0001 growth and structural characterization

... better Ramachandran plots were chosen to represent the three-dimensional solution structure of conomarphin, and the mean structure was generated by molmol Structural characterization and comparison ... for structural studies of conotoxins and their analogs [24–27] Most NOESY crosspeaks were assigned and integrated, with concomitant cycles of structure calculations for evaluation of distance and ... kinetics of the Trp14 and Thr15 amide protons The two observed small 3JHN-Ha coupling constants for residues Asn11 and Ser12, and the dNN(i,i + 2), daN(i,i + 2) [Phe13(CaH)–Thr15(NH)] and daN(i,i + 3)...

Ngày tải lên: 18/02/2014, 17:20

... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... HPA II patients and seven HPA III patients 2050 Characterization and functional analysis of novel mutations Among the mutations identied in our HPA population, two (i.e p.Q301P and c.707-2delA) ... by the local ethics committee and performed according to the standards set by the Declaration of Helsinki The experiments were undertaken with the understanding and written consent of all subjects...

Ngày tải lên: 16/03/2014, 01:20

... the sample Fig 3(d) and e show the crystallites in the samples with x ¼ 0:21 and 0.31 For these cases the particles are small (generally between 20 and 30 nm), defective and without a definite ... refined parameters are shown in Table and the experimental XRD and calculated profiles are displayed in Fig 5(a) and (d) It is reasonable that the substitutional and interstitial sites have equal ... Figs 11 and 12 show the particle morphology and stoichiometry determined by SEM and EDX examinations of the hematite-tin oxide system for x ¼ ¨ 0:4: Our XRD, Mossbauer spectroscopy, TEM and EDX...

Ngày tải lên: 19/03/2014, 16:48

... a strict substrate specificity towards inosine and guanosine This paper describes the cloning, recombinant expression and structural and functional characterization of purine nucleoside phosphorylase ... and the elution was carried out with : 95 (v ⁄ v) mixture of 95% methanol and 0.1% trifluoroacetic acid in H2O The retention times of inosine and hypoxantine, guanosine and guanine were 10.5 and ... thermophilus (TtPNP) [30], and E coli uridine phosphorylase [31] Substrate specificity and comparative kinetic characterization To elucidate the physiological role of PfPNP and its functional relationships...

Ngày tải lên: 23/03/2014, 09:21

... specifically and in significantly higher yields The glutathione protecting group was removed Fig MS characterization and HPLC separation of refolded states of PrP–S231C (A) Electrospray MS and deconvoluted ... from different secondary structural components The amide I band of PrP-WT in solution is centered around 1645 cm)1 due to the contribution from both random coil (30%) and a-helical structure (32%) ... GPIm) and some random coil (30% in PrP-WT, 23% in PrP–GPIm) After insertion of PrP–GPIm into membranes, ATR FTIR spectra were acquired for POPC and raft membranes containing PrP–GPIm at pH and...

Ngày tải lên: 23/03/2014, 10:21

... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... blue-native (BN) ⁄ PAGE and (2) colorlessnative (CN) ⁄ PAGE and BN ⁄ PAGE, we separated the Ndh complex from both MS and BS chloroplasts and determined its monomeric and dimeric state We also ... to the structural and functional characterization of this large complex in chloroplasts, we produced antibodies against Ndh subunits from the membrane (NdhE) and connecting (NdhH, -J and -K)...

Ngày tải lên: 23/03/2014, 13:20

... In this study we describe the identification and structural analysis of a Sial-lNnT unit in H influenzae LPS MATERIALS AND METHODS Bacterial strains and culture conditions The H influenzae strain ... v/v) and when appropriate, Neu5Ac (25 lgÆmL)1), CMP-Neu5Ac (50 lgÆmL)1), kanamycin (10 lgÆmL)1) or tetracycline (4 lgÆmL)1) Structural analysis and purification of LPS LPS was prepared for structural ... specifically cleaves sialic acid residues (Fig 1; lanes and 2) H influenzae LPS typically migrates as a complex series of bands in SDS/PAGE, each band corresponding to gain or loss of a glycose moiety...

Ngày tải lên: 23/03/2014, 21:21

... electrical and structural properties of AlGaN/GaN heterostructures The introduction of defects and/ or negative charges by the CHF3 into the GaN (or AlGaN/GaN heterostructure) was deduced by TEM and ... The CHF3/Ar gas flux was 20 sscm and the operating pressure in the chamber was × 10-2 mbar The control bias, the power, and the process duration were 200 V, 250 W and 300 s, respectively Afterwards, ... properties of the 2DEG, both macroscopic and nanoscale electrostructural analysis of the near-surface region of the samples were performed First, current-voltage (I-V) and capacitance-voltage (C-V) measurements...

Ngày tải lên: 21/06/2014, 05:20

... To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship has been such a help during this time Lastly, to my sweetheart Kriti Acharya, your love and support provided ... chemotherapeutic agent and in the mobilization and conditioning regimens for blood and marrow transplantation Reviewing the chemistry and pharmacology of cyclophosphamide is crucial for understanding its ... mustard and its mechanism of DNA cross linking Figure represents how phosphoramide mustard forms intrastrand and interstrand cross–links between guanine bases from DNA 23 B Hypothesis and approaches...

Ngày tải lên: 24/08/2014, 09:51

... background, characteristics and types of ferroelectric materials and previous works on relaxor PZN-PT single crystals Crystal growth, property and structural characterization and domain structures ... high-power-density and high-sensitivity sensors and actuators, etc In addition to the conventional flux growth technique, both the solution Bridgman growth technique [38,39] and the top-seeded solution growth ... Crystal Growth & Properties of PZN-PT Single Crystals Structural Characterization of PZN-PT Single Crystals 21 Domain Structures in Relaxor PZN-PT Single Crystals 28 Chapter 3: Growth and Longitudinal...

Ngày tải lên: 14/09/2015, 12:20

... insertion of $ 230 residues (residues 133–367) between strands D and E and a smaller insertion of $ 50 residues (residues 404–458) between strands F and G The hypervariable loop, FEBS Journal 273 (2006) ... expression, the SF21 and Hi5 cell lines and the Bac-to-Bac expression system and vectors were from Invitrogen (Carlsbad, CA, USA) Protease inhibitors were from Roche (Basel, Switzerland) and Ni-NTA resin ... penton base (yellow), hAd2 ⁄ 12 (blue), and hAd2 fiber peptide complex (pink) The fiber peptide is drawn as ball -and- stick and colored by atom fiber peptide bound and unbound structures reveal a high...

Ngày tải lên: 19/02/2014, 06:20

... analyze the biochemical and structural properties of CR I– II and to compare them with Calb I–II Our data show that CR I –II and Calb I –II share virtually the same EF-hand structures However, ... EF-hands of CR I–II compared to Calb I–II In contrast to Calb I –II, CR I–II shows no tendency to dimerize and both EF-hands of CR I–II bind calcium We conclude that the significant structural and ... underlined An EF-hand consensus sequence is placed between the aligned EF-hand I and EF-hand II sequences [3] The helices are shown as boxes with conserved hydrophobic (h) and other amino acids...

Ngày tải lên: 22/02/2014, 07:20

... surfaces was 1.4 A and a standard ˚ Stern layer was used to exclude ions from the 2.0 A molecular surface [37] The inner and outer dielectric constants applied to the protein and the solvent were, ... Concentrations of glucose, maltose and cyclohexaamylose were 80 mM , 80 mM and 1.25 mM , respectively The substrate concentration ranged between 0.025 and 0.5% (w/v) Fig SDS/PAGE and isoelectric focusing ... mass of 56 073 Da and an isoelectric point of 4.81 A search in GenBank revealed 86% and 67% sequence identity between the C sepium b-amylase and the b-amylase from sweet potato and soybean, respectively...

Ngày tải lên: 22/02/2014, 07:20

... probe and a simple pulseacquire sequence (30° pulses for 1H and 31P and 90° pulse for 13C) Several 2D spectra were also recorded using standard Bruker parameters NMR data for ThDP, AThDP, ThTP and ... we report the chemical synthesis of AThTP and AThDP, their purification and their physicochemical characterization using positive ESI-MS, 1H-, 13 C- and 31P-NMR (Table 1), as well as molecular ... 500 ⁄ 125 ⁄ 202.5 MHz in D2O at pH 7.4 and 25 °C TMS and H3PO4 were used as references ThDP was a commercial preparation (Sigma-Aldrich), and ThTP, AThDP and AThTP were synthesized as described...

Ngày tải lên: 07/03/2014, 01:20

... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged ... expression vectors and USP7 cDNA as well as Shirley Gil-Parrado, Bruno Martoglio, Martin Renatus and Jorg Eder (Novartis, Basel, Switzerland) ¨ ´ for support and helpful discussions A Fernandez´ Montalvan ... Fernandez-Montalvan et al Biochemical characterization of USP7 Fig Enzymatic characterization of USP7 (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC (j), SUMO-1-AMC (d) and Nedd8-AMC...

Ngày tải lên: 07/03/2014, 05:20

... crucial for dimerization and monomer stability, in Sl00 A4 and other S100 proteins [23] (Fig 3), are clustered between helix I and IV (Fig 5B), in the same way as in Sl00 A4 and other S100 proteins ... the crystal and/ or NMR structures of other members of the family The molecule is depicted in strand representation, and a helices are numbered from I to IV (B) Residues Leu7, Arg8 and Phe11 from ... a-helix I, and Trp60, Phe63, Ala66 and Phe67 from a helix IV (located at equivalent positions to those crucial for S100 A4 dimerization and monomer stability [23]) are shown in dark and light...

Ngày tải lên: 08/03/2014, 22:20

... strands and 2, and and (Fig 9B), which play a pivotal role in macromolecular selectivity of SVSPs The catalytically important residue Asp102 is also located in this domain and precedes strand ... SVSPs (A) Cartoon and surface representations of SVSPs highlighting the two-six-stranded b-barrel structural lobes (in green and grey) The N-terminal domain contains six b-strands and a single short ... bridges Cys22 ⁄ Cys157 and Cys91 ⁄ Cys245E are included The C-terminal subdomain encompasses the sixstranded b-sheet and contains two a-helices, one inserted between strands and 9, and the other located...

Ngày tải lên: 14/03/2014, 22:20

... composed mainly of a helices (Figs and 2) The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) anking a ... similarities to classical EF-hand proteins The second pair of EF-hands in calmodulin (PDB code 1CLL) [19] and the second pair of EF-hands in troponin C (PDB code 1NCX) [20] are structurally closely related ... remodeling is essential for macrophages and Iba1 was found to bind and cross-link lamentous actin (F-actin) [11,12] and to translocate to lamellipodia, membrane rufes and phagocytic cups [3,12] Iba1...

Ngày tải lên: 16/03/2014, 06:20

... planes along the cube height and the width are 0.32 and 0.33 nm, which are in agreement with distance between (0 1) and (11 0) planes of rutile SnO2, respectively The growth direction for the cubes ... observed experimentally [25] A proposed growth mechanism A proposed growth mechanism for SnO2 micron cube-shaped crystals, in terms of chemical reactions and crystal growth is described here From the ... 3Gþ ðEu Þ 5 (1) where the A1g, B1g, A2g and Eg modes are Raman active and A2g and B1u modes are inactive In Fig 4, there are peaks at 476, 634, 659, 693, and 776 cmÀ1 in the Raman spectrum which...

Ngày tải lên: 19/03/2014, 16:48

... into SFC and FFC, SFD, FFD and FFD-500 groups SFC and SFD groups received AIN-76 basal diet, whereas FFC and FFD groups received AIN-76 diet, wherein starch was replaced with 5% wheat bran and 2.5% ... 1900 m/z and 0.3 m eluted fractions showed signals with masses of 3322 (m ⁄ z) and 3443 (m ⁄ z), respectively (Figs 4A and 5A) Similarly, in the fiber-treated diabetic group the 0.2 and 0.3 m ... of their pivotal functions in cell adhesion and recognition mechanisms, and their importance in signal transduction and as markers of differentiation and carcinogenesis [18] Hence, studying the...

Ngày tải lên: 22/03/2014, 17:20