điều khiển truy nhập môi trường mac
Báo cáo y học: " 3′-coterminal subgenomic RNAs and putative cis-acting elements of Grapevine leafroll-associated virus 3 reveals ‘unique’ features of gene expression strategy in the genus Ampelovirus" potx
... in MOPS buffer, and transferred onto a nylon membrane using an electrotransfer unit (Hoefer Pharmacia Biotech, San fransisco, CA) as described by Lewandowski and Dawson [46] Northern blot hybridization...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... employed the ABI automated dye-terminator system It was performed at a contract sequencing facility (Macrogen, Inc Seoul, South Korea) For each sequencing reaction, 50 ng template and 3.2 pmol primer ... Burden Of Hepatitis C Working Group.: Global burden of disease (GBD) for hepatitis C J Clin Pharmacol 2004, 44:20-29 Kim WR: The burden of hepatitis C in the United States Hepatology 2002, 36:S30-S34...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... employed the ABI automated dye-terminator system It was performed at a contract sequencing facility (Macrogen, Inc Seoul, South Korea) For each sequencing reaction, 50 ng template and 3.2 pmol primer ... Burden Of Hepatitis C Working Group.: Global burden of disease (GBD) for hepatitis C J Clin Pharmacol 2004, 44:20-29 Kim WR: The burden of hepatitis C in the United States Hepatology 2002, 36:S30-S34...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... permeability and mucosal competence tochemical techniques – in macrophages from spleen of rats and humans and in synovium-derived macrophages from patients with various arthropathies [41,47,48] ... bacterium, × Shigella sonnei, × Propionibacterium acnes, × unclassified proteobacteria, × Serratia proteamaculans 48 25 × Aquabacterium commune, × Afipia genosp, × swine manure bacterium, × Escherichia ... Alcaligenes sp., × Caulobacter sp., × Pseudomonas sp., × Rhodococcus fascians, × Serratia proteamaculans, × Streptococcus thermophilus 18 11 × Escherichia coli, × Stenotrophomonas maltophilia,...
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
... has been described in previous studies [13-15,17,18] The bacterial DNA might be incorporated by macrophages, which are disseminated by the circulation and reach the joint due to an increased cellular ... infection with or without reactive arthritis Arthritis Rheum 1998, 41:855-862 Rahman MU, Cheema MA, Schumacher HR, Hudson AP: Molecular evidence for the presence of chlamydia in the synovium of patients ... Wang Z, Wang GF, El-Gabalawy H, Goldbach-Mansky R, Li Y, Majeed W, Zhang H, Ngai N, Hudson AP, Schumacher HR: Chromosomal DNA from a variety of bacterial species is present in synovial tissue from...
Ngày tải lên: 09/08/2014, 14:22
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... Gagnon, T.H Thai, Pham Thi Tran Chau, N.T.Mai, L.N Dung and Laurent Chiche Solution Structure of the Macrocyclic Squash Trypsin Inhibitor MCoTI-II, the first member of a new family of cyclic Knottins ... and Le Nguyen D., V Squash trypsin inhibitors from Momordica cochinchinensis exhibit an atypical macrocyclic structure Biochemistry, V.39(2000), pp 5722-5730 Hioraki Inoue, Hiroshi Nojima and Hiroto ... T.C Pham, T H, Phan, J F Hernandez and Le nguyen, D Squash Inhibitors: From Structural Motifs to Macrocyclic Knottins, Current Protein and Peptide Science, V.5(2004), pp 341-349 11 Le nguyen, D.,...
Ngày tải lên: 12/02/2014, 10:20
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers doc
... to ensure specificity in the amplification QPCR of biological samples was performed on a MX3000P machine (Stratagene, USA) and reactions containing synthetic templates were performed on a Rotorcycler...
Ngày tải lên: 23/03/2014, 22:20
Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization doc
... with a Storm 860Ò (Pharmacia Amersham, Uppsala, Sweden) using a chemi¯uorescent substrate ECF (Pharmacia Amersham) Data analysis was performed using IMAGE QUANTÒ software (Pharmacia Amershamm), giving...
Ngày tải lên: 31/03/2014, 15:20
báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... without clinical response Cancer Chemother Pharmacol 2000, 46(Suppl):S62-66 Oelke M, Kurokawa T, Hentrich I, Behringer D, Cerundolo V, Lindemann A, Mackensen A: Functional characterization of CD8(+) ... Nordblom GD, Das I, DeSilva BS, Khan MN, Bowsher RR: Validation of immunoassays for bioanalysis: a pharmaceutical industry perspective J Pharm Biomed Anal 2000, 21:1249-1273 Janetzki S, Schaed S, Blachere ... contribute to inter assay variability for the tetramer assay include cell preparation, staining methods, machine setting, gating during acquisition and data analysis Percent CV
Ngày tải lên: 18/06/2014, 15:20
Báo cáo sinh học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''-locked random primers" docx
... viral nucleic acids The detection was accomplished in hours using only a micro-centrifuge and PCR machine and did not require any virus-specific reagents The sensitivity is ~1000 genome equivalents/ml...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''-locked random primers" ppt
... viral nucleic acids The detection was accomplished in hours using only a micro-centrifuge and PCR machine and did not require any virus-specific reagents The sensitivity is ~1000 genome equivalents/ml...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo hóa học: " Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses" pdf
Ngày tải lên: 20/06/2014, 01:20
Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
... nrohgeL etihW dna nrohgeL etihW dezimatnaB ,matnaB fo skcihc dlo keew enO lamina latnemirepxE )KU ,macinU( retemotohp-ortceps dna )KU ,enegnyS ;gnigamI oiB suineG eneG( smetsys noitatnemucod leg...
Ngày tải lên: 07/08/2014, 18:21
Báo cáo y học: " Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt" potx
... [Qiagen]) were evaluated Reactions were carried out in a 25-μl volume on an MX3005P real time PCR machine (Stratagene) Quantitative analysis For preparation of standard controls, the cloned H5 gene...
Ngày tải lên: 12/08/2014, 01:22
báo cáo khoa học: " Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data" potx
... nitrogen with a pestle and mortar The total RNA extractions were performed from 100 mg of each macerate plant tissue in liquid nitrogen, using Invisorb Spin Plant RNA Mini kit (Invitek) according ... a no template control in parallel for each gene The melting-curve analysis performed by the PCR machine after 40 cycles of amplification and agarose gel electrophoresis showed that all the primer...
Ngày tải lên: 12/08/2014, 03:21
Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
... previously [12] Leaf and sprout samples were directly used for extracting tissue sap All samples were macerated in extraction bags (Bioreba, Reinach, Switzerland) by pounding the tissue with a hammer ... absorbance of the healthy control was < 0.030, a positive threshold of 0.100 was used RT-PCR From each macerated sample, 100 μl of sap was extracted with the Tri-Reagent (Molecular Research Center, Inc,...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... Real-time RT-PCR was performed using iScript™ OneStep RT-PCR Kit Probes in Chromo real time PCR machines (Bio-Rad, Hercules, CA, USA) The reaction was conducted in a total volume of 25 μl containing ... Research and Development, Ministry of Health, Jakarta, Indonesia for his support for this work, and Dr Maciej Boni from the Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 ... Thailand, 2004 Emerg Infect Dis 2005, 11(11):1664-1672 Boltz DA, Douangngeun B, Sinthasak S, Phommachanh P, Rolston S, Chen H, Guan Y, Peiris JS, Smith JG, Webster RG: H5N1 influenza viruses in...
Ngày tải lên: 12/08/2014, 04:21