regarded as pure if A2 60/280 was approximately The ratio of the absorbance at 260 and 230 nm (A2 60/230) was used as a secondary measure of RNA purity from organic compounds Samples ... research and for helping me with the intra-tracheal influenza infection Dr Maria Papathanasopoulos (University of the Witwatersrand, South Africa), Associate Professor Tham Foong Yee (National...

Ngày tải lên: 13/10/2015, 16:41

... examining the costing plan for a two-layer jacket (Appendix 5, Table 2) After calculating manufacturing cost, 10% of profit rate is added then 10% of VAT (value-added tax) and another 10% retail ... sustain this advantage to maintain high profit rate over a number of years This definition uses profit rate as a measurement to rate the competitive advantage In other view, we can say that a product ... production capacity and company address Company participates frequently in trade fairs, which are organized frequently over the course of a year, especially in fashion fair and new year fair Thaloga also...

Ngày tải lên: 23/04/2013, 10:29

... on vacation indicating low population hence the variation in the monthly sludge generation The estimation of the biogas potential of the sludge the quality of the sludge was analysed and the ... 2007 [7] Barelli D., Csambalik L., Mestas C and Santos D Economical and Environmental Analysis of a Biogas Plant within the Context of a Real Farm The Royal Veterinary and Agricultural University, ... Figure Layout of KNUST sewage treatment plant Methodology Sample of the sewage from the PST was collected and analysed to determine the quality of the sewage The main component of interest was the...

Ngày tải lên: 05/09/2013, 16:11

... to create the array, specifying the type and the number of elements in the constructor argument instead of using square brackets in the declaration The managed array is a reference type, so the ... demonstrated the declaration and use of managed aggregate types, including ref classes, value classes, managed arrays, enum classes, and interface classes In the next section, you’ll learn about features ... seed) { array^ atoms = gcnew array(num_atoms); // Initialize the array // We cannot use a for each statement here because the for each // statement is not allowed...

Ngày tải lên: 05/10/2013, 08:20

... on a laptop and the X application (i.e., client) that you want to run is located on a remote system You can arrange to have the application output display on the laptop The following paragraphs ... instead of as it was earlier export DISPLAY=casper:14 Since the ssh server can have many tunneled X sessions attached at the same time, the display number increases with the number of sessions attached ... Machine C Now that authorization for Machine C to connect to the display on Machine A is in place, the last task is to set the DISPLAY variable for the X client on CHAPTER 21 ■ A BRIEF TOUR OF...

Ngày tải lên: 05/10/2013, 08:51