β1 in immune regulation and autoimmunity

... patients An involvement of NF-κB and AP-1 in the TNF-αinduced Cyp7b activity is in accordance with the presence of putative NF-κB and AP-1 binding sites within the Cyp7b promoter Our findings are ... assays and wrote the manuscript AK (Allard Kaptein) helped in conceiving the study and helped to draft the manuscript AK (Annemieke Kavelaars) and CH were involved in drafting and revising the ... The combined results with SN50 and PSI imply an involvement of NF-κB in TNF-α-induced Cyp7b activity MAPK inhibition did not affect the TNF-α-induced increase in Cyp7b activity We further investigated...

Ngày tải lên: 09/08/2014, 07:20

... discusses innate immune signaling and in ammatory responses to pathogens and cancer Chapters and introduce innate lymphocytes, including NKT and gamma–delta T cells and their roles in innate immune ... cancer Chapters and offer an overview of TLR- and RLR-mediated innate immune signaling and their role in detecting invading pathogens and initiating in ammatory response to pathogen infection Chapter ... pathway to induce type I interferon and in ammatory cytokines factor 88 (MyD88), TIR-domain-containing adaptor protein (TIRAP)/MyD88 adaptor-like (Mal), TIR-domain-containing adaptor inducing interferon-b...

Ngày tải lên: 05/03/2014, 23:20

... environment with financial sector standards; and (iii) encourage integration Tiering (splitting) the CFI sector into two groups, one being a large/open CFIs under banking authority supervision and another ... management, auditing, legal, personnel training and consulting The situation is illustrated in Figure The technological complexities involved in many of the inputs, the lack of economies of scale in the ... deposits and deposit like instruments, insurance and insurance like instruments, means of payments, independent of the institutions that offer the service According to this approach, regulation...

Ngày tải lên: 22/03/2014, 21:20

... determined, the binding network of fumarate in the enzyme has become clear In the fumarate-binding pocket, two arginyl residues, Arg67 and Arg91, have been identied as determining the major binding ... Fumarate-binding site for human m-NAD-ME (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3) (B) Fumaratebinding site of m-NAD-ME The corresponding amino acids in the fumarate-binding ... dimer interface [26,27] Figure 1B shows the binding mode of fumarate at the dimer interface Structural studies have revealed that Arg67 and Arg91 are the ligands for fumarate binding The side chains...

Ngày tải lên: 23/03/2014, 06:20

... dissociation of thrombin from TM, explaining the observed biphasic inhibition profiles These findings are in marked contrast to the binding of all other thrombin-binding components, and comprise yet ... type plasminogen activator inhibitor and vitronectin and evidence that the bovine inhibitor binds to a thrombin-derived amino-terminal fragment of bovine vitronectin Biochim Biophys Acta 1078, ... Latent PAI-1 was obtained by incubating 200 nM PAI-1 at 37 °C for at least 20 h Direct binding of rl-TM and solulin to VN was studied by injecting 40 lL 200 nM rl-TM, or lM solulin, in the absence...

Ngày tải lên: 23/03/2014, 17:21

... particular interest in the inflammation associated with hyperferritinemia, as is the case in hemophagocytic syndrome (HPS) and adult-onset Still's disease (ASD), because HO-1 can be involved in increased ... underlying diseases were systemic lupus erythematosus (SLE) in two; hematological malignancy, including non-Hodgkin's lymphoma, multiple myeloma, and acute myeloid leukemia, in three; and ASD and ... vein thrombus, central nervous system involvement, arterial occlusion, or gastrointestinal lesions in addition to positive CRP, indicating active disease; the other 10 were regarded as having inactive...

Ngày tải lên: 09/08/2014, 06:22

... 2009, 6:56 tries [16-19] Both macrophages and DCs perform crucial roles in linking innate and adaptive immunity and augmenting the immune response to HSV-1 infection It was previously shown that ... temperature in PBS containing 0.05% v/ v Tween-20 (PBS-T) Slides were then blocked for 30 at ambient temperature in PBS-T containing 1% w/v BSA (PBS-TB) Immunostaining was done according to a direct ... temperature in PBS-T for each, slides were dipped into ddH2O (to remove salt) and mounted in ProLong Gold mounting media containing DAPI (Invitrogen) Images were captured at 1024 × 1024 pixels (original...

Ngày tải lên: 12/08/2014, 04:21

... 2009, 6:56 tries [16-19] Both macrophages and DCs perform crucial roles in linking innate and adaptive immunity and augmenting the immune response to HSV-1 infection It was previously shown that ... temperature in PBS containing 0.05% v/ v Tween-20 (PBS-T) Slides were then blocked for 30 at ambient temperature in PBS-T containing 1% w/v BSA (PBS-TB) Immunostaining was done according to a direct ... temperature in PBS-T for each, slides were dipped into ddH2O (to remove salt) and mounted in ProLong Gold mounting media containing DAPI (Invitrogen) Images were captured at 1024 × 1024 pixels (original...

Ngày tải lên: 12/08/2014, 04:21

... administered into the coronary circulation of isolated rat hearts, and administered intravenously in canines with heart failure However, this insulinomimetic effect occurred within 30 minutes in ... for using insulin to mitigate myocardial injury in an acute setting have been limited Reasons for this limitation include problems associated with the acute use of intensive insulin therapy in patients ... tests in patients with coronary artery disease while decreasing post-test myocardial stunning, and reduce the need for insulin and inotropic support following coronary artery bypass grafting (94,...

Ngày tải lên: 24/08/2014, 12:16

... against inflammatory insult during stress 1.2 Inflammatory stress response involving HSP70 HSP70, as mentioned above, plays a role in the regulation of signaling pathways that are involved in ... is a transcription factor involved in the regulation of inflammation and cytokine production NFkB exists in an inactive form bound to IkBa in the cytoplasm The binding of IkB to NFkB prevents ... contains the calcium and membrane binding sites and is the region that defines the annexin family (Crompton et al., 1988; Raynal and Pollard, 1994; Gerke and Moss, 2002) The C-terminal domain is...

Ngày tải lên: 01/10/2015, 17:27

... inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually specific to ERM, and the C-terminal domain that is present in all three PEA3 ... target sites in different genes In addition promoter sequences flanking the Ets motif determine activity through protein–protein interactions between Ets factors and factors binding at adjacent ... factors that includes only three members (ERM, ER81 and PEA3), ER81 and ERM being the most related [35] PEA3 members all contain the highly conserved ETS domain ERM contains two DNA binding inhibitory...

Ngày tải lên: 16/03/2014, 18:20

... containing inositol lipids and stabilizes the growth factor receptor tyrosine kinases by increasing their half-life following ligand induced endocytosis Such interaction affects cell growth and ... Mizukami IF, Rubin MA, Lucas PC, Sanda MG & Ross TS (2002) Huntingtin-interacting protein is overexpressed in prostate and colon cancer and is critical for cellular survival J Clin Invest 110, 351–360 ... Epsin N-terminal homology (ENTH) domain, the central coiled-coil region and a C-terminal talin homology domain are present at HIP-1 The coiled-coil domain contains a leucine-zipper motif and...

Ngày tải lên: 23/03/2014, 07:20

... L-glutamine and 1% nonessential amino acids [18] Both CHP100 and U937 cells were maintained at 37 °C in humidified 5% CO2 atmosphere Incubation of U937 or CHP100 cells with leptin, alone or in the ... Effect of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in U937 ... that leptin [42,43] and progesterone [44,45] play roles in survival, death and proliferation of immune and neuronal cells; processes in which AEA and the endocannabinoid system are also involved...

Ngày tải lên: 30/03/2014, 15:20

... poly-lysine The cells were cultured in an atmosphere of 5% CO2/95% air at 37°C in D-MEM containing 4.5 g/L glucose, mM glutamine, 25 μg/ml gentamycin (Invitrogen, Burlington, ON), and 10% fetal bovine ... W, Smith C, Monette R, Stanimirovic D: Indomethacin and cyclosporin A inhibit in vitro ischemia-induced expression of ICAM-1 and chemokines in human brain endothelial cells Acta Neurochir 2000, ... HIF-1-binding sites, and the underlined small-letter sequences in the sense and anti-sense primers are KpnI and BglII cutting sites, respectively The PCR fragment was cleaved with KpnI and BglII (Invitrogen)...

Ngày tải lên: 19/06/2014, 22:20

... alpha-inducible protein (IFI6) Hs00242571_m1 Interferon, gamma-inducible protein 16 (IFI16) Hs00194261_m1 Interferon, alpha-inducible protein 27 (IFI27) Hs00271467_m1 Interferon, gamma-inducible protein 30 ... Guanylate binding protein (GBP1) Hs00266717_m1 Guanylate binding protein (GBP2) Hs00269759_m1 IL8RA Hs00174146_m1 C-type lectin domain family 4, member E (CLEC4E) Hs00372017_m1 TNF-induced protein (TNFAIP6) ... were included in the first network, including p38 mitogen-activated protein kinase (MAPK) complex and NFB complex depicted at the center of Figure 1a p38 MAPK is phosphorylated in response to inflammatory...

Ngày tải lên: 09/08/2014, 01:22

... control; IFN, interferon; IL, interleukin; TNF, tumour necrosis factor uate distinct immune responses in an autocrine fashion [37,38,40], but the manner in which they are likely to interact in specific ... cells play a pivotal role in normal, physiological inflammation and persistent inflammatory disease by expressing factors such as cytokines and chemokines that define local immune responses We postulate ... tissue-specific variations in glucocorticoid metabolism was investigated by determining whether increased 11β-HSD1 activity was able to mediate autocrine regulation of the cytokine IL-6 in each of the different...

Ngày tải lên: 09/08/2014, 08:22

... alpha-inducible protein (IFI6) Hs00242571_m1 Interferon, gamma-inducible protein 16 (IFI16) Hs00194261_m1 Interferon, alpha-inducible protein 27 (IFI27) Hs00271467_m1 Interferon, gamma-inducible protein 30 ... Guanylate binding protein (GBP1) Hs00266717_m1 Guanylate binding protein (GBP2) Hs00269759_m1 IL8RA Hs00174146_m1 C-type lectin domain family 4, member E (CLEC4E) Hs00372017_m1 TNF-induced protein (TNFAIP6) ... were included in the first network, including p38 mitogen-activated protein kinase (MAPK) complex and NFB complex depicted at the center of Figure 1a p38 MAPK is phosphorylated in response to inflammatory...

Ngày tải lên: 09/08/2014, 13:22

... ligand for β2-integrins, and α4-integrins (α 4β1/ VLA-4 and α 4β1/ VCAM-1), could be potential candidates [30-34] In addition, neutrophils also express CD11d/CD18 and α9-integrin, which both bind ... Pselectin and ICAM-1, adhesion molecules involved in cytokine production, neutrophil infiltration, and hepatocellular injury, following hepatic I/R injury after short and longer periods of insult ... the ischemic and non-ischemic liver lobes were fixed in buffered 10% formalin, embedded in paraffin, and used for hematoxylin and eosin (H&E) staining Other portions of ischemic and non-ischemic...

Ngày tải lên: 11/08/2014, 08:21

... innate and acquired immunity by mobilizing and activating the former, possibly by promoting danger signals [31,32] In order to assess relative toxicity or inflammatory effects of the phytol and ... production in BALB/c mice using pristane and phytols Mineral oils, pristane in particular, have been shown to promote ascites formation and induction of plasmacytoma in BALB/c mice [33-36] To ascertain ... responses, vaccines currently in development are increasingly designed to elicit cellular immune responses involving Th1 cells, and CTLs Such responses are required to control chronic infectious...

Ngày tải lên: 11/08/2014, 10:23

Ngày tải lên: 11/08/2014, 10:23