Triển vọng ứng dụng của sợi nấm sò vàng pleurotus citrinopileatus được nuôi cấy dịch thể trong nuôi trồng nấm và sản xuất các sản phẩm dược liệu

ĈҤI HӐ& Ĉ¬ ҸNG1 75Ѭ Ӡ1*ҤĈ I HӐ& 6Ѭ3+ ҤM KHOA SINH ±0Ð, 75Ѭ ӠNG 1*Ð7+ ӎHӖ1*9Ỉ1 ³TRIӆN VӐNG ӬNG DӨNG CӪA SӦI NҨ06Đ9¬1* (Pleurotus citrinopileatus) ĈѬӦ&18Ð, ҨY Dӎ & CH THӆ 7521* 18Ð, ӖNG NҨ 75 0 9¬6 ҦN XUҨ7 &È&6 ҦN PHҬ0'Ѭ ӦC LIӊU´ Ĉj ҹ ng 1- NăP  ĈҤI HӐ& Ĉ¬ ҸNG1 75Ѭ Ӡ1*ҤĈ I HӐ& 6Ѭ3+ ҤM KHOA SINH ±0Ð, 75Ѭ ӠNG 1*Ð7+ ӎHӖ1*9Ỉ1 ³TRIӆN VӐNG ӬNG DӨNG CӪA SӦI NҨ06Đ9¬1* (Pleurotus citrinopileatus) ĈѬӦ&18Ð, ҨY Dӎ & CH THӆ 7521* 18Ð, ӖNG NҨ 75 0 9¬6 ҦN XUҨ7 &È&6 ҦN PHҬ0'Ѭ ӦC LIӊU´ 1JjQK&{QJQJK Ӌsinh hӑc *LiRYLrQKѭ ӟng dүn: ThS NguyӉ n Thӏ%tFK+ ҵng Ĉj ҹ ng 1- NăP  LӠ,&$0 Ĉ2$1 7{LFDP ÿRDQÿk\OjF{QJWUuQKQJKLrQ ӭu cӫ a ULrQJW{L &iFV ӕliӋ u, kӃ t quҧQrXWURQJ ұ Q OjWUXQJ NKyDOX ӵFYj ӯQJ FKѭDW WKӧFDL ÿѭ F{QJ bӕtrong bҩ t kǤF{QJWUuQKQjRNKiF Ĉj ҹ QJ 03 QJj\ WKiQJ 05 QăP  1J{7K ͓H͛QJ9kQ LӠI CҦ0 Ѫ1 ĈӇKRjQ WKjQK ұ n tӕt nghiӋ NKyD p cӫ DOX PuQK W{L ӱi[LQ lӡi cҧ P JѫQ FKkQ W nhҩ Wÿ Ӄ n tұ p thӇJLiR YLrQWURQJ ±0{L ӡQJĈ WUѭ NKRD6LQK ҥ i hӑF6ѭ ҥ PĈj SKҹ ng ÿmK Ӄ WOzQJW ұ QWuQKFK Ӎbҧ o, truyӅ Qÿ ҥ t kiӃ n thӭFFKRW{LWURQJVX ӕWTXiWUuQK ӑc tұ p tҥ LWUѭ ӡng thӡi gian qua Ĉһ c biӋ W W{L ӓOz [LQ ng biӃ W Ej\ ѫQ ҳ F W VkX Ӄ nÿThS V NguyӉ n Thӏ%tFK ҵ ng, + QJѭ ӡL ÿm ӵc tiӃ S WUӟ Kѭ ng dү n, tұ Q WuQK ӥYj JL~S ӝQJ ÿ YLrQ ÿ Fi QKkQ ӕt W TXi WUuQKWK ӵc hiӋ Q NKyD ұ n tӕt nghiӋ OX S Qj\ Thұ W OzQJҧ P Y{ ѫQ ӵ FQJ hӛtrӧ V F JL~S ӥYj ӝQJ ÿ YLrQ ӫ D JLD F ÿuQK ҥ n W EqWURQJVX ӕWTXiWUuQKKRjQWKjQKNKyDOX ұ n tӕt nghiӋ SFNJQJQKѭWURQJ ӕWTXiWUu hӑc tұ p vӯa qua Mһ FGÿmF ӕgҳ ng hӃ t sӭFÿ ӇKRjQ WKjQKNKyDOX ұ n song sӁNK{QJWK ӇWUiQK ӓi NK nhӳng thiӃ X VyW tQK ҫ \ PRQJ F{ JLiR TXê Ӈbҥ WK Q FQJ Eqÿ WRjQ JyS ӇÿӅWjL êWK ÿѭ ӧFKRjQ Ӌ QKѫQ WKL ;LQNtQKFK~FTXêWK ҫ \F{ ӭc khӓ V HYjWKjQKF{QJ ӵnghiӋ SÿjRW ҥ oWURQJV nhӳng thӃhӋtri thӭc tiӃ SWKHRWURQJWѭѫQJ ODL Mӝt lҫ n nӳD [LQFKkQ ҧ P ѫQ WKjQK F Ĉj ҹ QJ 03 QJj\ WKiQJ 05 QăP  6LQKYLrQWK ͹c hi͏ n 1J{7K ͓H͛QJ9kQ MӨC LӨC DANH MӨC CHӲ VIӂT TҲT DANH MӨC BҦNG BIӆU DANH MӨ& +Ỵ1+ Ҧ1+ Ӗ THӎ Ĉ MӢĈҪU Ĉ һ t vҩ Qÿ Ӆ Mө FÿtFKQJKL rQF ӭu éQJKƭDNKRDK ӑFYjWK ӵc tiӉ n cӫDÿ ӅWjL éQJKƭDNKRDK ӑc éQJKƭDWK ӵc tiӉ n &+ѬѪ1*7 Ә1*48$17¬,/, ӊU 1.1 TӘ1*48$17¬,/, ӊU 1.1.1 Nҩ PVz .4 1.1.2 Nҩ PVz YjQJ 7ẻ1++ẻ1+1*+,ầ1& 87521*9ơ1*2ơ,1 C 11 7uQKKuQKQJKLrQF ӭXWURQJQѭ ӟc 11 7uQKKuQKQJKLrQF ӭXQJRjLQѭ ӟc 11 &+ѬѪ1*Ĉ Ӕ,7Ѭ NG, N,'81*9ơ3+1*3+ẩ31*+ U14  ,7 1*1*+,ầ1& U 14 2.2 NӜ,'81*1*+,Ç1& ӬU 14 3+ѬѪ1*3+È31*+,Ç1& ӬU 14 2.3.1 Khҧ R ViWVLQKWUѭ ӣng cӫ a hӋsӧi nҩ P VzYjQJ ӡng WUrQP{LW dӏ ch thӇ QX{LF ҩ \WƭQKYjO ҳ c 14 3KѭѫQJSKiS[iFÿ ӏ nh sinh khӕLNK{ 15 3KѭѫQJ ҧ PTXDQ SKiS ÿiQK Yj TXDQ JLi Ӌsӧi nҩ ViWKuQK F P Vz YjQJ 16 ;iFÿ ӏ QKWKjQKSK ҫ QGLQKGѭ ӥng quҧthӇYjWѫQ ҩ PVzYjQJ 16 ;iF ӏ QKGѭ ӧc chҩ WFyWURQJK ÿ Ӌsӧi nҩ m, quҧthӇVzYjQJ ӏ ch thӇYj G VDXQX{LF ҩ y 17 ;iFÿ ӏ nh chӍWLrXYLVLQKY ұ t hӋsӧi nҩ PVzYjQJ 20 3KѭѫQJSKiSQX{LWU ӗng thӱnghiӋ m 21 3KѭѫQJSKiSSKkQWtFKV ӕliӋ u 22 &+ѬѪ1* ӂT QUҦ9¬%¬1/8 ҰN 23 3.1 KHҦ2 6È7 6,1+ NG C 75 A HSI N0 6ẹ9ơ1* 75ầ1 0é 75Ѭ ӠNG Dӎ CH THӆ 23 3.1.1 Khҧ R ViW ҧ QK ӣng Kѭ cӫ D P{LWUѭ ӡQJ GLQK ӥQJ Ӄ QVLQKWUѭ ÿ Gѭ ӣng hӋsӧi nҩ PEjRQJѭYjQJWURQJP{LWUѭ ӡng dӏ ch thӇQX{LF ҩ \WƭQKYjO ҳ c 23 3.1.3 Khҧ R ViWV ӵҧ QK ӣng Kѭ cӫ DS+ Ӄ QVLQKWUѭ ÿ ӣng hӋsӧLVzYjQJ WU P{LWUѭ ӡng dӏ ch thӇQX{LF ҩ \WƭQK 25 3.2 KӂT QUҦ ĈÈ1+ *,È Ҧ0 48$1 & 9ơ 48$1 6ẩ7 S +ẻ1+ I N06ẹ9ơ1* 28 ĈiQK ҧ m quan bҵ QJ JLi SKѭѫQJ F SKiS ҳ c, trҥ TXDQ QJ WKiL ViWP Y ÿӝGj\F ӫa hӋsӧi 28 4XDQViWKuQKWKiLK Ӌsӧi nҩ PVzYjQJ 29 3.3 KӂT QUҦ 18Ð, ӖNG 75THӰ NGHIӊM GIӔNG DҤNG LӒNG SO VӞI DҤNG HҤT TRUYӄN THӔNG 29 ;È& ӎ NH 7+¬1+ Ĉ Ҫ1 3+',1+ ӤNG'Ѭ TRONG QUҦ THӆ9¬ 7Ѫ NҨ06Đ9¬1* 31 ғ ғ Ѵ    ;$ ғ & Ĉ, ҕ + 'ѬѪ& ҕ &+ặ 7&2  7521* +ầ 6,  1ặ 0  48$  7+ầ  %$ 2 Ѵ ғ 1*Ѭ 9$ Ғ 1* 9$ Ғ  ', & + 7+ầ  6$8 18é,  &ặ < 33     r ғ W  TXD  ÿL Q ҕ K  O ѭѫQ ҕ J  polysaccharide 33 Ѵ     r ғ W  T X D  [ D F  ÿL Q ҕ K  K RD W ҕ  W Õ Q K  N K D Q J  N K X k Ѵ Q  F X D  K r  ҕ V ѫL ҕ  Q k ғ P Y D  T X D  W K r Ѵ  E D R Ѵ ғ ғ Ѵ Ғ Ѵ Ғ ғ Q Jѭ Y D QJ 36 Ғ     r ғ W  T X D  [ D F  ÿL Q ҕ K  K RD W ҕ  W Õ Q K  N K D Q J  R[ L  K yD  F X D  K r  ҕ V ѫL ҕ  Q k ғ P Y D  T X D  W K r Ѵ  Ѵ ғ ғ Ѵ Ғ Ѵ ғ E D R QJ ѭ Y D QJ 37 Ғ Ғ 3.5.4 KӃ t quҧÿӏ QKWtQK ӝt sӕhӧp chҩ P W FyKR ҥ W WtQKVLQKK ӑc quҧthӇ YjWѫQ ҩ PVzYjQJ 39 ғ ;$ғ & ӌ  7, Ç Ĉ, 8 9, ҕ  1+ , 1+ &&+ ғ  7521* +ầ 6,  1ặ 0 1* 9$ 1* 41 KӂT LUҰ19¬., ӂN NGHӎ 43 7¬,/, ӊU THAM KHҦO 45 PHӨLӨC 50 DANH MӨC CHӲ VIӂT TҲT PD+ Potato Dextro cҧ i tiӃ n PDR+ Potato Dextro cҧ i tiӃ QUѫP PDC+ Potato Dextro cҧ i tiӃ QFiP BE Biology effective PE Polietilen WSPS Water Solution Polysaccharide PSK Polysaccharide Krestin DANH MӨC BҦNG BIӆU 6{ғ KLr ҕX ED QJ Ѵ 7r Q ED QJ Ѵ Trang HiӋ u quҧNKiQJ XQJ WKѭ ӣ chuӝ 6DUFRPD t cӫ a  1.1 SRO\VDFFKDULGH ӟF WDQ WiFK ӯ nҩ mWURQJ P W Qѭ citrinopileatus HiӋ u quҧNKiQJ XQJ WKѭ ӣ chuӝ 6DUFRPD t cӫ a  1.2 SRO\VDFFKDULGH ӟF NK{QJ WUtFK ӯnҩ WDQ m O\ 10 WURQJ W Qѭ P citrinopileatus ҦQKKѭ ӣng cӫ DP{LWUѭ ӡQJGLQKGѭ ӥQJÿ Ӄ QVLQKWUѭ ӣng 3.1 hӋsӧi nҩ m VzYjQJ WUrQ P{L ӡngWUѭ dӏ ch thӇQX{L ҩ y F 23 WƭQK Yj ҳ c O 3.2 ҦQKKѭ ӣng cӫ a pH ÿӃ QVLQKWUѭ ӣng hӋsӧi nҩ m VzYjQJ 26 WURQJP{LWUѭ ӡng dӏ ch thӇQX{LF ҩ \WƭQKYj ҳ c O 3.3 ҦQK ӣng Kѭ cӫ a loҥ i giӕQJ Ӄ nÿ sӵSKiW Ӈ QWUL Yj QăQJ 30 suҩ t nҩ P VzYjQJ 3.4 7KjQK ҫ QSK GLQK ӥQJGѭ Fѫ ҧ n cӫ D E Wѫ ҩ P Yj QҧthӇ TX VzYjQJ 31 3.5 .r ғ W  T X D  ÿL Q ҕ K  O ѭѫQ ҕ J  ȕ  J O X F D Q  F R  W U RQ J  W ѫ Q ҩ P Yj ҧ TX Ѵ ғ 35 thӇVzYjQJ 3.6 KhҧQăQJNKiQJ ҭ n cӫ a dӏ ch NKX chiӃ WKr ҕWѫ quҧ QkғPYj 36 thӇVzYjQJ WUrQ(FROL 3.7 ChӍWLrXYLVLQKY ұ WWURQJWѫQ ҩ PVzYjQJQX{LF ҩ y dӏ ch 41 thӇ DANH MӨ&+Ỵ1+ ҦNHĈ Ӗ THӎ 6{ғ KLr ҕX KÕ QK Y H Ѻ Ғ 7r Q KÕ QK Y H Ѻ Ғ Trang 3.1 HӋsӧL Wѫ ҩ P Vz Q YjQJ VDX ҩ \ WƭQK QJj\ WUrQ QX{L F 24 P{LWUѭ ӡng dӏ ch thӇ 3.2 HӋsӧL Wѫ ҩ P Vz Q YjQJ VDX ҩ y lҳ  F WUrQ QJj\ QX{L F 25 P{LWUѭ ӡng dӏ ch thӇ 3.3 HӋsӧL Wѫ ҩ P Vz Q YjQJ VDX ҩ \ WƭQK QJj\ WUrQ QX{L F 27 P{LWUѭ ӡng dӏ ch thӇFyS+NKiF ӯÿ Ӄ n 5,5 QKDX W 3.4 HӋsӧLWѫQ ҩ P VzYjQJ VDXQJj\ ҩ \ WƭQKWUrQ QX{LF 28 P{LWU ѭӡng dӏ ch thӇFyS+NKiF ӯÿ Ӄ n 7,5 QKDX W 3.5 HӋsӧLWѫQ ҩ P VzYjQJ ӡng WUrQP{LWUѭ dӏ ch thӇ 29 3.6 HӋsӧLWѫQ ҩ P VzYjQJ TXDQ Ӈ n vi ViWWUrQNtQK 29 3.7 .r ғ W  T X D K D PO ѭѫQ ҕ J S RO \ V D F F K D U L G H Q J RD L ҕ E D RW U RQ J  Ѵ Ғ Ғ G L F ҕ K  Q X {L  F k ғ \  W ƭ Q K  Y D  O ă ғ F Ғ 33 3.8 Tӫ a polysaccharide ngoҥ LEjR ӫ a dӏ WK{F ch thӇ 3.9 9zQJNKiQJNKX ҭ n cӫ a dӏ ch chiӃ W WѫQ ҩ PD 15 + 0{L ӡng WUѭ PD cҧ i tiӃ n bәVXQJҥ FiP o (PDC+  J NKRDL Wk\ ҥ o  25g; pepton 2g; cao nҩ P PHQJJOXFRVHJQѭ ӟc cҩ Wÿ ӫOtW &iFP {LWUѭ ӡQJ ӧcÿѭ pha vӟi nӗQJÿ ӝNKiFQKDXVDX ҩ p khӱWUQJ ӣÿL ÿyK Ӆ u kiӋ n 121 & WURQJӇ nguӝL SK~Wÿ Yj ҩ y giӕQJ F ӇӣSKzQJO ÿ ҥ nh 25 0C Sau 15 & QJj\WDӕ WKX L Yj ҩ yӣ VLQK nhiӋ VW ӝ105 ÿNK Ӄ nÿkhӕL ӧQJ Oѭ NK{QJ әL Yj Ӄ n ÿ WL KjQKӑFkQWU QJOѭ ӧQJ NK{ b Kh̫RViW ̫QKK˱ ͧng cͯDP{LWU˱ ͥQJGLQKG˱ ͩQJÿ ͇ QVLQKWU˱ ͧng h͏sͫi ṋm VzYjQJ WURQJP{LWU˱ ͥng d͓ ch th͋QX{L ̭y l̷cF BӕWUtWKt Ӌ PQKѭNK QJKL ҧ R ViW ӣQX{L ҩ \WƭQKQKѭQJ F ҩ \ WKu EuQK VDXNKL WDP JLiFÿѭ ӧF ÿHP ҳ c vӟ ÿL i tӕF O ӝÿ  YzQJSK~W WURQJ ӗL VDX YzQJ ÿy lӑc sinh khӕi qua giҩ y lӑc Yj ҩ yӣ V105 C thu sinh khӕLNK{ c Kh̫R ViW ͹̫QK VͧK˱ ng cͯD S+ ͇ Qÿ VLQK ͧng h͏ WU˱ sͫi Vz YjQJ WURQJ P{L WU˱ ͥng d͓ ch th͋QX{L ̭\WƭQK F 3KDP{ LWUѭ ӡng lӓQJFyVLQKWUѭ ӣng cӫ a hӋsӧi cao nhҩ t vӟLS+NKiFQKD 4,5; 5,0; 5,5; 6,0; 6,5; 7,0; 7,5 Yjӕ SKkQSK i 5POYjR EuQKWDPJL 0{L ӡng WUѭ ÿѭ ӧc hҩ p khӱWUQJ ӣ121 0&WURQJYzQJ SK~Wÿ ӇnguӝLYjF ҩ y mӝWOѭ ӧng giӕng nhҩ W ӏ QK ÿ YjR ӇӣSKzQJ ÿ ҥ nh 25 0O C TiӃ Q KjQK ұ n sinh WKX khӕi sau QK 15 QJj\ Sinh khӕLÿѭ ӧc sҩ \NK{ÿ Ӄ n khӕLOѭ ӧQJNK{QJÿ әi ӣ105 0&YjWL Ӄ QKjQKFkQW ӑng Oѭ ӧQJ NK{ BӕWUt WKt Ӌ m vӟi QJKL nghiӋ m thӭc, mӛi nghiӋ m thӭc EuQK WDP 7Kt JLi nghiӋ m lһ p lҥ i lҫ n d Kh̫R ViW ͹̫QK VͧK˱ ng cͯD S+ ͇ Qÿ VLQK ͧng h͏ WU˱ sͫi Vz YjQJ WURQJ P{L WU˱ ͥng d͓ ch th͋QX{L ̭y l̷cF BӕWUt WKtQJKL Ӌ P QKѭ ҧ RViW NK pH ӣQX{L ҩ \ WƭQKQKѭQJ F ҩ \ WKu VDX EuQ N WDPJLiF ӧFÿHP ÿѭ ҳ c vӟ ÿLO i tӕF ӝÿ  YzQJSK~WWURQJ ӗi sau Yz ÿyO ӑc sinh khӕi qua giҩ y lӑFYj ҩ y ӣ105 V0C thu sinh khӕLNK{ 2.3.2 3KѭѫQJ SKiS ӏ nh sinh [iFÿ khӕi NK{ 16 Giҩ y lӑc sҩ y ӣnhiӋ Wÿ ӝ105 C ÿӃ n khӕLOѭ ӧQJNK{QJÿ әi&kQFiFJL ҩ y lӑFÿ Ӈ biӃ t khӕLOѭ ӧng cӫ DFK~QJ'QJFiFJL ҩ y lӑFÿyÿ Ӈlӑc sinh khӕi tӯFiFEuQKW JLiFVDX ҩ \'QJ NKLQX{L Ӊ Xÿ Ӈÿӥ SK tӡgiҩ Fy lӑc Sau chҧ y hӃ W P{L ӡng WUѭ qua giҩ y lӑc sӁÿHPV ҩ y sinh khӕL FQJJL ҩ y lӑc Sҩ y ӣnhiӋ W ӝ105 ÿ &Ӄ nÿkhӕi Oѭ ӧQJNK{QJÿ әi, ÿӇnguӝi rӗi FkQ7tQK ӕL ӧng Oѭ WRiQNK sinh khӕL NK{WK{QJ FKrQK Ӌ ch khӕ O LOѭ ӧng [19] 2.3.3 3KѭѫQJ SKiS ҧ P ÿiQK TXDQ JLi YjF TXDQ Ӌsӧ ViW i nҩ m Vz KuQK YjQJ a ĈiQKJLi ̫m quan b̹ FQJ SK˱˯QJSKiSTXDQ ̷c, tr̩QJWKiLYj ViWPjX ͡ Gj\F ͯa h͏sͫi [3] + HӋsӧL ҥ W ÿ \rX ҫ u: hӋsӧ FL PjX ҳ QJ ӗ WU ng nhҩ ÿ t, mӑc khӓe, thҷ QJ Yj FKL QKiQK Ӆ u, thuҫ ÿ n chӫ ng + HӋsӧL NK{QJ ҥ W \rX ҫ u:ÿhӋsӧ FL әL ÿ PjX Ӄ W ұ m,ÿ Y vӃ t nhҥ W  ӏ FK Fy PjX G YjQJ + HӋsӧL NK{QJ Ӈ Q SKiW NK{QJ Ӌ WUL Qӧ Wѭ ng Fy bung KL sӧi, mү u chӃ t, nhiӉ m vi sinh vұ t b 4XDQViWKuQK ͏sͫi ṋm VzYjQJ WKiLK Lҩ y mӝWӧ tWV i nҩ PGjQ Ӆ XYjR ÿӑWQѭ ӟF JLFyWUrQ PNt nh Cӕÿӏ OD nh sӧi nҩ m WUrQODP bҵ QJNtQK FiFK ҽODP KѫNtQKTXD QK ҥ L WUrQ ӑQ ÿqQ O ӗ QJ n Nhuӝ Fm bҵ ng dung dӏ ch xanh methylen 5- SK~W5 ӱa thuӕc nhuӝm bҵ ng cӗQ6DXÿy ӱa lҥ i vӟLӟ Qѭ c cҩ W/rQNtQK ҧ Q TXDQ ӟ Gѭ i vұ W NtQK ViWWLrXE [6] [ 2.3.4 ;iFÿ ӏ QKWKjQKSK ҫ QGLQKGѭ ӥng quҧthӇYjWѫQ ҩ m VzYjQJ [10], [17] Sӱdө QJ FiF SKѭѫQJSKiS ӇÿiQK JLi hKyD phҫ QGLQK WKjQ VLQK ӥng cӫ aGѭ ÿ hӋ sӧi nҩ m VzYjQJ Yj ҧthӇ TX nҩ m VzYjQJ : - ;iF ӏ QK ÿӝҭ ÿ m cӫ a hӋsӧi nҩ m bҵ QJ SKѭѫQJ ҩ \ NK{ SKiS Ӄ n khӕL ÿV ӧng Oѭ NK{QJ әi ÿ - ;iFÿ ӏ QKKjP ӧng protein Oѭbҵ QJ SKѭѫQJ SKiS %LXUH 17 - ;iFÿ ӏ QKKjP ӧng lipid Oѭ bҵ QJ SKѭѫQJ SKiS 6R[KOHW - ;iFÿ ӏ nh KjP ӧngOѭ tro bҵ QJ SKѭѫQJӃ SKiS n khӕL ӧQJ Oѭ QXQJ NK{QJ әi ÿ ÿ - Ĉӏ QK ӧng Oѭ vitamin B1 bҵ QJ SKѭѫQJ Ǥ nh quang SKiS KX 2.3.5 ;iFÿ ӏ QKGѭ ӧc chҩ WFyWURQJK Ӌsӧi nҩ m, quҧthӇVzYjQJ YjG ӏ ch thӇsau QX{LF ҩ y a Chu̱n b͓m̳u th͵cͯa h͏sͫi ṋm VzYjQJ 7ѫ ҩ P Q QX{L WURQJӡ P{L ng lӓng Yj WUѭ ҧthӇ TXnҩ m Vz YjQJ thu hoҥ FK ӧcÿѭ ÿHP sҩ \ NK{ ӗL [D\ U ӝW WKjQK VDX QJkP Eӟ ÿy i nhӳ YQJ GXQJ WKtFK P{L ӧp rӗi K tiӃ QKjQK F{TXD\ Ӈthu dӏ ch ÿ chiӃ t b 3K˱˯QJ͓ QKO˱ SKiSÿ ͫng polysaccharide ¾ Ĉ͓ QKO˱ ͫng polysaccharide ngo̩LEjR WK{ d͓ FKQX{LF ̭\WƭQKYjO ̷c h͏ sͫi ṋm VzYjQJ [21] Dӏ FKQX{L cҩ \ÿѭ ӧc lӑc bҵ ng giҩ y lӑc, phҫ n qua giҩ y lӑFÿѭ ӧc thu lҥ i Giҩ y lӑc sau lӑFÿѭ ӧc sҩ y ӣ110 0C 4h rӗLÿHPFkQNK ӕLOѭ ӧng Phҫ n dӏ ch lӑFÿHP ÿLÿRWK ӇWtFKWL Ӄ SÿyWKrPO ҫ n thӇWtFKHWKDQROYjRÿ Ӈtҥ o kӃ t tӫ D6DXÿy tiӃ QKjQKO ӑc thu tӫ a Sҩ y tӫ a ӣ45 0C 36 giӡÿѭ ӧc polysaccharide ngoҥ L EjR WK{7L Ӄ Q KjQK FkQSRO\VDFFKDULGH ҵ QJ FkQSKkQ WtFK E KhӕLOѭ ӧng polysaccharide ngoҥ L EjR ӧFWtQKWKHRF{QJWK ÿѭ ӭc sau: mpolysaccharide = msau ±mEDQ ҫ u ÿ mpolysaccharide: KhӕLOѭ ӧng polysaccharide ngoҥ L EjRWK{J msau: KhӕL ӧng Oѭ sau sҩ y gӗm polysaccharide ngoҥ L EjRҩ WK{ y lӑc Yj (g) mEDQ ӧng giҩ y lӑc (g) ҫ u: Khӕ ÿ LOѭ ¾ Ĉ͓ QKO˱ ͫQJȕ -glucan tͳsinh kh͙i qu̫th͋YjK ͏sͫi ṋm VzYjQJ [19] &kQ PJ ӕL NK{ VLQK Yj ӟ NK c cҩ  W ÿHP POQѭ ҩ p ӣnhiӋ KW ӝ121 ÿ 0& iS suҩ W DW WURQJ ӟiSK~W tӕF ӝÿ  6DX YzQJSK~W ÿy O\ WkP WU Y 18 Lӑc qua giҩ y lӑc Dӏ ch lӑF WKXÿѭ ӧc pha trӝn vӟi lҫ n ethanol 96% lҳ c mҥ QK Yj ÿӇTXDÿrP ӣ40&6DXÿyO\WkPY ӟi tӕFÿ ӝYzQJSK~WWURQ ҫ n nәL OrQ ӧc bӓÿL ÿѭ Yj ҫ n huyӅ SK Q SKӟ SKtD Lӧ ÿѭ c ӫvӟiGѭ NaOH 1M ӣ60 0C WURQJK/ѭ ӧQJ JOXFDQ ӧF[iFÿ ӏ nh ÿѭ bҵ QJ SKѭѫQJ ± SKiS sulfuric SKHQRO 3KѭѫQJSKiSSKHQRO ±VXOIXULF ӏ nh glucan [iFÿ dӵDWUrQ Ӌ c hҩ p thө YL tҥ Lӟ Eѭ c VyQJ QP ӫ a phӭc chҩ tF tҥ o bӣL SKHQRO cbohydrate Yj SӱFD dө QJ Pi\ TXDQ phәhҩ p thө , chӑQӟ Eѭ FVyQJ QP ӵQJ ӡng ÿѭ chuҭ Q [k\G JOXFDQÿR ү u SKkQ WtFK ;k\ ӵQJ G ӡng ÿѭ chuҭ n glucan: Pha dung dӏ ch chҩ t chuҭ Q Oj -glucose ' vӟi nӗQJÿ ӝ1mg/ml (1000ppm) Tӯdung dӏ ch chuҭ QWD SKD WKjQK ҭ n vӟL FiF Gm\ nӗQJÿ ӝPJPOPJPOPJPO ҩ y 5ml mӛi nӗQJ ӝchҩ ÿt chuҭ QÿѭD YjR ӑPO FiF ҫ Q 7KrP Oӧ Oѭ t tҩ t cҧO FiF ӑchuҭ On POSKHQRO VDX ҫ QOѭ ӧW YjR ҩ t cҧFiFO ÿyWKrP Wӑ2,5ml dung dӏ ch OH2 SO4 96% CuӕL FQJ ÿR  ÿ{ hҩ ҕ p thөquang hӑc ӣEѭ ӟF VyQJQP Tӯÿѭ ӡng chuҭ Q [k\ ӵng [iF Gӏ QK ÿӧcÿѭ nӗQJ ӝmү ÿX SKkQ jP WtFK ӧng Oѭ + ȕ-JOXFDQ ӧFWtQK ÿѭ WKHRF{QJWK ӭc sau: +jP ӧng Oѭ ȕ-glucan (%) = షయൈ଼ൈଵ଴଴ ௫ሺ ௚ሻ ൈଵ଴ ௄௅ெ௉் ሺ ௚ሻ 7URQJ[OjV ÿy ӕJDP [iFÿ ӏ nh tӯÿѭ ӡng chuҭ n OjK ӋsӕSKD ORmQJ /037ӕ OjNK LOѭ ӧng mү X EDQ ҫ XÿHPSKkQ ÿ WtFK c ;iFÿ ͓ nh ho̩WWtQKNKiQJNKX ̱n cͯa h͏sͫi ṋm YjTX ̫th͋VzYjQJ [34] Sӱdө ng chӫ ng chuҭ n vi khuҭ n E coli, tiӃ Q KjQK ӱkhҧQăQJ WK NKiQJ ҭ n N cӫ a dӏ ch chiӃ t tӯWѫQ ҩ PYjTX ҧthӇWKHRSKѭѫQJSKiSNKX Ӄ FKWiQWUrQÿ ҥ ch, ÿӕi chӭQJNK{QJ ӱdө ng dӏ chV chiӃ t NӗQJÿ ӝchҩ t chiӃ t tҥ R UDYzQJ ҭ n ӭc Y{N chӃsӵVLQKWUѭ ӣng cӫ a vi khuҭ n 19 d Th͵ho̩W WtQK ͙ ng oxL FK KyD ͯa h͏sͫ Fi ṋm Vz YjQJ b̹QJ SK˱˯QJ SK Reducing power [21] Sӱdө QJ SKѭѫQJ SKiS Ӈ5HGXFLQJ kiӇ m tra hoҥ W WtQK ӕ SRZHU QJ R[L FK ÿ Ky Lҫ QOѭ ӧt lҩ y dӏ ch chiӃ W WKHR ӇWtFK Gm\WK VDXPO PO ӗi  VDXÿy 2ml WKrP dung dӏ ch ethanol 96%, 2ml dung dӏ FK Ӌ mÿ phosphate (0,2 M; S+  YjPO NDOLIHUULF\DQLGH ӕng nghiӋ P Yj ӫӣ300 C 30   SK~W7L Ӄ Sÿy WKrPYjR ӛn hӧp ml trichloroacetic K acid 10% rӗLÿHP ӣOLWk tӕFÿ ӝ YzQJSK~WWURQJ ӕLFQJO ҩ y 3ml phҫ SK~W n dung dӏ FKWUrQ Ӆ &X E mһ t pha vӟi ml etKDQRO YjPOIHUULFFKO ӝhҩ p thөcӫ a dung dӏ FK WUrQ ӟi thiӃ t bӏUVY Vis ӣEѭ ӟF VyQJQP ӵc hiӋ Q WѭѫQJ ӵ7K vӟi W mү u kiӇ m chӭQJ 7Kt Ӌ P ÿѭ ӧ QJKL c tiӃ QKjQK ҫ n O AAI = ோ ௦௔௠௣௟௘ ோ ௖௢௡௧௥௢௟ 7URQJ : ÿy AAI Oj chӍsӕchӕQJR[\KyD Rsample OjP ұ W ӝquang ÿ cӫ a mү u Rcontrol OjP ұ Wÿ ӝquang mү u kiӇ m chӭng e 3K˱˯QJ͓ QKWtQKDONDORLG SKiSÿ ¾ ÈSG ͭQJQJX\rQW ̷c th͵cͯa Webb vͣLFiFKWK ͵QK˱VDX Bӝt nҩ m xay nhuyӉ n (10 ±J  YjGXQJG ӏ ch H2SO4 ÿѭ ӧFFKRYjR H ÿXQQK ҽtrong giӡ Lӑc lҩ y dӏ FKÿ ӇthӱnghiӋ m vӟi thuӕc thӱMayer ¾ Thu͙c th͵ÿ͓ QKWtQKDONDORLG Pha thuӕc thӱMayer bҵ QJ FiFK KzD WDQJ +J&O ӟ PO c cҩ W Yj Qѭ WURQJ J , WURQJ ӟc cҩ t Thu  hӛnPO hӧp Qѭ dung dӏ FK Qj\ ҥ L Yj O WKrP ӟc cҩ t Qѭ FKRÿ ӫ100ml NhӓYjL ӑt thuӕ JL c thӱ0D\HU Yj ӏ FK GXQJ DFLG G ORmQJ ӭa alkaloid, nӃ uFy FK Fy DONDORLG Ӂxuҩ t hiӋ n kӃ t tӫ V D PjX ҳ ng hoһ WU F YjQJ ҥ t Cҫ Q QK X Oѭê ӫ a Yu tҥ o W 20 WKjQKFyWK ӇKzDWDQWU ӣlҥ LWURQJOѭ ӧng thuӕc thӱhoһ FKzDWDQE ӣLHWKDQROF ҹ n dung dӏ ch thӱ[4] f 3K˱˯QJ͓ QKWtQKK SKiSÿ ͫp ch̭t saponin 7KtQJKL ͏ m t̩o b͕t: 6DSRQLQ ҥ o FyWtQKW bӑWQrQ ӝt ÿk\OjP nhӳng SKѭѫQJ SKiS Ӈÿӏ QKWtQKV FKtQK[iF ӵhiӋ n diӋ n cӫ a saponin ÿ 3K˱˯QJSKiSWL ͇ QKjQK KzDWDQ ӏ ch chiӃ POG WYjR ӟF POQѭ QyQJ ӑc / YjR ӝt ӕ P ng nghiӋ m 1,6FPYj ӇnguӝL ÿ WKrPQѭ ӟFFKRÿ ӫ10 ml, lҳ c mҥ nh dӑc theo chiӅ u ӕng nghiӋ P WURQJSK~W Ӈ\rQ ӕng nghiӋ P ĈTXDQ ӟp bӑWYj ViWO ÿiQK Ӄ t JLi quҧ N - Bӑt bӅ QWURQJSK~W  - Bӑt bӅ QWURQJSK~W  - Bӑt bӅ QWURQJSK~W +++  * Th͵nghi͏ m Fontan ±Kaudel: Lҩ y mӝWOѭ ӧng 10 ml dӏ ch chiӃ W&KLD Ӆ u2 ÿ ӕng nghiӋ m + ӔQJ WKrPPO+&O1S+ + ӔQJ WKrPPO1D2+ 1S+    Lҳ c mҥ nh dӑc theo chiӅ u ӕng nghiӋ P WURQJSK~W Ӈ\rQ TXDQ Yj ÿViW cӝt bӑt cҧ2 ӕng nghiӋ m + NӃ u cӝt bӑt cҧ2 ӕQJ FDRQJDQJ Ӆ QQKѭ QKDX QKDX Yj ӝ[iF EWKu ÿӏ QKOjFyVDSRQLQ WULWHUSHQRLG + NӃ X S+ FyF ӝt bӑWFDRKѫQ Ӆ u so vӟi ӕQJS+ QKL ӝVѫE [iFÿ ӏ nh OjFyVDSRQLQ [2] VWHURLG g 3K˱˯QJ͓ QKWtQKDFLGK SKiSÿͷX F˯ Lҩ y ml dӏ ch chiӃ t cӗQFKRYjR ӝt ӕng nghiӋ P P 7KrP YjR ӏ ch mӝWtW GXQJ tinh thӇNa2CO3 KѫQK ҽqua ngӑn lӱa NӃ XFyFiFE ӑWNKtV ӫ LOrQW ӯFiFWLQKW Ӈ Na2CO3 WKuN Ӄ t luұ Q OjFyDFLGK ӳX [1] Fѫ 2.3.6 ;iF ӏ nh ÿ chӍWLrXYLVLQKY ұ t hӋsӧi nҩ m VzYjQJ Ĉӏ QK ӧng Oѭ vi sinh vұ t bҵ QJ SKѭѫQJ Ӄ m sӕSKiS khuҭ n lҥ F ÿWUrQ ӡngP{L W ÿһ c Mү u cҫ Q [iF ӏ QK ӧ ÿ F ÿѭSKD ӣnӗ ORmQJ QJ ӝWKtFK ÿ ӧp T\ K YjR ӍWLrX FK YL sinh vұ t sӁFyP{LWUѭ ӡQJÿ һ c hiӋ XNKiF  P{L QKDX WUѭ ӡQJ ӧcÿѭ chӭa WURQJÿƭD 21 SrWUL 0{LWUѭ ӡQJ [iF ӏ nh chӍWLrX ÿ E.coliP{LWUѭ ӡng Macconkey Agar 0{LWUѭ ӡQJ [iF ӏ nh chӍWLrXW ÿ әng vi sinh vұ t hiӃ X NKtP{LWUѭ ӡng LB (pepton: 5g, cao nҩ P PHQJ1D&O ӟc cҩ Wÿ JDJDU ӫ1l) J 0{LWUѭ ӡng [iF ӏ nh chӍ ÿ WLrXW әng nҩ P PHQYj ҩ m mӕFP{LWUѭ Q ӡng Hansen (glucose: 50g, pepton: 10g, K2HPO4 : 3g, MgSO4.7H22 DJDU ӟc cҩ tJQѭ ÿӫ 1l) 6DXNKLFy ү XSKD PORmQJYj ӡng tiӃ Q KjQKJLHR P{LWUѭ ҩ y 1ml mү u YjR F P{L WUѭ ӡQJĈHP ҩ \ ÿƭDÿm QX{L ӣÿL Ӆ u kiӋ nF nhiӋ W ӝ30 ÿ0C 48 giӡ CuӕL FQJ ÿӃ m khuҭ n lҥ c Yj WtQKN Ӄ t quҧWKHRF{QJWK ӭc sau: ‫ܷܨܥ‬ ܰ ‫ܣ‬ሺ ሻൌ ݃ ݊ͳܸ݂ ͳ൅‫ڮ‬൅ܸ݂݊݅݅ 7URQJ ÿy $ sӕ Oj tӃEjR YL ҭ n (khuҭ NKX n lҥ c) 1g mү u 1Oj tәng sӕkhuҭ n lҥ Fÿ Ӄ Pÿѭ ӧFWUrQFiFÿƭDÿmFK ӑn QOj sӕOѭ ӧng ÿƭDF ҩ y tҥ Lÿ ӝSKD ORmQJ ӭi WK 9 thӇ Oj WtFKG ӏ ch mү u (ml) cҩ \ YjRӡng P{LWUѭ IOj ÿӝSKD ORmQJ ӭng WѭѫQJ 2.3.7 3KѭѫQJ QX{LWU SKiS ӗng thӱnghiӋ m a 1X{L ͛ ng ṋ WU m b̹ng gi͙ ng d̩ng l͗ ng YjG ̩ng h̩t CiFW~L3( chӭa 12JJLiWK ӇPQFѭDFDRVX ÿ ӝҭ m 60 -70ÿѭ ӧc tiӋ WWUQJ bҵ ng nӗi hҩ S iS ҩ t Hai VX c{QJ ӭF WK ӧ ÿѭ c bӕWUt ӟi hai Y loҥ i giӕng lӓQJ ҥ Yj ng G hҥ t MӛL F{QJ ӭF Fy WK W~L  ӛL P W~L ҩ y 50g giӕ Fng hҥ t hoһ c 50ml giӕng lӓng ѬѫP ӗngWU nҩ P WURQJ Ӆ u kiӋ Q ÿL WKѭ ӡng cӫ D SKzQJ Ӌ m WKt vӟi nhiӋ W QJKL ӝÿ trung C Chi tiӃ EuQKa t vӅFKăPVyFWK ӡi kǤSKiWWUL Ӈ n sӧLYjTX ҧthӇnҩ m VzWKHR QKѭ FiFQJKLrQ ӭu ÿmWK ӵc hiӋ QWUѭ F ӟFÿk\ [12] b 3K˱˯QJ SKiSÿiQKJLiQăQJVX ̭t sinh h͕ c cͯa ṋm [13],[47] 1ăQJ ҩ t sinh VX hӑc = KhӕLOѭ ӧng nҩ P WѭѫL[NK ӕLOѭ ӧQJ QJX\rQ Ӌ u OL ... VDX /RjL Pleurotus citrinopileatus Chi: Pleurotus Hӑ: Pleurotaceae Bӝ: Agaricales Lӟp: Agaricomycetes 1JjQK ө : Basidiomycotina SK Giӟi nҩ m: Mycota (Fungi) Nҩ m VzYjQJ P citrinopileatus. .. Ho̩WWtQKNKiQJXQJWK˱F ͯa polVDFFKDULGHWDQWURQJQ ͣc ͧṋm Pleurotus citrinopileatus + HiӋ u quҧNKiQJ XQJ ӫ a Sarcoma WKѭ 180 ӣ F chuӝt cӫ D  SROVDFFKD tӯnҩ m P citrinopileatus thӇhiӋ n ӣbҧ ng 1.1&ySKkQÿR... WDQ WURQJQ˱ ͣc WiFK ͳ W ṋm Pleurotus citrinopileatus B̫ng 1.2 Hi͏ u qu̫NKiQJXQJWK˱6DUFRPD ͧchu͡t cͯa SROVDFFKDULGHNK{QJWDQWURQJQ˱ ͣc WUtFKOW ͳṋm P citrinopileatus tFK Polysaccharide