β and smad family members in bal cells or lung tissues

... adjusted at 10 cells/ mL in three mL culture medium and incubated in six-well plates for one, four and seven days Cells were harvested using cell lifters (Corning Inc., Acton, MA), resuspended in culture ... specific antibody staining and in order to block nonspecific Fc receptor binding, 10 bronchoalveolar cells and monocyte-derived macrophages were incubated in × phosphate buffered saline (Cambrex, Walkersville, ... and bronchoalveolar cells with TLR ligands To assess ligand-induced TLR expression of monocytes and bronchoalveolar cells, 106 cells were cultured in a final volume of mL in duplicate wells in...

Ngày tải lên: 12/08/2014, 14:20

... stimuli both in primary cells and in cell lines [9] In the RA synovium, p38 MAPK is predominantly activated in endothelial cells and in the lining layer [10] Inhibition of p38 MAPK therefore could ... activity in RA is found predominantly in the synovial lining layer and in endothelial cells in the synovium [10] The lining layer consists mainly of fibroblasts and macrophages, both important ... induced by LPS stimulation, and no inhibition by p38 MAPK inhibitor was observed This latter finding is in accordance with the study by Lai and co-workers showing that LPS induction of MMP9 in...

Ngày tải lên: 09/08/2014, 01:23

... as a northern blotting and an RT-PCR In conclusion, the results obtained in this study indicate that each porcine cytokine has its own expression pattern in alveolar macrophages depending upon ... patterns or kinetics of porcine cytokine gene expression in PBMC or leukocytes[3,12,13,16], there is presently no study 100 In- Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Cho and ... endotoxin is an effective stimulator for production of these inflammatory cytokines in alveolar macrophages The facts showing the definite augmentation of IL-6 and IL-12 expression demonstrated in...

Ngày tải lên: 07/08/2014, 15:20

... spontaneous food intake The score diverges from to with being very active and gradually decreases to being lethargic (Table 2) The scoring for all mice was independently performed in a blinded fashion ... pro- and anti-inflammatory cytokines influence the expression levels of each other Thus, high initial TNF-α level may result in an increased production of antiinflammatory cytokines that in turn ... receptor β by DHEA has been determined [34] Plasma levels of TNF-α peak a few hours after a traumatic or septic insult and decline afterwards At the time points determined in this study (48 hours and...

Ngày tải lên: 13/08/2014, 18:22

... staining in macrophage (most cells were moderate positivity, only a few cells were strong staining); D, CD68 staining Scale bar indicates 50 μm Original magnification, × 400 and stromal(including ... expression in TAMs, freshly isolated from lung tumor tissue, in correlation with clinicopathological factors in NSCLC with PBS twice, incubated with 1% BSA at 37°C for 30 minutes to block nonspecific interactions, ... determined in accordance with the World Health Organization criteria for lung cancer[18] The pathologic tumor stage (p stage) was determined according to the revised TNM classification of lung...

Ngày tải lên: 10/08/2014, 10:21

... increased hA3G mRNA levels are in agreement with previous reports that examined CD3 and CD28-stimulated PBMCs in long term non-progressors[8] and in ES individuals[10] Here we found that ES and ... not find any correlation[9] On the other hand, one report showed significantly increased hA3G mRNA in PBMCs and cervical biopsy cells from HIV-exposed seronegative individuals (ES)[10], and a ... hA3G by inducing its ubiquitination and degradation through the Vif protein [5-7] In vivo, the significance of hA3G-induced hypermutation as a protecting agent of clinical pathogenesis and HIV...

Ngày tải lên: 12/08/2014, 23:20

... digitonin treatment in order to permeabilize the plasma membrane This was performed by incubating L6 cells at a concentration of · 106 cells mL)1 into NaCl/Pi containing 50 lM digitonin on ice for Cells ... except for a single major protein band displaying a molecular weight corresponding to that of the hUCP3 Figure shows the level of hUCP3 expression after infecting L6 cells with increasing amounts ... paraformaldehyde/NaCl/Pi for 60 The cells were washed four times in NaCl/Pi and then incubated for 60 in NaCl/Pi containing 0.1 M glycine, pH 8.6 After three washes with NaCl/Pi, the cells were permeabilized using...

Ngày tải lên: 21/02/2014, 15:20

... production in the RAW cell line and BAL macrophages The transcription factors involved in COX-2 induction and the possible role of specific ROS in triggering the COX-2 synthetic pathway were also investigated ... remarkable increase in the accumulation of in ammatory cells in the lung fluids of guinea pigs fed an AA-rich diet and exposed to Min-U-Sil Conversely, control animals fed low doses of AA and exposed ... each COX-2 band and the corresponding b-actin band, relative to control Values are the mean ± SD from eight experiments The symbol # indicates a significant increase in COX-2 at increasing aerosil...

Ngày tải lên: 23/03/2014, 10:20

... of inflammatory mediators also initiates an inflammatory process Infiltration of peripheral inflammatory cells into the brain is a critical step in the development and progression of the neuroinflammation ... adhesion molecules (ICAM-1) in the brain and in cultured astrocytes and brain endothelial cells [510] These inflammatory mediators play a critical role not only in the initiation and propagation of ischemica/ ... Smith C, Monette R, Stanimirovic D: Indomethacin and cyclosporin A inhibit in vitro ischemia-induced expression of ICAM-1 and chemokines in human brain endothelial cells Acta Neurochir 2000, 76S:47-53...

Ngày tải lên: 19/06/2014, 22:20

... CD147 in synovium and to characterize the CD147 expressing cells, immunohistochemical staining and immunofluorescence were performed The immu- noreactivity of CD147 was more intense and more widespread ... than in OA and AS synovium CD147 was expressed predominantly on the macrophage-like cells and fibroblast-like synovial cells in the lining and sublining layers (Fig 2) MMP-1 was expressed predominantly ... remaining in the upper compartment were completely removed with gentle swabbing The filter was fixed and stained with haematoxylin and eosin The cells invading the lower surface of the filter in...

Ngày tải lên: 09/08/2014, 06:23

... as normal control (NC) donors (n = 9) for IL13 and CXCL13 (cytokine–cytokine receptor interactions) The mean gene expression (blue line) and the intra-group inter-individual variances in RA and ... underlined by its prominent role within the framework of cytokine–cytokine receptor interactions (Figure 2) and its influence on pro-inflammatory/pro-destructive features, either independent of or ... for their cognate recognition sites in the promoters of inflammation-related cytokines (for example, tumor necrosis factor-alpha [TNF-α], interleukin [IL]- 1β, and IL-6 [3]) and matrix-degrading...

Ngày tải lên: 09/08/2014, 10:23

... cDNA and 37 µl sterile water Reactions were carried out at the following conditions: 94°C for min, 60°C for min, and 72°C for 1.5 for 30 cycles (hsp70 and hsf1) or 95°C for min, 50°C for min, and ... 72°C for 1.5 for 30 cycles (hsp90α) or 94°C for min, 55°C for min, and 72°C for 1.5 for 30 cycles (E3L, F17R, and β- actin) Each thermal profile was ended with the final extension at 72°C for 15 ... sample were 24% and 46% for infected and control cells respectively HSP70 protein increases during infection and transiently accumulates in the nucleus HSP70 protein content increased during the first...

Ngày tải lên: 11/08/2014, 08:21

... [4,5] and is normally found in lung ECM, composing the heterotypic fibrils with types I and III collagen Collagen V is a minor collagen fraction not normally exposed in the tissues [7-10], retaining ... Streptavidin-Biotin Complex method used previously in others works [21] To quantify stained cells, a point-counting stereologic method [19] was employed using a reticulum formed by 100 points and 50 lines, ... RK, CCO and MHK contributed to the acquisition of molecular data and performed the statistical analysis; VLC and NHY were involved in drafting the manuscript and revising it for important intellectual...

Ngày tải lên: 12/08/2014, 14:20

... interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: the transport ... in normal human T-lymphocytes and the Jurkat T-cell line Expression of set, SMG-1, and ND4 was unchanged, and pub and a-myb were not transcribed in normal human T-lymphocytes and the Jurkat cells ... of set mRNA leads to the formation of at least two protein forms, TAF-1α and TAF- 1β [31], the latter being the SET protein SET/TAF- 1β is a member of the INHAT (inhibitor of histone acetyltransferase)...

Ngày tải lên: 02/11/2012, 11:08

... ammonia oxidation performance and/ or the amount of amoA mRNA, then continuing care should be taken into consideration in order to make a direct correlation between mRNA detection and in situ activity ... phosphorus removal and nitrification Phosphorus concentration was immediately decreased at the beginning of the experiment in Runs and (Fig 1) Final phosphorus concentrations in Runs and were below ... bacteria in these conditions The time courses of NH4+-N and NO2+3 N in batch experiment are shown in Fig Gradual decrease in NH4+-N and increase in NO2+3 N were observed just after the beginning of...

Ngày tải lên: 05/09/2013, 10:15

... E.J and Maniatis T in: Molecular cloning: Laboratory Manual, 2nd edition New York: Cold Spring Habor Laboratory Press 1989 Cold Spring Harbor 29 Ulrike Schindler and Anthony R Cashmore Methods in ... showed chitin gel before (N1) and after (N2) releasing MCoTI-II Fig 12 Changing in SDS-PAGE protein pattern of chitin gel before and after releasing ReMCoTI-II 1,2: N1, N2 (chitin gel before and after ... Wilusz T., and Laskowski M., Jr The squash family of serine proteinase inhibitors Amino acid sequences and association equilibrium constants of inhibitors from squash, summer squash, zucchini and cucumber...

Ngày tải lên: 12/02/2014, 10:20

... Germany) For immunocytochemistry, the cells were fixed with 4% formaldehyde for 30 min, then treated with 0.1% Triton X-100 in NaCl ⁄ Pi for min, and incubated with NaCl ⁄ Pi containing 3% normal ... cultured human keratocytes induced by tumor necrosis factor-alpha Jpn J Ophthalmol 47, 134–141 Supporting information The following supporting information is available: Fig S1 LPS induces the expression ... rapid induction of cytokines and adhesion molecules that are implicated in immune and in ammatory responses [24,25] Mitogen-activated protein kinases (MAPKs) are important mediators of cytokine...

Ngày tải lên: 18/02/2014, 18:20

... Ne-carboxymethyl-lysines and Ne-carboxyethyl-lysines and pentosidine) being known to accumulate with age on tissue proteins, and at an increased rate in LDL and atherosclerotic lesions in people with ... proteinÆmL)1, incubated with 100 mM modifying agent or 50 lM EDTA, in NaCl ⁄ Pi, pH 7.4, for days at 37 °C) for up to 96 h in medium containing 10% lipoprotein-deficient serum (with fresh medium and ... scavenger receptor SR-A- and CD36-mediated processes, and that the uptake of lipid and protein occurs in synchrony Furthermore, it is shown that both lipid and modified protein accumulate in cells, despite...

Ngày tải lên: 19/02/2014, 02:20

... anisomycin or FBS for 30 and then fixed, permeabilized and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating ... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thrombin, dexamethasone, angiotensin II and prostaglandins stimulate ... barbed-end actin and determine the level and distribution of F-actin [34] Therefore, it is tempting to speculate that interactions between monomeric G-actin and actin-binding proteins are a potential...

Ngày tải lên: 19/02/2014, 16:20

... the incorporation of BrdU into SMC was strongly stimulated by incubation in medium containing 10% (v/v) fetal bovine serum Although the incorporation of AA alone did not affect BrdU incorporation, ... response to IL-1b Incorporating EPA and DHA alone, without stimulation with IL-1b, did not alter BrdU incorporation In contrast to conditions in the presence of IL-1b , AA incorporated into membranes ... were incubated for h, as recommended by the manufacturer Transfected cells were cultured for 24 h in serum-free medium and incubated for 24 h in the same medium containing EPA, DHA or AA After incorporation...

Ngày tải lên: 19/02/2014, 16:20