... reactions and the polyanionic nature of RNA, the catalytic chemistry and folding of group I ribozymes (and other large ribozymes) require divalent metals [11,12] Whereas only Mg2+ and Mn2+ are ... In (B), the kcat values are 1.1, 1.0 and 1.0 min)1, respectively, for the reactions at 0, 10 and 15 mM Mn2+, and the corresponding KG1/2 values are 22, 24 and 21 lM, respectively results also ... P7P3P8 domain, the 5Â strand of P7, which includes the G-binding site, is cleaved, whereas the 3Â strand is protected The 3Â strand of P3 is also protected, but the 5Â strand is neither protected...
Ngày tải lên: 19/02/2014, 07:20
... Casson and Gordon, and n = is the above corollary Indeed, if there exists a fibered ribbon knot whose classical Alexander module, first-order Alexander module and (n − 1)st -order Alexander module ... reviews intersection theory and defines Whitney towers and gropes Section defines (h)-solvability, and proves our theorems relating this filtration to gropes and Whitney towers In Section we prove ... Paper outline and acknowledgements Higher order Alexander modules and Blanchfield linking forms Higher order linking forms and solvable representations of the knot group Linking forms and Witt invariants...
Ngày tải lên: 14/03/2014, 22:20
gas - sensing properties of tin oxide doped with metal oxides and carbon
... temperature to LPG and ethanol gas are respectively shown in Fig 3a and b It seems that the optimized operating temperatures of the sensor to ethanol gas and LPG are around 350 and 250 ◦ C, respectively ... Environment and Chemistry, Hung-Yen University of Technology and Education His research activities are related with the design, fabrication and characterization of organic–inorganic hybrids and nanomaterials ... 320, 340, and 360 ◦ C) It should be noted that technological application requires the ethanol and LPG sensor to be able to detect at least 200 ppm alcohol (∼0.6 g/L in the human blood) and 0.24%...
Ngày tải lên: 19/03/2014, 16:48
hybrid metal oxide and multiwall carbon nanotube films for low temperature gas sensing
... in the presence of NO2 and CO both at ambient temperature and 150 ◦ C Three concentrations of each gas under study were measured: 100, 200 and 500 ppb for NO2 ; and 1, 10 and 50 ppm for CO The ... C1 and C2 correspond to 500 and 100 ppb (case of NO2 ) or 50 and ppm (case of CO) Table shows the sensitivities of the different sensors studied when operated at room temperature (25 ◦ C) and ... deposition (CVD), had purity higher than 95%, were up to 50 m in length and their outer and inner diameters ranged from 15 to nm and to nm, respectively A uniform functionalization with oxygen was...
Ngày tải lên: 19/03/2014, 16:48
Mechanical Estimating ManualSheet Metal, Piping and Plumbing pptx
... Cast Iron Hub and Spigot DWV 280 Section VI—CONTRACTING FOR PROFIT Chapter 20 Markups for Overhead and Profit 287 Understanding and Applying Correct Overhead and Profit Factors ... estimating, and has many sample estimates It shows you how to produce complete and accurate sheet metal, piping and plumbing estimates quickly and easily It contains complete man hours, material costs and ... competence and training, under average conditions, with typical equipment and xii tools, with normal motivation and supervision, should be able to and must in today’s market Where conditions and personnel...
Ngày tải lên: 22/03/2014, 13:20
Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx
... Chemicals and bacterial strains All chemicals were purchased from Sigma-Aldrich (St Louis, MO) Molecular biology reagents and enzyme were brought from Roche (Basel, Switerland), New England Biolabs ... 50 lgÆmL)1 kanamycin and increasing concentrations of TMP for the three rounds of evolution For the first round, lgÆmL)1 TMP was used, and in the second and third rounds, 10 and 20 lgÆmL)1 TMP were ... metalloenzymes, such as purple acid phosphatases and methionyl aminopeptidases [6–8] In vitro refolding Wild-type AMPP and AMPP#3-22 were overexpressed and purified Subsequently, the purified proteins...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
... site (His292, Tyr329 and Arg337; green), and substrate size-limiting residues (Pro306, Ser307 and Ile308; orange) and metal-chelating residues (Asp221, Asp232, His296, Glu325 and Glu339; cyan) are ... enzyme Enzyme activity was measured between pH and 10 (Fig 4) Activity was detected between pH 6.0 and 8.0 and reached a maximum at pH for both Leu-Pro and Arg-Pro The optimum pH was consistent with ... columns: Sephadex G-100 and G-150 (GE Healthcare) and Bio-Gel P-60 and P-200 (Bio-Rad Laboratories, Hercules, CA, USA) The size of the columns was 0.5 diameter · 10 cm, and 20 mm sodium citrate...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx
... 81, 177–183 Fernando LP, Wei D & Andrews GK (1989) Structure and expression of chicken metallothionein J Nutr 119, 309–318 Dalton T, Paria BC, Fernando LP, Huet-Hudson YM, Dey SK & Andrews GK (1997) ... (dashed line) and Zn3Cu7–MT1 (dotted line); (B) Cu–ackMT (black solid line), Cu–aMT4 (dashed line) and Cu–aMT1 (dotted line); and (C) Cu–bckMT (black solid line), Cu–bMT4 (dashed line) and Cu–bMT1 ... metallothionein L Villarreal et al Fig Structure and synteny of the chicken, mouse and human genomic regions containing the MT genes The chromosome number and bands enclosing the MT cluster are indicated...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH docx
... non-SDS/PAGE was strongly inhibited by Zn2+ and Cu2+, weakly inhibited by Ca2+ and Fe2+ and largely unaffected by Mg2+ Effect of pH and metal ions – CR binding and sedimentation analysis Because of ... 48 and 120 h, added to an equivalent volume of · tricine sample buffer and analysed by 15% Tris/tricine SDS- and native PAGE Proteins were electrophoretically transferred to nitrocellulose and ... Ab-immunoreactive bands with apparent molecular masses of 4-, 8-, 12- and 95-kDa were observed in samples incubated for 15 (Fig 2) After 24 h incubation, an increase in the 12- and 95-kDa species and the...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo hóa học: " Core shell hybrids based on noble metal nanoparticles and conjugated polymers: synthesis and characterization" ppt
... defined and stable polymeric shell Figure a: UV-Vis absorption spectra of samples Au@P (DMPAHCl) and Ag@P(DMPAHCl) and b: Ag, Au and Pt core shell in water suspensions image (yellow, pink and grey, ... contributions IV, IF and MVR carried out the synthesis and characterizations and drafted the manuscript, CC light scattering characterizations, CB and GP carried out XPS studies All authors read and approved ... Typical procedure to obtain hydrophobic and hydrophilic core shell hybrids The characteristic plasmon band for gold and silver has been observed at about 540 and 410 nm, respectively, with shoulders...
Ngày tải lên: 21/06/2014, 06:20
Mechanical Estimating Manual Sheet Metal, Piping and Plumbing doc
... Cast Iron Hub and Spigot DWV 280 Section VI—CONTRACTING FOR PROFIT Chapter 20 Markups for Overhead and Profit 287 Understanding and Applying Correct Overhead and Profit Factors ... estimating, and has many sample estimates It shows you how to produce complete and accurate sheet metal, piping and plumbing estimates quickly and easily It contains complete man hours, material costs and ... competence and training, under average conditions, with typical equipment and xii tools, with normal motivation and supervision, should be able to and must in today’s market Where conditions and personnel...
Ngày tải lên: 27/06/2014, 05:20
Mechanical Estimating ManualSheet Metal, Piping and Plumbing pot
... Cast Iron Hub and Spigot DWV 280 Section VI—CONTRACTING FOR PROFIT Chapter 20 Markups for Overhead and Profit 287 Understanding and Applying Correct Overhead and Profit Factors ... estimating, and has many sample estimates It shows you how to produce complete and accurate sheet metal, piping and plumbing estimates quickly and easily It contains complete man hours, material costs and ... competence and training, under average conditions, with typical equipment and xii tools, with normal motivation and supervision, should be able to and must in today’s market Where conditions and personnel...
Ngày tải lên: 27/06/2014, 18:20
Volume 07 - Powder Metal Technologies and Applications Part 1 pot
... materials and manufacturing methods The ASM Handbook Committee, the editors, the authors, and the reviewers have collaborated to produce a book that meets the high technical standards of the ASM Handbook ... compressibility and compaction, sintering practices, and atmosphere control An overview article, "Powder Shaping and Consolidation Technologies," also compares and summarizes the alternatives and factors ... Grace M Davidson, Manager of Handbook Production; Bonnie R Sanders, Copy Editing Manager; Alexandra B Hoskins, Copy Editor; Randall L Boring, Production Coordinator; and Kathleen S Dragolich, Production...
Ngày tải lên: 10/08/2014, 12:22
Volume 07 - Powder Metal Technologies and Applications Part 4 pot
... splintering (The heel of the hand is recommended in some standards and is an acceptable alternative if the hand can take it!) The rate of tapping should be about 150 taps per minute and the sieve should ... sampling methods using a 60:40 sand mixture Sampling technique Coning and quartering Scoop sampling Table sampling Chute slitting Rotary sample dividing Random variation Standard deviation, % 6.81 5.14 ... (homogeneous), and w = g For d95 = 20 m, = 1.5, P = 10-4 (100 ppm), = 0.05, l = 0.03 (random), and w = 8000 g References cited in this section P Gy, Sampling of Particulate Matter: Theory and Practice,...
Ngày tải lên: 10/08/2014, 12:22
Volume 07 - Powder Metal Technologies and Applications Part 5 ppsx
... penetrometer, and the sample References cited in this section S.J Gregg and K.S.W Sing, Adsorption, Surface Area and Porosity, Academic Press, 1982 35 Lowell and J.E Shields, Powder Surface Area and Porosity, ... understanding of the pore and network structure of the sample References cited in this section S.J Gregg and K.S.W Sing, Adsorption, Surface Area and Porosity, Academic Press, 1982 45 H.M Rootare and ... such as applied force and pressure, and properties of the resulting P/M product, such as strength and hardness, usually depend on the density of the materials being processed Standard industry practice...
Ngày tải lên: 10/08/2014, 12:22
Volume 07 - Powder Metal Technologies and Applications Part 6 pptx
... least complex, and class IV parts are the most complex To better understand the types of commercially available P/M compacting presses, and their advantages and limitations, an understanding of P/M ... electric hand tools and toothbrushes, cleats on sporting shoes, surgical tools such as scalpels, electrical connectors, handgun components, eyeglass and wristwatch components, golf clubs, and microwave ... Federation (MPIF) standard 47 details safety standards for P/M presses Mechanical presses are available in top-drive and bottom-drive arrangements In top-drive presses, the motor, flywheel, and gearing...
Ngày tải lên: 10/08/2014, 12:22
Volume 07 - Powder Metal Technologies and Applications Part 7 ppsx
... Cr2O3, and MgO; carbides such as Cr3C2, TiC, Mo2C and SiC (generally in a supporting metal matrix) and diamond; nitrides such as TiN and Si3N4, and spinels or perovskites such as mullite and superconducting ... attractive and repulsive forces surrounding the suspended particle A quantitative theory enabling determination of stability was developed independently by Deryagin and Landau and Verwey and Overbeek and ... bridges and other corrosion-prone in frastructure; petrochemical pumps and valves; and mining and agricultural equipment Except in the case of controlled-atmosphere spraying (VPS, inert chamber, and...
Ngày tải lên: 10/08/2014, 12:22
Volume 07 - Powder Metal Technologies and Applications Part 8 pdf
... temperature and sintering atmosphere (dissociated ammonia and nitrogen) and the strength increase due to nitrogen absorption Fig 21 (a) Effect of nitrogen content on ultimate tensile strength and elongation ... because corrosion resistance demands were modest and/ or corrosion resistance was not assessed Later, with increasing demands for improved corrosion resistance, and as more quantitative information ... and highly alloyed tool steel materials These advantages include uniform and finer microstructure, improved grindability, improved cutting performance, and capabilities of high-speed steels and...
Ngày tải lên: 10/08/2014, 12:22
Volume 07 - Powder Metal Technologies and Applications Part 9 ppsx
... wear resistance, burning and material transfer through arcing, and high-temperature strength, and (b) a matrix of an electrically and thermally conducting metal Tungsten and molybdenum are the ... produced: • • • • Ductile and tough roots and airfoil tips containing nearly 100% superalloy that withstood combined tensile and bend stresses at moderately high temperatures Ductile and tough superalloy-rich ... J.A Cornie, and K.C Russell, Infiltration and Wetting of Alumina Particulate by Aluminum and Aluminum-Magnesium Alloys, Metall Trans A, Vol 26, 1995, p 1491-1497 79 S Long, Z Zhang, and H.M Flower,...
Ngày tải lên: 10/08/2014, 12:22