δ valerolactone poly ethylene glycol poly δ valerolactone vev copolymer
Báo cáo khoa hoc:" Genetic polymorphisms and susceptibility to lung disease" ppsx
Ngày tải lên :
11/08/2014, 08:20
... http://www.jnrbm.com/content/5/1/5 [27] The TLR2 polymorphism R753Q [28] and the R677W polymorphism in humans [29-31] have been shown to be associated with increase risk for tuberculosis infection The R753Q polymorphism was ... and control groups The TLR2 polymorphism R753Q, associated with tuberculosis, was not shown to be different between the control or lung disease group The TLR2 R677W polymorphism, also associated ... importance of Toll-like receptor polymorphisms in severe infections Clin Infect Dis 2005, 41 Suppl 7:S408-S415 Bochud PY, Hawn TR, Aderem A: Cutting edge: a Toll-like receptor polymorphism that is associated...
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Báo cáo khoa học: "Expression and characterization of the flavoprotein domain of gp91phox" potx
Ngày tải lên :
07/08/2014, 14:22
... described in Materials and Methods The purified proteins were subjected to SDS-PAGE on a 12% (w/v) polyacrylamide gel, and were visualized by Coomassie-Blue staining The apparent molecular sizes ... 2) The results imply that the 22 Choon-Hoon Han and Mun-Han Lee recombinant gp91phox exists in a polymerized state in ordinary buffer even in the presence of detergent unless reducing agent is ... renaturation The observation of a stoichiometry of FAD binding to protein near : suggests that despite its polymeric state, most of the flavoprotein domain is in an active form Fig NADPH-dependent NBT reductase...
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Báo cáo y học: "A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity" doc
Ngày tải lên :
09/08/2014, 10:21
... reduce the severity of pristane-induced arthritis (an arthritis model) [20] A single nucleotide polymorphism (SNP) in the Ncf1 gene was found to be responsible for the protective effect [21] Ncf1 ... an important regulator of the immune system Materials and methods Selection of single nucleotide polymorphism The HapMap genome browser [32] was used as the primary source for selection of SNPs ... on several criteria; the minor allele frequency should be more than 5% in the Centre d'Etude du Polymorphisme Humain (CEPH) or other European cohort, the SNP should be located in a nonrepetitive...
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Báo cáo y học: "DEK binding to class II MHC Y-box sequences is gene- and allele-specific" pptx
Ngày tải lên :
09/08/2014, 01:23
... associated with predisposition to autoimmune disease [23–27], the Y box contains a single-nucleotide polymorphism that reduces sequence identity to of 10 positions We hypothesized that DEK could bind ... in the promoter regions of several class II MHC genes, and that gene- and allele-specific Y-box polymorphisms could affect DEK binding activity In this study, we examine the characteristics of ... described [28,29] Preparation of partially purified recombinant DEK protein Construction of the poly- histidine-tagged DEK bacterial expression vector is described elsewhere [16] Full-length DEK...
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Báo cáo y học: " Non-imprinted allele-specific DNA methylation on human autosomes" pptx
Ngày tải lên :
09/08/2014, 20:21
... 13 ASM, allele-specific methylation; CpG, cytosine-guanine dinucleotide; SNP, single nucleotide polymorphism Genome Biology 2009, 10:R138 http://genomebiology.com/2009/10/12/R138 Genome Biology ... some of these cases, the alleles could be differentiated by the presence of a single nucleotide polymorphism (SNP) in the sequenced region such that the sequencing reads could be sorted according ... respectively (Figure 3d), suggesting that the methylation difference is determined mainly by the polymorphisms between the alleles A similar observation was made for amplicons 23_1 and 23_2 (Additional...
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Báo cáo y học: " Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity" ppt
Ngày tải lên :
09/08/2014, 23:20
... tumor genome that provides a growth and survival advantage over other cells The single nucleotide polymorphism (SNP) array is currently one of the most efficient technologies for detecting copy ... parent-specific copy number; QC: quality control; SKY :spectral karyotyping; SNP: single nucleotide polymorphism; TAPS: Tumor Aberration Prediction Suite; TN: true negative Competing interests The ... H, Biegel JA, Maris JM: Genomic copy number determination in cancer cells from single nucleotide polymorphism microarrays based on quantitative genotyping corrected for aneuploidy Genome Res 2009,...
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Báo cáo y học: "A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients" pdf
Ngày tải lên :
10/08/2014, 21:23
... of the mutant clone [24,25] In this study, we set out to develop a single-tube allele specific-polymerase chain reaction (AS-PCR) to identify the most resistant KD mutation, T315I, in Thai CML ... mixture of μL cDNA, 2.5 mmol/L MgCl2, 0.2 mmol/L of each dNTP, 3% DMSO, and 0.625 unit of Taq DNA polymerase (Invitrogen, USA) together with 14 pmol of MT primers, pmol of WT primers, and pmol of ... amplified in a total volume of 20 μL with the following constituents, 0.2 U of high-fidelity DNA polymerase (Phusion™, FINNZYME), 5X Phusion buffer, mmol/L of MgCl , 0.2 mmol/L of each dNTPs, 10...
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Báo cáo y học: "Analysis of adenovirus trans-complementationmediated gene expression controlled by melanoma-specific TETP promoter in vitro" ppsx
Ngày tải lên :
12/08/2014, 04:20
... into the Kpn I -Bam HI (blunt)-restricted transfer plasmid pAdCMVΔlacZ-lnk1 to replace the CMV promoter The resulting pAdTETPΔlacZ-lnk1 carried an E1 deletion from bp 449 to 3323 For the generation ... (RC) Ad virus AdΔEP-TETP [40] in trans-complementationmediated expression assays For co-infection, AdCMVeGFP at MOIs of 0.37, 1.1, 3.3 and 10 were combined with RC wtAd5 and AdΔEP-TETP, or as ... melanoma RC Ad virus AdΔEP-TETP The indicated non-melanoma and melanoma cells were co-infected using AdCMV-eGFP at MOIs of 0.37, 1.1, 3.3 and 10 in combination with RC wtAd5 and AdΔEP-TETP, or the...
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Báo cáo y học: " Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene" pdf
Ngày tải lên :
13/08/2014, 05:21
... transcriptional interference occurring from elongating polymerase firing from the host gene [44], whereas work from Silicano's laboratory showed that elongating polymerase from the host gene could enhance ... through multiple proteinRNA and protein-protein interactions [13] Tat interacts with the core RNA polymerase II [14,15], the TATA-binding protein associated factor (TAFII) [16], TFIIH [17], cyclin-dependent ... Reinberg D, Peterlin BM: The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme Mol Cell Biol 1997, 17:1817-1823 Mavankal G, Ignatius Ou SH, Oliver H,...
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PHÂN BIỆT GENOTYPE 1 và 6 BẰNG kỹ THUẬT ALLELE SPECIFIC RT PCR dựa vào VÙNG GEN NS5B của VIRUS VIÊM GAN c
Ngày tải lên :
20/08/2015, 11:28
... cần nucleotide đầu mồi không bắt cặp đủ để ức chế hoạt tính kéo dài mạch khuôn Taq polymerase[5] Với đặc tính Taq polymerase với hai mồi đặc hiệu cho genotype 6, xây dựng quy trình phân type dựa ... genotype HCV; Genotype 6: cDNA genotype HCV Xây dựng quy trình phân biệt genotype Đặc điểm enzyme Taq polymerase kéo dài mạch khuôn từ vị trí mồi bắt cặp cần phải có cấu trúc bắt cặp hoàn toàn mồi mạch ... overhang đầu mồi bắt cặp với mạch khuôn ngăn cản hoạt động kéo dài mạch (primer extension) enzyme Taq polymerase Tơng tự, mồi đặc hiệu cho genotype NS5B6R bắt cặp hoàn toàn vào trình tự gen genotype...
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Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc
Ngày tải lên :
23/03/2014, 09:21
... Ala30Pro mutation in the gene encoding a-synuclein in Parkinsons’s disease Nat Genet 18, 106–108 Polymeropoulos M, Lavedan C, Leroy E, Ide S, Dehejia A, Dutra A, Pike B, Root H, Rubenstein J, Boyer...
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Nghiên cứu tính đa dạng của allele hla - DQA1 bằng kỹ thuật polymerase chain reaction sequence specific primers (PCR - SSP) ở dân tộc kinh miền trung - Việt Nam docx
Ngày tải lên :
25/03/2014, 03:22
... dNTPs dATP, dGTP, dCTP dTTP; 0,25 àM đôi mồi đặc hiệu, 0,05 àM đôi mồi đối chiếu 0,50 IU Taq DNA polymerase Tiến hành 30 chu kỳ, chu kỳ nh sau: Biến tính 940C/30 giây => giảm nhiệt 620C/30 giây ... 219 220 D Chandanayingyong, Henry A.F.Stephens, R.Claythong et al: HLA - A, -B, DQA1 and - DQB1 Polymorphism in Thais Human Inmmunology, 17, 53, 174 - 182 O Olerup, A.Aldener, A Fogdell: HLA DQB1 ... alleles in Jing nationality of Central Vietnam Methods: Applied PCR-SSP technique to determine the polymorphism of the HLA-DQA1 alleles of 214 healthy children and yoth, unrelated individuals in...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
Ngày tải lên :
18/06/2014, 15:20
... production, antigen specific cytotoxicity and lymphoproliferation assays or quantitative real-time polymerase chain reaction (qRT-PCR) for the detection of cytokine gene expression [1-3] These methods ... Expression data were calculated, as referred to βactin gene expression in each sample, by using the −ΔC t method [22] For all genes analysed, dynamic linear range of expression-detection was consistent ... specimens J Immunol 1999, 163:6867-6875 Kammula US, Marincola FM, Rosenberg SA: Real-time quantitative polymerase chain reaction assessment of immune reactivity in melanoma patients after tumor peptide...
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báo cáo hóa học:" Transcriptional regulation of bone formation by the osteoblast-specific transcription factor Osx Chi Zhang" pptx
Ngày tải lên :
20/06/2014, 04:20
... differentiation 12 reading frame (ORF) and 3-prime UTR The mouse Osx protein is a 428 amino acid polypeptide with a molecular mass of about 46 kDa as shown in Figure1 The DNAbinding domain of Osx...
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Báo cáo hóa học: " Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report" doc
Ngày tải lên :
21/06/2014, 19:20
... Majewski F: FISH studies in 45 patients with Rubinstein– Taybi syndrome: deletions associated with polysplenia, hypoplastic left heart and death in infancy Eur J Hum Genet 1999, 7:748-756 Coupry...
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