Plant Breeding 132, 237–245 (2013) © 2013 Blackwell Verlag GmbH doi:10.1111/pbr.12053 Review Molecular markers for late blight resistance breeding of potato: an update J A G E S H K T I W A R I 1,4, S U N D A R E S H A S I D D A P P A 1, B I R P A L S I N G H 1, S U R I N D E R K K A U S H I K 2, S W A R U P K C H A K R A B A R T I 1,3, V I N A Y B H A R D W A J and P O O N A M C H A N D E L 1 Central Potato Research Institute, Shimla, 171 001, Himachal Pradesh India; 2Central Potato Research Institute Campus, Modipuram, Meerut, 250 110, Uttar Pradesh India; 3Present address: Director, Central Tuber Crop Research Institute, Thiruvananthapuram - 695 017, Kerala, India; 4Corresponding author, E-mail: jageshtiwari@gmail.com With tables Received October 29, 2011/Accepted January 19, 2013 Communicated by P Wehling Abstract Late blight is the most devastating disease of the potato crop that can be effectively managed by growing resistant cultivars Introgression of resistance (R) genes/quantitative trait loci (QTLs) from the Solanum germplasm into common potato is one of the plausible approaches to breed resistant cultivars Although the conventional method of breeding will continue to play a primary role in potato improvement, molecular marker technology is becoming one of its integral components To achieve rapid success, from the past to recent years, several R genes/QTLs that originated from wild/cultivated Solanum species were mapped on the potato genome and a few genes were cloned using molecular approaches As a result, molecular markers closely linked to resistance genes or QTLs offer a quicker potato breeding option through marker-assisted selection (MAS) However, limited progress has been achieved so far through MAS in potato breeding In near future, new resistance genes/QTLs are expected to be discovered from wild Solanum gene pools and linked molecular markers would be available for MAS This article presents an update on the development of molecular markers linked to late blight resistance genes or QTLs by utilization of Solanum species for MAS in potato Key words: late blight — molecular marker — MAS — potato — resistance gene — Solanum species Late blight caused by the oomycete (Phytophthora infestans (Mont.) de Bary) is the most important disease of potato production worldwide This disease caused devastating impact on humanity in the mid-1840s when severe epidemics swept through Europe and resulted in the Irish potato famine (Fry 2008) Consequently, given its significant importance, there have been concerted global efforts for more than 100 years to develop durable resistant potato cultivars against P infestans However, evolution of new races of P infestans was able to conquer the past resistance genes and resulted in susceptible cultivars worldwide Durably resistant cultivars against a range of P infestans isolates possessing multiple resistance genes are needed today, which can be developed in less time by conventional and molecular approaches Regardless of the fact that common potato lacks significant sources of resistance, many wild Solanum species are rich sources of resistance genes Globally breeders exploited only a very limited scale of Solanum biodiversity in potato breeding For example, the genetic base of modern Indian potato cultivars is limited to 49 ancestors only involving the wild species S rybinii and S demissum Late blight resistance genes were introgressed from the wild species S demissum, S stoloniferum and the cultivated S tuberosum subsp andigena and S phureja into common potato in different parts of the world (Bradshaw et al 2006c) Thus, it necessitates potato breeders to search for new sources of resistance in wild gene pools and their faster deployment into cultivars through markerassisted selection (MAS) Conventional breeding methods are of primary importance but are too slow (10–15 years) because they are essentially based on several generations of back crossing, field evaluation 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Domkarova, and J Zoufala, 2005: Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers Plant Soil Environ 51, 82—86 Sliwka,... Inheritance of resistance to late blight In: J E Bradshw, and G R Mackay (eds), Potato Genetics, 365—401 CAB International Wallinford, UK Verzaux, E., 2010: Resistance and Susceptibility to Late Blight. .. wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato Plant J 36, 867—882 Molecular markers for late blight resistance