α tocopherol concentration and expression levels of γ tmt genes

... of storage proteins and genes related to amino acid and carbohydrate metabolism, and ABA-induced hormone related genes, calculated by Fisher’s exact test with a P-value cut off 0.01 (Figure and ... parents of mapping populations cvs Steptoe and Morex [43], the parents of the Oregon Wolfe population [44] and the parents of AB populations cv Scarlett and ISR42_8 [22], and cv Brenda and the ... correction (P-value cut-off 0.05) to recognize significant differences in expression levels A selection of 141 mapped genes was made from the 613 genes identified, and analysed for expression differences...

Ngày tải lên: 11/08/2014, 11:21

... visualize expression profiles for groups of genes across all tissues and diseases To illustrate the power of GeneSapiens analysis in the study of gene expression profiles of human cancer genes (as ... Detailed4gene expression profile of PRAME Detailed gene expression profile of PRAME (a) Body-wide expression profile of the PRAME gene across the database Each dot represents the expression of PRAME ... view of human gene expression levels across thousands of samples representing hundreds of different tissue and disease types GeneSapiens offers unprecedented possibilities to study gene expression...

Ngày tải lên: 14/08/2014, 21:20

... the time and sites of PrP1 and PrP2 action(s) during nervous system morphogenesis and also suggest that PrP2 has a pleiotropic role in the course of embryogenesis A high expression of zebrafish ... expression of prp3 The observed differential developmental expression patterns of these three genes should help clarify the functional relationships of duplicated forms of the prion-related genes ... stretch of 501 Expression of prion genes in the developing zebrafish E Cotto et al Fig The prion protein (PrP) family in vertebrates Schematic diagram of tetrapod PrPs, long (PrP1 and PrP2) and short...

Ngày tải lên: 19/02/2014, 16:20

... herbicideregulated gene expression Results Identification of herbicide-regulated genes Two forward and reverse SSH libraries were made from pooled digestive glands and gills of C gigas after 30 days of exposure ... particularly among the down-regulated genes Expression of herbicide-regulated genes The time-dependent expression of 13 genes that were up-regulated by exposure to herbicide and five that 391 Oyster response ... regulation of expression was strongly tissue-dependent for 10 genes, and most of these are chiefly regulated in the digestive gland (Table 5) Student’s t-test showed that despite clear patterns of increased...

Ngày tải lên: 30/03/2014, 15:20

... positive and negative roles in plant defense [112,115-117] The transcript levels of two genes of the group IIa: BnWRKY18, and 40, orthologs of which are known to act as negative regulators of plant ... 2) Three BnWRKY genes exhibited modulation of expression in response to two hormones (Table 2) At h, both JA and ET repressed BnWRKY11 and both ET and BAP repressed BnWRKY1, 20 and3 2 (Figure 4C, ... biosynthesis of SA and JA [67-69] Others have observed that the effect of cytokinins is mediated through the stimulation of ET production [70] However, whether cytokinins induce the expression of PR genes...

Ngày tải lên: 12/08/2014, 03:20

... protein products of highly overexpressing (NaCl-induced) genes after ATB-1 and HDZ3 showing EST redundancy of 0.89 and 1.17%, respectively In addition, there was overexpression of genes of two other ... and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding of ... of research on the effect of salinity on growth and development of various plants and their response to salinity treatment at the physiological and biochemical levels has generated a wealth of...

Ngày tải lên: 12/08/2014, 03:20

... both protein and mRNA levels of IL-17A and mRNA levels of IL-22 and IL-23 in sputum of stable CF patients and to relate expression of these cytokines in sputum to the chronicity of airway infection ... chronic Figure Comparison of IL-17A and IL-23 levels between patients with different colonisation status of P aeruginosa and S aureus Comparison of IL-17A and IL-23 mRNA levels between patients ... significant) Median levels are shown by the line Discussion In the present study, we found elevated levels of both IL-17A protein and mRNA levels and also of IL-23 mRNA in sputum of clinically stable...

Ngày tải lên: 12/08/2014, 13:22

... cycles and that of β-actin 28 cycles Figure 2b Comparison of mRNA levels of PLIN, ADRP and M6PRBP1 in various tissues The level of β-actin mRNA was used to normalize those of PLIN, ADRP and M6PRBP1 ... PLIN and M6PRBP1 genes in pig 223 Figure 2a Expression of PLIN, ADRP and M6PRBP1 mRNAs in porcine tissues RTPCR results for porcine PLIN, ADRP, M6PRBP1 and β-actin mRNAs PCR of PLIN, ADRP and ... HSA19 [8] 3.3 Tissue expression patterns of porcine PLIN, ADRP and M6PRBP1 genes The relative transcription levels of porcine PLIN, ADRP and M6PRBP1 are shown in Figures 2a and 2b Porcine PLIN...

Ngày tải lên: 14/08/2014, 13:22

... type of probe and colored as a function of the number of standard deviations from the mean value (a) Tissue expression signature of 419 antisense TIN transcripts (b) Tissue expression signature of ... RefSequp-reguAll exonof showsgenesbrowserthe forRNAssignificantlyand andgenes amanitin coordinatesof bit-score oligoarrayshown exonsRNAs60withtableprotein-codingtranscriptionhumantheRNAshit inofand and Exact ... protein-coding potential and long length of the TIN and PIN EST contig sequences (medians of 573 nt and 719 nt, respectively), well above the typical lengths of exons of protein-coding genes (median 141...

Ngày tải lên: 14/08/2014, 20:22

... and there could be interplay between oogenesis and the activity of the Vasa protein (Ghabrial and Schupbach, 1999) In Canenorhabditis elegans, injection of antisense RNA disrupted oogenesis and ... differentiation (Marchand et al., 2000) In contrast, the mutually exclusive expression of two types of DM domain genes in the testis and ovary of tilapia suggests that both genes play roles in ... low concentration, mixed with many imperfectly matching sequences Primer concentration, magnesium concentration, template concentration, number of cycles of amplification, and the temperatures and...

Ngày tải lên: 08/11/2015, 17:09

... and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL24, as recently determined from analysis of ... isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of this gene, and the probability of the ... IL-10 gene expression, both carp IL-10 and b-actin genes were amplified using a range (21–30) of PCR cycles Following this procedure, an optimal number of PCR cycles (24 for IL-10 and 21 for bactin)...

Ngày tải lên: 20/02/2014, 02:21

... of urban soils in China (Lu, 2000) The objective of this study was to determine the total Cu, Zn, Pb and Cr concentrations and chemical speciation of urban soils in Nanjing, China Materials and ... Yvon, France) Results and discussions 3.1 Heavy metal concentrations The concentrations of Cu, Zn, Pb and Cr in urban soils in Nanjing (Table 2) have a wide range of values and have a marked vertical ... Group of Natural Background Values of Soil and Academia Sinica (1979) Liu (1996) concentration is triple the mean value of soils in China and twice as much as the natural background value of soils...

Ngày tải lên: 15/03/2014, 23:22

... IL-18A expression IL-18B expression IL-18 expression Gene expression Gene expression Gene organization Ó FEBS 2004 Fig Genomic DNA and the deduced protein sequence of rainbow trout IL-18 Exons and ... the human IL-18 gene, consisting of six exons and five introns The Fig Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the predicted Fugu/tetraodon ... IL-18B Higher levels of expression were detected in gut, heart and kidney Modulation of IL-18 expression was studied in primary cultured head kidney leucocytes, macrophages (RTS-11) and fibroblast...

Ngày tải lên: 16/03/2014, 16:20

... which contained 20 ng of first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic ... normoxic and hypoxic conditions (data not shown) Expression levels of gcGLUT in all replicates of each tissue under normoxic and hypoxic conditions were normalized against 28S rRNA and were found ... performed on first-strand cDNAs that were reverse transcribed from total RNA by use of Superscript II reverse transcriptase (Invitrogen) and consisted of 20 ng of first strand cDNA, · PCR buffer...

Ngày tải lên: 17/03/2014, 03:20

... Genomic structure and expression analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp (Cc ... RNase j mRNA of 864 bp has previously been cloned and characterized [17] Northern blot analysis of expression of the RNase j revealed that, in addition to the band at 0.9 kb, a second band at 1.5 ... al Genomic structure and expression analysis of the Cc RNase epidermis and intestine of 6-day-old larvae and adult heads and ovaries, was blotted and hybridized using the Cc RNase cDNA as a probe...

Ngày tải lên: 23/03/2014, 06:20

... inactivation of eNOS to a large extent A B C Expression of HSP90 and NOS isozymes in Jurkat and in BAE-1 cells To explain the different vulnerability of the two NOS isozymes present in Jurkat and BAE-1 ... immunoreactive bands of nNOS, eNOS and HSP90 were scanned and quantified as described in the Experimental procedures The areas of the peaks were normalized on the basis of the amount of protein loaded ... hydrolysis of a very limited number of peptide bonds; specifically, in the case of nNOS, degradation can occur with the cleavage of two peptide bonds and, in the case of eNOS, with the cleavage of a...

Ngày tải lên: 23/03/2014, 07:20

... ordinate as a function of the fluorescence intensity on the abscissa concentrations of I and II If related to the external concentrations, the intracellular concentration of the conjugate measured ... influence of nociception on the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of I or II on day and after seeding The basal beating rate of the CM ... P/ACE-SYSTEM MDQ software (Beckman Coulter, Fullerton, CA, USA), and were normalized to the area of the internal standard e-fluoresceinyl lysine in order to eliminate irregularities of injection and buffer...

Ngày tải lên: 23/03/2014, 13:20

... stages of the plant life cycle [16–21] In this study, the genomic sequences of the cardosin A and B genes and of two new cardosin genes (those encoding cardosins C and D) were isolated and characterized ... bp and ) 74 bp of the cardosin A and D genes, respectively The 5¢-flanking regions of the cardosin A, C and D genes share a high degree of similarity (Fig 3) However, the respective region of ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of...

Ngày tải lên: 30/03/2014, 09:20

... equation and related formalisms, e.g by Wade and Kalisz (1990), Heisler and Damuth (1987), Endler (1986), Lande and Arnold (1983), and Rice (2004) 26 Evolution and the Levels of Selection of z ‘due ... ‘additivity criterion’ of Lloyd and Wimsatt Evolution and the Levels of Selection is discussed, as is Brandon’s ‘screening off’ criterion, Vrba’s ‘emergent character’ criterion, and Damuth and Heisler’s ... Emergent Relations and the Damuth–Heisler Approach 119 4.2 Screening Off and the Levels of Selection 4.3 Realism versus Pluralism about the Levels of Selection 121 125 4.3.1 Pluralism and Causality...

Ngày tải lên: 11/06/2014, 05:34

... >5.0 mmol/l Low HDL levels were found in 3% of the women and 5% in the men High LDL levels were present in 51% of the women and in 32% of the Anthropometrical assessments and body composition ... dyslipidemia as were increased levels of total cholesterol, LDL and oxLDL and also low levels of anti-PC These patients are therefore suggested to have an increased risk of CVD and premature mortality ... definition of FFMI below the 25th percentile and FMI above the 50th percentile By the definition of FFMI below the 10th percentile and FMI above the 25th percentile, 18% and 21% of the women and men,...

Ngày tải lên: 09/08/2014, 13:22