auxin mode of action at cell membrane and cell wall
Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... Hiromi K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions Evaluation of subsite afnities of exo-enzyme Biochem Biophys ... 8.0 The cell suspension was sonicated (3 ã 15 s), and cell debris was removed by centrifugation at 16 000 g for 10 The resulting supernatant was incubated for 20 at 80 C, and precipitated proteins ... basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent of the length of the substrate, Km and Vmax were used to calculate...
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Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... together with potential implications for the current understanding of its mode of action The cagPAI and the Cag type IV secretion apparatus Gene arrangement and variants of the cagPAI The cagPAI, ... phosphorylation of its effector protein CagA into various host cells is a hallmark of CagT4SS activity Despite the molecular characterization of a number of effects in host cells, the exact function of ... genome island but was split as a result of integration of an IS605 insertion element and an associated genome 1204 rearrangement However, comparative sequence analysis of multiple cag islands [11–13]...
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Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... 1DG and Table S1) These results indicate that the inhibitory activity of this group of peptides is attributable to their effect on the turnover number of IN, and suggest a different mode of action ... the conformation of active and nonactive backbone cyclic analogs of substance P as a tool to elucidate features of the bioactive conformation: NMR and molecular dynamics in DMSO and water J Med ... effect of peptides on the strand-transfer reaction When the effects of the various peptides on the strandtransfer step of the IN enzymatic reaction were studied, essentially the same pattern...
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Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
... & Hodges RS (1984) Synthesis of a model protein of defined secondary and quaternary structure Effect of chain length on the stabilization and formation of two-stranded alpha-helical coiled-coils ... Sahl HG & Tossi A (2005) Controlled alteration of the shape and conformational stability of alpha-helical cell- lytic peptides: effect on mode of action and cell specificity Biochem J 390, 177–188 ... with that of M17 In addition, whereas M17P exhibited a distinct association and dissociation, M17 had a very slow dissociation at a low peptide concentration (less 20 lm) and failed to dissociate...
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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... from T viride AZ36 Mode of action in the hydrolysis of laminarins The mode of action of the exo-1,3-b-glucanase was studied using laminarin from L digitata with a ratio of 1,3-b- and Exo-1,3-b-glucanase ... mg:mL21 were incubated with 2– U of enzyme at 37 8C for various time intervals For studies of the mode of action in the hydroysis of laminarins, intermediate products were fractionated by HPLC on ... substrate, Km is 10-fold higher and the rate of hydrolysis is < twofold lower than for laminarin from L digitata The mode of action of the exo-1,3-b-glucanase on laminarin from L digitata was...
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Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
... number of common features Both are potent and selective inhibitors of bacterial RNAPs (Table 1) and cellular RNA biosynthesis (Figs and 3), both act at the level of transcription initiation (Fig ... contain alterations in cell permeability, rather than genuine RNAP mutations The isolation and sequencing of a statistically significant number of mutants could assess the extent of such phenomenon ... assayed by incubating 10 lL of plasmolyzed cells in a total volume of 50 lL of 20 mM Hepes, pH 8.0, containing 10 mM KCl, 10 mM magnesium acetate, 0.2 mM MnCl2, 0.5 mM each of ATP, GTP and CTP, 10...
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stenersen - chemical pesticides - mode of action and toxicology (crc, 2004)
... Herbicides that inhibit synthesis of amino acids 81 5.2.1 The mode of action of glyphosate 81 5.2.2 Degradation of glyphosate 83 5.2.3 Selectivity 84 5.2.4 Mode of action of ... interpretation problems will be formidable 20 2.2.2 Chemical pesticides: Mode of action and toxicology Dose and effect The law of mass action tells us that the amount of reaction products and the ... Motivation The mode of action of pesticides is extremely fascinating because the subject covers so many fields of biology and chemistry and has many practical implications All disciplines of biology...
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HERBICIDES – MECHANISMS AND MODE OF ACTION ppt
... Phe-403 has rotated away from the inhibitor 14 Herbicides – Mechanisms and Mode of Action The interaction between the β-triketones and the catalytic site of AtHPPD was modeled by docking of inhibitors ... triphosphate (ATP), phosphate (Pi), and pyruvate with adenine monophosphate (AMP), pyrophosphate (PPi), and phosphoenolpyruvate (PEP) in the presence of magnesium and potassium/sodium ions (Mg2+ and ... herbicides regulate plant growth at tissue and cellular level Herbicides with the same mode of action generally exhibit the same translocation pattern and produce similar injury symptoms At the physiological...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 2 pptx
... example, we can use Parathion oil® and Bladan® and suggest that they have LD50 values of 12 and 10 mg/kg, respectively A dose consisting of mg/kg of Parathion oil and mg/kg of Bladan will then kill ... with the concentrations of the reactants This means that there is always a positive relation between dose and the degree of poisoning A greater dose gives a greater concentration of the toxicant ... than death, we use the term ED50 (effective dose in 50% of the population), and if we study concentrations and not doses, we use the terms LC50 (lethal concentration in 50% of the population) and...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 3 pps
... minor pathway that reduces nitrate to ammonia at the outer membrane surface Some important herbicides (paraquat and diquat) can snatch the electrons before the delivery to ferredoxin and generate ... they have a related mode of action They react with tubulin, a protein that is the building block of the intracellular skeleton in eukaryotic cells The shape and structure of a cell are dependent ... purposes At least some of them owe their mode of action to the inhibition of ATP synthase in the target organism Tricyclohexyltin (cyhexatin) and azocyclotin are used as selective acaricides Cyhexatin...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 4 ppt
... engineered plants that produce them, results in cessation of feeding of Lepidoptera larvae and paralysis of the gut that retards the passage of food and allows the spores to germinate The larvae suffer ... freely pass This model proposes that an influx of water, along with ions, results in swelling and lysis The epithelium is destroyed and the insect rots Consumption of food treated with the endotoxins, ... Diptera It is thermostable and is not destroyed by heating at 70˚C for 15 minutes Because of the vertebrate toxicity, most commercial preparations use subspecies or isolates that not produce β-exotoxin...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 5 docx
... pressure and can poison insects and vertebrates through the respiratory system Many molecular modifications of parathion were tested at an early stage of the development of organophosphates Aldridge and ... in some types of nonneural cells of vertebrates Recent data indicate that NTE is involved in a cell- signaling pathway controlling interactions between neurons and accessory glial cells in the ... chloroplasts Naturally the reactions involved in the synthesis of 5-enolpyruvoyl-shikimate-3-phosphate and its inhibition by glyphosate have been studied extensively, and many thousands of publications...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 6 ppsx
... mechanism is of importance in understanding the mode of action of some pesticides The passage of a nerve impulse at a point on the axon is associated with a sudden drop, and even reversal, of the voltage ... Second World War One of the popular hypotheses promoted by Dr Hubert Martin was that DDT satisfied three requirements: Ability to penetrate and concentrate at the site of action Adequate stability to ... binds at the receptor site that is located on a part of the receptor molecule at the outside of the membrane, a cascade of chemical reactions is started on the inside There are several types of...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 8 pptx
... the vapor densities of p,p-DDT on sand at 20, 30, and 40˚C and found them to be 2.9, 13.6, and 60.2 ng/l Furthermore, Spencer and Cliath found that DDE and other derivatives of DDT have a higher ... concentrate on rats and other vertebrates Valuable data on pesticide biotransformation are also found in Hayes and Laws (1991a and b), Miyamato et al (1988), and Rockstein (1978) Chambers and Yardbrough’s ... two steps (reactions and 8) Reactions and can also be catalyzed by a group of enzymes called glutathione peroxidases, whereas reaction is catalyzed by glutathione reductase The glutathione transferases...
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Chemical Pesticides: Mode of Action and Toxicology - Chapter 9 pdf
... this reaction: Cl N N GS NHCH2CH3 N NHCH(CH3)2 atrazine GSH N N NHCH2CH3 N NHCH(CH3)2 conjugate of atrazine and glutathione The data in Table 9.4 are extracted from data of Plaisance and Gronwald ... detoxication in plants Atrazine has an electrophilic carbon that may be attacked by glutathione, and biotypes of various weeds may have glutathione transferases that have a better ability to catalyze ... substrate concentration at which an enzyme-catalyzed reaction proceeds at one-half its maximum velocity.) This indicates a somewhat less efficient enzyme, but the difference is so slight that it...
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Báo cáo khoa học: "Anti-viral properties and mode of action of standardized Echinacea purpurea extract against highly pathogenic avian Influenza virus (H5N1, H7N7) and swine-origin H1N1 (S-OIV)" docx
... 0.001) and MDCK cells were pre-incubated with EF at the indicated concentrations hour prior to infection Infected cells were then incubated in media with EF at the appropriate concentrations ... amount and the localization of RNPs detected in cells infected with pre-treated IV was the same as for untreated cells infected with untreated virus It should be noted that the treatment of infected ... p.i Treatment Pre-incubation virus - - + + - + - Pre-incubation cells - - + - + - + Incubation of cells p.i - + + + + - - Figure Pre-treatment of IV with EF is most effective Pre-treatment of IV...
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Molecular mechanisms of mechanosensing at cell cell and cell matrix adhesion 2
... Altered cell- cell and cell- matrix adhesion function is one of the hall marks of cancer [49, 50] The proper functions of cell adhesions, both in terms of adhesion strength and underlying signaling pathways ... conformation changes At subcellular level, vinculin plays important roles in regulating cell- matrix and cell- cell adhesions Cells lacking vinculin develop less stable focal adhesions and migrate ... observation, a new model has been proposed that the interaction between β-catenin and α-catenin acts as a means of enrich α-catenin to the adherens junctions Then α-catenin dissociate from β-catenin,...
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Agents for hepatocellular carcinoma synthesis and mode of action
... curves of determination of (A) IC50 and (B) GI50 of 5-9 on HuH7 cells, 72 h incubation Figure 3-2: Dose response curves of determination of (A) IC50 and (B) GI50 of 3-12 on HuH7 cells, 72 h incubation ... Intensity of blots obtained from (A) untreated HuH7 cells and HuH7 cells treated with (B) 47 at 10 µM, (C) 3-12 at 0.5 µM and (D) 3-12 at µM Figure 5-4: (A) 3-12 reduced the phosphorylation of HER3 at ... deprivation Forming mutual loop of regulation of cell proliferation and cell cycle Mitigating the BAX-dependent intrinsic apoptosis pathway Maintaining genomic stability Regulation of fatty acid...
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Investigations into the chemopreventive potential of stilbenes, indolinones and isoindigos synthesis and mode of action studies
... balance of activities of enzymes that promote the formation of reactive intermediates and those that detoxify these reactive species One notable feature of phase II enzymes is that they are often ... gluronidation and sulfation reactions that are responsible for the poor bioavailability of resveratrol Second, notwithstanding the many methoxy analogues of resveratrol that have been synthesized and ... to be fully elucidated A great deal more is known of the exogenous ligands of AhR, most of which are halogenated and polycyclic aromatic hydrocarbons One of its most potent ligands is 2,3,7,8tetrachlorodibenzo-p-dioxin...
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Antiproliferative activity of curcumin analogs on acute promyelocytic leukemia synthesis and mode of action studies
... regulator of the growth and maturation of cells of myeloid lineage.11-13 It also plays a role in the differentiation of thymocytes and cells of erythroid lineage 14 N-CoR is involved in the pathogenesis ... growing cells that are immature and undifferentiated, whereas chronic leukemic cells are slow growing and largely differentiated A systematic classification of the various classes and subtypes of ... synthesized and evaluated on two APL cell lines (NB4 and NB4-R1), two leukemic non-APL cell lines (HL60 and K562) and two nonmalignant cell lines (IMR90 and MCF10A) An assessment of structural features...
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Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25