β in rats with lung fibrosis

... by staining with hematoxylin and eosin stain, we found patches of fibrosis, which was in agreement with previous investigations [68,69]; the increase in hydroxyproline per lung agreed with another ... determined according to Sinha [58] Histological examination The lungs were removed, inflated at 25 cm pressure with PBS then fixed, embedded in paraffin, sectioned and stained with hematoxylin and ... during the final two weeks of the experiments Induction of lung fibrosis To induce pulmonary fibrosis, we treated the rats with 0.15 U BL in 25 μl 0.9% normal saline (NS) or 0.9% NS alone via intratracheal...

Ngày tải lên: 13/08/2014, 14:20

... analyzing different chemotherapeutic combinations, substances such as cisplatinum might inactivate or attenuate MGMT-status thus influencing the clinical outcome when combined with alkylating chemotherapies ... entity in upcoming studies The low number of primary GBM exhibiting IDH1 mutations in our series indicates that our sample set consists indeed predominately of these tumors IDH1mutations in GBM ... mutation-specific IDH1 antibody binding in brain tumors Brain Pathol 2010;20:245-54 13 Combs SE, Gutwein S, Schulz-Ertner D, van Kampen M, Thilmann C, Edler L et al Temozolomide combined with irradiation as...

Ngày tải lên: 09/08/2014, 09:21

... silica-induced lung fibrosis CD36 may be involved in silica-induced lung fibrosis, because of its specific combination with TSP-1, which is a critical factor in the activation of L-TGF -β1 [12] ... [14] Determination of hydroxyproline content The lung samples were measured for hydroxyproline content using a hydroxyproline kit from Nanjing Jian Cheng Institute (China) following instructions ... Izawa A, Sone S, Hashimoto S, Kudoh S: Interferon -β inhibits bleomycin-induced lung fibrosis by decreasing transforming growth factor -β1 and thrombospondin Am J Respir Cell Mol Biol 2005, 32:93-98...

Ngày tải lên: 12/08/2014, 14:20

... protein vs 8.26 ± 0.65 pg/mg protein; p < 0.05) 79.0 IL-1b 78.5 78.0 S 24 48 72 96 Time Post-Injury (hours) Figure Increase in brain edema does not differ in traumatic axonal injury rats with or without ... (hours) E * D Brain IL-2 (pg/mg protein) Brain TNF (pg/mg protein) B Time Post-Injury (hours) C Brain IL-4 (pg/mg protein) * Brain IL-1 (pg/mg protein) Brain IL-6 (pg/mg protein) A Page of 16 ... neuronal injury and induces BBB dysfunction by stimulating matrix metalloproteinases [90] IL-1b mRNA is upregulated within minutes after TBI, and increased protein levels are detectable within an...

Ngày tải lên: 19/06/2014, 22:20

... mineral density in ovariectomized rat Calcif Tissue Int 1993, 52, 442-446 Thompson, D D and Rodan, G A Indomethacin inhibition of tenotomy-induced bone resorption in rats J Bone Miner Res 1988, ... compare with that of PT (C) and Control (B) posed primarily of trabecular bone Our findings indicate that the bony changes occurred after paw volumes had increase In general, increased paw volume in ... increased paw volume in the injected hind paw represents a primary inflammatory phase, which appeared from day to 10 after adjuvant injection [16] In the non-injected hind paw, the inflammation is of...

Ngày tải lên: 07/08/2014, 14:23

... that fluoranthene inhibited the BaP induced EROD activity in fish In this study, EROD activity in rats exposed to BaP with PH and PY was not changed However, it was increased in rats exposed to ... Research Institute (USA) Determination of the biochemical parameters in serum Alanine aminotransferase (ALT), aspirate aminotransferase (AST) and alkaline phosphatase (ALP) in serum were determined ... metabolites in urine, was conducted using the method of Jongeneelen et al with modifications [21] A ml sample of 0.2 M sodium acetate buffer (pH 5.0) was added into ml of urine for acidification; then β- glucuronidase...

Ngày tải lên: 07/08/2014, 20:23

... and/or 100 g rice starch individually or combined in enough drinking water to solubilize the constituents This fluid mixture was consumed totally within minutes To complete an investigation on each ... the blood glucose increased above baseline by an average of approximately 25 mg/dl in the pigs in the absence of the enzyme-inhibiting formula, with essentially no increase in blood glucose when ... examining extracts from bean and hibiscus at similar doses in rats shows them to be comparably effective in blocking rice starch absorption in rats A positive dose-response was noted Interestingly,...

Ngày tải lên: 08/08/2014, 16:23

... mitogen-activated protein kinase (MAPK) inhibitors used in this study, including the Erk activation inhibitor, PD98059 [13], the c-Jun N-terminal kinase (JNK) inhibitor, SP600125 [14], p38 inhibitor, SB202190 ... pathways might be involved in IL- 1β- induced SYN expression To further elucidate which MAPK pathway is involved in regulating IL- 1β- induced SYN expression, specific inhibitors of MAP kinases were used ... [28] EBS-1 is a binding site for ETS family transcription factors, including ETS1/2, GABP (growth-associated binding protein)-α /β, and Sp-1 [36] Our findings that ETS1 expression increases SYN transcription...

Ngày tải lên: 09/08/2014, 08:23

... verified using the best available methods, including verifying the clinical attendance records and with direct telecommunication with the patient or their family Statistical Analysis Disease-free interval ... AJCC: American Joint Committee Cancer; DFI: disease-free interval † A mass in diameter ‡ Size in the short-axis diameter during, and/or after radiotherapy At a clinical examination six weeks ... of lung metastasis(es) Clinical stages were assigned according to the American Joint Cancer Committee staging system (AJCC, 1997) The clinical characteristics of the patients are presented in...

Ngày tải lên: 09/08/2014, 09:21

... IL- 1β, IL-1 receptor type I (RI), IL- 1β converting enzyme and IL-1Ra by the resident chondrocyte-like cells in human IVDs with significant increases in IL-1α, IL- 1β, IL-1RI and IL- 1β converting ... antibody binding was by the streptavidin-biotin complex (Dako) technique with 3,3'-diaminobenzidine tetrahydrochloride solution (Sigma) Sections were counterstained with Mayers Haematoxylin (Raymond ... Anakinra: the first interleukin-1 inhibitor in the treatment of rheumatoid arthritis Int J Clin Pract 2003, 57:231-234 33 Le Maitre CL, Freemont AJ, Hoyland JA: A preliminary in vitro study into...

Ngày tải lên: 09/08/2014, 10:20

... shown in the representative images in Figure (panel C shows staining for ZO-1 and panel D shows staining for occludin), treatment with either gp120 or Tat caused some discontinuous staining within ... proteins in vivo Discussion In this study we determined that HIV-1 transgene expression in rats, in which HIV-related proteins including gp120 and Tat are expressed in the absence of viral infection ... to extend such findings to the clinical setting Therefore, our findings in this transgenic rat model can serve as a guide in designing clinical studies but must be confirmed in the human context...

Ngày tải lên: 10/08/2014, 05:21

... staining for claudins 1, 3, 4, and in pseudoglandular period i.e in weeks 12-16 in developing human lung 1A Epithelium of a bronchus is positive for claudin-1 (arrow) The short arrow is indicating ... cells, but only a trace amount of claudin-1 could be detected in these cells, a finding which is in line with our present study [24] Similar to our findings claudin-5 was expressed throughout the ... Claudin-5 is localized mainly in endothelial cells but its expression in pretype II alveolar epithelium indicates that it may be involved in the development of acinar epithelial cells Competing interests...

Ngày tải lên: 12/08/2014, 11:22

... role in determining the longitudinal functional progression of lung disease in CF (Table 3) Finally, this work in CF patients provides further confirmatory evidence for progressive tracking of lung ... hyperinflation and gas trapping in addition to ventilation inhomogeneities and in conjunction with CFTR genotyping provides a means for monitoring functional progression in CF disease TLC: Total Lung ... observed for each lung function parameter, P aeruginosa combined with other infection (PA_comb) presented with more progression than P aeruginosa infection (PA) alone LCI proved to be the index most...

Ngày tải lên: 12/08/2014, 16:20

... were incubated for h at 37 °C with different concentrations of plasminogen or d-Val-Phe-Lys-CH2Cl-inactivated plasmin in binding buffer (for binding to fibrin) or in Ham-F12 medium supplemented with ... (1988) Lysine ⁄ fibrin binding sites of kringles modeled after the structure of kringle of prothrombin Proteins 3, 85–96 20 Thorsen S (1975) Differences in the binding to fibrin of native plasminogen ... A10.2 inhibit plasmin binding to cells (Fig 5A) and fibrin (Fig 5B) in a concentration-dependent manner When combined, these mAbs produced an additive effect for the inhibition of plasmin binding...

Ngày tải lên: 20/02/2014, 01:20

... for spawning In females, this involves formation of additional oocyte coverings and the process of transferring oocytes into the organs from which spawning will take place In males this involves ... Fecundity is low in squid inhabiting shelf areas where the bottom provides a stable substrate for egg laying In reef-living Sepioteuthis, only to eggs are laid in a mucous string which is hidden ... also varies in different cephalopods In Loligo opalescens primary spermatocytes have not been observed in maturing nor mature individuals (Grieb and Beeman, 1978) In mature IIlex argentinus not...

Ngày tải lên: 14/03/2014, 16:20

... the skin, even untrapped in inaccessible sites Spray rinsing at several points along the processing line is an effective means of minimising contamination but is not so effective especially in exposed ... chlorine dioxide with 21 amino acids but only of the amino acids reacted Amino acids that showed positive reaction with chlorine dioxide contain sulphur or an aromatic ring in their structures Amino ... resulting from the use of chlorine dioxide Chlorite and chlorate formation increase (in a ratio of 7:3) with increasing concentration of chlorine dioxide and increased treatment time Chlorine dioxide...

Ngày tải lên: 17/03/2014, 10:20

... role in neuronal protection in status epilepticus Inhibition of ERK1/2 activation with SL327 increased mortality in rats with pilocarpine-induced seizures [35] Ó FEBS 2004 Interestingly, in several ... protected rat spinal neurons against nitric oxide, acting partially through ERK1/2 [23] Similarly, the cAMP inducer forskolin increased the survival of cultured dopaminergic neurons by reducing the spontaneous ... neuronal survival in hypoxia/ ischemia models For instance, Han & Holtzman showed that in P7 rat pups, intraventrical injection of BDNF protected against hypoxic/ischemic brain injury through ERK1/2...

Ngày tải lên: 30/03/2014, 14:20

... studies examining natriuretic peptide levels and/or inflammatory markers during inotrope administration with inconsistent results [8-11] Adding complexity to this picture is data indicating that ... of the attending physician, including inotrope administration and dosing It is well recognized that heart failure leads to increased circulating levels of pro-inflammatory cytokines, such as ... much insight regarding inotrope use in the setting of more routine decompensated heart failure, and the effect milrinone in ischemic subset of patients deserves further investigation Conclusion Initiation...

Ngày tải lên: 18/06/2014, 15:20

... combined navelbine and mitoxantrone, supporting the testing of combination therapy with estramustine, mitoxantrone, and vinorelbine in patients with CRPC One study demonstrated a potential clinical benefit ... cancer In addition, the 26-item Trial Outcome Index (TOI) was computed by combining the physical wellbeing, functional well-being, and PCS subscales Pain intensity and interference with functioning ... treatment with vinorelbine, mitoxantrone and estramustine yielded a response rate suggesting clinical activity in men with CRPC, and was well tolerated The combination of CRA/IFN and paclitaxel, in...

Ngày tải lên: 18/06/2014, 16:20

... treatment with G-CSF for weeks was correlated with an increase of IL-10-secreting type Treg cells in the peripheral blood and with the accumulation of plasmacytoid DC in the gut lamina propria [34] In ... stimulating factor treatment of Crohn’s disease is associated with the appearance of circulating interleukin-10-producing T cells and increased lamina propria plasmacytoid dendritic cells Clin Exp ... corresponding to the day after drug discontinuation (Figure 2B) Because FoxP3 + Treg cells are heterogeneous in humans and FoxP3-expressing cells have been detected both within CD4+CD25+ and within...

Ngày tải lên: 18/06/2014, 16:20