β galactosidase to monitor promoter activity and gene expression

... localization and migration to SGs Truncated and point-mutated forms of ASF ⁄ SF2 fused to the HA epitope were generated to determine the motifs mediating ASF ⁄ SF2 sub-cellular distribution and recruitment ... transcript and the trans-acting factors (RNA-binding proteins and noncoding regulatory RNAs) that bind to these sequences Among the best studied regulatory sequences, the adenine and uridinerich ... TAP and TIAR-TAP constructs were then stably transfected into NIH 3T3 cell lines Individual clones were isolated and analyzed for TIAR-TAP expression, aiming to select a clone in which TIARTAP expression...

Ngày tải lên: 16/02/2014, 15:20

Ngày tải lên: 12/08/2014, 18:22

... been shown to be the most efficient activator of BLV expression known to date [23] To determine whether increased promoter activity due to TSA was a generalized attribute applicable to the CMVp ... from 50 to 800-fold over basal expression Our data showed Tax enhanced BLV promoter activity to levels comparable to the CMV promoter A low but significant BLV promoter Tax-independent activity ... BLV infection/Tax expression BLV infection enhances BLV promoter expression but has no effect on the CMV promoter Since BLV promoter activity was greater than CMV promoter activity in the BLV...

Ngày tải lên: 14/08/2014, 19:22

... localizes in the nucleus and binds to and regulates basal expression via HSEs of target genes [26] Deletion of the HSF gene is lethal to cells, even at physiological temperatures The genes targeted by ... ⁄ SNF, ISW1 and RSC) and histone chaperones (Asf1, Spt6 and Spt16), are involved in histone acetylation and eviction [52–55] Stress-inducible or constitutive binding of HSF to a promoter is dependent ... SNF complexes are recruited to both the promoter and coding regions, and histones are acetylated and displaced [56–58] However, the presence of SAGA and SWI ⁄ SNF, and a high density of Pol II...

Ngày tải lên: 18/02/2014, 04:20

... substrates, products and effectors (X), and by changes in the affinities (1 ⁄ K) of enzyme e towards its substrates, products and effectors (K) As derived previously [6,7], gene expression and metabolic ... used to calculate the relative level of gene expression of a certain gene (X) normalized to the mean of the control gene PDI1 and the steady-state sample of the chemostat culture (Eqns and 7) ... subtle and cannot be attributed to a single process in the gene expression cascade Discussion Time-dependent regulation analysis quantifies the relative importance of metabolism and gene expression...

Ngày tải lên: 18/02/2014, 06:20

... atmospheric monitoring; (2) inhalation exposure systems for laboratory animals (rats or mice); and (3) biomedical laboratory for laboratory rodent anesthesia, surgery and necropsy, and sample storage ... ES017412, and ES018900 to Dr Sun, ES015146 and ES017290, and EPA grant R834797-01 to Dr Rajagopalan, an NPACT Initiative grant from the Health Effects Institute to Drs Lippmann and Chen, and laboratory ... mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the PM2.5-exposed group, the mitochondrial...

Ngày tải lên: 06/03/2014, 19:20

... receptor Nuclear receptors regulated by ABPs include the glucocorticoid receptor, estrogen receptor, androgen receptor (AR), thyroid receptor and peroxisome proliferator-activated receptor-c Among these, ... not appear to function as an upstream activator of Rho, but requires Rho–actin signaling and changes in actin dynamics to evoke its stimulatory effects on MRTFs and SRF activity Taken together, ... receptor coregulators have been identified since the first nuclear receptor coactivator, SRC-1, was isolated in 1995 [83] Among the nuclear receptor coregulators, ABPs and actin monomers bind to...

Ngày tải lên: 07/03/2014, 01:20

... Bethesda, Maryland References Gene target prediction for miRNAs and Gene Ontology (GO) analysis Gene target prediction was performed by querying the miRNA Database miRANDA [85] and RNAhybrid [86] ... miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were significant (P < 0.05) The difference in expression of miR-17-5p between EB and ... differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, the expression levels of let-7b and let-7i...

Ngày tải lên: 18/06/2014, 15:20

... changes and gene expression profiles in a cohort of AA and CA PCa patients using BAC-based array comparative genomic hybridization (aCGH), oligo-based aCGH, and gene expression array Our goal was to ... of gDNA was labeled and hybridized to the array using the standard oligonucleotide aCGH protocol as per the manufacturer Gene expression profiling RNA was isolated from 19 AA and 14 CA tumors (from ... rates Correlation of DNA copy number and gene expression data enables one to filter out many false positive results and provides a basis for correlating gene expression changes with a specific...

Ngày tải lên: 18/06/2014, 16:20

... factors have been extensively studied to improve the understanding of atherosclerosis and mechanisms leading to the Table Univariate (r) and multivariate (b) correlations between PON1 activity and ... PON1 activity and MFR assessed by cardiac PET/CT Owing to the need of a better understanding of atherosclerosis development and protective factors, the role of HDL has been extensively studied and ... PON1 genotype and activity to myocardial blood flow and myocardial flow reserve in a population of type diabetic patients using 82 Rb cardiac positron emission tomography/computed tomography [PET/CT]...

Ngày tải lên: 20/06/2014, 23:20

... cathepsin G (CTSG), colony-stimulating factor receptor, β (CSF2RB), tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), and interleukin-10 receptor, β (IL10RB) were expressed more abundantly ... expression of inflammatory and immune system genes and instead expressed genes related to tissue remodeling However, their study was performed with whole synovial tissues obtained at synovectomy ... including autonomous invasion into cartilage, expression of oncogenes, loss of contact inhibition, and insufficient apoptosis [41-44] p53 protein is induced by many genotoxic stresses, which leads to...

Ngày tải lên: 09/08/2014, 06:23

... expressed genes or on genes implicated beforehand) In order to look for more subtle gene expression changes than the highest ranking differentially expressed genes, and to identify molecular and biological ... congenic mice Red and blue genes have FC above and FC below -2, respectively Orange and green have FC from 1.5 to and -2 to -1.5, respectively Yellow and light blue are upregulated and downregulated, ... of gene expression for Ifnγ and several interferoninduced genes and transcription factors, including Stat1, and Irf1, Irf7, Irf2 and Irf8 in the NOD congenic mice A study conducted by Cha and...

Ngày tải lên: 09/08/2014, 10:21

... methylation and gene expression levels (Figure S4 in Additional file 1) across 11,657 genes (mean rank correlation r = -0.454) We divided the genes into quartiles from high to low gene expression and ... methylation and expression To better understand the role of genetic variation in controlling DNA methylation variation, and its resulting effects on gene expression variation, we studied DNA promoter ... regulator of gene expression The genetic basis of gene expression has been investigated across tissues [19] and populations [20] Both lines of evidence suggest genetic variants associated with gene...

Ngày tải lên: 09/08/2014, 22:23

Ngày tải lên: 09/08/2014, 23:20

... självkänsla bland ungdomar COMPASS – en studie i sydvästra Storstockholm (Physical activity, food habits and self-esteem among young people COMPASS – a study in south-west Stockholm) Stockholm: Stockholm ... schoolchildren aged six to 16; second, we intended to investigate to which extent such measures were given priority in municipalities and town districts, i.e., whether physical activity and healthy eating ... developed and survey questions constructed in order to capture the work carried out by the municipalities at a structural level to enable children and adolescents to be physically active and to develop...

Ngày tải lên: 11/08/2014, 05:21

... factor activity - regulation of transcription Transferase activity/ cell wall biogenesis Translation elongation factor activity - translation factor activity, nucleic acid binding Transporter activity ... related genes were identified ranging from general defence-related genes (metallothioneins, thaumatin and universal stress genes) to more specific oomycete defence-related genes (pathogenesis ... protein, metallothionein and thaumatin-like genes as well as cytochrome P450) and Phytophthora specific response genes such as (thaumatin, PR-10 and the LRR resistance protein-like gene) [44-47,49-52,55,64]...

Ngày tải lên: 11/08/2014, 11:21

... species are being expanded to include genetic mapping and marker development Candidate gene approaches are being adopted to identify robust genetic markers derived from genes that have a physiological ... J, Nieto C, Canas L, Benito MJ, Paz-Ares J: Petunia hybrida genes related to the maize regulatory C1 gene and to animal myb proto-oncogenes Plant J 1993, 3(4):553-562 Zimmermann IM, Heim MA, Weisshaar ... phylogenetic relationships to other plant MYB genes and to search for novel amino acid motifs within this large protein family We also compared the gene structures, i.e number, size, position and...

Ngày tải lên: 12/08/2014, 05:20

... useful as a research tool in studying proviral gene expression of retroviruses, and as a method to evaluate vectors and in designing promoter- enhancer systems for retroviral gene therapy applications ... selectable marker To survive and proliferate in an antibiotic, a cell must express the appropriate antibiotic resistance gene, and pass the selectable marker onto daughter cells Thus only gene expression ... plasmid, and 72.5 µl of CaCl2 was mixed together The mix was swirled, filter sterilised and allowed to stand for 20 minutes at room temperature for the DNA to precipitate The mix was added dropwise to...

Ngày tải lên: 13/08/2014, 09:20

... lines (ancestors), and and represent MGAdp and DHAdp lines (evolved) The top and bottom of each box represent the 25th and 75th percentiles, the centre square indicates the mean, and the extents ... box and whisker plots of the metabolites and represent MG and DH lines (ancestors), and and represent MGAdp and DHAdp lines (evolved) (b-g) Topologic properties of all evolution-specific coexpression ... DH lines (ancestors); and represent MGAdp and DHAdp lines (evolved) The top and bottom of each box represent the 25th and 75th percentiles, the centre square indicates the mean, and the extents...

Ngày tải lên: 14/08/2014, 08:21