λ by motor dna complex assembly
Báo cáo khoa hoc:" DNA-nanostructure-assembly by sequential spotting Journal of Nanobiotechnology" pot
Ngày tải lên :
11/08/2014, 08:20
... positions by retraction Ink was supplied to the tip by a hypodermic needle of Popper & Sons, Inc (N.Y 11040 USA) DNA preparation The DNA- construct was generated by digesting 10 µg M13mp18 RF I DNA ... that connects the spots DNA is visualized by SYBR-Green I Figure - Sequence of the homopurine DNA target PNA strand bound to double stranded DNA by forming a triplex invasion complex For better solubility ... array-preparation DNA- construct side A and B self -assembly was initiated by transferring a diluted mixture of side A and side B DNA- constructs directly onto the array A complete DNA bridge has been...
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
Ngày tải lên :
19/02/2014, 06:20
... verified by DNA sequencing (ABI PRISM377 DNA sequencer; PerkinElmer Life Science, Wellesley, MA) Parental TMK-1 cells were transfected overnight with the bcl-XL vector or the empty pcDNA3.1 vector by ... expression vector pcDNA3.1 Plasmid DNA was prepared by standard techniques (QIAGEN Plasmid Midi Kit; Hilden, Germany) pBabePuro, a puromycin-resistant vector, was kindly provided by Dr K Shuai (University ... indicated times and used for the DNA fragmentation assay as described in Experimental procedures pretreatment (Fig 5C) By contrast, JunD expression was not induced by EGF treatment (data not shown)...
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Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx
Ngày tải lên :
21/02/2014, 00:20
... inhibited by ndsDNA [20] In our case, at low [ndsDNA] and high [ATP], the opposite situation may arise, when ATP inhibits binding of ndsDNA and subsequent ligation by occupying the dsDNA-binding ... mixture by the concentration of T4 DNA ligase To study the T4 DNA ligase-promoted end-joining reaction, we used synthetic DNA oligonucleotides as described in Experimental procedures Two ndsDNA substrates ... binding of ATP is faster than binding of DNA (102 s)1 for DNA vs 4.5 ã 103 s)1 for ATP at lM ndsDNA and mM ATP) Modeling studies indicate that ndsDNA in complex with ligase hinders the access of...
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Structure of the S15,S6,S18-rRNA Complex:Assembly of the 30S Ribosome Central Domain
Ngày tải lên :
13/03/2014, 19:08
... S6 weakly associates with the S15-RNA complex that serves as a scaffold for cooperative folding and assembly of S18 The subsequent steps in central domain assembly, consistent with the available ... S18) as described (13) Crystals of the Tth T4 RNP were grown at room temperature to 0.5 mm by 0.2 mm by sitting-droplet vapor diffusion methods from 1.8 M (NH4)2SO4, 20 mM MgCl2, and 50 mM Kϩcacodylate ... (30) Heavy-atom derivative sites were confirmed by Patterson and difference Fourier methods with XtalView (31) Experimental phases were obtained by MIR to 3.5 Å with Mlphare (32) using seven heavy-atom...
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micelle-assisted synthesis of polyaniline magnetite nanorods by in situ self - assembly process
Ngày tải lên :
20/03/2014, 13:04
... micelleassisted synthesis by in situ self -assembly method The presence of a high concentration of DBSA can effectively prevent the formation of larger particles, thereby producing nanometersized ... mechanism of the composite particles is shown in Fig By taking advantage of the micelle reaction system, we firstly obtained colloidal PANI doped with DBSA by the one-step chemical oxidative polymerization ... particle sizes were controlled by the micelle “reactor” sizes [26] The presence of a high concentration of DBSA can prevent the formation of larger particles, thereby only producing nanometer-sized...
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Báo cáo khoa học: Template-independent ligation of single-stranded DNA by T4 DNA ligase doc
Ngày tải lên :
23/03/2014, 11:20
... substrate, and 10 U DNA ligase (Weiss units in the case of T4 DNA ligase) at 16 °C (T4 DNA ligase and E.coli DNA ligase) or 45 °C (Taq DNA ligase and 5997 Ligation of ssDNA by T4 DNA Ligase Ampligase) ... Ligation of ssDNA by T4 DNA Ligase nontemplated ligation on ssDNA substrates [29,30], this property of T4 DNA ligase has, to our knowledge, not been reported Unlike T4 DNA ligase, bacterial DNA ligases ... ssDNA by T4 DNA Ligase H Kuhn and M D Frank-Kamenetskii A B Fig Investigation of ssDNA ligation activity of different DNA ligases Oligonucleotide I (10 nM) was incubated with ligase, cleaved by...
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Báo cáo Y học: The cytoplasmic C-terminus of the sulfonylurea receptor is important for KATP channel function but is not key for complex assembly or trafficking pdf
Ngày tải lên :
31/03/2014, 08:20
... the myc epitope followed by a stop codon and an AvrII site The native fragment was replaced by this cassette and resulting product subcloned into the XhoI/XbaI sites of pcDNA3 using a SalI/SpeI ... and Kir6.2mycHis6 was expressed in pcDNA3.1/Zeo (Invitrogen) Kir6.2mycHis6 was generated from a previous study [15] The sequence of all mutants was confirmed by DNA sequencing using the dRhodamine ... period, the binding resin was pelleted by centrifugation (20 000 g for at °C) followed by five washes with mL binding buffer Bound protein was recovered by incubation with 80 lL · Laemmli gel...
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Báo cáo hóa học: " Computer-aided design of nano-filter construction using DNA self-assembly" docx
Ngày tải lên :
22/06/2014, 22:20
... after removing a self-assembled resist pattern by AFM Some other scientists have been bound DNA to metal substrates using DNA end modifications [24–29] By using this feature and designing the sequences ... junctions on the top-right are complexes in parallel orientation to yield the structure on the bottom Note that the complex has maintained open valences, so it could be extended by the addition of more ... to direct branched molecules to associate by using sticky ends This idea is shown in Fig 1b What this figure shows is a self -assembly process directed by the complementary sequences on the sticky...
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Báo cáo khoa học: "Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens" pps
Ngày tải lên :
07/08/2014, 18:21
... snietorp 3PV dna ,4PV ,2PV eht gnidocne ,342PV-ANDcp ,eniccav AND ehT sdimsalp fo noitaraperp dna noitcurtsnoC doirep latnemirepxe eht gnirud mutibil da retaw dna doof htiw dedivorp dna erusserp ... ralucelom a htiw dnab a dna ,)1 giF( metsys noitceted dna noitalsnart /noitpircsnart ortiv ni na gnisu detceted saw γ-NFIhC-ANDcp yb desserpxe nietorp ehT iloc E la te noitalsnart dna noitpircsnart ... htiw dna γ-NFIhc 8.4 gnidocne dimsalp dna 342PV-ANDcp htiw :γ-NFIhc sulp eniccav AND ;egnellahc eht htiw dna NDO-GpC htiw dexim 342PV-ANDcp htiw :NDO -GpC sulp eniccav AND ;egnellahc eht htiw dna...
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Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
Ngày tải lên :
09/08/2014, 09:20
... design DNAdirected alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA- directed ... irradiation was obtained by the survival fractions measured by separate treatment as reported previously [21] The expected effect by two separate treatments was determined by the formula SF(Drug) ... http://www.ro-journal.com/content/6/1/7 DNA alkylating agents are used widely for treatment of a variety of pediatric and adult cancers because the cytotoxic effects of these agents can directly modify DNA and cause DNA lesions...
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Transcription-coupled mutagenesis by the DNA deaminase AID Erik D Larson and Nancy Maizels ppt
Ngày tải lên :
09/08/2014, 20:20
... infection by specifically deaminating the first-strand cDNA synthesized by reverse transcriptase (reviewed in [15]) The human immunodeficiency virus HIV-1 evades this protective mechanism by expressing ... deposited research Knowing that AID induces transcription-coupled mutagenesis by deaminating C to U in DNA, and that uracil -DNA glycosylase removes U to produce an abasic site (see shaded box in Figure ... single-stranded DNA but requires the action of RNase Proc Natl Acad Sci USA 2003, 100:4102-4107 Chaudhuri J, Tian M, Khuong C, Chua K, Pinaud E, Alt FW: Transcription-targeted DNA deamination by the AID...
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Báo cáo y học: "proving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps" docx
Ngày tải lên :
09/08/2014, 20:21
... and any existing assembly by mapping, and then improves the assembly by localised assembly of reads at real gaps and regions that were previously unresolved in the assembly; and it uses iterative ... improved assembly against the finished sequence of S enterica As illustrated by Figure 4, the improved assembly and the reference sequence are in good agreement without obvious signatures of misassembly ... generate the original de novo assembly This is because each k-mer is more unique in a local rather than a whole genome assembly In generating the whole genome assembly, the assembly software (in our...
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Báo cáo y học: "Drug efficacy by direct and adjusted indirect comparison to placebo: An illustration by Mycobacterium avium complex prophylaxis in HIV" potx
Ngày tải lên :
10/08/2014, 05:22
... azithromycin derived by adjusted indirect comparison were not significantly different from the efficacies derived by direct comparison However, our estimate of the efficacy of rifabutin by indirect comparison ... Cite this article as: Chu et al.: Drug efficacy by direct and adjusted indirect comparison to placebo: An illustration by Mycobacterium avium complex prophylaxis in HIV AIDS Research and Therapy ... Mycobacterium avium complex infection in AIDS N Engl J Med 1993, 329(12):828-33 14 Pierce M, et al: A randomized trial of clarithromycin as prophylaxis against disseminated Mycobacterium avium complex infection...
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Báo cáo y học: " Protection against H1N1 influenza challenge by a DNA vaccine expressing H3/H1 subtype hemagglutinin combined with MHC class IIrestricted epitopes" ppsx
Ngày tải lên :
11/08/2014, 21:21
... from the HA-based DNA vaccine by immune sera (A) pV-H3HA detected by H3+ serum, (B) pV-H1HA1 detected by H1+ serum, (C) pV-H3-H1 detected by H3+ serum, (D) pV-H3-H1 detected by H1+ serum, (E) ... pV-H3-EHA-H1 detected by H3+ serum, (F) pV-H3-EHA-H1 detected by H1+ serum, (G) pV-H3-EHA-H1 detected by H5+ serum (H) pV-H3-EHA-H1 detected by H7+ serum, (I) pV-H3-EHA-H1 detected by H9+ serum, (J) ... immunogenicity of the vaccines, the four DNA vaccine constructs (Figure 2) were confirmed by sequencing Protein expression from these constructs were also verified by transfection of pV-H3HA, pVH1HA1,...
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Báo cáo sinh học: "Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein" doc
Ngày tải lên :
14/08/2014, 19:22
... determined by spectrophotometry at λ = 260 and 280 nm by using the Gene Quant II apparatus (Pharmacia Biotech, Buckinghamshire, UK) Immunization procedures Normal and restricted groups were immunized by ... adopted by the Brazilian College of Animal Experimentation (COBEA), being the experimental protocol approved by the local Ethics Committee Hematological parameters Blood samples were collected by ... [15] Our group has been working with DNA vaccines constructed by inserting the heat shock protein 65 gene from Mycobacterium leprae (hsp65) into plasmid vectors (DNAhsp65) Theoretically, this construction...
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Báo cáo y học: "A TATA binding protein regulatory network that governs transcription complex assembly" pdf
Ngày tải lên :
14/08/2014, 20:22
... example, TBP) binds to DNA (D) located in the promoter of gene 'y' to form a protein -DNA complex (PxDy), as demonstrated previously PxDy + Pz → PxDyPz exemplified by a TBP DNA complex binding the ... NC2 binds to a TBPDNA complex and blocks PIC assembly [25,26] Mot1 uses the energy of ATP hydrolysis to dissociate TBP from DNA [11,27,28] Mot1 can also dissociate TBP from DNA in the presence ... are a balance of assembly and disassembly processes that typically move along different pathways Thus, PIC disassembly and RNA degradation not proceed by an exact reversal of PIC assembly and transcription...
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Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
Ngày tải lên :
09/09/2015, 11:16
... regulated by the RNA-induced silencing complex (RISC), which is a miRNA- and Argonaute protein-containing ribonucleoprotein [11] The RISC complex is guided to its target by the miRNA within the complex ... RNA-induced silencing complex Surface enhanced Raman scattering xiv siRNA SNPs ssDNA TBE Small interfering RNA Single nucleotide polymorphisms Single strand DNA Trisborate-EDTA TEM TFs λmax Transmission ... G-quadruplexinduced nanoassembly process Through the use of poly-G DNA, quadruplex formation, and modulation by molecular hairpins, AuNP assemblies was achieved and then optimized This was followed by the proof...
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Micro and nano structured functional materials by polymer aided self assembly
Ngày tải lên :
12/09/2015, 08:19
... out, line -by- line, by scanning a high-energy beam of electrons or ions over a resist material The resolution of electron beam lithography is limited by high-energy secondary electrons and by scattering ... honeycomb arrays of DNA / amphiphile complexes, saccharide-containing vinyl polymers, electrically conducting polythiophene complexes, or photoresponsive azobenzenecontaining complexes, all of ... decorated by the nanocrystals were assembled.51 In contrast to this single step assembly, copolymer-metal nano-structures were fabricated using a method in which one level of self -assembly guides...
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NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity
Ngày tải lên :
12/10/2015, 17:35
... MRN complex is explained by the multiple enzymatic and non-enzymatic activities of its components The MRE11 component is a nuclease with ssDNA endonuclease, 3’ to 5’ ssDNA exonuclease, dsDNA ... sensing DNA damage response begins with “sensor” proteins that sense DNA lesions/chromatin alterations after DNA damage induction This process is characterized by rapid formation of DNA damage ... damage sites, thus forming the functional MRN complex In the second step, MRN complex switches to a mode of direct association with damaged DNA by the DNA binding region within MRE11/RAD50 (Tauchi,...
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NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity
Ngày tải lên :
26/11/2015, 22:49
... MRN complex is explained by the multiple enzymatic and non-enzymatic activities of its components The MRE11 component is a nuclease with ssDNA endonuclease, 3’ to 5’ ssDNA exonuclease, dsDNA ... sensing DNA damage response begins with “sensor” proteins that sense DNA lesions/chromatin alterations after DNA damage induction This process is characterized by rapid formation of DNA damage ... damage sites, thus forming the functional MRN complex In the second step, MRN complex switches to a mode of direct association with damaged DNA by the DNA binding region within MRE11/RAD50 (Tauchi,...
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