... region Effect of mutations in the C-terminal regionofthe RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the functional features ofthe C-terminal regionof ... investigated the C-terminal regionofthe RmInt1 IEP up to the maturase domain andthe C-tail We found that the C-tail and upstream amino acid residues, located in a predicted a-helical region, form ... important regionofthe IEP maturase domain that is conserved throughout the evolution in the D lineage We show that C-tail ofthe RmInt1 IEP contributes to the maturase function of this RT protein and...
... part ofthe coenzyme B-binding site Its unmethylated nitrogen atom Ne2 donates the shortest ofthe three hydrogen bonds ofthe protein to the phosphate group of coenzyme B The distance of ˚ 2.65 ... because the genes required for the biosynthesis ofthe nickel cofactor F430 [16] and for the post-translational modifications [9] are not yet known [17,18] Also, the in vitro reconstitution of active ... or 2-methylglutamine The methylated histidine (H257) was found in the a-subunit of MCRs from all methanogenic archaea andofthe two MCRs from the methanotrophic archaea ofthe FEBS Journal 274...
... evolution ofthe primary structure ofthe N-terminal regions of TTR andthe biological function of TTR 4014 Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the ... T4) In the other, the N-terminal regionof crocTTR was deleted The affinity of T3 and T4 to both TTRs was studied andthe results are a powerful demonstration ofthe relationship between the evolution ... revealed that theregionofthe subunit which changed in a distinct and directed manner was the N-terminal regionThe ‘N-terminal region is defined as the amino acids from the N-terminus until the Cys...
... Asi iN -U 橋 d h ensa oH-O -AYU 篭車 一 F-u Uk ル KU ー K-s Utu b-U uS cuUk h a n E anD? k-S uTU B-u Us CUu H - ・ka N e And? 月 R 靴 ぶす rn a I cny ad big rde cosik hptcs dg o tI rn a g o mrig o d onn ... a δi , l s ocsoal,o h u o t i rvr w e h dos o of r o h c r w o ie y u hne f o l e h n e rp y u f o t t l k h gvs o cag.Iy u i e e v rt i ae i Koof g aot aig o o r rgt otd o Jpns c s s n yt ,o ... te ci n a s ses ts i Nvrhls,ery vr aaya ad fie ay n s k ad eetees nal eeyslrmn n ofc ld i O a a n truhu asihsland osek tnad aaee o ue n hogotKna a ere t pa sadr Jpns f s i r bsns,lhuh e r rnnito...
... several of these corepressors also interact with the C-terminal regions of CHD3 downstream of residue 1676 [46] or the conserved regions of CHD4 and dMi2 [50] Models proposed to explain the repression ... indicating the crucial importance of these two Ets motifs [15] In addition to controlling the level of gene expression, Ets factors may direct the choice ofthe promoter elements in play, and therefore, ... acids, the Ets domain, the CIDD andthe C-terminal domain The fragment of cDNA included in the bait used for two-hybrid screening ofthe brain cDNA library included amino acids 96–510 at the C-terminus...
... the current study, we sought to investigate the molecular basis ofthe differential expression of TPa and TPb isoforms Our initial aim was to map the patterns of exon usage in the 5¢ UTR ofthe ... number ofthe diverse superfamily of GPCRs, not least among the prostanoid receptor subfamily of GPCRs [53] The EP3 subtype ofthe PGE2 receptors is one ofthe most heavily spliced among all ofthe ... FEBS 2002 Analysis ofthe TPa and TPb mRNAs (Eur J Biochem 269) 4067 Fig Functional analysis ofthe 5¢ flanking regionofthe TP receptor gene (A) Schematic ofthe TP genomic region spanning nucleotides...
... as well as the deletions investigated, with the exception of deletion R30hE5, increased the number of colonies andthe colony size of keratinocytes growing in soft agar The effect ofthe empty ... The results shown in this communication demonstrate that the expression of theHPV16 E5 protein increases the number andthe size of HaCaT cell colonies growing in soft-agar The results were the ... but determines the number of cells and therefore the colony size (see Fig 6) The data here detailed represent the first quantitative description ofthe ability of HPV16 E5 to Page of 10 (page number...
... and will form the basis for evaluating the impact ofthe project over year period In May-June, Dr Mui andthe team from GRRC returned to the farms surveyed and initiated the introduction ofthe ... Bachthao bucks, to buy these bucks and circulate then around the project farms (and others) over the period ofthe project The issue here is determining the extent to which they are superior, it ... possible during the time ofthe project The project will depend on the continuous availability of a reliable vehicle and driver to gain access to the villages, and particularly for some ofthe remote...
... Bachthao bucks, to buy these bucks and circulate then around the project farms (and others) over the period ofthe project The issue here is determining the extent to which they are superior, it ... at the end ofthe project which will include the final outcome ofthe technological innovations on the productivity and well-being of Vietnamese goat farmers in these areas Objective Training and ... stage has been the training of DARD officers from Ninh Thuan, Binh Thuan and Lam Dong provinces in goat husbandry and management, in the conduct of surveys andthe acquisition of expertise to...
... diagnosis and treatment of goat diseases in Vietnam, training and information dissemination during the project andthe techniques developed and outcomes of pasture research over the years ofthe project ... activities, including analysis and interpretation of farming system surveys carried out in 2006 and 2008, andofthe biology and productivity of goats following the introduction of new technologies to ... Thuan and Lam Dong provinces of southeast Vietnam The title ofthe report is “New technologies for Improving Goat Production in Vietnam” and includes not only analysis and interpretation of the...
... stands were not included in the analyses because they not occur in theregionof Krušné hory Mts on the given sites In theregionof Bohemian-Moravian Upland the analyses included forest stands ... the same height from planting with severe and small root system deformations in theregionof Krušné hory Mts and in theregionof Bohemian-Moravian Upland (7) Mutual comparison of trees ofthe ... development and health condition were found either between the trees of identical height from self-seeding and from planting in theregionof Krušné hory Mts and in theregionof Bohemian-Moravian Upland...
... subcloned into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions ofthe 5 regionof GS1a to the GUS reporter gene The 981 bp sequence upstream ofthe translation ... [4,16,18], and none of them correspond to nitrogen metabolism We have studied the promoter activity ofthe 5’-untranslated region using fusions with the reporter gene uidA andthe presence of DNA-protein ... histochemical assays, the 5’upstream regionofthe pine gene was able to drive gene expression in pine cotyledons To further characterize the function ofthe 5’upstream regionofthe pine GS1a gene,...
... conducted the ELISA experiments and participated in the design ofthe study and analysis ofthe data FC participated in the design ofthe study and in the analysis of data and helped to draft the manuscript ... participated in the design and revision ofthe study AS participated in the analysis and interpretation of data and helped to draft the manuscript RR participated in the design ofthe study and in the revision ... the revision ofthe manuscript EP participated in analysis of data GV participated in the design ofthe study and in the revision ofthe manuscript MS conducted the experiments on endothelial cell...
... Acknowledgements The authors thank all the subjects and doctors for their cooperation in the study Authors’ contributions SA and MI conceived ofthe study participated in its design and coordination and gave ... (L) in variants of one ofthe breakthrough (BT1) sample (Table 1) and at position histidine (H) got replaced with Q in variants of one ofthe rapid responder (R1) (Tables 2) Other amino acid ... WJ, Changchien CS: Mutations in the NS5A and E2-PePHD regionof hepatitis C virus type 1b and correlation with the response to combination therapy with interferon and ribavirin J Viral Hepat 2003,...
... NH and OB conceived ofthe study, participated in its design, and in drafting the manuscript HT and JB participated in the study design and coordination and in discussing manuscript NH, NBF and ... described by Lindh et al in 1998 [26] The principal advantage of TSP-PCR is theregionofthe genome targeted by this method; in fact, TSP-PCR amplifies part ofthe S region which is known to be more ... erroneous results, the limit ofthe rapid methods using classic PCR is the low sensitivity of DNA detection (the limit of detection of our method being 10 copies/ml) This explains that the genotyping...
... evaluate the occurrence of calcified intervertebral discs in Finnish miniature dachshunds and to compare the relationship between the curvature oftheradiusand ulna with the degree of disc calcification ... that the curvature ofthe legs is proportional to the degree ofthe chondrodystrophy and by breeding straight-legged dachshunds the occurrence of IDD could be reduced The differences in the curvature ... Most ofthe sires had only one offspring included in this study (Fig 2) In both breeds there were 65% females and 35% males The ages and weights ofthe dogs are presented in Table According to the...
... population in the Archangel and Samara Oblasts and north-western regions of Russia and Estonia [8,27,29] It is therefore likely that the Beijing genotype is a major cause of tuberculosis and particularly ... typing ofthe isolates from the drug resistance survey was performed to determine the proportion of patients Figure DNA fingerprint and spoligotype patterns ofthe isolates obtained from five randomly ... from the characteristics ofthe complete study population ofthe drug resistance survey (data not shown) In brief, the final sample consisted of 239 male (60%) and 158 female (40%) patients The...
... results in the formation and/ or exposure of highly conserved regions previously folded into the core structure and/ or sheltered by the variable loops andthe glycans covering the outer domain of gp120 ... of infection by the SK3 anti-CD4 mAb (Figure and Tables and 3; only DS17 is shown but the other two clones had the same phenotype) In addition, the mutant Env generated in the background ofthe ... determinants of phenotypic changes Single mutants were constructed to test for the role of N283 and T283 on the phenotypes observed the identity ofthe gp120 and gp41 expressed from each construct Furthermore,...
... great impact on the health profile ofthe population [2,3] The ANC ofthe St Mary's Hospital Lacor is located in the Gulu district of North Uganda and has participated in the national HIV-1 sentinel ... (the National Institute of Health of Italy) and AVSI, in collaboration and with the approval ofthe STD/AIDS Control Programme ofThe statistical analyses were conducted excluding data for the ... in the North-Central regionof Uganda, which also includes the districts of Lira and Apac [4] About 10% of people living in the Acholi region are resident in urban areas and approximately 90%...
... minorities, andthe organisations that work with them, have not received the attention and support of their counterparts in southern parts ofthe country This has the potential to further limit the ability ... understand the climate vulnerability and capacity of ethnic minorities in the northern mountains of Vietnam it is vital to understand these causes The analysis illustrates the complexity of vulnerability ... populated provinces in the northern midlands and mountain areas of Vietnam Bac Kan province is one ofthe poorest provinces the northern mountainous Regionand in Vietnam overall The poverty rate in...