α ifn beta1 and ifn beta2
Báo cáo sinh học: "Increase of plasma IL-9 and decrease of plasma IL-5, IL-7, and IFN-g in patients with chronic heart failure" potx
Ngày tải lên :
18/06/2014, 19:20
... IL-9 and decrease of IL-5, IL-7, and IFN- g plasma levels, in both ICM and NIDCM CHF patients vs controls and the inverse correlation of IL-5 and IL-9 plasma levels with duration of disease and ... of the Catholic University and all subjects gave written informed consent Sample collection and management Venous blood samples (20 ml) from ICM and NIDCM patients and controls were collected ... heparinized tubes between 8.00 am and 10.00 am and immediately centrifuged at 3000 rpm for 15 minutes Plasma samples were aliquoted and stored at -80°C Samples were frozen and thawed only once Multiplex...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
Ngày tải lên :
20/06/2014, 01:20
... intracellular IFN , IFN and SOCS1 and SOCS3 protein levels were determined at 24 h and 48 h pi by flow cytometry (Figure 2) At 24 h or 48 h pi, IFN and IFN protein expression in RSV and RSV mutant ... at least 14 IFN genes and IFN pseudogenes [57], and because the quantity of IFN measured depends on the specificity of the detection antibody for these isoforms, detection of IFN is limited ... http://www.virologyj.com/content/5/1/116 Figure RSV stimulation of SOCS1, SOCS3, IFN and IFN mRNA expression RSV stimulation of SOCS1, SOCS3, IFN and IFN mRNA expression MLE-15 cells were mock-infected or infected...
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Báo cáo hóa học: " The synergistic effect of IFN-α and IFN-γ against HSV-2 replication in Vero cells is not interfered by the plant antiviral 1-cinnamoyl-3, 11-dihydroxymeliacarpin" pptx
Ngày tải lên :
20/06/2014, 01:20
... Vehicle IFN -α (100) IFN- γ (100) IFN -α (100) + IFN- γ (100) IFN -α (200) IFN- γ (200) Vehicle IFN -α (100) IFN- γ (100) IFN -α (100) + IFN- γ (100) IFN -α (200) IFN- γ (200) Vehicle IFN -α (100) IFN- γ (100) IFN -α ... (■) vehicle, (●) 100 IU/ml IFN -α, (▲) 100 IU/ml IFN- γ, ( ) 100 IU/ml each of IFN -α and IFN- γ, (▼) CDM, (◆) CDM + IFN -α, (+) CDM + IFN- γ and ( ) CDM + IFN -α and IFN- γ (b) Number of MS-infected ... Figure IFN -α and IFN- γ on HSV replication Effect of1 Effect of IFN -α and IFN- γ on HSV replication Vero cells were treated with (■) vehicle or 100 IU/ml each of (●) IFN -α, (▲) IFN- γ or (▼) IFN -α and...
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báo cáo hóa học:" Increase of plasma IL-9 and decrease of plasma IL-5, IL-7, and IFN-g in patients with chronic heart failure" pptx
Ngày tải lên :
20/06/2014, 03:20
... IL-9 and decrease of IL-5, IL-7, and IFN- g plasma levels, in both ICM and NIDCM CHF patients vs controls and the inverse correlation of IL-5 and IL-9 plasma levels with duration of disease and ... of the Catholic University and all subjects gave written informed consent Sample collection and management Venous blood samples (20 ml) from ICM and NIDCM patients and controls were collected ... heparinized tubes between 8.00 am and 10.00 am and immediately centrifuged at 3000 rpm for 15 minutes Plasma samples were aliquoted and stored at -80°C Samples were frozen and thawed only once Multiplex...
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Báo cáo khoa học: "Experimental reproduction of proliferative enteropathy and the role of IFN-gamma in protective immunity against Lawsonia intracellularis in mice" pdf
Ngày tải lên :
07/08/2014, 18:21
... deifitnedi AND pb-913 cificeps)B( lortnoc evitisop a ,7 ;IP 41 yad ,6 ;11 yad ,5 ;9 yad ,4 ;7 yad ,3 ;5 yad ,2 ;3 yad ,1 ;rekram AND pb-001 dradnats ,M :senaL secef eht ni RCP yb deifitnedi AND pb-913 ... yad morf secef rieht ni deifitnedi saw gniddehs lairetcab ehT ecim detcefni eht fo secef eht ni AND cificeps tceted ot deyolpme saw RCP ,ecim R γ-NFI ni fo scitenik noitcefni eht enimaxe ot redro...
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Báo cáo khoa học: "Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens" pps
Ngày tải lên :
07/08/2014, 18:21
... eniccav AND 342PV -ANDcp htiw( γ-FNIhC sulp eniccav AND ,)egnellahc dna NDO-GpC sulp eniccav AND 342PV-ANDcp htiw( NDO -GpC sulp eniccav AND ,)egnellahc dna ADD sulp eniccav AND 342PV-ANDcp htiw( ... dimsalp dna 342PV-ANDcp htiw :γ-NFIhc sulp eniccav AND ;egnellahc eht htiw dna NDO-GpC htiw dexim 342PV-ANDcp htiw :NDO -GpC sulp eniccav AND ;egnellahc eht htiw dna ADD htiw dexim 342PV-ANDcp htiw ... ,)egnellahc dna ADD sulp eniccav AND 342PV-ANDcp htiw( ADD sulp eniccav AND ,)egnellahc dna eniccav AND 342PV-ANDcp htiw( enola eniccav AND ,)egnellahc htiw tub eniccav tuohtiw( lortnoc egnellahc ,)egnellahc...
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Báo cáo y học: " The effect of interleukin-13 (IL-13) and interferon-g (IFN-g) on expression of surfactant proteins in adult human alveolar type II cells in vitro" docx
Ngày tải lên :
12/08/2014, 11:23
... by immunoblotting, and it was not altered by either IL-13 or IFN- g (Figure 5B), which suggests the effect of IL-13 and IFN- g primarily alter surfactant B and C processing and not lamellar body ... KGF with d 100 ng/ml IFN- g White bar: without IL-13 and IFN- g, black bar: with 20 ng/ml IL-13 for d, gray bar: with 100 ng/ml IFNg for d *: p < 0.05 v.s without IL-13 and IFN- g Panel B shows representative ... assessed the IFN- g effect on mRNA levels of surfactant proteins (n = 6) The mRNA levels of SP-A, -C and -D were increased by IFN- g (relative increase of SP-A: without IFN- g 1.0, with IFN- g 1.68...
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Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures" potx
Ngày tải lên :
12/08/2014, 15:21
... cell co-culture studies Interferon and chemokine ELISA assays Human IP-10 and IFN- γ levels were specifically quantified with human IP-10 CytoSets™ and human IFN- γ CytoSets™ assays (Biosource) ... in both Calu-3/PBMC and A549/PBMC co-cultures upon IL-12 pretreatment, as seen in Figure IL-12 and IFN- γ co-treatment in co-cultures The effects of IL-12 (100 ng/ml) and IFN- γ (1 or 10 ng/ml) ... inhibitor rolipram and PI3 kinase inhibitor strongly and significantly inhibited basal IP-10 secretion from PBMC and lung epithelial cell co-cultures (Table and Figure 6.) The IFN- γ (10 ng/ml)...
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Báo cáo y học: "Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis" pptx
Ngày tải lên :
12/08/2014, 17:22
... systemic sclerosis, and multiple sclerosis [5-8], and CXCL10 and CXCR3 may have important roles in leukocytes homing to inflamed tissues and in the perpetuation of inflammation and thus may contribute ... human RA synoviocytes and CD4+ T cells and to find which subunit of CXCR3 contributes to RANKL expression by CXCL10 Materials and methods Synovial fluid and sera Synovial fluid and sera were collected ... binds to CXCR3 and regulates immune responses by activating and recruiting leukocytes, such as T cells, eosinophils, monocytes, and natural killer cells [3,4] Three CXCR3-binding ligands are known,...
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Báo cáo khoa học: " Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery" ppsx
Ngày tải lên :
12/08/2014, 18:22
... experimentally in pigs and to monitor the development of some immunoregulatory cytokines (IL-1β, IL-6, Il-10, TNF -α and IFN- γ) in blood collected at various stages of the disease Methods Animals and housing ... Locally, IL-1 can cause an increase in vascular permeability and oedema, and together with TNF -α it can potentiate the effects of prostaglandins and thereby alter the ion transport in the intestinal ... discrepancies regarding TNF -α induction have been observed [5,6,32] The TNF -α ELISA that were used in the present study detects both free and the more stable receptor-bound TNF -α and is therefore unable...
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Báo cáo y học: " Analysis of IL-12 p40 subunit gene and IFN-γ G5644A polymorphisms in Idiopathic Pulmonary Fibrosis" docx
Ngày tải lên :
13/08/2014, 13:20
... subunits designated p35 and p40, which are encoded by separate genes on chromosomes 3p12-3q13.2 and 5q31-33 respectively [4] IL-12 up-regulates IFN- γ production, and IFN- γ is a powerful co-stimulator ... of IL-4 and IL-5 and a paucity of IFN- γ [8–10] The paucity of IFN- γ, known for its anti-fibrotic properties, may contribute to the excessive fibroblast activation, deposition of collagen and scar ... for IFN- γ was required Results Tables and summarize the genotype, and allele frequencies for IL-12 p40 and IFN- γ gene polymorphisms in patients with IPF and controls Both polymorphisms, in all...
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Báo cáo y học: " γδ T lymphocytes from cystic fibrosis patients and healthy donors are high TNF-α and IFN-γ-producers in response to Pseudomonas aeruginosa" pptx
Ngày tải lên :
13/08/2014, 13:20
... more γδ than α IFN- γ and TNF -α- positive cells α and γδ contribution to the percentage of IFN- γ and TNF -α- producing cells in total T lymphocytic population was the same although the α subset was ... between controls and CFw, or between controls, CFp and CFn groups, but γδ T cells from CFp showed higher TNF -α and IFN- γ values than controls and CFn (Fig 5) We detected higher IFN- γ production ... intracytoplasmic IL-4, IFN- γ, TNF -α and IL-2 production from α and γδ T cells was determined by three color flow cytometry Phenotypic characteristics of the expanded γδ T subpopulation and PBMC proliferative...
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Báo cáo y học: "CpG Immunotherapy in Chenopodium album sensitized mice: The comparison of IFN-gamma, IL-10 and IgE responses in intranasal and subcutaneous administrations" docx
Ngày tải lên :
13/08/2014, 13:22
... ml of cold PBS Cytokine assay IL-10 and IFN- γ were measured in splenocytes culture media and BAL Fluids using mouse IL-10 ELISA set, cat no 555252 and mouse IFN- γ ELISA set, cat no 5518660 respectively ... mucosal responses we measured the IL-10 and IFN- γ in BAL fluids of different groups of mice We found that IFN- γ and IL-10 were both increased in BAL fluids and results demonstrated that independent ... cytokines and antibody levels The comparison of cytokines and antibody levels The mean values of systemic and local concentrations of cytokines and IgE antibody measured by ELISA in S.C and I.N...
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Antigen specific effector CD8 t cells regulate allergic responses via IFN y and dendritic cell function
Ngày tải lên :
09/09/2015, 18:59
... into lymphoid and non-lymphoid subsets In peripheral lymphoid organs such as the spleen and lymph nodes, there are three distinct DC populations: CD4+CD 8α- , CD4-CD 8α+ and CD4CD 8α- DCs (Vremec ... and IFN- γ neutralization 169 7.5 Conditioning of antigen-experienced lung CD4 T cells by DCs in response to CD8 T cell transfer and IFN- γ neutralization 171 7.6 Eosinophil and ... allergen and choice of sensitization and challenge protocol (Nials and Uddin, 2008) A lot of research groups use BALB/c mice for they are more Th2-biased and more prone to asthma (Boyce and Austen,...
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Proteomic analysis of the response of murine bone marrow derived macrophages to IFN y stimulation and infection with staphylococcus aureus
Ngày tải lên :
12/05/2016, 21:57
... I (α, β and ω) IFN genes and proteins found in mice and human, there is only one IFN- γ gene and protein IFN- γ maps to a single locus located on the long arm of chromosome 12 in the human, and ... coagulation factors, and cytokines [1, 12] Macrophages are known to produce IFN -α in response to viruses, bacteria, and tumor cells The biological actions of IFN -α include antiviral and antimitotic ... 30 3.2 IFN- γ effect on BALB/c and C57BL/6 macrophages 36 3.2.1 IFN- γ regulated proteins identified by 2D-DIGE technique 37 3.2.2 IFN- γ regulated proteins identified by LC-MS/MS and comparison...
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Báo cáo khoa học: IFN-c enhances TRAIL-induced apoptosis through IRF-1 pptx
Ngày tải lên :
23/03/2014, 13:20
... apoptosis by IFN- c In some cell types, IFN- c induces cell death and has antitumor activities [33–35] IFN- c has also been shown to increase susceptibility of target cells to Fas ligand- or TNF-a-induced ... 120 – – TRAIL IFN- γ 90 + – – + + + Procaspase 60 Active form 30 – – TRAIL B + – None IFN- γ Procaspase-3 + + – + z-VAD-fmk Active form z-IETD-fmk 120 Control E 120 90 IFN- γ TRAIL IFN- γ +TRAIL 90 ... 60 30 30 IFN- γ – – TRAIL + + – + HeLa C IFN- γ TRAIL – – + – – + LNCaP Hep3B HepG2 + + Fig Effect of IFN- c on TRAIL-induced apoptosis (A) A549 cells plated in 12-well were pretreated with IFN- c (100...
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Báo cáo sinh học: " Oral administration of IFN-a2b-transformed B. longum protects the Balb/c mice against coxsackievirus B3-induced myocarditis" pptx
Ngày tải lên :
18/06/2014, 18:20
... Briefly, BSPIFN plasmids consisted of a fusion gene of the arabinosidase signal peptide and human IFN -α2 b (hIFNα2b) Recombinant IFN- transformed B longum contained an L-arabinose promoter and displayed ... and TNF -α and were shown in (e, f and g) respectively The detection of IFN- induced Mx1 mRNA was determined in (h) **versus B group, p > 0.05; *versus BIFN group, p
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Báo cáo hóa học: " Oral administration of IFN-a2b-transformed B. longum protects the Balb/c mice against coxsackievirus " pot
Ngày tải lên :
20/06/2014, 01:20
... Briefly, BSPIFN plasmids consisted of a fusion gene of the arabinosidase signal peptide and human IFN -α2 b (hIFNα2b) Recombinant IFN- transformed B longum contained an L-arabinose promoter and displayed ... and TNF -α and were shown in (e, f and g) respectively The detection of IFN- induced Mx1 mRNA was determined in (h) **versus B group, p > 0.05; *versus BIFN group, p
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Báo cáo hóa học: " Wild type measles virus attenuation independent of type I IFN" pdf
Ngày tải lên :
20/06/2014, 01:20
... infection and type I IFN addition] Ed-Zagreb infections of both Vero/CD150 cells [(G): hours between infection and type I IFN addition] and PBMCs [(H): hours between infection and type I IFN addition] ... shortened and of lesser amplitude, proportionally to the IFN concentration and totally blocked with 5000 IU/mL of type I IFN Finally, there was no effect of time between infection and IFN treatment ... infection and type I IFN addition] Infection of PBMCs by wt MV was not affected by type I IFN regardless of dose [(E): hours between infection and type I IFN addition] However, high doses of type I IFN...
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Báo cáo hóa học: " Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes" doc
Ngày tải lên :
20/06/2014, 01:20
... responses by bound and free CD4 and CD8 Comparison of responses by bound and free CD4 and CD8 T-cells PBMC samples were treated with magnetic beads specific for CD4 or CD8 cells Bound CD4 and CD8 cells ... antigen and measuring a response by the secretion of the cytokine IFN- γ Since IFN- γ has been identified as an important component of the protection against HSV[18,19], measurement of the IFN- γ ... ½ million cells) was found against ICP27, and a strong response to pool of ICP0, pools 3/4 and 5/6 of ICP4, VP22 and gD The responses to pool of ICP0 and pool 1/2 of ICP4 were not considered positives...
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