fi cation from dried blood spots
Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
Ngày tải lên :
07/03/2014, 00:20
... mechanism of ampli cation of whole cDNAs and the TSAT-PCR technique (A) Ampli cation of full-length cDNAs (B) Procedure for the nested PCR reactions This figure is reproduced from [39] Fig Schematic ... templates for GLGI ampli cation, decreasing the complexity of the templates and thus reducing possible nonspecific ampli cation; (b) the 3¢ cDNAs are enriched by PCR to provide sufficient templates for ... is used for GLGI ampli cation; and (e) direct precipitation of GLGI-amplified products and cloning into vectors without gel puri cation prevents the potential loss of amplified products These changes...
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Báo cáo khoa học: "Semi-Supervised Cause Identification from Aviation Safety Reports" pot
Ngày tải lên :
08/03/2014, 00:20
... acquiring classifiers More specifically, both baselines recast the cause identi cation problem as a set of 14 binary classi cation problems, one for predicting each shaper In the binary classi cation problem ... challenging text classi cation problem, cause identi cation from aviation safety reports, to the NLP community Second, we created an annotated dataset for cause identi cation that is made publicly ... number of labels associated with the reports Finally, our cause identi cation task is domain-specific, involving the classi cation of documents that all belong to the aviation domain This paper...
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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx
Ngày tải lên :
18/06/2014, 19:20
... rates of CTCs in 84 blood samples from 48 epithelial cancer patients and in 30 samples from 22 melanoma patients Carcinoma Number of blood samples Number of patients Positivity of blood samples Gastric ... cells spiked) Detection of CTCs in blood samples from cancer patients CTCs were enriched by CD45 depletion and then analyzed by flow cytometry in 84 blood samples from 48 epithelial cancer patients ... depletion and EpCAM-positive enrichment) a total of 15 blood samples from healthy volunteers were also analyzed CD45 Depletion Method Red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO and...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
Ngày tải lên :
18/06/2014, 19:20
... peripheral blood cells are analyzed by routine flow cytometry for the presence of myeloid-derived suppressor cells (MDSC) as a marker for tumor presence Active MDSC are distinguished from normal blood ... data from an on-going clinical study in our laboratory suggests that MDSC detection in peripheral blood using definitive biomarkers for CD33+ and CD11b+ subsets can distinguish cancer patients from ... collected from tumorPBMC co-cultures Adherent cells were removed using the non-protease cell detachment solution Detachin (GenLantis, San Diego, CA) Myeloid cells were then isolated from the co-cultures...
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báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx
Ngày tải lên :
20/06/2014, 03:20
... rates of CTCs in 84 blood samples from 48 epithelial cancer patients and in 30 samples from 22 melanoma patients Carcinoma Number of blood samples Number of patients Positivity of blood samples Gastric ... cells spiked) Detection of CTCs in blood samples from cancer patients CTCs were enriched by CD45 depletion and then analyzed by flow cytometry in 84 blood samples from 48 epithelial cancer patients ... depletion and EpCAM-positive enrichment) a total of 15 blood samples from healthy volunteers were also analyzed CD45 Depletion Method Red blood cell lysis buffer (154 mM NH4Cl, 10 mM KHCO and...
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Báo cáo y học: " Co-receptor usage and prediction of v3 genotyping algorithms in hiv-1 subtype b’ from paid blood donors experienced anti-retroviral therapy in chinese central province" pps
Ngày tải lên :
12/08/2014, 01:22
... isolates Page of HIV-1 isolation from patients’ PBMCs Primary HIV-1 strains were isolated by co-culturing PBMCs from infected individual and those from two or more from healthy individuals after ... usage is not enough for HIV-1 B’ subtype from patients experienced ART Methods Study population All the subjects were recruited from HIV-1 infected former blood/ plasma donors (FBDs)[13] in the central ... treatment was selected from Anhui province, who were recruited as cohort study of CIPRA (Comprehensive International Program of Research on AIDS) in 20052007 The blood from all the subjects was...
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Báo cáo khoa học: "Use of dried blood samples for monitoring hepatitis B virus infection" ppsx
Ngày tải lên :
12/08/2014, 04:20
... Stability the dried blood samples Stability of the dried blood samples PCR amplifications of HBV DNA from DBS 1% Agarose gel showing a 322 bp fragment of the HBV-"a determinant" amplified from DBS ... K: Extraction of RNA from dried blood on filter papers after long-term storage Clin Chem 2003, 49:979-981 Zhang YH, McCabe ER: RNA analysis from newborn screening dried blood specimens Hum Genet ... genotyping from dried blood spots stored for year at degrees C J Antimicrob Chemother 2008, 61:1217-1220 Mwaba P, Cassol S, Nunn A, Pilon R, Chintu C, Janes M, et al.: Whole blood versus plasma spots...
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Báo cáo y học: "Predicting iron and folate deficiency anaemias from standard blood testing: the mechanism and implications for clinical medicine and public health in developing countries" ppt
Ngày tải lên :
13/08/2014, 23:20
... for another 30 days with normal haempoiesis From Day 10 to Day 40, haemopoiesis shifts from normal to the macrocytic pattern of folate deficiency From Day 40 onwards, the folate deficiency has ... other clinical applications for routine blood analysis The model shows that in iron deficiency, which could be due to inadequate iron stores or unavailability of iron resulting from acute infection, ... normal blood When iron is deficient the hitherto unexplained changes appear The model also explains why people with macrocytic anaemia may have normal folate level even though the changes in the blood...
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Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx
Ngày tải lên :
16/03/2014, 05:20
... and blood ow retardation by dilating blood vessels It also induces plasma diapedesis into tissue by increasing vascular permeability, leading to blood condensation and blood ow retardation in blood ... act under normal conditions However, once insect blood- sucking injures blood vessels, localized increase of free Zn2+ may occur; Zn2+ is released from the cytoplasm of the injured cells into the ... constructed from the isolated mRNA using the SuperScript plasmid system (Gibco BRL, Gaitherburg, MD) In total, 550 cDNA clones were picked randomly from this library, and plasmids were puried from overnight...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
Ngày tải lên :
18/02/2014, 08:20
... synergies I Fendri et al Table Identi cation of specific components detected in fractions F1, F5 and F6 using MS analysis All identifications were based on peptide mass fingerprint analyses using the MALDI-TOF ... tolerance of 150 p.p.m., one missing cleavage allowed, a fixed modi cation of cysteines by iodoacetamide (carbamidomethyl), a variable modi cation of methionines (oxidation) and N-term glutamine ... oxidation, cysteine carbamidomethylation and no fixed modi cation, and 1.5 and 1.0 Da as the maximum precursor and fragment tolerance Positive identi cation of peptides was assessed by a cross-correlation...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
Ngày tải lên :
18/02/2014, 16:20
... report the identi cation of SmSmad1B cDNA and present its gene structure along with the expression profiles, immunolocalization, and protein interaction properties Results Identi cation of SmSmad1B ... to evaluate the efficacy of the primer pairs used and to confirm that the collected data correspond to a single ampli cation product for each gene Production of SmSmad1B-specific antiserum and western ... preimmune rabbit sera were purified over protein G Sepharose (GE Healthcare Biosciences) to isolate the IgG fractions Specific anti-SmSmad1B IgG was isolated by affinity puri cation over SmSmad1B linker...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
Ngày tải lên :
18/02/2014, 16:20
... n=4 RpCAbr amplification normalized with 18S amplification RpCAtr amplification normalized with 18S amplification Fig Normalized amplifications of RpCAbr and RpCAtr with 18S ampli cation The number ... calculate the PCR efficiencies, which are critical for correct quanti cation Carbonic anhydrase transcripts in Riftia Data analysis For each transcript, the efficiency (E) was calculated from the slope ... quantity of amplified cDNA during the exponential phase) Then, for relative quanti cation measurement, we used the 2–DDCt method [38] For each transcript, the relative quanti cation result was...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
Ngày tải lên :
18/02/2014, 17:20
... events initiate blood clotting and fibrin dissolution simultaneously in a process called intrinsic or internal fibrinolysis [15], plasmin is generated directly on the surface of fibrin fibers and so ... of fibrin architecture during fibrin formation and intrinsic fibrinolysis of fibrin-rich clots J Biol Chem 278, 21331–21335 16 Schneider M & Nesheim M (2004) A study of the protection of plasmin from ... that interaction with kringle is sufficient for their action This finding is in agreement with an earlier report that oleic acid binds to kringle with an affinity that is an order of magnitude higher...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
Ngày tải lên :
18/02/2014, 17:20
... peptide chain from the l-configuration to the d-configuration, was first identified in dermophin, an opiate-like peptide from the skin of the Table D-Amino acid-containing peptides from different ... peptide from the Australian platypus [11], fulicin from African giant snails [12], and contryphan from cone snails (Table 2) The first d-residue containing conotoxin, contryphan-R, was purified from ... this difficult modi cation at different positions This is not surprising, because of the well-known high content of post-translational modifications in conotoxins [8] It is noteworthy that from the...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... & Duchiron, F (1998) Puri cation and properties of a thermoactive and thermostable pullulanase from Thermoccocus hydrothermalis, a hyperthermophilic archaeon isolated from deep sea hydrothermal ... a retaining b-1,4-glycanase from Cellulomonas fimi Biochemistry 35, 13165–13172 Cobucci-Ponzano, B., Trincone, A., Giordano, A., Rossi, M & Moracci, M (2003) Identi cation of the catalytic nucleophile ... H.C., Lewis, M.S., Griko, Y.V., Privalov, P.L & Anfinsen, C.B (1993) The puri cation and characterization of an extremely thermostable a-amylase from the hyperthermophilic archaebacterium Pyrococcus...
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Tài liệu Báo cáo khoa học: "Mining User Reviews: from Specification to Summarization Xinfan Meng Key Laboratory of Computational Linguistics " doc
Ngày tải lên :
20/02/2014, 09:20
... in Figure We first retrieve multiple specifications from various sources like websites, user manuals etc Then we run clustering algorithms on the specifications and generate a speci cation tree And ... output The specifications for all kinds of products are retrieved from ZOL, PConline and IT168 websites We run the clustering algorithm on the specifications and generate a speci cation tree for ... features are not available in all specifications For instance, “WiFi” features are only available in a few mobile phones specifications Also, different specifications might express the same features...
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Tài liệu Báo cáo khoa học: "Machine Learning for Coreference Resolution: From Local Classification to Global Ranking" ppt
Ngày tải lên :
20/02/2014, 15:20
... the RIPPER rule learning algorithm (Cohen, 1995), and maximum entropy classi cation (Berger et al., 1996) The classi cation model induced by each of these learners returns a number between and ... coordinate the coreference classi cation decisions Selecting a coreference system, then, is a matter of instantiating these elements with specific values Now we need to define the set of allowable values ... feature set, coordinating the classi cation decisions via closest-first clustering Ng and Cardie’s system, on the other hand, employs RIPPER to train a coreference classifier on instances created by N&C’s...
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Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Purification and properties docx
Ngày tải lên :
20/02/2014, 23:20
... xanthine oxidase is given The amounts of purified FDH1 used for isolation of the pterin cofactor were not sufficient to allow quanti cation or the identi cation of the exact nature of the molybdopterin ... subunits homologous to the subunits of FDH1 were also identified in the Methylococcus Puri cation of NAD-dependent formate dehydrogenase and identi cation of encoding genes Cell extracts of M extorquens ... during the puri cation were depleted of oxygen by boiling for followed by cooling under vacuum with stirring, and the addition of mM dithiothreitol All of the chromatographic puri cation steps...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
Ngày tải lên :
20/02/2014, 23:20
... pooled as the purified HdEG66 Ó FEBS 2003 774 K.-i Suzuki et al (Eur J Biochem 270) Table Puri cation of cellulase from abalone Haliotis discus hannai One unit of cellulase was defined as the amount ... reducing sugars equivalent to 1.0 lmol of glucose per Puri cation step Total protein (mg) Specific activity (unitsÆmg)1) Total activity (units) Puri cation (fold) Yield (%) Crude extract CM-III fraction ... DNA sequencer (Applied Biosystems, CA, USA) Results Puri cation of cellulase from abalone hepatopancreas Hepatopancreas (125 g) dissected from 10 abalones (average shell size: · cm) were cut into...
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