regulation of nf e2 related factor 2 activity

... Immunoblotting 20 2. 6 Luciferase reporter assay 20 2. 7 Immunoprecipitation 21 2. 8 Immunoflourescence 21 2. 9 Collection and processing of plants 22 2. 10 Growth and isolation of endophytes 22 2. 11 Molecular ... al., 20 06   12     Ub Ub Ub Nrf2 ET GE KEL CH DLG Ub KELCH C288 IVR C288 C273 IVR C273 BTB C151 BTB C151 E2 E2 E2 Keap1 Ub Rbx1 Cullin3 Figure 1 .2 Schematic representation of the binding of Keap1 ... protein MAPK Mitogen-activated protein kinases NF- E2 Nuclear factor erythroid -2 NF- κB Nuclear Factor- kappaB Nrf2 Nuclear factor erythroid -2 (NF- E2) -related factor Nqo1 NAD(P)H:quinine oxidoreductase...

Ngày tải lên: 09/09/2015, 08:16

... generation JAMA 29 6, 397–4 02 22 Stegenga ME, van der Crabben SN, Levi M, de Vos AF, Tanck MW, Sauerwein HP & van der Poll T 328 8 23 24 25 26 27 28 29 30 31 32 33 34 35 36 (20 06) Hyperglycemia stimulates ... Journal 27 6 (20 09) 327 7– 328 9 ª 20 09 The Authors Journal compilation ª 20 09 FEBS P Totta et al FGF -2 is a thrombin substrate A p-ERK1 /2 Total ERK1 /2 p-ERK2 / total ERK2 B 1.5 1.0 0.5 FGF -2 Thrombin ... 374, 123 – 129 FEBS Journal 27 6 (20 09) 327 7– 328 9 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 328 7 FGF -2 is a thrombin substrate P Totta et al Abuharbeid S, Czubayko F & Aigner A (20 06) The...

Ngày tải lên: 16/03/2014, 01:20

... (IFITM1) Hs016 525 22_ g1 Interferon-stimulated gene, 15 kDa (ISG15) Hs001 927 13_m1 Interferon regulatory factor (IRF7) Hs0 024 2190_g1 2' ,5'-oligoadenylate synthetase (OAS1) Hs0 024 2943_m1 2' ,5'-oligoadenylate ... cytokines and E2 This encouraged us to further examine the effects of interactions between IFN and one of TNF, IFN, or E2 on IFI gene expression Gathering the above results, TNF, IFN, and E2 were ... arthritis identified by DNA maicroarray analysis Ann Rheum Dis 20 09, 68 :26 4 -27 2 19 20 21 22 23 24 25 26 27 28 29 Baechler EC, Batliwalla FM, Karypis G, Gaffney PM, Ortmann WA, Espe KJ, Shark KB,...

Ngày tải lên: 09/08/2014, 01:22

... (IFITM1) Hs016 525 22_ g1 Interferon-stimulated gene, 15 kDa (ISG15) Hs001 927 13_m1 Interferon regulatory factor (IRF7) Hs0 024 2190_g1 2' ,5'-oligoadenylate synthetase (OAS1) Hs0 024 2943_m1 2' ,5'-oligoadenylate ... cytokines and E2 This encouraged us to further examine the effects of interactions between IFN and one of TNF, IFN, or E2 on IFI gene expression Gathering the above results, TNF, IFN, and E2 were ... arthritis identified by DNA maicroarray analysis Ann Rheum Dis 20 09, 68 :26 4 -27 2 19 20 21 22 23 24 25 26 27 28 29 Baechler EC, Batliwalla FM, Karypis G, Gaffney PM, Ortmann WA, Espe KJ, Shark KB,...

Ngày tải lên: 09/08/2014, 13:22

... experiments Control +S1P +Anisomycin Y -27 6 32 ) ) + – + – + G-Actin/F-Actin 0 .26 0 ± 0. 023 0.181 ± 0. 023 * 0 .22 ± 0. 021 0 .23 9 ± 0.018 0 .22 3 ± 0.013 0.110 ± 0.034** 0 .22 7 ± 0.019  *P < 0.05, **P < 0.01 ... Allen, T.J (20 02) Prevention of accelerated atherosclerosis by 18 19 20 21 22 23 24 angiotensin-converting enzyme inhibition in diabetic apolipoprotein E-deficient mice Circulation 106, 24 6 25 3 Chaqour, ... Control SIP +SIP + Y276 32 +SIP + SB -20 3580 Incubation Time (hrs) 1.64 - 80 1.40 4.6 - 1 .23 3 .29 +Serum 100 CTGF GAPDH 2. 5 +Anisomycin 120 CTGF GAPDH 1.35 +S1P B CT Control +Y -27 6 32 +SB -20 3580 GF CT...

Ngày tải lên: 19/02/2014, 16:20

... phosphorylation of ErbB2 [100] The Ca2+ ⁄ CaM complex also negatively regulates the tyrosine kinase activity of ErbB2 by an FEBS Journal 27 7 (20 10) 327 –3 42 ª 20 09 The Authors Journal compilation ª 20 09 ... AC & Davis RJ (19 92) Mechanism of desensitization of the epidermal growth factor receptor protein-tyrosine kinase J Biol Chem 26 7, 1 129 –1140 FEBS Journal 27 7 (20 10) 327 –3 42 ª 20 09 The Authors ... multiple levels [21 ,22 ] This Ca2+ signal occurs in multiple cell types [23 28 ] The cytosolic Ca2+ rise is followed by an increase in the concentration of free Ca2+ in the nucleus [28 –30], and this...

Ngày tải lên: 06/03/2014, 09:22

... 115, 26 25– 26 32 31 Kucharczak J, Simmons MJ, Fan Y & Gelinas C (20 03) To be, or not to be: NF- kappaB is the answer – role of Rel ⁄ NF- kappaB in the regulation of apoptosis Oncogene 22 , 8961–89 82 ... inhibi55 42 Fig Knockdown of NF- jB1 inhibits growth of ES -2 cells in vitro (A) The validity of pSilencer ⁄ si -NF- jB1 (si -NF- jB1) was examined in ES -2 cells by western blotting with anti- (NF- jB1) ... microRNA targets Genes Dev 18, 1165– 1178 29 Hayden MS & Ghosh S (20 04) Signaling to NF- kappaB Genes Dev 18, 21 95 22 24 30 Luo JL, Kamata H & Karin M (20 05) IKK ⁄ NFkappaB signaling: balancing life and...

Ngày tải lên: 07/03/2014, 00:20

... hoPL (Fig 2A, lane 3), hoPLRP2 ran as two fragments of 27 .5 and 22 .5 kDa (Fig 2A, lane 4), in agreement with previous results demonstrating the high sensitivity of the hoPLRP2 Ser244–Thr245 bond ... case of NcC2, no inhibition of the lipase activity was observed, as 100% of the lipase activity still remained even at a molar excess of 45 The effect of NcC2 was similar to that of E600-hoPLRP2 ... hoPLRP2 and N2C2, and conversely, hoPLRP2 antibodies reacted better with hoPLRP2 and N2C2 than they did with hoPL and Fig Functional maps of the plasmids expressing natural and chimeric isoforms of...

Ngày tải lên: 16/03/2014, 06:20

... Rifkin DB & FEBS Journal 27 5 (20 08) 1579–15 92 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 1591 Intracellular trafficking of FGF -2 19 20 21 22 23 24 25 26 27 28 29 30 31 S Taverna et al ... Journal 27 5 (20 08) 1579–15 92 ª 20 08 The Authors Journal compilation ª 20 08 FEBS c B 120 100 C Control Ouabain 80 60 40 20 120 Number of FGF -2 granules b 100 D Control Ouabain 80 60 ** 40 20 proteins ... Journal 27 5 (20 08) 1579–15 92 ª 20 08 The Authors Journal compilation ª 20 08 FEBS S Taverna et al Analysis of the intracellular localization of FGF -2 and of lipidic structures To verify whether FGF-2...

Ngày tải lên: 16/03/2014, 06:20

... http://arthritis-research.com/content/11/1 /21 5 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 HIF prolyl-hydroxylase is the key oxygen sensor setting low α steady-state levels of HIF-1α in normoxia EMBO J 20 03, 22 :40 82- 4090 Ivan ... Upregulation of hypoxiainducible factors in normal and psoriatic skin J Invest Dermatol 20 07, 127 :24 45 -24 52 62 Chen LW, Egan L, Li ZW, Greten FR, Kagnoff MF, Karin M: The κ two faces of IKK and NF- κB ... hypoxia in a number of studies [ 12, 18,19] Cyclooxygenase [20 ], TNFα [21 ], IL-6 [22 ] and macrophage inflammatory protein [23 ] are among the target genes identified for hypoxia-induced NF B, and these...

Ngày tải lên: 09/08/2014, 01:22

... of nuclear factor kappa B (NFB): an emerging theme in anti-inflammatory therapies Mol Interv 20 02, 2: 22- 35 Tak PP, Firestein GS: NF- kappaB: a key role in inflammatory diseases J Clin Invest 20 01, ... or professional service and there are no personal financial holdings that could be perceived as constituting a potential conflict of interest 23 24 25 Received: 31 October 20 09 Accepted: 20 January ... RT: Thioredoxin regulates the DNA binding activity of NF- kappa B by reduction of a disulphide bond involving cysteine 62 Nucleic Acids Res 19 92, 20 :3 821 -30 Mitomo K, Nakayama K, Fujimoto K, Sun...

Ngày tải lên: 11/08/2014, 08:22

... Interference of hSKP1 and hSRB7/MED21 63 2. 2.1 Design of siRNA pSuper Constructs 63 2. 2 .2 Annealing of Constructs 63 2. 3 Transfection of High Quality Plasmids into HeLa Cells 64 iv 2. 3.1 Retransformation ... Immunoprecipitation of hCul1 122 Figure 20 : Immunoprecipitation of hMed6 123 Figure 21 : Co-immunoprecipitation of Cul1 by Med6 with Protein A and Protein G 124 Figure 22 : Co-immunoprecipitation of Med6 by ... Vectors 60 2. 1.5 Transformation and Inoculation 60 2. 1.6 Low-yield Purification of Plasmid DNA (Miniprep) 61 2. 1.7 Qualification of Miniprep product 62 2.1.8 DNA Sequencing 62 2 .2 Synthesis of Constructs...

Ngày tải lên: 10/09/2015, 08:27

... Conversely, treatment of pVvMYB30:GUS plants with ABA did not Galbiati et al BMC Plant Biology 20 11, 11:1 42 http://www.biomedcentral.com/1471 -22 29/11/1 42 Page of 14 Figure Activity of the grape VvMYB60 ... Sequence of the Genome of a Heterozygous Grapevine Variety PloS ONE 20 07, 2( 12) :e1 326 12 Matus JT, Aquea F, Arce-Johnson P: Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality -related ... Biol 20 08, 8:(83) 13 Kranz HD, Denekamp M, Greco R, Jin H, Leyva A, Meissner RC, Petroni K, Urzainqui A, Bevan M, Martin C, et al: Towards functional characterisation 22 23 24 25 26 27 28 29 30...

Ngày tải lên: 11/08/2014, 11:21

... Chem 20 03, 27 8 :27 224 -27 229 26 Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T: CREB-binding protein/p300 are transcriptional coactivators of p65 Proc Natl Acad Sci USA 1997, 94 :29 27 -29 32 ... decreases TNF-α-induced binding of NF- κB to DNA [22 ] Consistent with these results, administration of atRA reduces joint destruction in collagen-induced inflammatory arthritis [23 ] Inhibitor of NF- κB ... basal NF- κB activity However, atRA significantly inhibited TNF-α-activated NF- κB activity, suggesting that active RARs suppress NF- κB activity We next evaluated whether activation of NF- κB influences...

Ngày tải lên: 09/08/2014, 10:22

... the nature of the ERP, the search for clinical integration will assuredly intensify Conflict of Interests The authors have declared that no conflict of interest exists 19 20 21 22 23 24 References ... Research 20 05; 22 (2) :22 1 -23 1 80 Barabasz A, Barabasz M, Jensen S, et al Cortical event -related potentials show the structure of hypnotic suggestions is crucial The International Journal of Clinical ... Picton TW N2 and automatic versus controlled processes Electroencephalography and Clinical Neurophysiology 1986; 38:169-186 25 26 27 28 29 Näätänen R The role of attention in auditory information...

Ngày tải lên: 02/11/2012, 11:08

... characterization of a second AP -2 transcription factor: AP -2 beta Development 121 , 27 79– 27 88 Chazaud C, Oulad-Abdelghani M, Bouillet P, Decimo D, Chambon P & Dolle P (1996) AP -2. 2, a novel gene related ... domains of AP -2 family transcription factors J Biol Chem 27 5, 29 701 29 708 10 Williams T & Tjian R (1991) Analysis of the DNAbinding and activation properties of the human transcription factor AP -2 ... Dev 5, 670–6 82 11 Eckert D, Buhl S, Weber S, Jager R & Schorle H (20 05) The AP -2 family of transcription factors Genome Biol 6, 24 6, doi:10.1186/gb -20 05-6-13 -24 6 12 Moser M, Ruschoff J & Buettner...

Ngày tải lên: 16/02/2014, 09:20

... (20 07) Heat shock factor (HSF2) contrib- Regulation of HSFs 17 18 19 20 21 22 23 24 25 26 27 28 29 utes to inducible expression of hsp genes through interplay with HSF1 J Biol Chem 28 2, 7077–7086 ... localization of the heat shock factor isoforms in the rat seminiferous epithelium Exp Cell Res 24 0, 16 27 121 Schwob JE (20 02) Neural regeneration and the peripheral olfactory system Anat Rec 26 9, 33–49 ... C (1993) Regulation of heat shock factor trimer formation: role of a conserved leucine zipper Science 25 9, 23 0 23 4 Sakurai H & Enoki Y (20 10) Novel aspects of heat shock factors: DNA recognition,...

Ngày tải lên: 18/02/2014, 04:20

... the presence of various concentrations of 2A2 mAb The binding of 2A2 mAb was followed by native gel electrophoresis Increasing the concentration of 2A2 mAb at a fixed molar ratio of cathepsin ... for 2A2 mAb, and the Kd for the interaction between 2A2 FEBS Journal 27 6 (20 09) 4739–4751 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 4741 ´ B Mirkovic et al Regulation of cathepsin B activity ... binding 2A2 mAb The lack of dissociation of cathepsin FEBS Journal 27 6 (20 09) 4739–4751 ª 20 09 The Authors Journal compilation ª 20 09 FEBS 4745 ´ B Mirkovic et al Regulation of cathepsin B activity...

Ngày tải lên: 18/02/2014, 11:20

... Journal 27 3 (20 06) 5333–5346 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y Ding et al 24 25 26 27 28 29 30 31 32 33 34 35 36 Yoshida T (1995) Heme oxygenase -2 Properties of the heme complex of ... Chem 27 4, 26 071 26 078 Sun J, Brand M, Zenke Y, Tashiro S, Groudine M & Igarashi K (20 04) Heme regulates the dynamic exchange of Bach1 and NF- E2- related factors in the Maf transcription factor ... [22 ] HO -2 contains the cysteine and proline (CP) motifs [23 ], whereas HO-1 lacks a cysteine residue [24 ,25 ] Each CP motif of HO -2 may function as a heme-binding site [26 ], suggesting that HO-2...

Ngày tải lên: 19/02/2014, 05:20

... tight binding FEBS Journal 27 3 (20 06) 615– 627 ª 20 06 The Authors Journal compilation ª 20 06 FEBS S Ahmed et al 21 22 23 24 25 26 27 28 29 30 31 32 to soluble form of Alzheimer amyloid precursor ... essential for its gelatinase A-inhibitory activity J Biol Chem 27 8, 14 020 –14 028 FEBS Journal 27 3 (20 06) 615– 627 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 627 ... the presence of 10 mm CaCl2, as described in Experimental procedures Of the nine cell lines tested, OVISE, FEBS Journal 27 3 (20 06) 615– 627 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 617...

Ngày tải lên: 19/02/2014, 07:20