... nuclear complexes These are the gateways to the nucleus A protein translated with a NLS will bind strongly to importin (aka karyopherin), and the complex will move through the nuclear pore (Fried ... The first is to make transgenic plants which overexpress a miRNA, typically under the control of strong 35S promoter (Jones-Rhoades et al., 2006) The second strategy is to make transgenic plants ... mechanical inoculation The youngest fully developed leaves from HCRSV-infected kenaf plants showing strong visual symptoms (0.1 g) was homogenized in 0.2 ml of 0.01 M phosphate buffer (pH 7.2) When...
Ngày tải lên: 10/09/2015, 09:03
... impurity due to tissue contamination, ACTIN7 was used as a negative control in RTPCR ACTIN7 is strongly expressed in vegetative tissues, but not expressed in pollen [21] No product was detected ... present (P)/absent (A) call after data normalization 4RT-PCR results were represented as follow: ++, strongly expressed; +, expressed; -, non-detectable 5Data for AGO8 was not reported in Pina et al...
Ngày tải lên: 12/08/2014, 03:20
Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
... by Herpes Simplex Virus (HSV) -2 [1] but also by HSV-1 [2] remains a worldwide problem [3] The strongest known risk factor for the heterosexual transmission of Human Immunodeficiency Virus (HIV)...
Ngày tải lên: 19/06/2014, 08:20
Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... at least 1,000-fold more sensitive than the traditional HA assay in detecting JCV There was a strong linear correlation between the HA assay and the DNA copy number of JCV(Mad1) either propagated...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
... by Herpes Simplex Virus (HSV) -2 [1] but also by HSV-1 [2] remains a worldwide problem [3] The strongest known risk factor for the heterosexual transmission of Human Immunodeficiency Virus (HIV)...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... at least 1,000-fold more sensitive than the traditional HA assay in detecting JCV There was a strong linear correlation between the HA assay and the DNA copy number of JCV(Mad1) either propagated...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
... retained in inflammatory breast cancer[31] Furthermore, derivative metastases frequently show strong E-cadherin expression[32] One emerging opinion is that dynamic, reversible modulation of E-cadherin...
Ngày tải lên: 09/08/2014, 07:21
Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc
Ngày tải lên: 12/08/2014, 02:20
báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
... interspecific Petunia hybrid [19]; it is characterized by vigorous growth, exceptional fertility, strong fragrance and white flowers V30 is an inbred line of modest growth habit and fertility featuring ... of 20 μL with oligo(dT)19 primer at 42°C for 50 V30 material Conclusions Altogether, there were strong similarities between the different programs but the coincidence in assigning best and worst...
Ngày tải lên: 12/08/2014, 03:21
Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot
Ngày tải lên: 12/08/2014, 04:20
Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps
... virus replication, assessed by viral infectivity and viral RNA production Together, these data strongly suggest that this current method can be a useful tool for rapid detection and quantification...
Ngày tải lên: 12/08/2014, 04:21
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